• Korean Journal of Microbiology
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• v.19 no.3
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• pp.128-136
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• 1981

In order to investigate early identification of species of the wood-decaying fungi in the the mycelial stage, the authors isolated of 41 species, 28 genus, 9 family at 8 locations in Korea and cultivated these isolates on the various kinds of solid media. After investigating such cultural characteristics as oxidase reactions with tannic and gallic acid, various morphological features of colony and growth grade, appeared on the various media, the authors obtained the following results : 1. The oxidase reactions with tannic and gallic acid in the PDTA, DTA, PDGA and DGA media are available for identificantion of the wood-decaying fungi. 2. The oxidase reactions with guaiacol, pyrogallol and hydroquinone in the PDGUA, PDPA and PDHA media are not so much available for identification of the wood-decaying fungi. 3. Morphological features of colonies such as mycelium color, floccose, floccose-powdery, mycelloid, powdery-mycelloid, velvet, radiate, contoured, rosulate and growth grade on the PDA, PSA and PXA media are useful for identification of wood-decaying fungi. 4. It is believed that early identification in species level of wood-decaying fungi using cultural characteristics in the mycelial stage is possible. 5. The key for the identification of 41 species of wood-decaying fungi is proposed by the cultural characteristics using several solid media.

• Journal of the Korean Wood Science and Technology
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• v.48 no.1
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• pp.50-60
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• 2020

Fungi are wood-decaying organisms, and this is an important trait that should be considered in wood utilization. When fungi attack wood, it decreases the quality of the wood. The use of metal screws has become an important part of woodworking. The ability of fungi to decay wood and damage metal screws that are embedded into wood is varied. In this study, eight fungal species were evaluated with respect to their ability to decay Parashorea smythiesii and P. tomentella wood. In addition, the effect of fungi on corroding metal screws was determined using the Kolle flask method. The evaluation showed that the fungal species Schizophyllum commune, Pycnoporus sanguineus, and Polyporus arcularius were highly capable of decaying Parashorea spp. woods. The greatest wood weight loss occurred with the heartwood of P. tomentella exposed to S. commune. Based on the classification of wood resistance against fungal attack, the two Parashorea spp. were classified as moderately resistant woods (class III). Schizophyllum commune was classified as highly capable of decaying wood that was embedded with metal screws and was highly capable of corroding metal screws placed in fungi-culture media. The greatest weight of rust powder formed because of screw corrosion was obtained from screw-embedded wood exposed to S. commune. Additionally, the most severe corrosion of metal screws that were embedded into woods was caused by the activities of P. arcularius. Moreover, the average weight loss of screw-embedded wood was greater than that of unscrewed wood.

• Journal of the Korean Wood Science and Technology
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• v.10 no.3
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• pp.165-165
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• 1982

In order to improve decaying properties of the plywood, the effectiveness of the Malenit treatment on the shear strength and decaying properties of the plywood has been investigated. The results are as follows. 1. No decrease of the shear strength of the plywood has shown despite of plywood treatment with Malenit right after plywood made or veneer treated with the same preservative prior to plywood manu. factured. 2. The effectiveness of Malenit treatment on decaying property of the plywood has shown much greater than that of the untreated plywood and red pine sapwood. 3. Weight decreases of the test specimens due to the infection of the different fungi have seen in different ways. If fungi infected weight decreases of the specimens are different in it's degree due to the fungi.

• Journal of the Korean Wood Science and Technology
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• v.49 no.3
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• pp.234-253
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• 2021

The Quercus mongolica has a high utilization value in industrial economic sector. The species is distributed throughout Korea, however, the damage caused by deterioration such as discoloration and decay is severe. For this reason, the deterioration of Q. mongolica is an obstacle to its use as wood, but research on deterioration factors is insufficient. In this study, we focused on fungi as a factor influencing the deterioration of Q. mongolica, and isolated and identified the fungi from the deteriorated Q. mongolica. In additions, in order to confirm whether the identified fungi actually affects wood deterioration, enzyme activities of the identified fungi were evaluated and related mass loss of wood treated with the fungi was measured by wood decay test. As a result of sequencing analysis using the ITS region of the genomic DNA of the fungi isolated from Q. mongolica, Mucor circinelloides, Cunninghamella elegans, and Umbelopsis isabellina 3 species belonging to Mucoromycota phylum, and Ophiostoma piceae and Aureobasidium melanogenum 2 species belonging to Ascomycota phylum were identified. These five fungi had enzyme (i.e. cellulase, laccase) activities related to wood decay and reduced the mass of heartwood and sapwood of Q. mongolica in practice. In particular, O. piceae and A. melanogenum, which have both cellulase and laccase activities, showed 6.9% and 1.5% mass loss, respectively. These results indicated that five fungi identified in this study influence the deterioration of Q. mongolica and are wood decaying fungi for Q. mongolica potentially.

• Journal of Microbiology and Biotechnology
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• v.10 no.4
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• pp.462-469
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• 2000

Sixty-one strains representing the main genera of wood-decaying xylariaceous fungi (mainly in Daldinia, Hypoxylon, Kretzschmaria, Rosellinia, Penzigia, and Xylaria) were tested for their ability to produce ligninolytic enzymes. The phenol oxidase activity and fungal growth of the xylariaceous fungi on gallic aicid and tannic acid media showed a variation in their ability to degrade lignocellulose. A number of species showed equal 개 betterligninolytic enzyme activities than Coriolus versicolor, a known basidiomycete wood-degrader. A large variation of the enzyme activity was observed by individual strains as well as a substantial variation between the isolates of the same species. The most frequent ligninolytic enzymes were peroxidase and general oxidase. With 19% of the strains tested, peroxidase showed the strongest ligninolytic enzyme activity, while tyrosinase activity was detected only in 7% of the strains. All strains of Kretzschmaria and Rosellinia tested was positive for laccase. Xylariaceous fungi were able to degrade the macromolecule, lignin, using each specific ligninolytic enzyme in the specfic lignin degradation pathway.

• Journal of Korean Society of Forest Science
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• v.80 no.3
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• pp.311-316
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• 1991

Butt-rot fungi were isolated from decayed portion in butt rotted larch trees[Larix leptolepis(Sieb. et Zucc.) Gord.]. From a total of 216 affected trees in 38 forests of five provinces, Sparassis crispa(Wulf. ex Fr.) Fr. was isolated with the highest frequency, i.e.. 28% of the total trees. Laetiporus sulphureus(Fr.) Bond. et Sing, with 19%, and Phaeolus schweinitzii(Fr.) Pat. with 8%. However, none of these fungi was isolated from 45% of the trees. Wood decaying abilities and interaction of four basidiomycetous and three non-basidiomyo:etous fungi isolated from butt rotted trees were investigated. The weight loss of larch wood blocks, when exposed to the culture of a fungus for six months, was 19.6% for P. schweinitzii, 10% for S. crispa, 8.2% for L. sulphurous, 5.1% for Coriolus hirsutus, and less than 4.4% for three non-basidiomycetous fungi. In incubation with combinations of the fungi, the wood decay activity of S. crispa, L. sulphurous and C. hirsutus was increased by the non-basidiomycetous fungi of Geotrichum candidacy, G. sp. and Verticillium sp., but that of P. schweinitzii was not.

• Journal of the Korean Wood Science and Technology
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• v.24 no.1
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• pp.48-53
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• 1996

Brown rot fungus, Tyromyces palustris, has been reported to cause the loss of strength accelerated by oxalate, a non-enzymatic low molecular weight acid, with minute weight loss of decaying wood in early stage. The production of oxalate in relation to wood decaying and the presence of oxaloacetase. an oxalate producing enzyme, were identified during the process. Tyromyces palustris produced the largest amount of oxalate among brown rot fungi. In order to find out the cleavage of pulp fiber, we submerged pulp fiber in oxalate solution and the results showed that the number of short pulp fiber was highly increased, compared with control solution. The pH of decaying wood was decreased to 1.77 which was close to that of saturated oxalate solution, pH 1.2, Thus, the oxalate was thought to be accumulated in the decaying wood, The oxaloacetase which accelerates production of oxalate was derived from fungus, and the production of oxalate by the enzyme was determined by using on UV/Vis spectrophotometer. Therefore, the oxalate was found to be produced by oxaloacetase during decay. The oxalate may cause the acid-hydrolysis of cellulose and hemicellulose. The oxalate was thought to reduce the degree of polymerization and increase the enzyme activity, which resulted in rapid loss of strength in early stage-an identical feature of brown rot fungus.

• 보존과학연구
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• s.33
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• pp.99-108
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• 2012

The white-rot fungus (Trametes versicolor) and brown-rot fungus (Tyromyces palustris), which cause damage to a variety of wooden cultural properties and buildings, such as drying of the wood tissue, decay and cracks, sponge, and discoloration, give rise to serious structural and aesthetic problems. Moreover, pest termites (Reticulitermes speratus kyushuensis Morimoto) can cause damages like hollowing the outdoor beams or pillars of wooden buildings and finally causing such buildings to collapse due to the termites' destruction of the inside of the beams or pillars, leaving only a thin layer on their surfaces. This study was conducted to determine the impact of the wood-decay fungus, a representative damage-causing microorganism, and of insects and termites on the termite ecology. The damage ratio was calculated as the weight of the timber, and the overall total mass was reduced from two kinds of rot fungi. The white-rot fungi reduced the total wood mass, but the brown-rot fungi were observed to have had an increasing tendency to do so. The wood mass was measured after drying to determine the destruction capacity of termites. As a result, the wood mass consumed by the brown-rot fungi was shown to be greater.

• The Korean Journal of Mycology
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• v.47 no.3
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• pp.187-197
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• 2019

Ascomycota is the largest phylum of the Fungi, including approximately 6,600 genera. They are often isolated from soils, indoor air, and freshwater environments, but also from plants as pathogens or endophytes. In this study, four species of Ascomycota (two of Cladosporium and one of each Daldinia and Nigrospora) were collected from the leaves of four woody plants (Camellia japonica, Ginkgo biloba, Quercus sp., Vitis vinifera). Their cultural characteristics were investigated on five different media (PDA, V8A, CMA, MEA, CZA) at 3 days after incubation at $25^{\circ}C$ in darkness. BLASTn search and phylogenetic analysis were performed using the internal transcribed spacer (ITS) rDNA sequences, in addition to tef1 gene sequences for Cladosporium species. Based on the cultural, morphological, and phylogenetic data, the isolates were identified as Cladosporium anthropophilum, Cladosporium pseudocladosporioides, Daldinia eschscholtzii, and Nigrospora chinensis. Previously, some members of Cladosporium and Nigrospora have been recorded as endophytes inhabiting the leaves and stems of various plants, whereas Daldinia eschscholtzii is a wood-inhabiting endophyte or wood-decaying fungus. To our knowledge, this is the first report of these four ascomycetes in Korea.

• Mycobiology
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• v.45 no.1
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• pp.9-14
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• 2017

Most known species in the Physalacriaceae are saprotrophs that grow on decaying leaves and wood, and approximately 21 genera in the Physalacriaceae have been reported worldwide. During an ongoing survey of indigenous fungi in Korea, four specimens belonging to the Physalacriaceae were collected on Ulleung Island. These specimens were identified as three species based on morphological characteristics and molecular analysis of rDNA-internal transcribed spacer sequences. Three species in three genera were shown to be new records in Korea: Hymenopellis orientalis, Paraxerula hongoi, and Ponticulomyces orientalis. The latter two are the first records of these genera in Korea. In this study, we provide detailed morphological descriptions of these species and describe their phylogenetic position within the Physalacriaceae.