• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.277-277
• /
• 2017

The research was carried out to investigate a total polyphenol content, antioxidant activity, amino acid and resveratrol content of peanut sprouts (cotyledon, epicotyl, leaf, hypocotyl, root), in different light (white, blue, red, F-red, UV-A, UV-B, UV-C) conditions for 24 hours. Peanut seeds were sown on a $27.5{\times}15.9{\times}13cm$ tray and grown at the $25^{\circ}C$ under the dark condition for 14 days. Total polyphenolic contents of epicotyl and leaf were about 288mg GAE/100g in blue light. The DPPH radical scavenging of cotyledon and hypocotyl were 1.3~1.5 times (63%) and 2 times (40%) compared to control (43%, 19%), respectively. As to ABTS activity, its activity was increased by all LEDs treatment, Especially, the highest ABTS activity of the hypocotyl and leaf was shown as 99.1% in blue light. The essential amino acid content of hypocotyl and leaf was increased 1.9 times in the UV-B, 1.6 times in red, and 1.5 times in F-red, respectively. The non-essential amino acid content was increased by all LEDs treatment in hypocotyl and leaf. The content of resveratrol was increased by 1.3 times in UV-B compared to that of other tissues. Assessing inclusively, this study showed that there was a significantly positive effect between increase of physiological substance activity and LED light treatment, resulting in stably producing peanut sprouts. Therefore, a material treated with LEDs is thought to be useful as a functional food resources.

• 한국버섯학회지
• /
• 제18권3호
• /
• pp.260-267
• /
• 2020

본 연구에서는 장수진흙버섯 자실체로부터 메탄올과 열수를 이용해 추출한 물질의 항당뇨, 항콜린에스테라아제, 항염증 효과를 탐색하였다. 항당뇨 실험에서 α-amylase 효소에 대한 메탄올과 열수 추출물의 저해 효과는 1.0~2.0 mg/ml의 농도에서 각각 50.67~61.33%와 52.67~65.00%를 보여 양성대조군인 acarbose의 83.67~96.33%에 비해 크게 낮았으나 α-glucosidase에 대한 메탄올과 열수 추출물의 1.0~2.0 mg/ml의 농도에서의 저해 효과는 각각 74.33~89.67%와 75.67~91.00%를 보여 53.67%와 84.67%를 보인 양성대조군 acarbose에 비해 유의하게 높아 탄수화물 분해효소인 α-glucosidase에 대한 저해 효과가 특이적으로 높았다. 메탄올과 열수 추출물의 아세틸콜린 에스테라아제에 대한 저해 효과는 1.0 mg/ml의 농도에서 각각 96.05%와 94.58%를 보여 양성대조군인 galanthamine (97.80%)과 유사하였으나 butyrylcholinesterase에 대한 저해효과는 1.0 mg/ml의 농도에서 각각 91.05%와 82.27%를 보여 galanthamine의 저해 효과 81.12%에 비해 유의하게 높았다. 염증 저해 효과 실험에서 RAW 264.7 대식세포가 배양되고 있는 배지에 메탄올과 열수 추출물을 각각 전처리 후 염증 매개 물질인 LPS를 1 ㎍/ml 처리하여 24시간 배양 후 NO 생성의 저해 효과를 조사한 결과 각각의 추출물 농도가 증가함에 따라 생성된 NO의 양도 점차 유의하게 감소하는 경향을 나타내었다. Carrageenan의 주사에 의해 흰쥐의 뒷발에 유도된 부종 저해실험에서는 투여한 메탄올 추출물의 농도가 증가함에 따라 뒷발에 유발된 부종의 용적도 농도 의존적으로 점차 감소하는 추세를 나타냈다. 따라서 장수진흙버섯의 자실체에 함유된 물질은 항당뇨, 항콜린에스테라아제 및 항염증 효과를 지니고 있어서 천연 건강식품으로의 개발 가능성이 높은 것으로 사료 된다.

• 한국수산과학회지
• /
• 제49권3호
• /
• pp.301-309
• /
• 2016

To facilitate the effective use of butter clam shell as a natural calcium resource, we determined the optimal conditions for calcium lactate (BCCL) preparation with high solubility using response surface methodology (RSM). The polynomial models developed by RSM for pH, solubility and yield were highly effective in describing the relationships between factors (P<0.05). Increased molar ratios of calcined powder (BCCP) from butter clam shell led to reduced solubility, yield, color values and overall quality. The critical values of multiple response optimization to independent variables were 1.75 M and 0.94 M for lactic acid and BCCP, respectively. The actual values (pH 7.23, 97.42% for solubility and 423.22% for yield) under optimization conditions were similar to the predicted values. White indices of BCCLs were in the range of 86.70–90.86. Therefore, organic acid treatment improved color value. The buffering capacity of BCCLs was strong, at pH 2.82 to 3.80, upon the addition of less than 2 mL of 1 N HCl. The calcium content and solubility of BCCLs were 6.2–16.7 g/100 g and 93.6-98.5%, respectively. Fourier transform analysis of infrared spectroscopy data identified BCCL as calcium lactate pentahydrate, and the analysis of microstructure by field emission scanning electron microscopy revealed an irregular form.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권4호
• /
• pp.505-513
• /
• 2018

Objective: This experiment investigated the effects of aflatoxin B1 (AFB1) alone or mixed with ochratoxin A (OTA) and/or zearalenone (ZEA) on the metabolism, immune function, and antioxidant status of dairy goats. Methods: Fifty lactating Laoshan dairy goats were randomly assigned to one of five treatment groups (n = 10) for 14 days. Goats were fed no additive (control) or administered with $50{\mu}g\;AFB1/kg$ dry matter (DM) (AFB1), $50{\mu}g\;AFB1/kg$ $DM+100{\mu}g\;OTA/kg$ DM (AFB1+OTA), $50{\mu}g\;AFB1/kg$ $DM+500{\mu}g\;ZEA/kg$ DM (AFB1+ZEA), or $50{\mu}g\;AFB1/kg$ $DM+100{\mu}g\;OTA/kg$ $DM+500{\mu}g\;ZEA/kg$ DM (AFB1+OTA+ZEA). Results: Dry matter intake and milk production were lower in goats fed AFB1+OTA+ZEA than in controls. Supplementation with AFB1, OTA, and ZEA significantly decreased red blood cell count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and mean platelet volume, and significantly increased white blood cell count, when compared with the control group. Compared with control, the combination of AFB1, OTA, and ZEA significantly increased alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities, total bilirubin (TBIL), interleukin-6, and malondialdehyde (MDA), but significantly reduced immunoglobulin A concentration, the activities of superoxide dismutase (SOD) and glutathione peroxides (GSH-Px), and total antioxidant capacity (T-AOC) in serum. Administration of AFB1 combined with OTA led to higher ALP, ALT, TBIL, and MDA, as well as lower milk production, SOD and GSH-Px activities, and T-AOC, than administration of AFB1 combined with ZEA. Conclusion: The mixture of AFB1, OTA, and ZEA exerted the greatest adverse effects on dairy goats, meanwhile the deleterious damage of the other mycotoxin combinations were in varying degrees. The findings of this study could provide guidance for the prevention and treatment of the consequences of contamination of animal feeds with combinations of mycotoxin.

• IMMUNE NETWORK
• /
• 제11권2호
• /
• pp.107-113
• /
• 2011

Background: Metabolic disorders, including type II diabetes and obesity, present major health risks in industrialized countries. AMP-activated protein kinase (AMPK) has become the focus of a great deal of attention as a novel therapeutic target for the treatment of metabolic syndromes. In this study, we evaluated whether dietary aloe could reduce obesity-induced inflammation and adipogenesis. Methods: Male C57BL/6 obese mice fed a high-fat diet for 54 days received a supplement of aloe formula (PAG, ALS, Aloe QDM, and Aloe QDM complex) or pioglitazone (PGZ) and were compared with unsupplemented controls (high-fat diet; HFD) or mice fed a regular diet (RD). RT-PCR and western blot analysis were used to quantify the expression of obesity-induced inflammation. Results: Aloe QDM complex downregulated fat size through suppressed expression of scavenger receptors on adipose tissue macrophages (ATMs) compared with HFD. Both white adipose tissue (WATs) and muscle exhibited increased AMPK activation through aloe supplementation, and in particular, the Aloe QDM complex. Obesity-induced inflammatory cytokines (IL-$1{\beta}$ and -6) and $HIF1{\alpha}$ mRNA and protein were decreased markedly, as was macrophage infiltration by the Aloe QDM complex. Further, the Aloe QDM complex decreased the translocation of NF-${\kappa}B$ p65 from the cytosol in the WAT. Conclusion: Dietary aloe formula reduced obesity-induced inflammatory responses by activation of AMPK in muscle and suppression of proinflammatory cytokines in the WAT. Additionally, the expression of scavenger receptors in the ATM and activation of AMPK in WAT led to reduction in the percent of body fat. Thus, we suggest that the effect of the Aloe QDM complex in the WAT and muscle are related to activation of AMPK and its use as a nutritional intervention against T2D and obesity-related inflammation.

• 전자공학회논문지
• /
• 제51권11호
• /
• pp.221-228
• /
• 2014

혀의 색상 및 형태는 신체의 생리적이고 임상 병리적인 상태를 반영한다. 최근에는 정량적이고 객관화된 설 진단을 위해 다양한 설 영상 측정 장치가 개발되고 있다. 설 진단의 대부분은 혀의 색상 정보를 활용하기 때문에 설 영상 획득 장치에서 조명환경의 성능은 매우 중요하다. 본 연구에서는 좁은 시스템 내부 구조에서 설 표면에 조명이 고르게 비춰질 수 있도록 간접조명을 고안하였고, 그 성능을 평가하였다. 간접 조명환경 구현을 위해 타원체 형태의 반사 구조를 시스템 내부에 위치시키고, 타원체 내부에 높은 조도의 LED 두 개를 통해 정면 카메라 아래 방향으로 빛을 조사하도록 배치시켰다. 혀 위치 영역에는 반사 구조에 의해 반사된 빛만이 조사될 수 있도록 하였다. 조명의 균질도는 5개 영역에서 밝기를 측정하여 변동계수로 평가하였고, 직접조명과 확산판조명에서 각각 0.16, 0.13으로 나타난 반면 간접조명에서는 0.01미만으로 나타났다. 혀 모형을 통해 조명에 의한 빛 반사 영역의 비율을 계산한 결과는 직접조명, 확산판조명, 간접조명에서 각각 5.76%, 4.22%, 1.79%로 나타났다. 혀 모형을 측정한 영상에서 6영역의 변동계수를 계산해 색상 균질도를 평가한 결과는 간접조명에서 0.06 미만으로 가장 우수한 것으로 나타났다. 본 논문에서 구현한 조명방식을 설진 시스템에 적용하여 진단 지표 측정의 재현성 및 반복성이 향상될 것으로 기대된다.

• Journal of Microbiology and Biotechnology
• /
• 제26권8호
• /
• pp.1383-1391
• /
• 2016

The fungal strain EML-DML3PNa1 isolated from leaf of white dogwood (Cornus alba L.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. The active fungal strain was identified as Aspergillus oryzae, which belongs to section Flavi, based on the morphological characteristics and sequence analysis of the ITS rDNA, calmodulin (CaM), and β-tubulin (BenA) genes. The strain reduced the pH value to 5.62 after 7 days of incubation. Organic acid analysis revealed the presence of citric acid (515.0 mg/kg), malic acid (506.6 mg/kg), and fumaric acid (21.7 mg/kg). The three organic acids showed moderate nematicidal activities, but the mixture of citric acid, malic acid, and fumaric acid did not exhibit the full nematicidal activity of the culture filtrate of EML- DML3PNa1. Bioassay-guided fractionation coupled with ¹H- and ¹³C-NMR and EI-MS analyses led to identification of kojic acid as the major nematicidal metabolite. Kojic acid exhibited dose-dependent mortality and inhibited the hatchability of M. incognita, showing EC₅₀ values of 195.2 μg/ml and 238.3 μg/ml, respectively, at 72 h post-exposure. These results suggest that A. oryzae EML-DML3PNa1 and kojic acid have potential as a biological control agent against M. incognita.

• 대한의용생체공학회:의공학회지
• /
• 제35권5호
• /
• pp.125-131
• /
• 2014

The purpose of this study is to verify the utility of reflected photoplethysmography sensor using two green light emitting diodes that influenced by ambient light. Recently it has been studied that green light emitting diode is suitable for light source of reflected photoplethysmography sensor at low temperature and high temperature. Another study showed that, green light is better for monitoring heart rate during motion than led light. However, it has a bad characteristic about ambient light noise. To verify the utility of reflected photoplethysmography sensor using green light emitting diode, this study measures the photoplethysmography signal that is distorted by ambient light and will propose a solution. This study has two parts of research method. One is measurement system that composed sensor and board. The sensor is made up PE-foam and Non-woven fabric for flexible sensor. The photoplethysmography signal is measured by measurement board that composed high-pass filter, low-pass filter and amplifier. Ambient light source is light bulb and white light emitting diode that has three steps brightness. Photoplethysmography signal is measured with lead II electrocardiography signal at the same time and it is measured at the finger and radial artery for 1 minute, 1000 Hz sampling rate. The lead II electrocardiography signal is a standard signal for heart rate and photoplethysmography signal that measured at the finger is a standard signal for waveform. The test is repeated 3 times using three sensor. The data is processed by MATLAB to verify the utility by comparing the correlation coefficient score and heart rate. The photoplethysmography sensor using two green light emitting diodes is shown better utility than using one green light emitting diode and red light emitting diode at the ambient light. The waveform and heart rate that measured by two green light emitting diodes are more identical than others. The amount of electricity used is less than red light emitting diode and error peak detectability factor is the lowest.

• 한국식품영양과학회지
• /
• 제44권6호
• /
• pp.888-895
• /
• 2015

패류의 주된 가공부산물인 패각은 주성분이 불용성의 탄산 칼슘으로, 이를 천연 칼슘소재로 활용하기 위해 개조개 패각의 소성분말로부터 가용성 개선 유기산(초산) 처리 칼슘 제조의 최적조건을 구명하고자 하였다. 반응표면분석법을 이용하여 제조한 11개의 시제 초산칼슘의 pH, 용해도 및 수율로부터 구명한 최적 반응조건은 초산 2.70 M 비율에 대하여 개조개 소성분말 1.05 M이었으며, 이의 최적조건을 적용한 개조개 초산칼슘의 실측 pH, 용해도 및 수율은 각각 pH 7.04, 93.0% 및 267.5%로 예측치와 유사한 결과를 얻었다. 시제 초산칼슘의 완충능은 pH 4.88~4.92 범위였으며, 칼슘 함량과 용해도는 20.7~22.8 g/100 g과 97.2~99.6%였다. FT-IR, XRD 분석 및 FESEM을 통한 미세구조는 비정형 결정으로 calcium acetate monohydrate로 확인되었다. 개조개 패각은 칼슘소재뿐만 아니라 가용성을 높인 유기산 칼슘으로 칼슘강화용 식품소재로의 이용 가능성이 높을 것으로 판단되었다.

• Ecology and Resilient Infrastructure
• /
• 제6권1호
• /
• pp.23-33
• /
• 2019

내성천은 경북 북부지역을 흐르는 모래하천으로 계절적 변동이 큰 수문학적 특성에 반응하여 발달하는 모래 하상의 역동성과 이른바 '화이트 리버'라고 하는 경관상 고유성으로 대표되는 하천이다. 하지만 2010년부터 영주댐이 건설되기 시작하고 2015년 전후로 식생이 광범위하게 활착하는 등 하천 변화가 발생하였다. 본 논문의 목적은 하천 변화의 원인이 될 수 있는 기후, 수문, 수질의 변화를 분석하고, 이에 따른 하천 변화의 가능성을 검토하기 위함이다. 분석 결과, 2015년에 1982년 다음으로 적은 강우량이 발생하였으며 이로 인해 여름철 첨두유량은 50년래 최저를 기록하였다. 내성천의 유사량 특성은 상하류가 크게 다르지 않았으나, 댐 건설과 무관하게 내성천의 연 최저수위가 지속적으로 감소하고 있었음을 확인하였다. 이는 간헐적 가뭄과 수질의 변화가 하천식생의 활착에 유리하게 작용하고, 이에 따른 물리적 변화가 하도 및 식생 활착에 영향을 준 것으로 추정된다. 그러므로 하천 변화의 원인 분석을 위해서는 하천 식생 변화와 연계한 다각적인 모니터링이 필요함을 확인하였다.