• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.272-280
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• 2016

Chrysanthemum is one of the top floriculture species with ornamental and medicinal value. Although chrysanthemum breeding program has contributed to the development of various cultivars so far, it needs to be advanced from the traditional phenotype-based selection to marker-assisted selection (molecular breeding) as shown in major cereal and vegetable crops. Molecular breeding relies on trait-linked molecular markers identified from genetic, molecular, and genomic studies. However, these studies in chrysanthemum are significantly hampered by the reproductive, genetic, and genomic properties of chrysanthemum such as self-incompatibility, inbreeding depression, allohexaploid, heterozygosity, and gigantic genome size. Nevertheless, several genetic studies have constructed genetic linkage maps and identified molecular markers linked to important traits of flower, leaf, and plant architecture. With progress in sequencing technology, chrysanthemum transcriptome has been sequenced to construct reference gene set and identify genes responsible for developments or induced by biotic or abiotic stresses. Recently, a genome sequencing project has been launched on a diploid wild Chrysanthemum species. The massive sequencing information would serve as fundamental resources for molecular breeding of chrysanthemum. In this review, we summarized the current status of molecular genetics and genomics in chrysanthemum and briefly discussed future prospects.

• Journal of Plant Biology
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• v.15 no.s
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• pp.33-49
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• 1972

Recently two thousand year old several villages ruins discovered at Amsadong, Daeshimli and Hunbaukol near the Han River side in Korea. An investigation of the present flora of these three localities was done. There are 84 families including 419 species of vascular plants. The vegetations of flora in the localities of the Han River side are very similar. the forests are poorly developed and appear as the secondary vegetations. The reforest plants can be recognized as Pinus rigida, quercus acutissima, Q. serrata, Robinia pseudoacacia and Alnus hirta. Herbaceous plants of Miscanthus sacchariflorus and Phragmitis japonicus bind up the soiles of the Han River side. Populus nigra grows abundantly in the moist places of sand bars which are located on the river side south of Daeshimli and on the northern side of the Han River across from Hunbaukol. These three area are fully cultivated but still many primitive farm techniques are employed. Farm produce crops include rice, corn, barley wheat, other vegetables, and a few ornamental plants. In this study the author found flower color variations ranging from a dark blue to a pale blue, and chromosome number differences existing between the populations of Commelina communis. The dark blue flowered taxa chromosome number is 44 and in the pale blue taxa it is 72. The wild white flowered Chrysanthemum is Chrysanthemum zawadskii Herb. ssp. acutilobum and which has finely lobed leaves, and has 54 chromosome in the root tip cells.

• Journal of the Korea Fashion and Costume Design Association
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• v.10 no.2
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• pp.1-10
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• 2008

Yellow, one of the five direction colors, is a prevailing color in artificial dyeing. The color has implied and symbolized wealth and power since ancient times. Yellow has been extracted mainly from flowers. Shapes, colors and scents of flowers not only have enriched emotional mentality, but have also been used for medicinal herbs, and dyestuff since the very old days. Coloring matter from flowers is very beautiful, and it has been used for dyeing textiles. Textile dyeing have formed a color culture and developed a dyeing culture since ancient times. Flowers include a variety of color matters, and can be easily obtained around us. Therefore, flowers have been a widespread natural dyestuff. It is well known that beautiful colors can be extracted from flowers, which are eco-friendly and non-polluting. In addition, flowers are easily provided. In this study, yellow wild flowers were selected as subjects. Seven flowers with color matters distiguishable through the naked eye were used: Korean forsythia, golden-wave, Mongolian dandelion, sunflower, conflower, chelidonium and chrysanthemum. Coloring matters were extracted from the seven flowers, which have been used to create a variety of colors through various dyestuffs; and the colors have been analyzed and presented. Dyeing and coloring matters were tested and analyzed, and several types of dyestuff had also been scientifically measured after treatment.

• Journal of Life Science
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• v.25 no.3
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• pp.299-306
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• 2015

Chrysanthemum zawadskii, a herbaceous perennial plant belonging to the Compositae, grows wild in Asian countries, including Japan, China, and Korea. The biological, antioxidative, anti-inflammatory, and antibacterial activities of C. zawadskii have been reported, its antiobesity activity has not been elucidated. In the present study, the effect of C. zawadskii methanol extract (CZME) on pancreatic lipase enzyme activity, adipocyte differentiation, and adipogenesis was investigated using an in vitro assay and a cell model system. CZME effectively suppressed lipase enzyme activity in a dose-dependent manner. CZME also inhibited insulin, dexamethasone, 3-isobutyl-1-methylxanthine (MDI)-induced adipocyte differentiation, lipid accumulation, and the level of triglyceride in 3T3-L1 preadipocytes in a dose-dependent manner, without cytotoxicity. The antiobesity effect of CZME might be modulated by gene and protein expression of cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins (C/EBP) α, C/EBPβ, and the peroxisome proliferator-activated receptor γ (PPAR γ). CZME also triggered lipolysis in a dose-dependent manner in MDI-induced 3T3-L1 preadipocytes. Taken together, these results provide important new insights into the antiobesity activities of C. zawadskii, showing that they involve pancreatic lipase inhibition, as well as antiadipogenic and lipolysis effects. CZME might be a promising source in the field of nutraceuticals. However, the active compounds that confer the antiobesity activities of CZME need to be identified.

• Food Science and Preservation
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• v.21 no.4
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• pp.529-535
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• 2014

To examine the levels of preservatives that occur naturally in food, wild plants used as commercial teas, rice cakes, or spices were studied according to the method of the Korean Food Code and analyzed with a gas chromatograph and HPLC. The levels of the natural preservatives (sodium dehydroacetate, sorbic acid, benzoic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, isopropyl p-hydroxybenzoate, propyl p-hydroxybenzoate, isobutyl p-hydroxybenzoate, butyl p-hydroxybenzoate, and propionic acid) in 21 cases were investigated against 15 kinds of wild plants. Six of 15 kinds of wild plants, such as pine needles, bamboo leaf, kudzu leaf, ramie leaf, mugwort, and nut pine leaf, were confirmed to have had sorbic acid, benzoic acid, and propionic acid. 8.201-21.839 mg/kg of benzoic acid was detected in the bamboo leaf, ramie leaf, pine needles, mugwort, kudzu leaf, and nut pine leaf. The sorbic acid contents of the bamboo leaf and the kudzu leaf were 5.630-24.995 mg/kg, respectively. The propionic acid content of the ramie leaf was 61.324-62.726 mg/kg. Nine kinds of wild plants, such as the Korean berchimia leaf, taro leaf, sasa borealis, lotus leaf, kuansh, chrysanthemum zawadskii, oak tree leaf, Chinese pepper leaf, and persimmon leaf, were not detected at the levels of the natural preservatives.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.3
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• pp.47-56
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• 2018

Research was initiated to investigate germination characteristics and germination pattern of 14 herbaceous plant entries used on the road slope during 30 days. An alternative germination condition for 14 herbaceous plant entries required by International Seed Testing Association(ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$. Significant differences were observed in the first germination rate(0.3%~40.7%)and in the final germination rate(7.7%~93.3%). Days to the first germination(2~8days), days to the 30% germination(2~6days) and days to the peak germination(6~18days) were different among 14 herbaceous plant entries in the study. From this result, we could find out higher final germination rate of 14 herbaceous plant entries in the following order; First, forage crops and cool-season turfgrasses; Second, herbeceous flowers; Third, wild plants. We could also divide germination rate among 14 herbaceous plant entries as 6 groups(I;very high, II;high, III;medium high, IV;medium low, V;low, VI;very low) based on the final germination rate and divide germination speed as 5 groups(A;very fast, B;fast, C;normal, D;slow, E;very slow)based on days to the peak germination. Considering germination characteristics and pattern of 14 herbaceous plant entries Medicago sativa, Lolium perenne, Festuca arundinacea and Cosmos sulphureus were regard as dominating species while Lespedeza cuneata, Silene armeria, Lotus corniculatus var.japonicus, Coreopsis tinctoria and Centaurea cyanus as competitive species following dominating species. However, Chrysanthemum burbankii, Pennisetum alopecuroides, Chrysanthemum boreale., Artemisia princeps var. orientalis and Arundinella hirta were not almost expected to emerge.

• Horticultural Science & Technology
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• v.30 no.2
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• pp.162-170
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• 2012

The objectives of this study were to isolate and the sequence of novel $F3'H$ gene related to an anthocyanin pathway, and to confirm the expression patterns of the gene involved in the flower color variations of chrysanthemum mutants. In this study, we isolated the full-length cDNAs and the genomic DNAs of an $F3'H$ gene from a wild type (WT) chrysanthemum (cv. Argus) and its three color mutants. The sequence analysis revealed a putative open reading frame of 1,527 bp that encodes a polypeptide of 509 amino acids. Sequence homology ranged from 97% to 99% between 'Argus' and its three color mutants. The sequence analysis from the genomic DNA revealed that the chrysanthemum $DgF3'H$ gene consisted of three exons and two introns spanning a 3,830 bp length. The sizes of the gene for three mutants ranged from a shorter size of 3,828 bp to a longer size of 3,838 bp when compared to the size of WT. The total size of the two introns was 2,157 bp for WT, but those for three color mutants ranged from 2,154 bp to 2,159 bp. A result of an RT-PCR analysis indicated that the color variations of the mutants AM1 and AM2 can be partly explained by the structural modification derived from the sequencial changes in the gene caused by gamma ray. A Southern blot analysis revealed that the $DgF3'H$ gene existing as multiple copies in the chrysanthemum genome. A systemic study will be further needed to provide a genetic mechanism responsible for the color mutation and to uncover any involvement of genetic elements for the expression of the $DgF3'H$ gene for the color variation in chrysanthemum.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.1
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• pp.64-71
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• 2006

Research was initiated to investigate vegetation coverage and growth of main herbaceous flowers under low maintenance condition like a wild state. Eleven entries were comprised of 1 annual, 2 biennials and 8 perennials from widely used herbaceous flowers in Korea. Vegetation coverage and germination rate were high on annual and biennials when compared to perennials. Callistephus chinensis showed high vegetation coverage but lasted only for a year in 2001 and disappeared in 2002 as an annual. Oenothera odorata and Oenothera lamarckiana were also high vegetation coverage species with persistent only for two years as biennials. Therefore, these species should not be used in a large amount for ecological restoration when mixed with perennials because of affecting the vegetation coverage of perennials. There were considerable variations in vegetation coverage with perennial herbaceous flowers : Lotus corniculatus and Taraxacum platycarpum-high vegetation coverage species, Platycodon grandiflorum, Aster tataricus and Aster koraiensis-medium vegetation coverage species, and Aster yomena, Patrinia scabiosaefolia and Chrysanthemum indicum-low vegetation coverage species. Growth types of herbaceous flowers tested in this research were divided into 4 types : Callistephus chinensis, Oenothera lamarckiana and Aster koraiensis-wide leaf and tall type, Aster tataricus, Patrinia scabiosaefolia, Oenothera odorata, Taraxacum platycarpum, and Aster yomena-medium leaf and medium tall type, Platycodon grandiflorum-fine leaf and dwarf type, and Lotus corniculatus-fine leaf and tall type.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.1
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• pp.38-46
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• 2002

Chrysanthemum boreale M. (hereafter, C. boreale M.), a perennial flower, has been historically used as a natural medicine in Korea. With increasing concerns for health-improving foods, the demand for C. boreale M. has become higher than ever. Howevr, the amount of wild C. boreale M. collected from mountainous areas is not enough to cover all demands. The cultivation system and fertilization strategy are required to meet increasing demand on C. boreale M. with a good quality. We investigated the effects of nitrogen application on plant growth and effective components of C. boreale M. to suggest optimum rate of nitrogen fertilization. C. boreale M. was cultivated in a pot scale (1/2000a scale), and nitrogen applied with rate of 0(N0), 50(N50), 100(N100), 150(N150), 200(N200), and $250(N250)kg\;ha^{-1}$. Phosphate and potassium were applied at the same level ($P_2O_5-K_2O=80-80kg\;ha^{-1}$) in all treatments. Maximum yield achieved in 246 and $226kg\;ha^{-1}$ N treatment on the whole plant and the flower part, a valuable part as a herbal medicine, respectively. Proline was the most abundant amino acid in the flower of C boreal M. and the contents of amino acids increased with increasing nitrogen application rate in flower. Nitrogen recovery efficiency was high more than 41% in all nitrogen treatments and increased to 61.8% in nitrogen N100 treatment. From the nitrogen content, the high nitrogen uptake, the low residue of mineral N and the reasonably good apparent fertilizer recovery, it can be inferred that C. boreale M. made efficient use of the available nitrogen. In flower, contents of Cumambrin A. which is a sesquiterpene compound and has the effect of blood-pressure reduction, decreased with increasing nitrogen application. However, the amount of Cumambrin A in flower increased as nitrogen rate increased, because of increasing flower yield. Conclusively, nitrogen fertilization could increase yields and enhance quality. The optimum nitrogen application rate might be on the range of $225{\sim}250kg\;ha^{-1}$ in a mountainous soil.

• Research in Plant Disease
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• v.17 no.3
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• pp.265-271
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• 2011

Seven phytoplasma diseases have been occurred on floricultural crops in Korea : Ph-ch1 and Ph-ch2 of chrysanthemum, Ph-lily of lily, petunia flat stem-Korean (PFS-K) of petunia, poinsettia branch inducing- Korean (PoiBI-K) of poinsettia, statis witches' broom-Korean (SWB-K) of statis and azalea witches broom (AWB). Classification of the seven phytoplasmal diseases based on 16S ribosomal RNA (rRNA) sequences showed that floricultural crop phytoplasma disease were widespread in order of aster yellow (AY), stolbur and X-disease in Korea. In phenotypic characters, the fasciation was occurred in both monocotyledon plant of lily and dicotyledon plants of petunia and poinsettia. Besides, the fascination was occurred in Ph-lily of stolbur, petunia PFS-K of AY and PoiBI-K of X-disease. This result indicated that phytoplasma classification based on 16S rRNA and symptoms are not consistently related. The comparison of 16S rRNA sequence of the seven floricultural crop phytoplasma with five tree phytoplasmal diseases of jujube witches' broom, paulownia witches' broom, wild jujube witches' broom, mulberry dwarf, golden rain phytoplasma occurred in Korea showed as high as 88.5-99.9% homology. Among them, especially mulberry dwarf showed the highest homology with the seven floricultural crop phytoplasms. Based on this result, floricultural crop phytoplasmas were assumed to be transmitted by insect vectors from tree phytoplasmas in Korea.