• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.1
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• pp.92-97
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• 2005

Inhibition of inflammatory response, acceleration of basal cell growth, and balanced synthesis of extracellular matrix (ECM) are important in healing of cutaneous open wounds. In order to evaluate the healing effects of water extracts of Radix Astragali (the root of Astragalus membranaceus (Fisch.)) on open wound at early stage, the experimental open wounds were generated on the dorsal sides of SD rats under anesthesia. The boiled-water extracts of Radix Astragali $(100{\mu}l)$, soaked into an occlusive film dressing were applied once a day for eleven consecutive days. The healing process was assessed by measuring macroscopic appearance and wound areas of the open wounds. The molecular aspects of healing process by Radix Astragali extracts were also investigated by Hematoxylin-Eosin (H-E) double staining and immunohistological staining of collagen type I in the healed skin area, implying cell density and linear alignment of the granulation tissue, and ECM synthesis and its remodeling, respectively. The Astragali radix extracts were found to significantly accelerate the cutaneous wound healing by suppressing the inflammation and stimulating the basal cell growth in wounded area, as compared to epidermal growth factor (EGF).

• Archives of Pharmacal Research
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• v.26 no.10
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• pp.855-860
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• 2003

The effect of silver sulfadiazine (SSD) on the proliferation of human dermal fibroblast (HDF) was studied to determine the impact of the drug on the wound healing process and dermal mechanical strength. Human dermal fibroblasts were cultured to 80% confluency using DMEM with 10% FBS and viability of the cell was estimated using neutral red assay. In addition, the $2^{nd}$ degree burn wound was prepared on the anterior part of rabbit ear skin and dressings containing SSD were applied for 96 h. Presence of inflammatory cells and degree of re-epithelialization were investigated in the wound. After 15 day of the induction of burn wounds, the treated area was excised and dermal mechanical strength was quantitatively measured with a constant speed tensiometer. SSD was found to be highly cyto-toxic in cultured HDF cells. The topical application of SSD (2%) could control the infection as evidenced by the lack of accumulation of inflammatory cells in histological evaluation. Therefore, these observations suggested that the impairment of dermal regeneration and decreased mechanical strength of dermal tissue was resulted from the cyto-toxic effect of SSD on dermal cells. Since the decreased mechanical strength may lead to reduction in resilience, toughness and maximum extension of the tissue, the identification of optimum dose for SSD that limits infection while minimizes the cyto-toxic effect may be clinically relevant.

• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.342-347
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• 2005

This study was designed to assess the healing effects of ascorbic acid on full-thickness skin wound in dogs. Four $2.5\pm2.5cm$ full-thickness skin wounds were created bilaterally on the dorsolateral aspect in each of 5 dogs. The ten wounds were treated with ascorbic acid solution in the experimental group, and the ten wounds were treated with $0.9\%$ saline in the control group. The wound healing area was measured every other day for 3 weeks after the wounding. Statistical analysis was conducted with the paired t-test. On the $1^{st},\;3^{rd},\;5^{th},\; 7^{th},\;9^{th},\;11^{th},\;13^{th},\;15^{th},\;17^{th},\;19^{th},\;21^{st}days$, the wound healing rates were $12.6\pm9.6\%,\;9.5\pm9.8\%,\;18.9\pm9.0\%,\;39.2\pm5.4\%,\;62.2\pm4.6\%,\;86.9\pm1.9\%,\;92.9\pm1.8\%,\;96.6\pm1.6\%,\;99.1\pm0.5\%,\;99.8\pm0.2\%,\;99.9\pm0.1\%$ in the experimental group, and $9.1\pm6.0\%,\;9.2\pm6.0\%,\;12.2\pm5.0\%,\;41.3 \pm6.3\%,\;54.2\pm9.2\%,\;81.0\pm2.6\%,\;85.7\pm2.8\%,\;92.3\pm1.5\%,\;96.1\pm0.7\%,\;98.0\pm0.8\%,\;99.5\pm0.2\%$ in the control group On the $15^{th}$ day, the wound closed above $99\%$ in 2 dogs of the experimental group. On the $17^{th}$ day, the wound closed above $99\%$ in 3 dogs of the experimental group. On the $19^{th}$ day, the wound closed above $99\%$ in 5 dogs of the experimental group, and 2 dogs of the control group. On the $21^{st}$ day, the wound closed above $99\%$ in 5 dogs of the experimental group, and 4 dogs of the control group. The wound healing rate of the experimental group was $2\~4$ days faster than that of the control group. The tensile strength was $4,169\pm1,107g/cm^2$ in the experimental group, and $2,438\pm637g/cm^2$ in the countrol group on the$21^{st}$ day after wounding. Tensile strength was significantly higher in the experimental group than in the control group (p<0.05). The histopathological findings of the experimental group were similar to those of the control group on the $1^{st},\;3^{rd}\;and\;5^{th}$days. Inflammation and revascularization of the experimental group were similar to those of the control group on the $9^{th},\;13^{th}\;and\;21^{st}$days. The formation of collagen and reepithelialization were more developed the experimental group than in the control group on the $9^{th},\;13^{th}\;and\;21^{st}$days. Significantly higher wound healing rate, tensile strength and better histopathological findings were observed in the experimental group than in the control group. These results suggest that the topical application of ascorbic acid on full-thickness skin wounds can promote wound healing process.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.5
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• pp.481-489
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• 2000

This research was focused on overall examination of tissue alteration, wound healing promotion. After the hair on the dorsal surface was shaved, $5{\times}5mm$ oval skin defect was formed. Experimental wounds of right side were irradiated on every day for 90 second with Ga-Al-As semi-conductor laser. Left side wounds served as control group. The rats were sacrificed on the 1st, 3rd, 5th, 7th, 14th, 21th day. For light microscopically, parafin section were stained with H&E, MT. The outcomes were as follows : 1. On 1st day, experimental and control group were seen acute inflammatory cell infiltration, edema. 2. On the 3rd days, both groups were seen crust development, collagen, blood vessel proliferation. 3. On the 5th days, experimental group were reduced edema and inflammatory cell infiltration than control group. 4. On the 7th days, both groups were observed edema, inflammatory cell infiltration disappearance and keratinocytes motility from wound defect. 5. On the 14th days, experimental group appeared collagen, blood vessel proliferation and hair follicle than control group. 6. On the 21th days, both groups were seen normal status re-epithelization. According to the above results, The wound-healing stimulated by laser radiation involves an increased rate of epithelial growth. LLLT was confirmed that it has fibroblast, blood vessel proliferation, influence initial wound healing process.

• The Korean Journal of Pain
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• v.7 no.1
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• pp.74-77
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• 1994

Since its introduction, low level lasers have been utilized in many varied clinical applications for the treatment of musculoskeletal and body surface lesions. Due to the laser beams specific characteristic-coherence, monochromaticity and unidirectionality, without adverse reaction with neighbouring tissue, physicians have used it with relative ease. We observed accelerating effects of healing process of some intractable skin wound, improvement of graft survival, as well as reduction of pain on ulcerating tissues, by treating twice weekly with low level Helium-Neon(He-Ne) and Infrared(IR) laser. The laser's energy density was set at $1\;J/cm^2$ and a fixed frequency of 600 Hz by continuous scanning. Irradiating time was calculated according to the cross diameter of the wound. Further clinical work will be required to evaluate the accelerated healing processes by biostimulating mechanisms of laser ray, especially for the intractable(diabetic) skin ulceration.

• Journal of the Korean Burn Society
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• v.23 no.1
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• pp.20-24
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• 2020

Diabetic patients have an increased risk of burn injuries on foot. Because of their diabetic neuropathy, they could contact with hot water or warming device without being aware of it. Split-thickness skin graft (STSG) is successful in treatment of various wound types; however, donor site wounds are sometimes problematic, and complications such as pain and impaired healing often occur. Although, donor site wounds in healthy young individuals can rapidly heal without complications, the wound-healing capacity of elderly patients or those with a comorbidity has been reported to be low. The dermatome is the most commonly used tool because it can harvest a large skin graft in one attempt. However, it is difficult to harvest tissues if the area is not flat. Furthermore, because the harvested skin is usually rectangular, additional skin usually remains after skin grafting. Therefore, use of razor blade and fragmented STSG on a large defect area is advantageous for harvesting a graft with a desired size, shape, and thickness. From January 2018 to July 2018, fragmented STSG was used in 9 patients who suffered from burn induced open wound on foot with diabetic neuropathy. With this approach, healing process was relatively rapid. The mean age of patients was 70 (57~86 years) and all of 9 patients had diabetes mellitus type 2. In all patients, the skin graft on the defect site healed well and did not result in complications such as hematoma or seroma.

• The Journal of the Korean dental association
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• v.13 no.2
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• pp.139-145
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• 1975

• The Journal of the Korean dental association
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• v.21 no.9 s.172
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• pp.741-753
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• 1983

• The Journal of Korean Physical Therapy
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• v.22 no.3
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• pp.87-92
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• 2010

Purpose: The purpose of this study was to investigate the effect of electrical stimulation (ES) on the wound closure rate, collagen deposition, and TGF-${\beta}$1 mRNA expression in skin wound of rat. Methods: Twenty male Sprague-Dawley rats (222~271 g) were randomly divided into ES (n=10) and control group (n=10). The ES group received a cathodal stimulation with 50 V at 100 pps for 30 minutes for 7 days, while the control group was not given electrical stimulation. The wound closure rate, collagen density and TGF-${\beta}$1 mRNA ratio were measured. Results: The mean wound closure rates in the ES and control groups were $83.79{\pm}16.35$% and $51.57{\pm}17.76$%, respectively (p<0.001). The collagen density in the ES and control groups were $46.67{\pm}10.68$% and $25.03{\pm}13.09$%, respectively (p<0.001). The TGF-${\beta}$1 mRNA ratio in the ES and control groups were $1.35{\pm}0.60$ and $0.63{\pm}0.30$, respectively at 6 hours post-wound (p<0.01) and $1.69{\pm}0.47$ and $1.32{\pm}0.28$, respectively, at 7 days post-wound (p<0.05). Conclusions: ES accelerated the wound closure rate of skin incision wounds and was accompanied by an increase in collagen deposition in the regenerating dermis. In addition, ES increased TGF-${\beta}$1 mRNA expression during wound healing process. These findings suggest that ES may activate TGF-${\beta}$1 expression, and may increase synthesis activities of fibroblasts in regenerating skin wounds in rats.

• Journal of Radiation Industry
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• v.4 no.4
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• pp.365-372
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• 2010

Irradiation has been recognized as a highly suitable tool to aid in the formation of hydrogel. The radiation process has various advantage, such as easy process control and the lack of necessity for initiators and crosslinker. In this study, the hydrogel containing the citrus fermentation gel for the wound healing were successfully synthesized. The strength of hydrogel was increased as a function of a increasing the concentration of citrus and the irradiation doses. In addition, this hydrogel have been evaluated by the cytotoxicity and animal experiment.