• Proceedings of the PSK Conference
• /
• 2002.10a
• /
• pp.303.2-304
• /
• 2002

Wogonin. a flavonoid originated from the root of Scutellaria baicalensis Georgi. is known to exhibit potent anti-inflammatory effects and variable degrees of antioxidant and free radical scavenging effects depending on the experimental systems. In addition. wogonin has been reported to protect neurons from excitotoxic and oxidative injuries in primary cultured rat cortical cells. (omitted)

• The Korea Journal of Herbology
• /
• v.22 no.2
• /
• pp.189-199
• /
• 2007

Objective : This study was observed the variation of components from Scutellariae Radix (SR) by processing and storage. Methods : The main component of SR is analysed after various processing and also storage condition by HPLC. Result : 1. In wine-fried SR, the contents of baicalein, wogonin and oroxylin-A was significantly increased. However, the content of baicalin was decreased slightly. In charred SR, the contents of baicalein, wogonin and oroxylin- A was significantly increased. However, the content of baicalin was decreased greatly. 2. The contents variation of SR was not almost changed in room and cold condition. But in accelerated condition, the content of baicalin was slightly decreased, and the contents of baicalein, wogonin and oroxylin-A was significantly increased. 3. The contents variation of wine-fried SR was not almost changed in room and cold condition. But in accelerated condition, the content of baicalin was signifjcantly decreased. 4. The contents variation of charred SR was not almost changed in all conditions. Conclusion : The bioactivity of SR and wine fried SR might be changed by the contents varition during longterm storage. charred SR can be preserved more than two years in room and cold condition.

• Bulletin of the Korean Chemical Society
• /
• v.33 no.1
• /
• pp.177-182
• /
• 2012

A new composition of standardized Scutellariae Radix extract (HPO12) was developed for treatment of Alzheimer's disease. For the preclinical pharmacokinetic study of HPO12, a rapid, sensitive, and selective LCMS/MS method was developed and validated for the simultaneous determination of 4 bioactive compounds, baicalein, baicalin, wogonin, and wogonoside. After extraction with ethylacetate, chromatographic analysis was performed on a Thermo $C_{18}$ column ($150mm{\times}2.1mm$, $3{\mu}m$) with a mobile phase consisting of 0.1% formic acid (A) and 0.1% formic acid in 95% acetonitrile (B) by using gradient elution at a flow rate of $250{\mu}L/min$. Analytes introduced to a mass spectrometer were monitored by multiple reaction monitoring (MRM) in positive ion mode. Using $25{\mu}L$ of plasma sample, the method was validated over the following concentration ranges: 25-5000 ng/mL for baicalein, 20-40000 ng/mL for baicalin, 1-1000 ng/mL for wogonin, and 5-10000 ng/mL for wogonoside. The intra- and inter-day precision and accuracy of the quality control samples at the 4 concentrations showed $\leq$ 13.7% relative standard deviation (RSD) and 86.6-105.5% accuracy. The method was successfully applied to determine the concentrations of baicalein, baicalin, wogonin, and wogonoside in rat plasma after intraperitoneal and oral administrations of HPO12.

• Biomolecules & Therapeutics
• /
• v.24 no.3
• /
• pp.328-337
• /
• 2016

We examined whether wogonin (WO) improved hippocampal neuronal activity, behavioral alterations and cognitive impairment, in rats induced by administration of trimethyltin (TMT), an organotin compound that is neurotoxic to these animals. The ability of WO to improve cognitive efficacy in the TMT-induced neurodegenerative rats was investigated using a passive avoidance test, and the Morris water maze test, and using immunohistochemistry to detect components of the acetylcholinergic system, brain-derived neurotrophic factor (BDNF), and cAMP-response element-binding protein (CREB) expression. Rats injected with TMT showed impairments in learning and memory and daily administration of WO improved memory function, and reduced aggressive behavior. Administration of WO significantly alleviated the TMT-induced loss of cholinergic immunoreactivity and restored the hippocampal expression levels of BDNF and CREB proteins and their encoding mRNAs to normal levels. These findings suggest that WO might be useful as a new therapy for treatment of various neurodegenerative diseases.

• Natural Product Sciences
• /
• v.23 no.1
• /
• pp.46-52
• /
• 2017

The aim of this study was to evaluate the anti-Helicobacter pylori activity of fractions and major aglycon compounds (baicalein, chrysin, oroxylin A, wogonin) of Scutellariae Radix. Minimum inhibitory concentration (MIC) measurement, DPPH radical-scavenging assay, DNA protection assay, and urease inhibition analysis were performed. The ethyl acetate (EtOAc) fraction showed the potent anti-Helicobacter activity, and therefore, compounds in the EtOAc fraction were subjected to further assay. The MICs of chrysin, oroxylin A, and wogonin against Helicobacter pylori 26695 were 6.25, 12.5 and $25{\mu}g/mL$, respectively. Baicalein exhibited the most effective DPPH radical-scavenging activity. DNA protection using Fenton reaction, chrysin, oroxylin A, and wogonin showed effective DNA protective effect. This result was also confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Regarding Jack bean urease (0.5 mg/mL, 50 unit/mg) inhibition, 20 mM ofbaicalein and chrysin inhibited urease activity by 88.2% and 72.5%, respectively.

• Proceedings of the PSK Conference
• /
• 2002.10a
• /
• pp.302.2-303
• /
• 2002

Oxidative stress has been implicated in the pathophysiology of many neurodegenerative disorders including Alzheimer's and Parkinson's diseases. Baicalein. baicalin and wogonin. the major constituents of Scutellaria baicalensis. have been reported to exhibit antioxidant properties in many different bioassay systems. The present study evaluated neuroprotective effects of these flavonoids on various neuronal injuries induced in primary cultured rat cortical cells by oxidative stress. NMDA. oxygen-glucose deprivation. and $A{\beta}$(25-35). (omitted)

• YAKHAK HOEJI
• /
• v.52 no.1
• /
• pp.85-91
• /
• 2008

8-Acyl and 8-sulfonylamidowogonin analogues were synthesized as potential anti-inflammatory agents. Nitration of 5,7-dihydroxyflavone (chrysin) followed by methylation of phenol groups and reduction of nitro group yielded 8-aminowogonin analogues. Acylation and sufonylation of 8-aminowogonin followed by demethylation reactions gave the title compunds. The synthesized wogonin analogues showed much reduced inhibitory activity on prostaglandin $E_2\;(PGE_2)$ production.

• Proceedings of the Korea Environmental Mutagen Society Conference
• /
• 2004.11a
• /
• pp.128-128
• /
• 2004

• Korean Journal of Pharmacognosy
• /
• v.31 no.2
• /
• pp.157-162
• /
• 2000

The herb of Melampyrum roseum var. hirsutum Beauv. was extracted with methanol and the extract was fractionated with n-hexane, chloroform, ethylacetate and n-buthanol. Repeated column chromatography of silica gel, Sephadex LH-20 and Lobar-A lichroprep Si 60 led to the isolation of five compounds from n-hexane layer and ethylacetate layer. These compounds were identified as apigenin, linarin, pectolinarin, wogonin and mussaenoside, respectively, by the physicochemical and spectral properties.

• Archives of Pharmacal Research
• /
• v.22 no.1
• /
• pp.18-24
• /
• 1999

Various flavonoid derivatives were previously reported to possess the inhibitory activity on cyclooxygenase/lipoxygenase. And these properties of flavonoids might contribute to their anti-inflammatory activity in vivo. In this study, several polyhydroxylated/methoxylated flavonoid derivatives such as oroxylin A. wogonin, skullcapflavone II, tectorigenin and iristectorigenin A were isolated from the medicinal plants. these compounds were evaluated fro their inhibitory effects on cyclooxygenase/lipoxygenase from the homogenate of human platelets in vitro. It was found that isoflavones including daidzein and tectorigenin possessed the inhibitory activity on cycloooxygenase, although the potency of inhibition was far less than that of indomethacin. In addition, oroxylin A, baicalein and wogonin inhibited 12-lipoxygenase activity without affecting cyclooxygenase, which suggested that 5,6,7- or 5,7,8-trisubstitutions of A-ring of flavone gave favorable results. The IC50 values of oroxylin A and NDGA against 12-lipoxygenase were found to be 100 and 1.5 uM, respectively.