• 미생물학회지
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• 제19권3호
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• pp.128-136
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• 1981

In order to investigate early identification of species of the wood-decaying fungi in the the mycelial stage, the authors isolated of 41 species, 28 genus, 9 family at 8 locations in Korea and cultivated these isolates on the various kinds of solid media. After investigating such cultural characteristics as oxidase reactions with tannic and gallic acid, various morphological features of colony and growth grade, appeared on the various media, the authors obtained the following results : 1. The oxidase reactions with tannic and gallic acid in the PDTA, DTA, PDGA and DGA media are available for identificantion of the wood-decaying fungi. 2. The oxidase reactions with guaiacol, pyrogallol and hydroquinone in the PDGUA, PDPA and PDHA media are not so much available for identification of the wood-decaying fungi. 3. Morphological features of colonies such as mycelium color, floccose, floccose-powdery, mycelloid, powdery-mycelloid, velvet, radiate, contoured, rosulate and growth grade on the PDA, PSA and PXA media are useful for identification of wood-decaying fungi. 4. It is believed that early identification in species level of wood-decaying fungi using cultural characteristics in the mycelial stage is possible. 5. The key for the identification of 41 species of wood-decaying fungi is proposed by the cultural characteristics using several solid media.

• Journal of the Korean Wood Science and Technology
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• 제48권1호
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• pp.50-60
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• 2020

Fungi are wood-decaying organisms, and this is an important trait that should be considered in wood utilization. When fungi attack wood, it decreases the quality of the wood. The use of metal screws has become an important part of woodworking. The ability of fungi to decay wood and damage metal screws that are embedded into wood is varied. In this study, eight fungal species were evaluated with respect to their ability to decay Parashorea smythiesii and P. tomentella wood. In addition, the effect of fungi on corroding metal screws was determined using the Kolle flask method. The evaluation showed that the fungal species Schizophyllum commune, Pycnoporus sanguineus, and Polyporus arcularius were highly capable of decaying Parashorea spp. woods. The greatest wood weight loss occurred with the heartwood of P. tomentella exposed to S. commune. Based on the classification of wood resistance against fungal attack, the two Parashorea spp. were classified as moderately resistant woods (class III). Schizophyllum commune was classified as highly capable of decaying wood that was embedded with metal screws and was highly capable of corroding metal screws placed in fungi-culture media. The greatest weight of rust powder formed because of screw corrosion was obtained from screw-embedded wood exposed to S. commune. Additionally, the most severe corrosion of metal screws that were embedded into woods was caused by the activities of P. arcularius. Moreover, the average weight loss of screw-embedded wood was greater than that of unscrewed wood.

• Journal of the Korean Wood Science and Technology
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• 제10권3호
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• pp.165-165
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• 1982

In order to improve decaying properties of the plywood, the effectiveness of the Malenit treatment on the shear strength and decaying properties of the plywood has been investigated. The results are as follows. 1. No decrease of the shear strength of the plywood has shown despite of plywood treatment with Malenit right after plywood made or veneer treated with the same preservative prior to plywood manu. factured. 2. The effectiveness of Malenit treatment on decaying property of the plywood has shown much greater than that of the untreated plywood and red pine sapwood. 3. Weight decreases of the test specimens due to the infection of the different fungi have seen in different ways. If fungi infected weight decreases of the specimens are different in it's degree due to the fungi.

• Journal of the Korean Wood Science and Technology
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• 제49권3호
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• pp.234-253
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• 2021

신갈나무는 국내 전역에 두루 분포되어 있는 경제, 산업적으로 활용 가치가 큰 수종이지만, 변색, 부후 등의 열화에 의한 피해가 심각하다. 이러한 이유로 신갈나무의 부후는 목재로써의 활용에 걸림돌이 되나, 부후 요인에 대한 연구는 미비한 실정이다. 본 연구에서는 신갈나무 부후에 영향하는 요인으로 곰팡이에 주목하였으며, 신갈나무의 부후 부위로부터 곰팡이를 분리, 동정하였다. 또한, 동정 된 곰팡이가 실제로 목재 열화에 영향을 미치는지 확인하기 위해 효소 활성을 평가하고, 곰팡이를 처리한 신갈나무 목재의 질량 손실을 목재 부후 실험을 통해 측정하였다. 신갈나무에서 분리된 곰팡이 5종의 genomic DNA의 ITS region을 이용한 염기서열 분석을 통해, Mucoromycota phylum에 속하는 Mucor circinelloides, Cunninghamella elegans, 그리고 Umbelopsis isabellina 3종과 Ascomycota phylum에 속하는 Ophiostoma piceae와 Aureobasidium melanogenum 2종의 곰팡이가 동정되었다. 이러한 5종의 곰팡이는 목재의 부후와 관련된 cellulase나 laccase와 같은 효소 활성이 있으며, 실제로 신갈나무의 심재와 변재의 중량을 감소시켰다. 특히, cellulase와 laccase 활성을 모두 보유한 O. piceae와 A. melanogenum는 신갈나무의 중량을 각각 6.9%와 1.5% 감소시켰다. 이러한 결과들은 본 연구에서 동정된 5종의 곰팡이가 신갈나무의 열화에 영향한다는 것을 의미하며, 신갈나무에 대한 목재 부후균으로써의 가능성을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제10권4호
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• pp.462-469
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• 2000

Sixty-one strains representing the main genera of wood-decaying xylariaceous fungi (mainly in Daldinia, Hypoxylon, Kretzschmaria, Rosellinia, Penzigia, and Xylaria) were tested for their ability to produce ligninolytic enzymes. The phenol oxidase activity and fungal growth of the xylariaceous fungi on gallic aicid and tannic acid media showed a variation in their ability to degrade lignocellulose. A number of species showed equal 개 betterligninolytic enzyme activities than Coriolus versicolor, a known basidiomycete wood-degrader. A large variation of the enzyme activity was observed by individual strains as well as a substantial variation between the isolates of the same species. The most frequent ligninolytic enzymes were peroxidase and general oxidase. With 19% of the strains tested, peroxidase showed the strongest ligninolytic enzyme activity, while tyrosinase activity was detected only in 7% of the strains. All strains of Kretzschmaria and Rosellinia tested was positive for laccase. Xylariaceous fungi were able to degrade the macromolecule, lignin, using each specific ligninolytic enzyme in the specfic lignin degradation pathway.

• 한국산림과학회지
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• 제80권3호
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• pp.311-316
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• 1991

낙엽송 근주심재부후병균(根株心材腐朽病菌)을 이병목(罹病木)의 부후조직(腐朽組織)에서 분리(分離)하였다. 5개도(個道)의 38개임지(個林地)에서 총(總) 216본(本)의 근주심재부후목(根株心材腐朽木)으로부터 가장 높은 빈도(頻度)로 분리(分離)된 균(菌)은 Sparassis crispa(꽃송이버섯)로 조사목(調査木)의 28%를 차지하였고, Laetiporus sulphureus(덕다리버섯)가 19%, Phaeolus schweinitzii(해면버섯)가 8%순(順)으로 분리(分離)되었다. 그러나 조사목(調査木)의 45%에서는 부후균(腐朽菌)이 분리(分離)되지 않았다. 한편 근주심재부후목(根株心材腐朽木)의 수간내(樹幹內)에서 분리(分離)한 4종(種)의 담자균(擔子菌)과 3종(種)의 비담자균(非擔子菌)을 단독(單獨) 또는 상호연계처리(相互連繫處理)하여 낙엽송심재열화능력(心材劣化能力)과 상호관계(相互關係)를 조사(調査)한 결과(結果), 단독처리(單獨處理)에 의(依)한 6개월후(個月後)의 중량감소율(重量減少率)은 P. schweinitzii가 19.6%, S. crispa 10.0%, L. sulphurous 8.2%, Coriolus hirsutus 5.1% 순(順)이었고, 비담자균(非擔子菌)은 4.4%이하(以下)였다. 또한 상호결합처리(相互結合處理)에서 비담자균(非擔子菌)인 Geotrichum candidium, G. sp. 및 Verticillium sp.는 S. crispa, L. sulphurous 및 C. hirsutus의 부후활동(腐朽活動)을 다소(多少) 촉진(促進)시켰으나, P. schweinitziii에는 영향(影響)하지 않았다.

• Journal of the Korean Wood Science and Technology
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• 제24권1호
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• pp.48-53
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• 1996

Brown rot fungus, Tyromyces palustris, has been reported to cause the loss of strength accelerated by oxalate, a non-enzymatic low molecular weight acid, with minute weight loss of decaying wood in early stage. The production of oxalate in relation to wood decaying and the presence of oxaloacetase. an oxalate producing enzyme, were identified during the process. Tyromyces palustris produced the largest amount of oxalate among brown rot fungi. In order to find out the cleavage of pulp fiber, we submerged pulp fiber in oxalate solution and the results showed that the number of short pulp fiber was highly increased, compared with control solution. The pH of decaying wood was decreased to 1.77 which was close to that of saturated oxalate solution, pH 1.2, Thus, the oxalate was thought to be accumulated in the decaying wood, The oxaloacetase which accelerates production of oxalate was derived from fungus, and the production of oxalate by the enzyme was determined by using on UV/Vis spectrophotometer. Therefore, the oxalate was found to be produced by oxaloacetase during decay. The oxalate may cause the acid-hydrolysis of cellulose and hemicellulose. The oxalate was thought to reduce the degree of polymerization and increase the enzyme activity, which resulted in rapid loss of strength in early stage-an identical feature of brown rot fungus.

• 보존과학연구
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• 통권33호
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• pp.99-108
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• 2012

백색부후균(Trametes versicolor)과 갈색부후균(Tyromyces palustris)은 목조문화재 및 건축물 등의 목재 조직의 건조, 부패과 균열, 스펀지화, 변색 등 다양한 손상을 발생시켜 구조적, 미학적인 관점에서 심각한 문제를 나타내고 있다. 그리고 가장 피해를 많이 주는 해충인 흰개미(Reticulitermes speratus Kyushuensis Morimoto)는 목조건축물의 옥외 기둥이나 대들보 등의 표층을 얇게 남겨두고 내부를 침해함으로써, 결국은 속이 빈 기둥이 되어 건축물이 무너져버리는 피해를 줄 수 있다. 대표적 피해 미생물인 목재부후 균이 흰개미의 서식에 미치는 영향을 파악하기 위하여 부후균이 만연된 곳에 흰개미를 넣어 공존 가능성을 실험해 보았다. 목재의 중량으로 피해율을 산정한 결과, 전반적인 총질량은 두 종류의 부후균에서 모두 감소하였다. 목재의 질량은 백색부후균에서는 모두 감소하였으나, 갈색부후균에서는 오히려 증가한 경향을 볼 수 있었다. 그리고 섭식량을 측정하기 위한 목재의 건조 후 질량에서는 갈색부후균에 넣었던 목재의 질량 감소가 더 크게 나타났다.

• 한국균학회지
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• 제47권3호
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• pp.187-197
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• 2019

Ascomycota is the largest phylum of the Fungi, including approximately 6,600 genera. They are often isolated from soils, indoor air, and freshwater environments, but also from plants as pathogens or endophytes. In this study, four species of Ascomycota (two of Cladosporium and one of each Daldinia and Nigrospora) were collected from the leaves of four woody plants (Camellia japonica, Ginkgo biloba, Quercus sp., Vitis vinifera). Their cultural characteristics were investigated on five different media (PDA, V8A, CMA, MEA, CZA) at 3 days after incubation at $25^{\circ}C$ in darkness. BLASTn search and phylogenetic analysis were performed using the internal transcribed spacer (ITS) rDNA sequences, in addition to tef1 gene sequences for Cladosporium species. Based on the cultural, morphological, and phylogenetic data, the isolates were identified as Cladosporium anthropophilum, Cladosporium pseudocladosporioides, Daldinia eschscholtzii, and Nigrospora chinensis. Previously, some members of Cladosporium and Nigrospora have been recorded as endophytes inhabiting the leaves and stems of various plants, whereas Daldinia eschscholtzii is a wood-inhabiting endophyte or wood-decaying fungus. To our knowledge, this is the first report of these four ascomycetes in Korea.

• Mycobiology
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• 제45권1호
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• pp.9-14
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• 2017

Most known species in the Physalacriaceae are saprotrophs that grow on decaying leaves and wood, and approximately 21 genera in the Physalacriaceae have been reported worldwide. During an ongoing survey of indigenous fungi in Korea, four specimens belonging to the Physalacriaceae were collected on Ulleung Island. These specimens were identified as three species based on morphological characteristics and molecular analysis of rDNA-internal transcribed spacer sequences. Three species in three genera were shown to be new records in Korea: Hymenopellis orientalis, Paraxerula hongoi, and Ponticulomyces orientalis. The latter two are the first records of these genera in Korea. In this study, we provide detailed morphological descriptions of these species and describe their phylogenetic position within the Physalacriaceae.