• BMB Reports
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• 제39권6호
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• pp.749-758
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• 2006

The cytosolic members of the HSP70 family of proteins play key roles in the molecular chaperone machinery of the cell. In the study we cloned and sequenced the full-length cDNA of Delia antiqua HSP70 gene, which is 2461 bp long and encodes 643 a.a. with a calculated molecular mass of 70,787 Da. We investigated gene copies of cytosolic HSP70 members of 4 insect species with complete genome available, and found that they are quite variable with species. In order to characterize this protein we carried out an alignment and a phylogenetic analysis with 41 complete protein sequences from insects. The analysis divided the cytosolic members of the family into two classes, HSP70 and HSC70, distinguishable on the basis of 15 residues. HSP70 class members were slightly shorter in length and smaller in molecular mass relative to the HSC70 class members, and the conservative and functional regions in these sequences were documented. Mainly, we investigated the expression of Delia antiqua HSP70 gene, in response to diapauses and thermal stresses. Both summer and winter diapauses elevated HSP70 transcript levels. Cold-stress led to increased HSP70 expression levels in summer- and winter-diapausing pupae, but heat-stress elevated the levels only in the winter-diapausing pupae. In all cases, the expression levels, after being elevated, gradually decreased with time. HSP70 expression was low in non-diapausing pupae but was up-regulated following cold- and heat-stresses. Heat-stress gradually increased the mRNA level with time whereas cold-stress gradually decreased levels after an initial increase.

• Asian-Australasian Journal of Animal Sciences
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• 제24권4호
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• pp.509-516
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• 2011

The performance of naked neck and normal chicken was evaluated with respect to growth, carcass, immune, biochemical and stress parameters under winter and summer seasons to assess the suitability of naked neck birds under high temperatures in the global scenario of climate change. The growth performance was significantly ($p{\leq}0.05$) higher in naked neck chicken in the summer season. The dressing percentage was significantly ($p{\leq}0.05$) higher in naked neck birds in both winter and summer season because of reduced plumage. The thigh, giblet and feather proportion significantly ($p{\leq}0.05$) varied between naked neck and normal chickens in summer season. The humeral immune response to sheep red blood cells (SRBC), Newcastle disease vaccine (NDV) and cutaneous basophil hypersensitivity (CBH) did not show any significant differences among the chicken groups. The protein and cholesterol concentration observed was within the normal ranges. The total cholesterol levels in plasma were significantly ($p{\leq}0.05$) lower in naked neck birds in both the seasons. H:L ratio was significantly ($p{\leq}0.05$) lower in summer season indicating less stress in naked neck chicken. Basophil and eosinophil concentration was significantly ($p{\leq}0.05$) higher in normal chicken in summer. The lipid peroxidation was higher in full feathered birds under summer stress. The enzyme glutathione reductase (GR) levels were significantly higher during the summer and varied significantly ($p{\leq}0.05$) between the normal and naked neck chicken in both seasons. The results indicated that the naked neck birds performed significantly better at high ambient temperatures with respect to growth, carcass and biochemical parameters. It was concluded that the ability of the naked neck chicken to adapt to high temperatures foresees a viable option for the biological mitigation of climate change.

• Journal of Animal Science and Technology
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• 제61권6호
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• pp.340-351
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• 2019

Hot environments can affect feed intake and lactation, and the subsequent unavailability of important micronutrients to the newborn piglet can impair piglet growth, reduce the viability of newborn piglets and limit their subsequent performance. This work addresses the effects of hot environments (summer season) upon the reproductive performance of sows during gestation and lactation as well as on the serum levels of vitamins and the concentration of immunoglobulins in their litters in comparison with the winter season. Fourteen sows were evaluated over 100 ± 2 days of gestation in each season. The temperature and humidity index (THI) was used as an indirect measure of heat stress during gestation. The reproductive performance, milk yield, and body condition of the sows were recorded. The concentrations of vitamin E and vitamin A in piglets and in sow serum, colostrum, milk and feed were determined by HPLC; immunoglobulins were assessed by an ELISA. The THI index indicated that animals were subject to heat stress only in during the summer. Although the effect was not significant, there were a lower number of piglets at birth and at weaning and the milk yield in summer compared with winter. There was no difference (p > 0.05) in the body condition of sows between seasons. Season had an effect (p < 0.05) on the vitamin A concentration of postpartum sow serum (0.29 ㎍/mL in winter vs 0.21 ㎍/mL in summer) and on the vitamin E concentration before birth (2.00 ㎍/mL in winter vs 0.90 ㎍/mL in summer). Vitamin E in milk was higher (p < 0.05) in winter than in summer (2.23 vs 1.81 ㎍/mL). Serum levels of vitamins A and E in piglets at birth were lower (p < 0.05) in winter than in summer. The concentrations of immunoglobulins (IgG and IgA) in colostrum and milk were similar between seasons (p > 0.05), but the IgA in piglet serum was higher in winter than in summer (p < 0.05). High temperatures produced heat stress in sows, which affected certain aspects of production that can be translated into economic losses for this sector.

• 한국수정란이식학회지
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• 제23권4호
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• pp.257-261
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• 2008

The objective of present study was to investigate the effect of seasons on reproductive performance of Hanwoo and Holstein heifers. Heat stress in summer or cold stress in winter stress to Hanwoo and Holstein heifers may bring reproduction failure, which would pose an important economic loss, even around Daegwallyeong region located in high mountainous area. Seasonal differences in the serum levels of LH, FSH and progesterone ($P_4$) in response to environmental factors (hot and cold) out of 20 pubertal Hanwoo heifers in Daegwallyeong, Gangwon Province and 20 non-lactating Holstein heifers in Chonan city of Republic of Korea at 2-3 years of age were compared. Blood samples for hormonal analysis were from jugular vein after detection of estrus repeatedly over four seasons within four-week intervals (Spring: May to June, Summer: July to August, Autumn: October to November and Winter: January to February). In Hanwoo heifer population, averages of LH and FSH concentration in spring and in summer were greater compared to those in winter (p<0.05). LH or FSH levels tended to be greater (p=0.06) in spring and less (p=0.09) in winter compared to the levels in autumn. Only in summer, cattle seemed to show lower LH or FSH secretion (p<0.05). Similar to the results in Hanwoo heifers, the serum concentrations of LH and FSH in Holstein heifers decreased further by heat stress in summer when P 4 levels were high during luteal phase. The results demonstrate significant effect of summer heat on reproduction of Hanwoo or Holstein heifers. Although parameters indicating the extent of heat stress were not measured in this study, we suggest that serum hormone levels could be considered as successful indicators of summer heat stress condition for Hanwoo and Holstein heifers even under rather cool summer climate.

• 대한지리학회지
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• 제39권1호
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• pp.13-26
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• 2004

본 연구는 상대적 날씨 스트레스 지수로 NET의 적용 가능성을 파악하고자 하였다. 기상청에서 예보하고 있는 기온, 습도, 바람 자료를 이용하여 NET 값의 시공간적 분포 특성을 분석하였다. 여름철 스트레스 지수인 일 최고 NET의 지역별 스트레스 기준값은 바람과 습도보다는 기온의 영향을 받으므로 일 최고 기온의 분포와 유사하다. 겨울철 스트레스 지수인 일 최저 NET의 스트레스 기준값은 산지 지역과 서울 이북 지역에서는 낮은 기온의 영향을. 해안 지역에서는 강한 바람의 영향으로 기준값이 여름철에 비하여 다양하게 나타난다. 스트레스가 강한 날의 발생 빈도는 여름철은 뚜렷한 연변화가 나타나지 않지만, 겨울철에는 1990년대 중반 이후 뚜렷한 증가 경향을 보인다.

• Ocean and Polar Research
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• 제34권4호
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• pp.395-401
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• 2012

Ten-year AVHRR sea surface temperature data obtained in the Yellow Sea are put into EOF analyses. Temperature variation is predominated by the first mode which is associated with the seasonal fluctuation of temperature with annual range decreasing with the bottom depth. Since such a strong annual signal may mask the upwind or downwind flows occurring intermittently during the winter, only the data obtained during this season are put into EOF analyses. Every winter shows similar results. The first mode, explaining more than 90% of total variance, appears to be a part of the seasonal variation of temperature mentioned above. In the second mode, the time coefficient is well correlated with northerly winds to which the responses of the trough and shallow coastal areas are opposite to each other. A simple theoretical consideration suggests the following physical explanation: The northerly wind stress anomaly creates an upwind (downwind) flow over the trough (coastal) areas, which then induces a temperature increase (decrease) by advection of heat, and vice versa for the southerly wind stress anomaly. Hence, this paper provides further evidence of the intermittent upwind or downwind flows occurring in the Yellow Sea every winter.

• 한국축산시설환경학회지
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• 제17권2호
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• pp.107-114
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• 2011

본 연구는 계절과 성 분리가 생산성, 스트레스, 도체품질 및 육질에 미치는 영향을 분석하기 위하여 여름과 겨울로 계절을 나누고 각 계절별 암컷구, 거세 수컷구 및 혼합구를 설정하고 평균체중 $82.1{\pm}0.82kg$인 삼원교잡$(LY{\times}D)$ 비육돈 270두를 공시하여 비육돈 생산성과 출하 과정의 스트레스 및 도체품질과 육질을 분석하였다. 사료섭취량은 겨울에 많았고 증체 또한 겨울에 우수하였다 (p<0.05). 성 분리는 사료요구율에 영향을 미치며, 겨울과 혼합구가 양호한 것으로 나타났다 (p<0.05). 상차 후 혈중 cortisol과 CPK치가 급격하게 상승하는 것으로 나타나 상차시 극도의 스트레스가 주어지는 것으로 나타났다. 상차시 급격한 상승을 보였던 혈중 cortisol과 CPK 농도는 수송과정에 낮아지며, 3시간 계류 후에는 출하 전 수준으로 안정되었다. 상차 후 계절 관계없이 혼합구가 가장 높은 cortisol 농도를 보여 돈 방내에서의 수준을 유지하는 경향을 나타내었고 거세 수컷 혹은 암컷만으로 구성된 돈 군간에는 차이가 없었다. 도체품질은 겨울에 우수하였고 PSE 발생율 또한 겨울이 낮아지는 경향을 보였다. 여름철 거세 수컷구가 가장 높은 PSE 발생율을 나타내었으나 겨울철 혼합구는 가장 낮게 나타났다 (p<0.05). 계절과 성 분리가 돼지고기의 pH, drip loss 및 육색 등 육질에는 영향을 미치지 않았다. 이상의 결과를 요약하면 여름철에 비육돈 관리 특히 출하취급에 보다 세심한 배려가 필요하며, 성 분리는 관리자, 시설 등 농장여건에 따라 적용 여부를 결정하는 것이 타당할 것으로 판단된다.

• 한국의류산업학회지
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• 제11권1호
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• pp.174-179
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• 2009

The purpose of this study is to research the actual condition of working environment, working clothes and personal protective equipments on workers at a construction site in winter. The one to one interview by questionnaire was performed with 33 males and 2 females workers of a construction site in Daegu at $1.3^{\circ}C$ air temperature, 28% R.H., 4.7m/sec air velocity. The questionnaire consisted of 21 questions including 4 personal characteristics questions, 12 working environment/performance questions and 5 working clothes/personal protective equipments questions. It was modified from the original questionnaire developed by Finnish Institute Occupational Health. We found that physical stress of workers increased and the ability of performance decreased. The workers felt the coldest at hands/fingers, feet/toes and the face part of cheek, nose and ears. They rarely wore thermal clothing for winter and answered that the thermal gloves for winter did not provided enough warmth. Even if the workers recognized the necessity of personal protective equipments for their safety, they did not wear them for efficiency of working performance. These results will be useful elementary materials for development of working clothes and personal protective equipments from the viewpoint of improving both working performance and comfort.

• 분석과학
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• 제12권5호
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• pp.441-446
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• 1999

무우 유식물의 자엽에서 Abscisic acid(ABA). ABA plus cyeloheximide(CH)와 ABA plus polyamine(PA) 처리로 유도되는 아미노산의 변화를 분석하였다. ABA 처리로 봄무우에서는 총 아미노산의 농도가 증가되었고, 겨울무우에서는 감소되었다. 봄무우에서는 친수성 아미노산인 proiine, glycine, serine과 cysteine 등이 증가되었고(특히 proline), 반면 겨울무우에서는 cysteine, leucine와 phenylalanine이 증가되었다(특히 cysteine과 leucine). CH는 ABA가 유도시킨 탈수에 대항할 수 있는 단백질의 합성을 방해하여 아미노산의 축적을 초래하였다. PA처리는 반대로 봄무우에서는 총 아미노산의 농도가 감소되었고, 겨울무우에서는 증가되었다. 이상의 결과는 축적된 친수성 아미노산과 단백질이 스트레스에 대항하여 탈수의 적응을 유도하였고, PA은 ABA에 의한 탈수 스트레스의 적응능력을 조절한다고 본다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2015년도 학술발표회
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• pp.53-53
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• 2015

Basin-scale motions in a stratified lake rely on interactions of spatially and temporally varying wind force, bathymetry, density variation, and earth's rotation. These motions provide a major driving force for vertical and horizontal mixing of inorganic and organic materials, dissolved oxygen, storm water and floating debris in stratified lakes. In Lake Tahoe, located between California and Nevada, USA, basin-scale circulations are obviously important because they are directly associated with the fate of the suspended particulate materials that degrade the clarity of the lake. A three-dimensional hydrodynamic model, ELCOM, was applied to Lake Tahoe to investigate the underlying mechanisms that determine the characteristics of basin-scale circulations. Numerical experiments were designed to examine the relative effects of various mechanisms responsible for the horizontal circulations for two different seasons, summer and winter. The unique double gyre, a cyclonic northern gyre and an anti-cyclonic southern gyre, occurred during the winter cooling season when wind stress curl, stratification, and Coriolis effect were all incorporated. The horizontal structure of the upwelling and downwelling formed due to basin-scale internal waves found to be closely related to the rotating direction of each gyre. In the summer, the spatially varying wind field and the Coriolis effect caused a dominant anti-cyclonic gyre to develop in the center of the lake. In the winter, a significant wind event excited internal waves, and a persistent (2 week long) cyclonic gyre formed near the upwelling zone. Mechanism of the persistent cyclonic gyre is explained as a geostrophic circulation ensued by balancing of the baroclinc pressure gradient (or baroclinic instability) and Coriolis effect. Topographic effect, examined by simulating a flat bathymetry with constant depth of 300m, was found to be significant during the winter cooling season but not as significant as the wind curl and baroclinic effects.