Browse > Article

Stress Response and Wound Healing of Surgical Incisions in Far Eastern Catfish, Silurus asotus  

Gi, Hyun Woo (Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University)
Lee, Tae Ho (Department of Marine Bio-Materials and Aquaculture, Pukyung National University)
Park, In-Seok (Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University)
Publication Information
Korean Journal of Ichthyology / v.29, no.1, 2017 , pp. 22-31 More about this Journal
Abstract
To find out the stress response and wound healing of surgical incisions, we analyzed the change of stress hormone and the healing process in far eastern catfish, Silurus asotus. The survival rate of the clove oil (1,000 ppm) anesthesia group after surgery was about 90% in 3~42 days, while no anesthesia group showed about 74%. Thus, the survival rate after anesthesia group was higher than that of the no anesthesia group. The plasma cortisol concentration of the clove oil anesthesia group was higher than that of the no anesthesia group (P<0.05). In addition, the plasma glucose concentration of clove oil anesthesia group was higher than that of the no anesthesia group (P<0.05). The plasma lactic acid concentration of clove oil anesthetized group was higher than that of the not anesthetized group (P<0.05). On the 14 days and 28 days after surgery, there were still stitching fiber of suture and swimming behavior was not active. On the 35 days after surgery, trace of the suture has almost disappeared on the outside of the fish. Finally, 42 days after surgery, stitching fiber was not visible, and the sutured wounds were distinctly recovered. The results of this study showed that the anesthesia group showed higher survival rate and received less postoperative stress than that of the no anesthesia group. Surgical wound healing in the catfish of this study was shown to be successful when using anesthetics.
Keywords
Anesthesia; far eastern catfish; stress response; surgical incision; wound healing;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Marty, G.D. and R.C. Summerfelt. 1990. Wound healing in channel catfish by epithelialization and contraction of granulation tissue. Trans. Amer. Fisher. Soc., 119: 140-150.
2 McDonald, D.G. and C.L. Milligan. 1997. Ionic, osmotic and acid base regulation in stress. In: Iwama, G.W., A.D. Picking, J.P. Sumpter and C.B. Schreck (eds.), Fish stress and Health in Aquaculture. Cambrige: University Press, U.K., pp. 119-144.
3 McGee, G.S., J.M. Davidson, A. Buckley, A. Sommer, S.C. Woodward, A.M. Aquino, R. Barbour and A.A. Demetiou. 1988. Recombinant basic fibroblast growth factor accelerates wound healing. J. Surg. Res., 45: 145-157.   DOI
4 Munck, A., P.M. Guyre and J. Holbrool. 1984. Physiological functions of glucocoricoid in stress and their relationship to pharmacological action. Endoc. Rev., 5: 25-34.   DOI
5 Park, I.-S. and J.H. Im. 2001. Determination of the temperature-dependent index of mitotic interval for chromosome manipulation in far eastern catfish, Silurus asotus. Korean J. Ichthyol., 13: 85-88.
6 Park, I.-S., J.H. Im, J.-H. Kim and D.S. Kim. 1998b. Wound healing of surgical incisions in Rhynchocypris oxycephalus. J. Aquacult., 11: 167-172. (in Korean)
7 Park, I.-S., J.H. Kim, J.B. Jung and J.H. Im. 1998a. Effect of lidocaine as an anaesthetic on Rhynchocypris oxycephalus and R. Steindachneri. J. Aquacult., 11: 59-66. (in Korean)
8 Park, M.O., S.-Y. Im, D.-W. Seol and I.-S. Park. 2009. Efficacy and physiological responses of rock bream, Oplegnathus fasciatus to anesthetization with clove oil. Aquaculture, 287: 427-430.   DOI
9 Raabo, E. and T.C. Terkildsen. 1960. On the enzymatic determination of blood glucose. Scand. J. Clinic. Lab. Invest., 12: 402-407.   DOI
10 Roubal, F.R. and A.M. Bullock. 1988. The mechanism of wound repair in the skin of juvenile Atlantic salmon, Salmo salar L., following hydrocortisone implantation. J. Fish Biol., 32: 545-555.   DOI
11 Tomasso, J.R., K.B. Davis and N.C. Parker. 1980. Plasma corticosteroid and electrolyte dynamics of hybrid striped bass (white bass x striped bass) during netting and hauling stress. Proceed. World Maricult. Soc., 11: 303-310.
12 Schreck, C.B. 1981. Stress and compensation in teleostean fishes: response to social and physical factors. In: Pickering, A.D. (ed.), Stress and Fish. Academic Press, London, U.K., pp. 295-321.
13 Selye, H. 1973. The evolution of the stress concept. American Scientist, 61: 692-699.
14 Summerfelt, R.C. and L.S. Smith. 1990. Anesthesia, surgery, and related techniques. In: Schreck, C.B. and P.B. Moyle (eds.), Methods for fish Biology. American Fisheries Society, Bethesda, Maryland, U.S.A., pp. 213-272.
15 Walsh, C.T. and B.C. Pease. 2002. The use of clove oil as an anaesthetic for the long finned eel, Anguilla reinhardtii (Staindachener). Aquacult. Res., 33: 627-635.   DOI
16 Waterstrat, P.R. and L. Pinkham. 2005. Evaluation of eugenol as an anesthetic for the American lobster, Homerus americanus. J. World Aquacult. Soc., 36: 420-424.
17 Park, M.O., W.J. Hur, S.Y. Im, D.S. Seol, J.H. Lee and I.-S. Park. 2008. Anaesthetic efficacy and physiological responses to clove oil-anaesthetized kelp grouper Epinephelus bruneus. Aquacult. Res., 39: 877-884.   DOI
18 Adzick, N.S., M.R. Harrison, P.L. Glick, J.H. Becksted, R.L. Villa, H. Scheuenstuhl and W.H. Goodson. 1985. Comparison of fetal, new-born, and adult wound healing by histologic, enzyme histo-chemical, and hydroxyproline determinations. J. Pediat. Surg., 20: 315-319.   DOI
19 Weber, R.A., J.B. Peleteiro, L.O. Garcia Martin and M. Aldegunde. 2009. The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS-222 as anaesthetic agents in the Senegalese sole (Solea senegalensis Kaup, 1858). Aquaculture, 288: 147-150.   DOI
20 Ackerman, P.A., R.B. Forsyth, C.F. Mazur and G.K. Iwama. 2000. Stress hormones and the cellular stress response in salmonids. Fish Physiol. Biochem., 23: 327-336.   DOI
21 Anderson, C.D. and R.J. Roberts. 1975. A comparison of the effects of temperature on wound healing in a tropical and temperate teleost. J. Fish Biol., 7: 173-182.   DOI
22 Chang, Y.J. and J.W. Hur. 1999. Physiological responses of grey mullet (Mugil cephalus) and Nile tilapia (Oreochromis niloticus) by rapid changes in salinity of rearing water. J. Korean Fish. Soc., 32: 310-316. (in Korean)
23 Kim, D.S., I.B. Kim, H.T. Huh and I.-S. Park. 1988. Cytogenetic analysis of catfish (Silurus asotus) (Teleostomi: Siluriformes). Ocean Res., 10: 33-37.
24 Coyle, S.D., S. Dasgupta, J.H. Tidwell and T. Beavers. 2005. Comparative efficacy of anesthetics for the freshwater prawn, Macrobrachium rosenbergii. J. World Aquacult. Soc., 36: 282-290.
25 Gamperl, A.K., M.M. Vijayan and R.G. Boutilier. 1994. Experimental control of stress hormone levels in fishes: techniques and applications. Rev. Fish Biol. Fish., 4: 215-255.   DOI