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Characteristics and pedigree selection of a shortened cultivation period strain in Lepista nuda (재배기간이 짧은 민자주방망이버섯 우량계통 선발 및 특성)

  • Jeon, Jong-Ock;Lee, Kwan-Woo;Lee, Kyoung-Jun;Kim, Min-Ja;Kim, In-Jae;Kim, Young-Ho
    • Journal of Mushroom
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    • v.18 no.4
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    • pp.331-338
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    • 2020
  • This study was conducted to cultivate new Lepista nuda varieties with shorter cultivation period and better fruiting body compared to that of wild strains, for mass production and commercial application. Eighteen genetic resources of L. nuda were collected and grown in boxes using rice straw-fermented growth medium. Four lines with fruiting bodies were formed and selected as cross-breeding lines. Although 657 combinations were crossed through monospore crossing, only 17 combinations were bred between the 'CBMLN-19' line and the 'CBMLN-30' line. Among them, 8 lines with fast mycelial growth and high density were selected. After inoculating the rice straw-fermented growth medium with 14 genetic resources and 8 cross-breeding lines, their incubation period was investigated. Six of the cross-breeding lines completed their incubation in 20 days, while 7 of the 14 genetic resources took more than 40 days to complete their incubation, reducing the incubation period by more than 20 days in most cross-breeding lines. After the incubations were completed, the clay loam soil was covered with for post-cultivation, and when the mycelial cultivation was complete, the formation of fruiting bodies was induced after scraping the mycelial bodies under these environmental conditions: 14℃, 95% relative humidity or higher, and 1,500 to 2,000 ppm CO2 concentration. The temperature was reduced to 6℃ at night, resulting in a low temperature shock. Thus, 4 lines of fruiting bodies occurred from two genetic resources 'CBMLN-31' and 'CBMLN-44' and two cross-bred lines 'CBMLN-96' and 'CBMLN-103'. After inoculation, the longest period for fruiting bodies to occur was 100 days for the control:, the genetic resource 'CBMLN-31', and the shortest period (45 days) was observed for the cross-breeding line 'CBMLN-103'. The result of the investigation of the fruiting body characteristics shows that the cross-bred line 'CBMLN-103' showed a small form with 1.9 g of individual weight and 123validstipes per box, which was the highest incidence among the four lines. Another cross-bred line, 'CBMLN-96', had an individual weight of 5.5 g, which is larger than that of 'CBMLN-103'; however, the number of valid stipes per box was 30 less than that of 'CBMLN-103'. Quantity analysis showed that the control, 'CBMLN-31', had the highest quantity of 783 g per box, followed by the cross-bred line, 'CBMLN-96' with 165 g per box, and then the 'CBMLN-103' with 232 g. The quantity of the two crossbred lines was lower than that of the control 'CBMLN-31'; however, the amount of fruiting bodies was higher, and the cultivation period was shortened by 32 to 33 days. Therefore, these two lines would be selected as superior lines.

Korean Ginseng in "The Veritable Records of King Sejong" (『세종실록』을 통해 본 고려인삼)

  • Joo, Seungjae
    • Journal of Ginseng Culture
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    • v.3
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    • pp.11-37
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    • 2021
  • Korean ginseng is the one of the most famous medicinal herbs globally and has long been a representative item of East Asian trade, including across China and Japan. Since Joseon (1392-1910) ginseng trade was entirely controlled by the state, The Veritable Records of the Joseon Dynasty are a valuable resource that can shed light on the history of the ginseng industry at that time. By studying the subsection "The Veritable Records of King Sejong" (世宗實錄), when ginseng was used even more widely, we assess the purpose and scale of its trade in the 15th century, identify its original listing in the geographical appendix, develop a distribution map, and explore similarities to current ginseng cultivation areas. During the reign of King Sejong (1418-1450), ginseng was sent to China as a tribute 101 times, with a combined weight of 7,060 kilograms, with less than one-third of that amount given to Japan and Okinawa. It was used to cover the travel expenses of foreign envoys and servants, but this can be seen to gradually decrease after the regnal mid-term, primarily due to a decrease in the amount of ginseng being collected. At the time, there were 113 areas of naturally growing ginseng as listed in the records' geographical appendix, including 12 recorded in the 'tributes' category: Yeongdeok-gun, Yeongju, and Cheongsong-gun in Gyeongsangbuk-do; Ulju-gun and Ulsan in Gyeongsangnam-do; Jeongeup, Wanju-gun, and Jangsu-gun in Jeollabuk-do; Hwasun-gun in Jeollanam-do; Goksan-gun and Sinpyeong-gun in Hwanghaebuk-do; Jeongju and Taecheon-gun in Pyeonganbuk-do; and Jaseong-gun and Junggang-gun in Jagang-do. A total of 101 places are recorded in the 'medicinal herbs' category, located throughout the mountains of the eight Joseon provinces, except the islands. In comparison with current ginseng cultivation sites, many of these historical areas are either consistent with or adjacent to contemporary locations. The geographical appendix to "The Veritable Records of King Sejong" was compiled in the early days of the king's reign (1432) when there was a lot of wild ginseng. The appendix is a valuable resource that indicates the possibility of growing ginseng on the Korean Peninsula in the future. The apparently natural habitats in the south, where ginseng is not currently cultivated, could be candidates for the future. Moreover, areas in the north where ginseng has not been grown, except Kaesǒng, could be a good alternative under sustainable inter-Korean exchange should cultivation sites move north due to climate warming.

A study on the impact and activation plan of unmanned aerial vehicle service (무인항공기 서비스 영향성과 활성화 방안 연구)

  • Yoo, Soonduck
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.22 no.2
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    • pp.1-7
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    • 2022
  • The purpose of this study is to discuss the impact of unmanned aerial vehicle service and how to activate it. The discussion on the impact of the introduction of the unmanned aerial vehicle service was examined in terms of economic, environmental, and social acceptance, and a plan to revitalize the industry was presented. In terms of economic impact, if transportation services are increased using unmanned aerial vehicles in the future, road-based transportation cargo may decrease and road movement speed may increase due to reduced road congestion. This can have a positive effect on the increase in the value of land or real estate assets, and it also provides an impact on smart city design. In terms of environmental impact, unmanned aerial vehicles (UAVs) generally move through electricity, so they emit less exhaust gas compared to other existing devices, such as vehicles and railroads, and thus have less environmental impact. However, noise can have a negative impact on the habitat in the presence of wild animals along their migration routes. In terms of social acceptability of unmanned aerial vehicles (UAV) technology, areas that are declining due to the emergence of new services may appear, and at the same time, organizations that create profits may appear, causing conflicts between industries. Therefore, it is essential to form a social consensus on the acceptance of emerging industries. The government should come up with various countermeasures to minimize the negative impact that reflects the characteristics of the unmanned aerial vehicle use service. Just as various systems such as road signs were introduced so that vehicles can be operated on the ground to secure air routes in the mid- to long-term for revitalization of unmanned-based industries, development and establishment of services that should be introduced and applied prior to constructing air routes I need this. In addition, the design and implementation of information collection and operation plans for unmanned air traffic management in Korea and a plan to secure a control system for each region should also be made. This study can contribute to providing ideas for mid- to long-term research on new areas with the development of the unmanned aerial vehicle industry.

African Swine Fever Outbreak in North Korea and Cooperation between South and North Korea (북한지역에서 ASF발병 현황 및 남북수의협력에 관한 연구)

  • Cho, Chung Hui
    • Journal of Appropriate Technology
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    • v.6 no.1
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    • pp.21-27
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    • 2020
  • The ASF, which originated in Africa and threatens the world, landed in Asia in 2018 in China, and became a stern threat to the security of the Korean Peninsula when North Korea officially reported the ASF to the OIE in May 2019. In 1921, Montgomery, a British veterinary pathologist, made headlines by naming the African swine fever "African Swine Fever," or ASF, a disease caused by a high fatality virus that existed in East Africa. The ASF, which was a pandemic of endemic diseases in Africa, landed in Portgal around 1957 and swept through farms in Lisbon, Portugal. The ASF continued to settle in Spain and Portugal, causing 40 years of damage until the end of the 1990s, and is now in progress after landing on the Italian island of Sardinia in 1978. The virus, which landed in Portiport of Georgia on the Black Sea coast of the Black Sea in 2007, spread to Russia and caused massive damage to China in 2018, then rapidly spread to Vietnam, Laos and Myanmar in May 2019 and spread across the country, causing massive damage to the pork industry and is now in progress. Just three months after confirming the outbreak in North Korea, the outbreak at farms in Paju and Yeoncheon was confirmed on Sept. 16, 2019, leaving South Korea with the stigma of ASF-causing countries, and although the ASF's nationwide expansion has been blocked, it is currently underway in wild boars. If the ongoing ASF in the two Koreas becomes indigenous, it would be a major disaster not only for the pork industry but also for the Korean Peninsula economy. Under the current circumstances, it is impossible to focus only on veterinary areas limited to South Korea, ruling out risk factors from the ASF outbreak. Currently, it is difficult to prevent damage to the pork industry due to the ASF outbreak due to the poor water defense reality in North Korea, and as it is adjacent to China, which has a high risk of developing various epidemic diseases, there is a need for the two Koreas to jointly conduct quarantine and quarantine on the border areas. First of all, I think rapid exchange of information and education on ASF and other diseases is necessary before establishing a joint defense system on the Korean Peninsula. It is important to conduct thorough quarantine and disinfection of ASF-generated areas in North Korea, and areas bordering China and Russia, and jointly conduct thorough quarantine and control of livestock and livestock products in circulation. Cooperation by the South and North Korean water defense industries to prevent the protracted ASF on the Korean Peninsula by all means and methods is essential.

A Study on the Distribution and Conservation Plan of Vascular Flora in Gyodong Island (교동도의 관속식물상 분포 및 보전방안 연구)

  • Yun, Ho-Geun;Kim, Sang-Jun;Lee, Jong-Won
    • Journal of Environmental Impact Assessment
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    • v.31 no.1
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    • pp.11-46
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    • 2022
  • This study was carried out to examine vascular plants and invasive alien plants in Gyodong Island, located at the northwestern Civilian Control Line (CCL) of Ganghwa-gun, Incheon, and to use them as basic data for systematic management of identified plants and establishing biodiversity conservation measures. The survey was conducted 13 times from April 2019 to August 2021. The vascularflora in Gyodong Island was identified as 109 families, 378 genera, 641 species, 15 subspecies, 49 variants, 8 forma, a total of 713 taxa. This was found to be about 15.36% of the total 4,641 taxa of vascular plants in Korea. The northern linage plants on the Korean Peninsula appearing in the Gyodong Island area were identified in 83 classification groups, including Red-based leaf edge (Carex erythrobasis H.Lev. & Vaniot). Korea endemic plants were identified as 16 taxa such as Seoul wild-ginger [Asarum heterotropoides var. seoulense (Nakai) Kitag.], and a total of 20 taxa of rare plants designated by IUCN were observed, including the endangered grade Beardless iris (Iris ruthenica Ker Gawl.). Floristic target species were classified with a total of 99 taxa. For V grade, Beardless iris 1 taxon was found. and also IV grade and III grade were identified in 8 taxa and 20 taxa respectively. The invasive alien plants identified as 75 taxa, such as Verbesina alternifolia (L.) Britton ex Kearney. The naturalization rate was 10.51%, and the urbanization index was calculated as 23.29%. Since large-scale construction has been currently underway on Hwagae Mt. in Gyodong Island as the target of survey area, the influx of invasive plants will be expected to promote. Therefore, it is urgent to establish in-situ protection and conservation measures for notable plants such as Beardless iris and Water smartweed [Persicaria amphibia (L.) S.F.Gray].

Early Growth Characteristics of Quercus rubra Associated with Soil Physicochemical Properties and Meteorological Factors in Six Regions of South Korea (토양 물리·화학적 성질 및 기상인자에 따른 국내 6개 지역의 루브라참나무 초기 생장 특성)

  • Hwang, Hwan Su;Kim, Tae Lim;Oh, Changyoung;Lim, Hyemin;Lee, Il Hwan
    • Journal of Korean Society of Forest Science
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    • v.111 no.3
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    • pp.357-364
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    • 2022
  • We investigated the early growth characteristics of Quercus rubra planted in six regions (Hwaseong, Yangpyeong, Pyeongchang, Samcheok, Chungju, and Gimje) in South Korea in relation to soil physicochemical properties and meteorological factors. Q. rubra (1-0) were planted at a density of 3,000 trees ha-1. The average height, root collar diameter (RCD), and volume of 8-year-old Q. rubra planted in 2014 were 3.52 m, 3.84 cm, and 0.0023 m3, respectively. The growth parameters of Q. rubra were the highest and lowest in Hwaseong and Pyeongchang, respectively. Correlation analysis among the soil physicochemical properties, meteorological factors, and plantation growth characteristics found that pH was the only soil factor negatively correlated with RCD, and the other soil factors were not significantly correlated with the growth characteristics. However, growth characteristics were positively correlated to average temperature from March to October and daily maximum temperature; and they were negatively correlated to altitude, topology, and the number of rainy days from March to October. In particular, the trees planted in Hwaseong area showed the best early growth characteristics because this area had the highest daily maximum temperature, the x average temperature from March to October, the low altitude, and it is located close to the foot of a mountain. In Pyeongchang, the early growth characteristics were negatively affected by winter cold damage because of the high altitude, low daily minimum temperature, and damage by wild animals. In Hwaseong, meteorological factors such as temperature and altitude were more highly correlated to growth characteristics of Q. rubra than the physicochemical soil properties. These results will provide useful information for determining suitable sites for Q. rubra plantations and for predicting early growth characteristics in response to environmental factors.

Mass Proliferation of Hibiscus hamabo Adventitious Root in an Air-lift Bioreactor, and the Antioxidant and Whitening Activity of the Extract (생물반응기를 이용한 황근 부정근의 대량증식과 추출물의 항산화 및 미백 활성 평가)

  • Lee, Jong-Du;Hyun, Ho Bong;Hyeon, Hyejin;Jang, Eunbi;Ko, Min-Hee;Yoon, Weon-Jong;Ham, Young Min;Jung, Yong-Hwan;Choi, Hwon;O, Eu Gene;Oh, Daeju
    • Korean Journal of Plant Resources
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    • v.35 no.4
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    • pp.435-444
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    • 2022
  • Hibiscus hamabo Sieb. et Zucc. (yellow hibiscus) is a deciduous semi-shrub plant and mainly growing in Jeju Island. This is known the unique wild hibiscus genus and classified as an 2nd grade of endangered plant for Korean Red List. In previous studies, properties of germination, ecological, genetical and salt resistance have been reported. In this study, we investigated mass-proliferated adventitious root using bioreactor, antioxidant and whitening effects to conduct functional ingredients. Yellow hibiscus were collected from Gujwa, Jeju by prior permission and they were introduced by explant type and various medium composition after surface sterilization. As a result, seed response rates were evaluated at range of 51.17~51.83%, in terms of comprehensive efficiency of shoot and root formation. In the case of adventitious root propagation condition was confirmed in half strength Murashige and Skoog medium salts, 30 mg/L sucrose, and 2 mg/L indole-3-butyric acid for 8 weeks in 5,000 mL bioreactor. We also compared between relationship with biomass and secondary metabolites accumulation by total phenolics content, the flavonoid content, DPPH free radical scavenging activity and melanin content. The results indicated that adventitious root mass proliferation, antioxidant and whitening effect could develop value of the high-quality cosmeceutical ingredient and further metabolite studies.

Studies on the Search for Varieties of higher Sulfur-Containing Protein with Lower Lipoxygenase Activity and their Inheritance and Selection Efficiency for the Breeding of Good Quality Soybean Cultivar 1. Search for Varieties with Higher Sulfur-Containing Amino Acids and their Inheritance and Selection Efficiency (양질콩 품종육성을 위한 고함황단백질 및 Iopoxygenase 저활성도 품종의 탐색과 그의 유전 및 선발효과 1. 고함황 아미노산 품종의 탐색과 그의 유전 및 선발효과)

  • Lee, Hong-Suk;Park, Eui-Ho;Ku, Ja-Hwan;Shim, Jae-Wook
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.6
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    • pp.499-506
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    • 1993
  • The contents of sulfur, sulfur-containing protein and amino acids of soybean seeds of 518 genotypes as well as their inheritance and selection efficiency in early breeding generation were measured to facilitate breeding for soybean with high sulfur-containing amino acids. Average seed sulfur content of 518 cultivars was 0.33%, and ranged from 0.20 to 0.45%, and that of 30 wild soybeans was 0.35%, and ranged form 0.19 to 0.62%. Correlation coefficients between seed sulfur content and sulfur-containing protein and amino acids were 0.924$^{**}$ and 0.974$^{**}$, respectively. Seed sulfur content was tended to be high in soybean genotypes with late maturity, seed coat bloom, or green cotyledon. Sulfur content had -0.312$^{**}$ correlation coeficient with sugar content and -0.384$^{**}$ with 100 seed weight. Seed sulfur content was inherited quantitatively, in which additive effect was greater than dominant one, and proportion of genes with positive effects was similar to those with negative ones. Estimated narrow- and broad-sense heritabilities were 0.75 and 0.88 for seed sulfur content, respectively. Heritability measured from selection in early breeding lines for high or low seed sulfur content was 60~62.5% or 50~62,5%, respectively. And selection for high sulfur content increased by 14.7~18.8%, whereas that for low one decreased by 8.8~15.6%, when compared to that of random population. Therefore selection in early generation seemed to be clearly effective.

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The Effects of 8-week Ketone Body Supplementation on Endurance Exercise Performance and Autophagy in the Skeletal Muscle of Mice (8주 케톤체 투여가 마우스 지구성 운동수행능력과 골격근의 자가포식에 미치는 영향)

  • Jeong-sun Ju;Min-joo Park;Dal-woo Lee;Dong-won Lee
    • Journal of Life Science
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    • v.33 no.3
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    • pp.242-251
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    • 2023
  • The purpose of this study was to investigate the effects of 8-week β-hydroxybutyrate (β-HB) administration with and without endurance exercise training on endurance exercise performance and skeletal muscle protein synthesis and degradation using a mouse model. Forty-eight male wild-type ICR mice (8 weeks old) were randomly divided into four groups: sedentary control (Sed+Con), (Sed+Con), sedentary β-HB (Sed+β-HB), exercise control (Exe+Con), and exercise β-HB (Exe+β-HB). β-HB was dissolved in PBS (150 mg/ml) and injected subcutaneously daily (250 mg/kg) for 8 weeks. Mice performed 5 days/week of a 20 min treadmill running exercise for 8 weeks. The running exercise was carried out at a speed of 10 m/min at a 10° incline for 5 min, and then the speed was increased by 1 m/min for every 1 min of the remaining 15 min. Following 8 weeks of treatments, visceral fat mass and skeletal muscle mass, blood parameters, and the markers for autophagy and protein synthesis were analyzed. The data were analyzed with one-way ANOVA (p<0.05) using the SPSS 21 program. Eight weeks of Exe+β-HB treatment significantly lowered blood lactate levels compared with the other three groups (Sed+Con, Sed+β-HB, and Exe+β-HB) Exe+β-HB) (p<0.05). Eight weeks of Exe+β-HB significantly increased maximal running time (time to exhaustion) compared with the Sed+Con and Exe+Con groups (p<0.05). Eight weeks of β-HB administration significantly decreased autophagy flux and autophagy-related proteins in the skeletal muscle of mice (p<0.05). Conversely, the combined treatment of β-HB and endurance exercise training increased protein synthesis (mTOR signaling and translation) (p<0.05). The 8-week β-HB treatment and endurance exercise training had synergistic effects on the increase in endurance performance, increase in protein synthesis, and decrease in protein degradation in the skeletal muscle of mice.

Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea (메타전사체 분석을 이용한 국내 대추나무의 바이러스 감염실태)

  • Hong-Kyu Lee;Seongju Han;Sangmin Bak;Minseok Kim;Jean Geung Min;Hak ju Kim;Dong Hyun Kang;Minhui Kim;Wonyoung Jeong;Seungbin Baek;Minjoo Yang;Taegun Lim;Chanhoon An;Tae-Dong Kim;Chung Youl Park;Jae Sun Moon;Su-Heon Lee
    • Research in Plant Disease
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    • v.29 no.3
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    • pp.276-285
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    • 2023
  • This work investigated the viral infection in jujube plants in Korea. A total of 61 samples with the symptoms of putative viral infection were collected from experimental fields and orchards. Thereafter, the samples were subjected to metatranscriptome analysis, Reverse transcription polymerase chain reaction analysis, and nucleotide sequence analysis. These analyses identified the presence of two DNA viruses, jujube-associated badnavirus (JuBV), jujube mosaic-associated virus (JuMaV), and one RNA virus, jujube yellow mottle-associated virus (JYMaV). All samples collected were confirmed to be infected by at least one of the three viruses, with most showed multiple infections. The detection rates of JuBV, JYMaV, and JuMaV were 100%, 90.2%, and 8.2%, respectively. Only three combinations of viral infections were found: 9.8% of samples showed single infection of JuBV, 82.0% showed double infection of JuBV+JYMaV, and 8.2% showed triple infection of JuBV+JYMaV+JuMaV. Sequence analysis of the three viruses showed very high homology with respective virus isolates reported in China. This study is predicted to provide fundamental data to produce virus-free jujube seedlings and represents the first report of JuBV and JuMaV infection in Korea.