• 제목/요약/키워드: wood industry

검색결과 603건 처리시간 0.023초

Transformation of Terpene Synthase from Polyporus brumalis in Pichia pastoris for Recombinant Enzyme Production

  • An, Ji-Eun;Lee, Su-Yeon;Ryu, Sun-Hwa;Kim, Myungkil
    • Journal of the Korean Wood Science and Technology
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    • 제46권4호
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    • pp.415-422
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    • 2018
  • Terpenoids have a wide range of biological functions and have extensive applications in the pharmaceutical, cosmetic, and flavoring industry. The white-rot fungus, Polyporus brumalis, is able to synthesize terpenoids via terpene synthase, which catalyzes an important step that forms a large variety of sesquiterpene products from farnesyl pyrophosphate (FPP). To improve the production of sesquiterpenes, the terpene synthase gene was isolated from Polyporus brumalis and was heterologously transformed into a Pichia pastoris strain. The open reading frame of the isolated gene (approximately 1.2 kb) was inserted into Pichia pastoris to obtain a recombinant enzyme. Five transformants were obtained and the expression of terpene synthase was analyzed at the transcript level by reverse transcription PCR (polymerase chain reaction) and at the protein level by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Expression of the terpene synthase gene product was elevated in the transformants and as expected the molecular weight of the protein was approximately 45 kDa. These recombinant enzymes have potential practical applications and future studies should focus on their functional characterization.

감즙 염색 한지의 특성 (Properties of Hanji Dyed with the Persimmon Juice)

  • 유승일;이상현;곽미례;최태호
    • 펄프종이기술
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    • 제42권2호
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    • pp.82-87
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    • 2010
  • The Hanji dyed with persimmon juice were useful goods in the past, but they are not widely used nowadays despite of their many strong points such as antibacterial and water-resistant properties. The object of this study is to provide useful data for developing new products by using them. We dyed traditional Korean hand-made papers (Hanji) with colorants extracted from fruits of Diospyros kaki and investigated the effect of various dyeing factors (mordant, mordanting method, pH of dyeing solution, light exposure time etc.) on optical and mechanical properties of the dyed Hanji. Changing mordant affected the color of dyed Hanji. The dyed Hanji after-mordanted with Cu mordant had the highest K/S value and showed the deepest yellow and red shade. The best mordanting method was an after-mordanting. The K/S value of dyed Hanji increased with decreasing pH of dyeing solution. The tensile index and size degree values of the Hanji were increased after dyeing.

아프리카 튤립으로 제조한 soda 및 soda-AQ 펄프의 특성 (Soda and Soda-AQ Pulps Properties from African Tulip Tree)

  • 이재성;송우용;신수정
    • 펄프종이기술
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    • 제47권5호
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    • pp.85-90
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    • 2015
  • To use the African tulip tree (Spathodea campanulata) as raw material for chemical pulping, soda and soda-AQ pulping was investigated. In chemical compositional analysis, lignin contents of African tulip (33.1%) was higher than other hardwood such as Yellow poplar (17.5%), Acacia (27.1%), or Eucalyptus (24.2%). Soda or soda-AQ pulping with African tulip tree resulted in yield at 43.4-44.8% with 29.5-34.5 Kappa number, pulping condition with 20-22% active alkali. Kappa number of African tulip tree pulp was quite higher than other tropical hardwood (Eucalyptus or Accacia) with less yield due to higher lignin content in wood. Fiber length and width of pulp from African tulip tree was similar to Accacia pulp and shorter than eucalyptus pulp.

진한 황산 가수분해 반응조건에서 xylose와 glucuronic acid의 반응 특성 (Characteristics of xylose and glucuronic acid at concentrated sulfuric acid hydrolysis)

  • 조대행;김용환;박종문;심재훈;김병로;신수정
    • 펄프종이기술
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    • 제44권3호
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    • pp.9-14
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    • 2012
  • Formed fermentation inhibitors during acid saccharification leads to poor alcohol production based on lignocellulosic bio-alcohol production process. In this work, it is focused on the formation of fermentation inhibitors from xylan, which is influenced by reaction tempearature and time of acidic sacharifiaction of xylose and glucuronic acid. In second step of concentrated acid hydrolysis, part of xylose and glucuronic acid was converted to furfuraldehyde and formic acid by dehydration and rearrangement reactions. Furfural was form from xylose, which was highly sensitive to reaction temperature. Formic acid was come from both xylose and glucuronic acid, which supposed to main inhibitor in biobutanol fermentation. Reaction temperature of second hydrolysis was main variables to control the furfural and formic acid generation. Careful control of acid saccharification can reduce generation of harmful inhibitors, especially second step of concentrated sulfuric acid hydrolysis process.

양이온화 처리 한지의 천연염색성 (Improvement on Dyeability of Hanji with Natural Dyes Using a (3-chloro-2-hydroxypropyl) Trimethyl Ammonium Chloride)

  • 유승일;오의명;민유리;최태호
    • 펄프종이기술
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    • 제43권3호
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    • pp.88-97
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    • 2011
  • We carried out cationization of Dak pulp (paper mulberry bast fiber pulp) which is raw material of Hanji (Traditional Korean Paper) using a (3-chloro-2-hydroxypropyl) trimethyl ammonium chloride to improve dyeability during a dyeing of Hanji with Gardenia (Gardenia jasminoides) and smoke tree (Cotinus coggygria). Fiber specific charge densities were determined using polyelectrolyte titration method and K/S values of dyed Hanji was calculated by Kubelka-Munk equation. As the result, fiber specific charge density increased with degree of cationization. Colors of Hanji dyed with Gardenia did not vary significantly with degree of cationization, but cationized Hanji dyed with smoke tree showed a large increase of a* value and reddish yellow color. After-mordanting did not decrease K/S value of dyes with cationized Hanji. K/S values of dyed Hanji decreased with increasing dyeing temperature. For smoke tree, the cationization impair lightfastness of dyed Hanji without mordant. After-mordanting with copper acetate or iron chloride improved lightfastness of dyed Hanji.

목재섬유와 면섬유의 혼합에 따른 수초지의 파괴인성 변화 (Changes of Handsheet Fracture Toughness by Wood and Cotton Fibers Mixing)

  • 김정중;장동욱;윤상구;신현식;박종문
    • 펄프종이기술
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    • 제46권3호
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    • pp.81-87
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    • 2014
  • Conditions of paper manufacturing process should be changed depending on the end use and paper properties. Most of the case, mixed pulps with long softwood fibers and short hardwood fibers are used to achieve proper qualities of product with reasonable production cost. For specialty paper manufacture the wood pulp and cotton linter pulp are usually mixed together. The objectives of this study is to analyze physical, mechanical and fracture mechanical properties of paper depending on SwBKP, HwBKP and cotton linter pulp(CLP) mixing. When the mixing ratio of SwBKP was increased, strength properties, such as tensile, tear, and folding endurance, were also increased. When the mixing ratio of SwBKP and HwBKP was increased, stress concentration index was decreased and fracture toughness was increased.

Enzyme beating 전처리를 통한 Micro-Fibrillated Cellulose 제조 및 지력증강 효과 (Micro-Fibrillated Cellulose Preparation with Enzyme Beating Pretreatment and Effect on Paper Strength Improvement)

  • 안은별;홍성범;김강재;엄태진
    • 펄프종이기술
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    • 제47권6호
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    • pp.57-65
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    • 2015
  • Microfibrillated cellulose (MFC) or Nanofibrillated cellulose (NFC) has been used to reduce the use of raw pulp and to improve paper strength. The problem of MFC preparation is high manufacturing cost. In this study, it was carried out to prepare MFC after enzyme beating and estimated properties of MFC. Endo-D was the best beating efficiency among three type of endo-glucanase. As the grinder pass number increased, the viscosity and the fines of MFC suspension increased while the crystallinity and the porosity of MFC sheet decreased. Also enzyme beating MFC was higher value in the crystallinity and lower value in the viscosity than non-enzyme MFC. In addition, the aspect ratio of MFC was the highest at 5 pass. MFC addition improved the handsheet strength and the air permeability but worsened the drainage.

제지공정의 유기 충전제로서 침엽수 잎 분말 적용 (Application of Conifer Leave Powder to the Papermaking Process as an Organic Filler)

  • 성용주;김동성;이지영;서영범;임창국;권완오;김진두
    • 펄프종이기술
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    • 제46권4호
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    • pp.62-68
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    • 2014
  • The application of conifer leave to the papermaking process as a functional organic filler was investigated in this study. The powder of the conifer leave after hot water extraction for the functional extract, such as phytoncide, was applied to OCC stock. The comparison between the commercial wood flour and the conifer leave powder as organic filler for OCC paper were conducted with various wet pressing conditions. The amount of the water removal by the wet pressing process and the bulk of handsheet were increased by the addition of the wood flour and the conifer leave powder, although the tensile strength was decreased. At the higher pressure condition of the wet pressing, the wet pressing efficiency was greatly increased by the wood flour and the conifer leave powder. There was a little difference in the performance of the wood flour and the conifer leave powder as an organic filler. Those results showed the conifer leave powder could be an alternative resource to the wood powder for papermaking organic filler.

비목재펄프를 이용한 도공원지의 특성이 도공지의 물성 및 인쇄적성에 미치는 영향(II) -볏짚 표백 크라프트펄프- (Effects of Base Papers Containing Nonwood Pulp on the Properties and Printabilities of Coated Papers(II) -Rice Straw Bleached Kraft Pulp-)

  • 임현아;강진하;이용규
    • 펄프종이기술
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    • 제32권4호
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    • pp.49-57
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    • 2000
  • This study was carried out to investigate the effect of the type of base papers containing rice straw-BKP on the properties and printabilities of coated papers. Also, it was intended to evaluate the effect of coated paper prepared with anionic and amphoteric latex based coating color. The results obtained from this study were as follows. The fiber length of rice straw-BKP was observed much shorter than those of the wood pulps(Sw-BKP, Hw-BKP). This has effect on physical properties. Therefore, the results of measured physical properties were much lower than those of wood pulps. Also, physical properties tended to increase slightly with the increase of the mixing ratio of Sw-BKP. Opacity of the base paper containing rice straw-BKP, was higher than that of the wood pulps. As the mixing ratio of Sw-BKP increases the opacity tended to decrease. On the other hand, smoothness and roughness to rice straw-BKP were similar with Sw-BKP and its air permeability was lower than those of wood pulps. The optical properties of coated papers containing rice straw-BKP tended to be similar to those of other base papers. The ink receptivity of coated papers containg rice straw-BKP was higher than those of wood pulps and printing gloss was similar to wood pulps. The mixing ratio of Sw-BKP, the ink receptivity and printing gloss of rice straw-BKP were showed no difference irrespective the mixing ratio of Sw-BKP. Meannwhile, amphoteric latex improved the optical properties and printability of coated papers.

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목제품 시장에서 국산품과 수입품의 아밍턴 대체 탄성치 추정 (Estimating Armington Substitution Elasticity between Domestic and Imported Wood Products in Korea)

  • 민경택
    • 한국산림과학회지
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    • 제104권2호
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    • pp.254-260
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    • 2015
  • 이 연구는 수입 목제품과 국산 목제품의 수요가 불완전 대체관계에 있다고 가정하고 아밍턴 대체 탄성치를 추정하였으며 이에 근거하여 수입 목제품의 관세 인하가 국내 목제품 생산에 미치는 영향을 분석한 것이다. 분석 대상 목제품은 합판, 섬유판, 파티클보드로 하였다. 분석결과, 국산 목제품과 수입 목제품은 완전 대체관계에 있지는 않은 것으로 나타났다. 합판과 섬유판에서 국산품과 수입품의 대체 탄성치가 높게 나타나 관세를 인하하였을 때 그 영향이 적지 않을 것으로 생각된다. 이에 비해 파티클보드 시장에서 국산품과 수입품은 차별화되어 있으며 대체 관계가 낮은 것으로 보인다. 섬유판은 국내 숲가꾸기 산물을 원료로 사용하고 있기 때문에 섬유판 생산의 위축은 국내 임업에도 부정적 영향을 미치게 될 것이다. 따라서, 시장개방에 대응하여 국내 목제품 산업의 경쟁력 강화를 위한 대책이 필요하다.