• 대한간호학회지
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• 제28권1호
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• pp.47-59
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• 1998

The purpose of this study was to identify calls, roles and attitudes of the Christian medical staff in a modern medical system for holistic healing through belief in God's healing methods and God's view about medical treatment. The meaning of healing in the Bible is derived from Rapha in the Old Testament, it's meaning is 'heal wound', 'restore to original condition', 'repair', 'console' and 'be heal'. In the New Testament, the meaning of healing is 'to serve' and 'be in one's service' derived from Therapuein and preserve', 'rescue', 'save a life from death' derived from Sozo. The term of soteriology originated from Sozo. Therefore the meaning of the healing in the Bible is restoring original completeness to the same as Cod's characteristics. The meaning of disease is physical, psychological, social and spiritual imbalance or disharmonious. Disease is usually depravity from moral life to immoral life and abnormal life process with accompaning specific symptoms. Medical staff were called to God's work. recognized God's will for them, and absolutely leaned on God's power to intervene and work above spatial-temporal transcendently. They use spiritual power with medical treatment skills, help sick people to possibly have dynamic and individual relation with God and help to maintain their well-being and complete healing. Attitudes of medical staff were compassion and love, virtue of modesty, strong and daring, patience with belief, healing with God's word, using spiritual insight, play. using medical knowledge and techniques, continuing spiritual training, laying on of hands and repentance.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권4호
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• pp.369-372
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• 2000

The purpose of this study was to investigate the distribution pattern of CGRP immunoreactive nerve fibers in the healing mucosa of extracion wound. Maxillary 1st molars of rats were extracted. All extraction sites and adjacent tissues of 3 groups of rats(1-week, 2-week and 4-week groups) were removed en bloc and processed for immunostaining and were subjected to light microscopic examination. The results obtained were as follows; In 1-week group, there was no difference in the distribution pattern of CGRP immunoreactive nerve fiber between epithelial margin adjacent to extraction socket and normal gingival epithelium. In 2-week group, some CGRP-immunoreactive nerve fibers were seen in epithelial layer. In 4-week group, many intercellular CGRP immunoreactive nerve fibers were abundant in all layers of immature epithelium characterized by scab on the mucosa and thick keratinized cell layer with irregular surface. Intraepithelial CGRP immunoreactive nerve fibers were reduced to normal level in adjacent mature epithelium. These results suggest that density of CGRP immunoreactive nerve fibers are increased transiently in epithelium during reepithelialization process and CGRP released from these nerve fibers may play an important role in the reepithelialization in the wound healing.

• Applied Microscopy
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• 제28권3호
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• pp.379-390
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• 1998

Dermal wound healing responses in the skin of the toad, Bombina orientalis, were examined using transmission electron microscopy. At 12 hours after wounding, debridement and collagenolysis occurred in damaged dermis. Histocyte has a large nucleus and long cytoplasm process. Phagocytic vesicle and lysosome were observed in the cytoplasm. Damaged blood cells were transformed spindle to irregular shape. Autolysis was observed in their cytoplasm. Histocytes are found in poly-band. The irregularly shaped nucleus is located peripheral region in cytoplasm. At 2 days after wounding, partial aggregation of blood cells is observed. Phagocytic, activity is observed in histocyte and collagenolytic collagen fibers are scattered. Fibroblast is observed in the dermis at 3 days after wounding. Clusters of ribosomes and some short cisternae of rough-surfaced endoplasmic reticulum are found in the cytoplasm. In histocyte at 7 days post wounding, various size granules composed of moderately dense material are found the cytoplasm. In this period histocyte is round to rod in profile, with slender processes projecting from the surface. At 7 day after injury, it was observed that formation of connective tissue fibers and amorphous ground substance in regenerating skin.

• 한국산학기술학회논문지
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• 제16권8호
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• pp.5336-5342
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• 2015

삶의 질이 향상됨에 따라 환자의 흉터 치료 수요는 증가하고 있는 반면, 흉터 개선 제품은 대부분 수입 제품에 의존하고 있는 실정이다. 엔케팔린은 신경말단에서 분비하는 신경 펩타이드의 한 종류이다. 피부세포와 신경세포는 배엽 발달 과정 중 공통적으로 외배엽에서 유래하며, 피부세포 막에서 신경세포에서 발현되는 신경펩타이드 수용체 즉, 오피오이드 수용체(Opioid Receptor)가 발현된다. 오피오이드 수용체는 뮤-(${\mu}$), 델타-(${\delta}$), 카파-(${\kappa}$) 세 종류가 있으며, 각각을 MOR, DOR, KOR라고 부르고, 델타-오피오이드 수용체는 피부에서의 상처 치유(Wound-healing)에 직접 관여하는 것으로 보인다. 본 논문에서는 엔케팔린의 Alanine Scan 방법을 이용하여 엔케팔린 유도체를 합성하여, 피부세포내 안전성과 Cell migration assay를 통한 In vitro 상처 치유 촉진 효능, 프로 콜라겐 합성능력 및 보습효과를 실험을 통해 검증하였고, 피부 상처 마우스 모델에서 엔케팔린 유도체의 상처 치료 효과를 확인하였다. 그 결과 엔케팔린 유도체 AGGFL(AS10) 및 YGGAL(AS13)이 상처 치유 효능, 프로 콜라겐 합성증가, 보습 관련 유전자 발현 증가를 확인하였고, 특히 AS13이 피부 상처 마우스 모델에서 뛰어난 상처치료 효과를 확인하였다.

• Restorative Dentistry and Endodontics
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• 제14권2호
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• pp.49-64
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• 1989

The purpose of this study was to evaluate the effect of several materials on the healing process of apical wound. Sixteen mandibular premolars obtained from 4 healthy dogs were used for this study. Under general anesthesia, the pulpal chamber of each tooth was opened and the pulps were extirpated. The root canals were then instrumented with H-file and irrigated with physiologic saline solution ; the apices were purposely perforated and enlarged with the engine K-reamer. In the experimental groups, apical wounds were filled with one of calcium hydroxide, hydroxylapatite, and tricalcium phosphate materials, mixture of each materials and physiologic saline solution, with a lentulo spiral. In the control group, apical wounds were not filled with any material. All the root canals were filled by the lateral condensation technique with gutta-percha cone and ZOE sealer. The access opening of all the teeth were closed with amalgam. On the 10, 20, 40 and 60th day after experiment, experimental animals were sacrificed. Segments of jaws, each containing one tooth, were fixed in 10% formalin solution and decalcified in Plank-Rychlo solution. The specimens were embedded in paraffin and serially sectioned to an average thickness of $6{\mu}m$. The sections were stained by hematoxylin-eosin and Masson's trichrome stain method and examined under light microscope. The results were as follows : 1. In the experimental groups, the new bone formations were observed in apical wounds. 2. Fourty days later, apical wounds were healed by granulation tissue in the experimental groups, but were not healed by granulation tissue in the control group, and the healing process of experimental groups were more rapid than that of control group. 3. Sixty days later, chronic inflammation disappeared in the experimental groups, and the materials used showed biologic affinity to the periapical tissue. 4. In all the groups, the resorption of cementum appeared on the 10th and 20th day after experiment, and the deposition of cementum appeared on the 40th and 60th day after experiment, especially showing narrowness of apical foramen due to newly formed cementum in calcium hydroxide group. 5. Calcum hydroxide and tricalcium phosphate particles were gradually resolved, but hydroxylapatite particles were not resolved through the experimental period.

• 한국어류학회지
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• 제29권1호
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• pp.22-31
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• 2017

메기, Silurus asotus의 외과적 시술시 상처 회복 및 스트레스 반응을 알아보기 위하여, 수술 과정에 의한 스트레스 호르몬의 변화 양상과 회복과정을 분석하였다. 수술 후 clove oil (1,000 ppm) 마취한 군의 생존율은 수술 후 3~42일 동안 약 90%를 보였으며 무마취군은 약 74%를 보였다. 즉, 마취를 한 후 수술하였을 때 무마취군 보다 생존율이 높게 나타났다. Clove oil로 마취한 군의 혈장 cortisol 농도가 무마취군 보다 높았으며(P<0.05) 아울러, clove oil로 마취한 군의 혈장 lactic acid 농도 역시 무마취군 보다 높았다(P<0.05). 수술 후 14일과 28일째는 아직 봉합사의 흔적이 남아 있었고 움직임도 활동적이지 않았지만 35일째 되었을 때는 봉합자국은 사라졌고 수술 후 42일에는 봉합사도 관찰되지 않았으며, 봉합 상처도 회복이 되었다. 따라서 메기의 외과시술시, 마취를 한 후 수술하였을 때 무마취군 보다 생존율이 높게 나타났고, 수술 후 받는 스트레스 양도 적다는 것을 알 수 있다. 즉, 본 연구의 메기에서의 외과적 상처 회복은 마취제 사용시 성공적으로 이루어짐을 보였다.

• 생명과학회지
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• 제29권3호
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• pp.382-393
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• 2019

상처 치료는 전세계 인류에 영향을 미치는 보건 산업계의 관심사다. 당뇨병과 같은 대사증후군 유병률 증가로 상처에 의한 합병증의 위험이 높아지고 상처치유의 복잡함 때문에 상처의 치료와 관리가 어렵다. 전통적 상처 드레싱은 제한된 보호기능을 제공하며, 상처 드레싱의 치료 능력을 향상시키기 위해 생체고분자 기반의 드레싱들이 개발되고있다. 생체고분자는 생분해성이 뛰어나고 생체적합성이 좋으며 효과적인 상처 관리에 중요한 항균, 항염증, 지혈, 세포증식, 혈관성 활동 등 다양한 효과가 있다. 키토산, 셀룰로오스, 콜라겐, 히알루론산, 알긴산 등의 여러 생체고분자가 이미 상처치유제로 활용되고 있으며 생체고분자를 다른 고분자, 생체활성 분자 및 약물과 결합하여 생리학적 문제 없이 흉터를 최소화하는 새로운 상처 드레싱이 개발되고 있다. 본 논문에서는, 향후의 연구와 활용을 위한 현재의 생체고분자의 상처처리에 대해 알아보았다.

• Archives of Plastic Surgery
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• 제34권4호
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• pp.420-425
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• 2007

Purpose:The mechanism of scar formation is not fully understood. Fibroblast is an important cell in wound healing process. We experienced a patient who was taking progesterone orally. Upper blepharoplasty was performed on her but, wound healing was delayed. We hypothesized that progesterone was the cause of delayed wound healing and fibroblast proliferation inhibition. We investigated the effect of progesterone in vitro on human dermal fibroblasts to study the effects on fibroblast proliferation. Methods: Human dermal fibroblasts from four persons were cultured initially. Progesterone is mixed to them at various concentrations, and fibroblast cell count was measured by MTT assay method at 570 nm. We confirmed that progesterone has some inhibitory effect on fibroblast proliferation and maximal inhibitory concentration of progesterone was determined. Then fibroblasts from a total of nineteen persons were cultured and the effects of progesterone were studied. Results: The initial study showed the maximal inhibitory concentration of progesterone to be $50{\mu}g/ml$. The main study showed that progesterone had 70.9% inhibitory effect on human dermal fibroblast in vitro. Conclusion: Progesterone has inhibitory effect on cultured human dermal fibroblast proliferation in vitro.

• Archives of Plastic Surgery
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• 제49권3호
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• pp.462-470
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• 2022

Background Reactive oxygen species cause serious damage to the physiological function of tissues. Determination of total antioxidant capacity of skin tissue is one of the determinants of damaged tissue function. Mast cells (MCs) are one of the groups of cells that are invited to the site of injury. The healing process begins with the rapid release of various types of MCs' intermediate factors at the site of injury. Bone marrow mesenchymal stem cell (BMMSC) production and secretion have been shown to regenerate the skin. The aim of this research was to evaluate the wound-healing and antioxidant effects of BMMSCs per MCs. Methods Fifty-four albino Wistar male rats were divided into three groups: (1) nonsurgery, (2) surgery, and (3) surgery + BMMSCs. Groups 2 and 3 were operated with a 3 × 8 cm flap and in group 3, cell injections (7 × 10⁹ cell injection at the time of surgery) were performed. After days 4, 7, and 15, percentage of the surviving tissue, histological characteristics, superoxide dismutase (SOD) activity, and amount of malondialdehyde (MDA) were measured in the groups. For results, Graph Pad Prism 8 software was used, and data were analyzed and compared by analysis of variance and Tukey test. Results BMMSCs' application decreased the amount of MDA, increased SOD activity and survival rate of the flaps, and improved the histological characteristics. Conclusion This study revealed the protective effects BMMSCs alongside MCs against oxidative stress on the survival of the flaps. However, for clinical use, more research is needed to determine its benefits.

• Archives of Plastic Surgery
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• 제34권3호
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• pp.305-313
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• 2007

Purpose: Wound healing is a result of complex processes whose components, such as cells, extracellular matrix, proteolytic enzymes, and their inhibitors receive effects from immune compartments, cytokines, chemokines, and growth factors. Impairment of normal physiologic response to wounding makes nonhealing chronic wounds. Wound infection and exacerbated proteolytic process may induce uncontrolled tissue degradation or exudates formation, which may result in the development of a nonhealing chronic wound. Thus proper management of wound infection and exudates is critical to prevent and treat nonhealing wound. The aim of this study is to evaluate effects of Aquacel AG, silver-containing carboxymethylcellulose dressing on treatment for exudative infected wound. Methods: The study included 31 patients with nonhealing wound. Wound was dressed with Aquacel AG. The effect of dressing was investigated by serial bacterial culture and wound exudates assessment. Each infection and exudates control time was determined and statistically analyzed. Results: Wound infection and exudates were effectively managed using Aquacel AG dressing. Mean infection and exudates control time were $3.4{\pm}1.2$ and $5.7{\pm}1.4$ weeks, respectively. Statistical analysis of the data indicated that infection control time correlated positively to age and exudates control time (p<0.05). Conclusion: There is as yet no ideal dressing for the topical treatment of chronic nonhealing wound. But silver-containing carboxymethylcellulose dressing can be used effectively for exudative, infected nonhealing wound.