• Research in Plant Disease
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• v.22 no.2
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• pp.65-71
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• 2016

Cymbidium mosaic virus (CymMV) is a major virus infecting orchid plants and causing economic loss. In this study, the incidence of viral infection in Calanthe spp. at the Korean Institute of Calanthe was investigated using reverse transcription polymerase chain reaction. The CymMV infection rate was 42%, and the two viruses Odontoglossum ringspot virus and Cucumber mosaic virus had frequencies of 8% and 2%, respectively. Additionally, we characterized an isolate of CymMV, CymMV-GW, using biological tests and examined the nucleotide sequence properties of its complete genome. CymMV-GW induced chlorotic ringspots and chlorotic spot symptoms in inoculated leaves of Chenopodium amaranticolor and Nicotiana benthamiana, respectively. In this study, we have for the first complete genome sequence of CymMV-GW in Korea. The CymMV-GW genome was 6,225 nucleotides in length, excluding the poly-(A) tail, and showed whole-genome nucleotide and amino acid sequence identities of 97.7% and 100%, respectively, with the NJ-1 isolate of CymMV. Here, we report the complete genome sequence of the CymMV-GW isolate and viral infection rates for Calanthe spp. in Korea.

• Journal of the Korean Society of Tobacco Science
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• v.9 no.2
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• pp.3-9
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• 1987

This study was been conducted to determine chemical characteristics of soils in the major districts cultivating flue-cured tobacco plant. Also native soil productivities were measured by means of bioassay planting tobacco plant without fertilizer at 87 selected lolls through pot and field experiments. Inorganic nutrient in soils affecting the dry weight of tobacco leaves cultivated in the field were investigated. The results obtained are summarized as follows; 1. Among soil chemical characteristics, pH, $NO_3$-N, $NH_4$-N , $P_2O_5$, and Mg Influenced significantly the dry weight of tobacco loaves In pot experiment, whole In the field experiments, pH, $NO_3$-N, $NO_3$-N+$NH_4$-N, and Ca had influence. 2. Correlation coefficients between soil chemical characteristics and dry weight of tobacco leaves were higher in pot experiment than field. The results revealed that soil morphological characteristics might more close influence on dry weight of tobacco leaves than chemical characteristics. 3. For prediction of dry weight (Productivity) of tobacco leaves without fertilizer multiple regression analysis were introduced using troll chemical characteristics. A combination of pH, $NO_3$-N, and Ca was very reliable for prediction of productivity as equation. y=5.02+18.07$x_1$ +2.61$x_2$ +5.36$x_3$ R=0.444** Where $x_1$ : pH, $x_2$ : $NO_3$-N, $x_3$:Ca

• Research in Plant Disease
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• v.17 no.2
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• pp.121-128
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• 2011

Bacteriolytic myxobacteria have been known to secrete various antifungal metabolites against several soilborne phytopathogens including Phytophthora. Among the three isolates of Myxococcus spp., KYC 1126 and KYC 1136 perfectly inhibited the mycelial growth of Phytophtora capsici in vitro. In order to show the biocontrol activity on Phytophthora blight of hot pepper, we tried to find the best way of application of a myxobacterial isolate. Although KYC 1126 fruiting body was easily grown on the colony of Escherichia coli as a nutrient source, it did not control the disease when it was pre-applied in soil. Before the bioassay of a liquid culture filtrate of KYC 1126 was conducted, its antifungal activity was confirmed on the seedlings applying with the mixture of the pathogen's zoospore suspension and KYC 1126 filtrate. On greenhouse experiments with five and four replications, the control value of KYC 1126 on phyllosphere and rhizosphere was 88% and 36%, respectively. Whereas, the control value of dimetnomorph+propineb on phyllosphere was 100% and that of propamorcarb on rhizosphere was 44%. There was a phytotoxicity of the myxobacterial filtrate when seedlings were washed and soaked for 24 hours. Gummy materials were covered with roots. And stem and petiole were constricted, then a whole seedling was eventually blighted.