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http://dx.doi.org/10.5423/RPD.2016.22.2.65

Survey of the Incidence of Viral Infections in Calanthe spp. and Characterization of a GW Isolate of Cymbidium mosaic virus in Korea  

Park, Chung Youl (School of Applied Biosciences, Kyungpook National University)
Baek, Da Some (School of Applied Biosciences, Kyungpook National University)
Oh, Jonghee (School of Applied Biosciences, Kyungpook National University)
Choi, Jong-Yoon (School of Applied Biosciences, Kyungpook National University)
Bae, Dae Hyeon (School of Applied Biosciences, Kyungpook National University)
Kim, Jeong-Seon (Agricultural Microbiology Division, National Academy of Agricultural Sciences, Rural Development Administration)
Jang, Gil-Hun (Korean Institute of Calanthe)
Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
Publication Information
Research in Plant Disease / v.22, no.2, 2016 , pp. 65-71 More about this Journal
Abstract
Cymbidium mosaic virus (CymMV) is a major virus infecting orchid plants and causing economic loss. In this study, the incidence of viral infection in Calanthe spp. at the Korean Institute of Calanthe was investigated using reverse transcription polymerase chain reaction. The CymMV infection rate was 42%, and the two viruses Odontoglossum ringspot virus and Cucumber mosaic virus had frequencies of 8% and 2%, respectively. Additionally, we characterized an isolate of CymMV, CymMV-GW, using biological tests and examined the nucleotide sequence properties of its complete genome. CymMV-GW induced chlorotic ringspots and chlorotic spot symptoms in inoculated leaves of Chenopodium amaranticolor and Nicotiana benthamiana, respectively. In this study, we have for the first complete genome sequence of CymMV-GW in Korea. The CymMV-GW genome was 6,225 nucleotides in length, excluding the poly-(A) tail, and showed whole-genome nucleotide and amino acid sequence identities of 97.7% and 100%, respectively, with the NJ-1 isolate of CymMV. Here, we report the complete genome sequence of the CymMV-GW isolate and viral infection rates for Calanthe spp. in Korea.
Keywords
Bioassay; Calanthe; CymMV;
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