• 농업생명과학연구
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• 제44권1호
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• pp.25-32
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• 2010

본 연구는 수분함량에 따른 보리와 호밀 silage의 발효특성을 평가하기 위하여 수행하였다. 보리와 호밀의 수분함량은 수분첨가 또는 예건을 통해 50%, 65% 및 80%로 조절하였다. 보리 silage의 pH와 Lactate:Acetate 비율은 저수분 처리구 (4.54와 6.23), 호밀 silage에서는 중수분 처리구 (4.33과 9.24)가 가장 높았다. 보리 silage의 propionate 함량은 저수분 처리구가 고수분 처리구에 비해 높았으나, 보리와 호밀 silage의 lactate, 총휘발성지방산 및 acetate 함량은 고수분 처리구가 저수분 처리구에 비해 높았다. 보리 silage의 total-N 함량은 중수분 처리구가 저수분 처리구에 비해 높게 나타났으며, 호밀 silage는 저수분 처리구가 타 처리구에 비해 높았다. 보리 silage의 $NH_3-N$ 함량과 total-N:$NH_3-N$ 비율은 고수분 처리구가 높게 나타났다. 보리와 호밀 silage의 buffering capacity는 수분함량이 높을수록 증가하였으나, pH가 높을수록 감소하였다. 보리와 호밀 silage의 수분 함량은 pH와는 부(-)의 상관관계를 나타내었으나, 휘발성지방산과 $NH_3-N$ 함량 및 total-N중 $NH_3-N$ 비율과는 정 (+)의 상관관계를 나타내었다. 이상의 결과에서 보리와 호밀 silage 제조를 위한 적정 수분함량은 70~80%로 확인되었으며, 사료작물의 수분을 60%까지로 예건하는 것은 silage 품질 저하를 초래할 수 있는 것으로 사료된다.

• 한국토양비료학회지
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• 제13권4호
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• pp.115-118
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• 1981

2년근삼(年根蔘)을 공시(供試)해서 수경조건(水耕條件)으로 Polyethylene glycol 6000을 사용(使用)하여 수분장력간(水分張力間)에 수분흡수(水分吸收) 증산(蒸散) 및 광합성량(光合成量)을 조사(調査)하여 얻은 결과(結果)를 요약(要約)하면 다음과 같다. 1. 수경액(水耕液)의 장력(張力)이 -0.5bar에서도 2시간이내(時間以內)에 20%의 광합성(光合成) 감소(減少)를 보였으며 무처리구에서도 13시간(時間)동안에 식물체의 수분(水分)은 최초수분(最初水分) 함량(含量)의 3.26% 감소(減少)되었으나 광합성량(光合成量)에는 차이(差異)가 없었다. 2. 2년근삼(年根蔘)에서 용액(溶液)의 수분장력(水分張力)(${\psi}$)과 수분(水分) 흡수량(吸收量)(W)과는 ${\psi}=\frac{-2.890}{e^{2.790W}}$의 관계를 보였으며 인삼근권토양의 영구위조점계수가 -2.890bar보마 클것으로 추정되었다. 3. 수경액(水耕液)의 수분장력(水分張力)이 감소(減少)함에 따라 증산량(蒸散量)이 현저히 감소(減少)되어 4시간후(時間後)에 -0.5bar에서도 대조구(0 bar)에 비(比)해 23.9%나 감소(減少)되었다. 4. 이상(以上)의 결과(結果) 인삼(人蔘)은 타작물(他作物)에 비(比)해 근(根)의 위조수분장력(萎凋水分張力)이 현저히 높아 수분부족(水分不足)에 대한 영향(影響)이 크며 특(特)히 한발(旱魃)이 심할때나 토양중(土壤中) 염류농도가 높을때 그 피해는 더욱 클것으로 생각된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.25-25
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• 2022

Drought becomes frequent and severe because of continuous global warming, leading to a significant loss of crop yield. In soybean (Glycine max [L.]), most of quantitative trait loci (QTLs) analyses for drought tolerance have conducted by investigating yield changes under water-restricted conditions at the reproductive stages. More recently, the necessity of QTL studies to use physiological indices responding to drought at the early growth stages besides the reproductive ones has arisen due to the unpredictable and prevalent occurrence of drought throughout the soybean growing season. In this study, we thus identified QTLs conferring wilting scores and moisture contents of soybean subjected to drought stress in the early vegetative stage using an recombinant inbred line (RIL) population derived from a cross between Taekwang (drought-sensitive) and SS2-2 (drought-tolerant). For the two traits, the same major QTL was located on chromosome 10, accounting for up to 11.5% of phenotypic variance explained with LOD score of 12.5. This QTL overlaps with a reported QTL for the limited transpiration trait in soybean and harbors an ortholog of the Arabidopsis ABA and drought-induced RING-D UF1117 gene. Meanwhile, one of important features of plant drought tolerance is their ability to limit transpiration rates under high vapor pressure deficiency in response to mitigate water loss. However, monitoring their transpiration rates is time-consuming and laborious. Therefore, only a few population-level studies regarding transpiration rates under the drought condition have been reported so far. Via employing an Arduino-based platform, for the reasons addressed, we are measuring and recording total pot weights of soybean plants every hour from the 1^st day after water restriction to the days when the half of the RILs exhibited permanent tissue damage in at least one trifoliate. Gradual decrease in moisture of soil in pots as time passes refers increase in the severity of drought stress. By tracking changes in the total pot weights of soybean plants, we will infer transpiration rates of the mapping parents and their RILs according to different levels of VPD and drought stress. The profile of transpiration rates from different levels of severity in the stresses facilitates a better understanding of relationship between transpiration-related features, such as limited maximum transpiration rates, to water saving performances, as well as those to other drought-responsive phenotypes. Our findings will provide primary insights on drought tolerance mechanisms in soybean and useful resources for improvement of soybean varieties tolerant to drought stress.

• 한국토양비료학회지
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• 제7권1호
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• pp.43-47
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• 1974

수도에 대한 비소의 피해 및 아연-비소간의 교호작용을 온실실험으로 조사하였다. 비소 피해의 증세는 수도를 시들게 하는 것이었으며 지상부 및 지하부의 생장을 감소시키었다. 비소 피해는 1 ppm의 비소 처리구에서도 관찰되었다. 아연의 시용은 수도의 비소흡수를 억제하지는 못했지만 비소 피해를 완화시키었다. 반면 처리된 비소는 아연의 흡수를 감소시키었으며, 따라서 아연시용의 효과는 비소피해에 의한 아연흡수 감소를 보상하였기 때문이다. 이 시험중 공시토양인 Crowley SiL의 비교적 높은 토양반응에 기인하여 철 결핍증이 관찰되었다.

• 생명과학회지
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• 제5권4호
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• pp.190-202
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• 1995

Two oriental melons cultivars, ‘Geumssaragi Euncheon’(GSEC) and ‘House Eunchon’(HEC), were grafted onto different rootstocks and the growth, mineral contents and fruit quality were examined. The seedlings grafted onto‘Geumtozwa’rootsticks showed about 10% defected seedling loss(wilting or dwarfing0as compared to the little or no loss in the seedlings grafted onto 'Sintozwa' or 'Chamtozwa' rootstocks. The seedings grafted onto 'Sintozwa' or 'Chamtozwa' rootstocks also exhibited better vine growth as compared to the intact seedlings or the seedlings grafted onto 'Geumtozwa' rootstocks. The fruits growth, as measured by fruits length, fruit diameter and fruit fresh weight, was accelerated by the rootstocks especially by 'Sintozwa' and 'Chamtozwa' . Intact oriental melons showed the highest soluble solids content in the fruit pericarp followed by 'Chamtozwa' rootstocks. Sucrose contents were higher in the seedling grafted onto 'Sintozwa', whereas higher glucose and fructose contents were measured in intact GSEC fruits or HEC fruits on 'Chamtozwa' rootstock. As compared to the intact plants, the seedling grafted onto 'Sintozwa' rootstock showed higher N. P and K and lower Ca and Mg contents in leaves, stems, roots and fruits. 'Geumtozwa' rootstock, developed specially for oriental melons, showed very similar pattern of mineral absorption or distribution as compared to the intact plants.

• Journal of Microbiology and Biotechnology
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• 제17권3호
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• pp.474-480
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• 2007

Fusarium wilt of cucumbers was effectively controlled by Escherichia coli expressing an endochitinase gene (chiA), and the rate was as effective (60.0%) as the wild-type strain S. proteamaculans 3095 (55.0%) where the gene was cloned. However, live cells of soil inoculated E. coli host harboring the chiA gene did not proliferate but declined 100-fold from $10^8$ CFU during the first week and showed less than 10 cells after day 14, suggesting that E. coli was able to express and produce the chitinase enzyme to the soil even as the population was gradually decreasing. Because the majority of the strains was alive for only a short period of time and the Fusarium-affected seedlings showed symptoms of wilting within 7-10 days, it seems that the pathogen control was decided early after the introduction of the biocontrol agent, eliminating the survival of the antagonist. These results indicated that soil inoculated E. coli could sufficiently express and produce the recombinant protein to control the pathogen, and root or soil colonization of the antagonist might not be a significant factor in determining the efficacy of biological control.

• 한국균학회지
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• 제45권4호
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• pp.381-385
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• 2017

Sclerotinia rot was observed on Ixeridium dentatum cultivated as a succeeding crop in a garlic field in Seosan-si, Korea during the growing season in 2016 and 2017. Symptoms progressed from the initial irregular, water-soaked spots on main stems to wilting and eventually to plant death. White, cottony mycelia and black, irregular sclerotia formed on the basal stem and on soil surfaces. The optimal temperature of hyphal growth and sclerotia germination were $20^{\circ}C$ and $25^{\circ}C$, respectively. Sequence analysis of the internal transcribed spacer (ITS) regions revealed that the three strains isolated from Ixeridium dentatum are grouped with Sclerotinia sclerotiorum. Three strains were identified as Sclerotinia sclerotiorum based on morphological features, ITS sequence, and pathogenicity test. To the best of our knowledge, this work is the first report of Sclerotinia sclerotiorum causing sclerotinia rot on Ixeridium dentatum in Korea.

• Journal of Plant Biotechnology
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• 제36권2호
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• pp.179-183
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• 2009

To understand the effect of reactive oxygen species (ROS) on floral senescence in Hibiscus syriacus L., we have investigated change in relative water potential, malondialdehyde (MDA) content, H_2O_2 content and the activity of antioxidative enzymes in the petals during flower opening and senescence. Hibiscus flowers were achieved full bloom at early morning and started to in-rolling and showed petal in-rolling over than 50% at 24 h and 36 h after full bloom, respectively. The flower was a decrease in fresh weight by 30% and showed water loss with floral senescence. MDA content and activity of antioxidative enzymes such as APX, GR and CAT were showed no significant change until 36 h after full bloom. In the flower 48 h after full bloom that showed complete petal in-rolling and wilting, however, activity of antioxidative enzymes and H_2O_2 content was greatly increased as compared with 0 h after full bloom. These results suggest that reactive oxygen species are related to accelerating the later senescence more than inducing the early senescence during Hibiscus flower senescence.

• 한국식물병리학회지
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• 제11권2호
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• pp.116-119
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• 1995

강원도 인제지역에서 집단재배되고 있는 치커리에 무름병이 관찰되었다. 병징 초기증사은 치커리 뿌리에서 무름증상을 나타내는 것을 시작으로 시간이 지남에 다라 병징부위가 점차적으로 확대되어 뿌리가 완전히 부패되었다. 뿌리의 연부증상은 치커리 지상부위를 마르게하여 결국은 고사시켰다. 병반부로부터 분리한 병원균은 생리, 화학적특성과 Biolog program의 결과에 따라 Erwinia carotovora subsp. corotovora로 동정되었다. E. carotovora subsp. carotovora에 의한 치커리의 무름병은 국내에서 보고된 바가 없기에, 본 세균에 의한 병을 "치커리 세균성무름병(bacterial soft rot of chicory)" 이라 명명할 것을 제안한다.

• 식물병연구
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• 제10권1호
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• pp.8-12
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• 2004

2003년 7월, 경상북도 봉화지역의 인삼포장에서 인삼의 줄기가 쓰러지고 뿌리썩음증상을 보이는 인삼을 관찰하였다 병든 뿌리로부터 분리된 병원세균을 접종한 결과, 인삼의 잎은 황화되거나 검은색으로 변하여 시들었고, 줄기는 속이 텅비어 있었다. 뿌리는 접종부위가 썩으면서 주름이 생기고 차츰 검은색으로 변화였다. 이 병원세균은 인삼의 모든 부위에 대해 병원성이 인정되었고 이 병원세균의 형태 및 생리적 특성을 Bergey' manual에 기록된 세균의 특성과 비교한 결과, Serratia liquefaciens로 동정되었다. 이 세균은 아직 인삼의 병원세균으로 기록되어 있지 않기에 인삼뿌리썩음병으로 이 병원세균도 추가할 것을 제안한다.