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Effect of Topically Applied Silver Sulfadiazine on Fibroblast Cell Proliferation and Biomechanical Properties of the Wound  

Lee, Ae-Ri-Cho (College of Pharmacy, Duksung Womens University)
Moon, Hee-Kyung (College of Pharmacy, Duksung Womens University)
Publication Information
Archives of Pharmacal Research / v.26, no.10, 2003 , pp. 855-860 More about this Journal
Abstract
The effect of silver sulfadiazine (SSD) on the proliferation of human dermal fibroblast (HDF) was studied to determine the impact of the drug on the wound healing process and dermal mechanical strength. Human dermal fibroblasts were cultured to 80% confluency using DMEM with 10% FBS and viability of the cell was estimated using neutral red assay. In addition, the $2^{nd}$ degree burn wound was prepared on the anterior part of rabbit ear skin and dressings containing SSD were applied for 96 h. Presence of inflammatory cells and degree of re-epithelialization were investigated in the wound. After 15 day of the induction of burn wounds, the treated area was excised and dermal mechanical strength was quantitatively measured with a constant speed tensiometer. SSD was found to be highly cyto-toxic in cultured HDF cells. The topical application of SSD (2%) could control the infection as evidenced by the lack of accumulation of inflammatory cells in histological evaluation. Therefore, these observations suggested that the impairment of dermal regeneration and decreased mechanical strength of dermal tissue was resulted from the cyto-toxic effect of SSD on dermal cells. Since the decreased mechanical strength may lead to reduction in resilience, toughness and maximum extension of the tissue, the identification of optimum dose for SSD that limits infection while minimizes the cyto-toxic effect may be clinically relevant.
Keywords
Silver-sulfadiazine; Fibroblast; Dermis; Wound healing; Burn;
Citations & Related Records

Times Cited By Web Of Science : 12  (Related Records In Web of Science)
Times Cited By SCOPUS : 16
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1 Beaubien, J., Boisjoly, H. M., Gagnon, P., and Guidoin, R., Mechanical properties of the rabbit cornea during wound healing after treatment with epidermal growth. Can. J. Opthalmol., 29, 61-65 (1994)
2 Brown, G. L., Curtsinger, L. J., White, M., Mitchell, R. O., Pietsch, J., Nordquist, R, Fraunhofer, A., and Schultz, G. S., Acceleration of tensile strength of incisions treated with EGF and TGF-beta. Ann. Surg., 208, 788-794 (1988)   DOI   PUBMED   ScienceOn
3 Cho Lee, A. R., Effect of silver sulfadiazine on the skin cell proliferation and wound healing process in Hairless mouse 2nd degree burn model. J. Kor. Pharm. Sci., 32, 113-117 (2002)
4 Leitch, I. O., Kucukcelebi, A., and Robson, M. C., Inhibition of wound contraction by topical antimicrobials. Aust. N. Z. J. Surg., 63(4), 289-293 (1993)   DOI   PUBMED
5 Murphy, G. F., Histology of the skin, In Elder, David, (Ed.) Lever's Histology of the Skin, 8th Ed. Lippincott-Raven Publishers, phildelphia, pp. 5-49, (1997)
6 McCauley, R. L., Li, Y. Y., Poole, B., Evans, M. J., Robson, M. C., Heggers, J. P., and Herndon, D. N., Differential inhibition of human basal keratinocyte growth to silver sulfadiazine and mafenide acetate. J. Surg. Res., 52(3), 276-285 (1992)   DOI   ScienceOn
7 Reddy, G. K., Stehno-Bittel, L., Enwemeka, C. S., Matrix remodeling in healing rabbit Achilles tendon. Wound Repair Regen., 7(6), 518-527 (1999)   DOI   ScienceOn
8 Wright, J. B., Lam, K., and Burrell, R. E., Wound management in an era of increasing bacterial antibiotic resistance : a role for topical silver treatment. Am. J. Infect. Control., 26, 572-577 (1998)   DOI   ScienceOn
9 Piscatelli, S. J., Michaels, B. M., Gregory, P., Jennings, R. W., Longaker, M. T. Harrison, M. R., and Siebert, J. W., Fetal fibroblast contraction of collagen matrices in vitro: the effects of epidermal growth factor and transforming qrowth factor-b. Ann. Plast. Surg., 33, 38-45 (1994)   DOI   PUBMED   ScienceOn
10 Fox, C. L., Topical therapy and the development of silver sulfadiazine. Surg. Gynecol. Obstet., 157, 82-88 (1983)   PUBMED
11 Wilgus, T. A., Vodovotz, Y., Vittadini, E., Clubbs, E. A., and Oberyszyn, T. M., Reduction of scar formation in full-thickness wounds with topical celecoxib treatment. Wound Repair Regen., 11(1), 25-34 (2003)   DOI   ScienceOn
12 Davis, P. A. and Wasteli, C., A comparison of biomechanical properties of excised mature scars from HIV patients and non-HIV controls. Am. J. Surg., 180, 217-222 (2000)   DOI   ScienceOn
13 McCauley, R., Chopra, R. D., Herndon, D., and Robbson, M., Cytoprotection of human derrmal fibroblasts against silver sulfadiazine using recombinant growth factor. J. Surg. Res., 56, 378-384 (1994)   DOI   ScienceOn
14 Cho Lee, A. R., Yoon, J. J., Lim, H., Park, Y., Jung, K. H., and Lee, J., Preparation of alginate microscope and collagen sponge for controlled release and its application to wound dressing. Proceed, Inl. Symp. Control. Rel. Bioact. Mater., 26, 471-472 (1999)
15 Gear, A. J., Hellewell, T. B., Wright, H. R., Mazzarese, P. M., Arnold, P. B., Rodeheaver, G. T., and Edlich, R. F., A new silver sulfadiazine water soluble gel. Burns. 23(5), 387-391 (1997)   DOI   ScienceOn
16 Fuller, F. W., Parrish, M., and Nance, F. C., A review of the dosimetry of 1% silver sulfadiazine cream in burn wound treatment, J. Burn. Care. Rehabil., 15(3), 213-223 (1994)   DOI   ScienceOn
17 Smoot, E. C., Kucan, J. O., Roth, A., Mody, N., and Debs, N., In vitro toxicity testing for antibacterials against human keratinocytes. Plast. Reconstr. Surg., 87, 917-924 (1991)   DOI   ScienceOn
18 Berthod, F., Germain, L., Li, H., Xu, W., Damour, O., and Auger, F. A., Collageb fibril network and elastic system remodeling in a reconstructed skin transplanted on nude mice. Matrix Biology., 20, 463-473 (2001)   DOI   ScienceOn
19 Cooper, M. L., Laxer, J. A., and Hansbrough, J. F.,The cytotoxic effects of commonly used topical antimicrobial agents on human fibroblasts and keratinocytes. J. Trauma., 31(6), 775-782, discussion 782-784 (1991)   DOI   PUBMED   ScienceOn