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http://dx.doi.org/10.20307/nps.2017.23.1.46

Comprehensive Evaluation of the Anti-Helicobacter pylori Activity of Scutellariae Radix  

Lee, Ba Wool (College of Pharmacy, Sahmyook University)
Park, Il-Ho (College of Pharmacy, Sahmyook University)
Yim, Dongsool (College of Pharmacy, Sahmyook University)
Choi, Sung Sook (College of Pharmacy, Sahmyook University)
Publication Information
Natural Product Sciences / v.23, no.1, 2017 , pp. 46-52 More about this Journal
Abstract
The aim of this study was to evaluate the anti-Helicobacter pylori activity of fractions and major aglycon compounds (baicalein, chrysin, oroxylin A, wogonin) of Scutellariae Radix. Minimum inhibitory concentration (MIC) measurement, DPPH radical-scavenging assay, DNA protection assay, and urease inhibition analysis were performed. The ethyl acetate (EtOAc) fraction showed the potent anti-Helicobacter activity, and therefore, compounds in the EtOAc fraction were subjected to further assay. The MICs of chrysin, oroxylin A, and wogonin against Helicobacter pylori 26695 were 6.25, 12.5 and $25{\mu}g/mL$, respectively. Baicalein exhibited the most effective DPPH radical-scavenging activity. DNA protection using Fenton reaction, chrysin, oroxylin A, and wogonin showed effective DNA protective effect. This result was also confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Regarding Jack bean urease (0.5 mg/mL, 50 unit/mg) inhibition, 20 mM ofbaicalein and chrysin inhibited urease activity by 88.2% and 72.5%, respectively.
Keywords
Scutellariae Radix; Helicobacter pylori; MIC; DNA protection assay; Urease inhibitory activity;
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