• The Plant Pathology Journal
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• v.31 no.4
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• pp.402-413
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• 2015

Yellow rust (stripe rust), caused by Puccinia striiformis f. sp. tritici, is one of the most destructive foliar diseases of wheat in Egypt and worldwide. In order to identify wheat genotypes resistant to yellow rust and develop molecular markers associated with the resistance, fifty F8 recombinant inbred lines (RILs) derived from a cross between resistant and susceptible bread wheat landraces were obtained. Artificial infection of Puccinia striiformis was performed under greenhouse conditions during two growing seasons and relative resistance index (RRI) was calculated. Two Egyptian bread wheat cultivars i.e. Giza-168 (resistant) and Sakha-69 (susceptible) were also evaluated. RRI values of two-year trial showed that 10 RILs responded with RRI value >6 <9 with an average of 7.29, which exceeded the Egyptian bread wheat cultivar Giza-168 (5.58). Thirty three RILs were included among the acceptable range having RRI value >2 <6. However, only 7 RILs showed RRI value <2. Five RILs expressed hypersensitive type of resistance (R) against the pathogen and showed the lowest Average Coefficient of Infection (ACI). Bulked segregant analysis (BSA) with eight simple sequence repeat (SSR), eight sequence-related amplified polymorphism (SRAP) and sixteen random amplified polymorphic DNA (RAPD) markers revealed that three SSR, three SRAP and six RAPD markers were found to be associated with the resistance to yellow rust. However, further molecular analyses would be performed to confirm markers associated with the resistance and suitable for marker-assisted selection. Resistant RILs identified in the study could be efficiently used to improve the resistance to yellow rust in wheat.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.205-212
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• 2009

Wheat (Triticum aestivum L.) grain texture is an important determinant of milling properties and end product use. Two linked genes, puroindoline a (PINA) and puroindoline b (PINB), control most of the genetic variation in wheat grain texture. Wheat seed proteins were examined to identify PINA and PINB gene using two pre-harvest sprouting wheat cultivars; Jinpum (resistant) and Keumgang (susceptible).Wheat seed proteins were separated by two-dimensional electrophoresis with IEF gels over pH ranges: pH 3-10. A total of 73 spots were digested with trypsin resulting peptide fragmentation were analyzed by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS). Mass spectra were automatically processed and searched through NCBInr, SWISS-PORT and MSDB database with mono isotopic masses and complete gene sequence were found by UniProt database. Puroindoline a and puroindoline b that is responsible for grain texture related with baking performance and roughness. Two spots were found Pin b (16.7 kDa) and Pin a (16.3 kDa) in Jinpum compare to seven spots were identified Pin a (16.1 kDa, 16.3 kDa) and Pin b (16.7 kDa, 9.5 kDa and 14.4 kDa) in Keumgang. Some selected spots were identified puroindoline like grain softness protein (16.9 kDa, 17 kDa and 18.1 kDa) in Keumgang. Moreover, to gain a better inferring the identification of puroindoline related proteins using proteomics, we accomplished a complete gene sequence of PINA and PINB gene in pre-harvesting sprouting wheat seeds between resistant (Jinpum) and susceptible (Keumgang).

• The Korean Journal of Food And Nutrition
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• v.29 no.5
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• pp.777-784
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• 2016

This study aimed to evaluate the quality characteristics of wheat-Makgeolli (WM), a traditional Korean cereal alcoholic drink, using three varieties of wheat, namely Jokyoung (JK), Baegjoong (BJ) and Keumkang (KK). Samples of WM brewed from 100%, 85% and 70% milling rates of the three Korean wheat cultivars were analyzed for alcohol, pH, coloring degree, total acids, soluble solid, free sugars, and organic acids. As the milling rates in wheat decreased, total sugar content in WM increased while the pH of all samples decreased. The WM exhibited 0.95~1.27% in acidity, $10.2{\sim}12.5^{\circ}Brix$ in total sugar, and 14~16% in alcohol content. The most organic acids in WM was lactic acid, ranging in all the samples from 85.3~650.3 mg%. The results showed that BJ under a 70% milling rate had the highest reducing sugar contents and 15.97% in alcohol content. The carbohydrate content increased with the milling rate of wheat. Resulting in a positive correlation between carbohydrate content of wheat and total acids, reducing sugars (p<0.001), and alcohol content (p<0.05) in WM. Total sugar content is positively correlated with alcohol and reducing sugar content (p<0.001). Considering the yield, the milling rates will be adjusted to raw material prices.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.1
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• pp.46-49
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• 1984

Ethylene evolution of leaves was measured in the samples of barley and wheat grown in the various conditions and treatments. The older and stepped leaf of barley had tended to the tendency to contain more ethylene than the younger and sound leaf. Relatively high concentration of ethylene was evolved in the samples which the detecting time was delayed and so was in the samples incubated at high temperature. The higher evolution of ethylene was determined by the barley plant grown in the field and with compost application compared to the samples grown in the pot and with composite fertilizer application, however the flooding treatment resulted in decreasing ethylene evolution comparing to untreated barley and wheat. The prostrate type and higher chrolophyll content leaves of wheat appeared to produce more ethylene than erect and lower chrolophyll leaves did.

• Journal of Applied Biological Chemistry
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• v.62 no.1
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• pp.67-71
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• 2019

All countries, including Korea, are currently experiencing the effect of rapid climate change. As a result, the cultivation area of many crops including wheat is changing, or productivity is falling sharply. If enough nitrogen is present in the soil, the increase in atmospheric carbon dioxide due to the greenhouse effect can lead to increased photosynthesis of plants, resulting in increased productivity. By contrast, a low proportion of nitrogen in soil does not increase production, often leading to the use of nitrogen fertilizers to increase crop productivity: this causes serious environmental pollution due to the leakage of nitrogen fertilizers used by crops. Increasing the understanding of the molecular level of the plant nitrogen use efficiency mechanism may contribute to increased productivity of crops and reduced of environmental pollution by nitrogen. In Korea, cultivars have developed 35 kinds of wheat, such as 'keumgang' and 'Chokyeong', which can be used for specific purposes such as baking or noodles. In this study we investigate 'keumgang' and 'Chokyeong' in order to elucidate the mechanism of nitrogen use ability of wheat and contribute to the reduction of environmental pollution by providing guidelines for the proper use of nitrogenous fertilizer.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.3
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• pp.229-238
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• 2018

The purpose of this study was to evaluate the effects of nitrogen fertilization amount and timing of application on protein content, dough properties, change in protein fraction, and bread loaf volume for Korean wheat cultivars, Baekkang, Joongmo2008, and Saekeumkang, for bread with a superior gluten composition during the grain filling stage. Protein content increased with an increase in the amount of N and timing of application. The SDS segmentation volume (SDSS) increased with an increase in N, but there was no effect of the timing of N application on SDSS. An increase in N amount and timing of application caused a difference in dough properties, such as water absorption, mixing time, and tolerance, among the cultivars. Soluble and insoluble polymeric and monomeric protein contents increased with an increase in N amount and timing of application the three Korean wheat cultivars. The effects of N amount and application timing on bread loaf volume (BLV) varied among the cultivars. The BLV of Saekeumkang increased regardless of the N amount and timing of application, but that of Baekkang and Joongmo2008 cultivars was reduced. However, there was a positive correlation between protein content with the addition of N fertilization and BLV. In addition, SDSS, mixing time, and protein fractions were positively correlated with BLV. Since the response of fertilizer conditions was different for each wheat cultivar, it is necessary to build a suitable fertilizing system for each of them. Additionally, since the environment is changing, such as abnormal climate during the maturing period, research is needed to establish appropriate fertilizer conditions for varieties of bread wheat.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.2
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• pp.152-156
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• 1984

Germination and emergence of 16 barley cultivars bred in Korea were studied under different low temperature conditions in laboratory at Suweon. This study revealed that the barley cultivars differed markedly in their germination rate index (GRI), emergence rate index (ERI), and dry matter weight. The seeds of Baekdong, Namhaebori, Gangbori and Buheung germinated better than others in $4^{\circ}C$ plot. Yeongsanbori, Kwangseong and Baekdong had very poor ERI values, but Buhobori, Namhaebori, Oweolbori, Albori and malting barley, Sacheon 6 had very good ERI values. Germination rate index were not correlated with emergence rate index under any temperature conditions. The correlation coefficient between ERI of $4^{\circ}C$ and $7^{\circ}C$ plots was very high. Those results showed that for screening the varieties or lines with good emergence capacity under low temperature conditions ERI, FS and dry matter weight would be available.

• Korean Journal of Plant Resources
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• v.14 no.3
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• pp.286-294
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• 2001

Soil-borne barley viruses were investigated in 42 regions during 1999-2003, in Korea. BaYMV, BaMMV and SbWMV symptoms were found in 37 regions. Crops resistance to mosaic viruses were investigated by growing them on 10 regions infected with soil-borne viruses. It was found that 10 unhulled barley, 6-beer bar]ey, 16 rye and 2 wheat species showed resistance to virus. Most of 15 Japanese cultivars showed infection symptoms but many of them showed relatively higher resistance in Ikasan, Youngkwang and Yesan areas region. Mixed virus infection was investigated and it was found that inter-regional species except in 10 areas, were mix infected with BaYMV and BaMMV. But in Youngkwang area all of crop species except Secheon-6 were infected only with BaYMV. Japanese cultivars were mixed infected with BaMMV and BaYMV except at Yesan that were infected only with BaYMV.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.234-234
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• 2017

Recent abnormal weather, especially continued rainfall during sowing season causes difficulty in proper sowing of wheat and delayed sowing after November 15 is concerned about freezing damage during winter, resulting in reduction of wheat yield. To correspond government policy of crop sufficiency improvement and produce and supply raw wheat and barley steadily, expansion of cultivation area is necessary and spring sowing of wheat is required. To obtain basic information on the improvement of spring sown wheat and barley production, comparison and path coefficients analysis was conducted for yield and yield related components from autumn and spring sown wheat and barley. Path analyses were known as very useful in clarifying the effects of yield components on grain yield formation, which were not accurately reflected in simple correlation anaylses. Most cultivated 5 wheat and 9 barley cultivars were sown on October and February at Cheon-ju province according to standard sowing method. For the spring sowing of wheat and barley, the varieties having vernalization degree I~III are seeded in the mid of February and seeding rate is 200~250kg/ha which is increased by 25% than autumn sowing. N-fertilizer of 95 kg/ha and the same amount of P, K dressed in autumn are applied at once as basal fertilizer. The magnitude of direct effect in each yield components on yield was in sequence as follows. In autumn wheat, grain number per $spike{\geq}$ the number of spike per $m^2$>1000-grain weight and in spring wheat, grain number per $ spike{\geq}the$ number of spike per $m^2$> 1000-grain weight. In autumn naked barley, 1000-grain weight> the number of spike per $m^2$, grain number per spike and in spring barely, the number of spike per $m^2$> grain number per spike > 1000-grain weight. In autumn covered barley, grain number per spike>the number of spike per $m^2$ and in spring coverd barley, the number of spike per $m^2$> grain number per spike, 1000-grain weight. In autumn malt barley, the number of spike per $m^2$>1000-grain weight and in spring malt barley, the direct effects of three yield components were similar. According to the path analysis of yield components for spring sown wheat and barley, it was suggested that adequate number of spike per $m^2$ was most important factor for yield increase.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.298-298
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• 2022

Milling is an important process that determines the quality of flour and is affected by milling machine type, scale, and tempering conditions. In addition, seed hardness is an important factor in determining the amount of tempering water and has been reported to affects flour yield and flour quality. There are reports that vitreousity is used as a measure to distinguish between soft and hard seeds, and the higher the vitreousity, the higher the protein contents. However, there is no established system for measuring viterousity of seeds and studies on the vitreousity and quality characteristics of flour are insufficient. Therefore, in this study, vitreousity, hardness, and milling characteristics were evaluated for 46 major domestic varieties, and their relationship was confirmed. After cutting the seeds using a seed cutter, vitreousity was measured, and seed hardness and flour particle size was measured using SKCS and PSI, respectively. As for the seed hardness index, 'Joa' was the lowest with 11.6, 'Yeonbaek' was the highest with 78.7. As for the milling yield, 'Saeol' had the lowest at 58.1%, and 'Hcjoong' had the highest at 88.6%. Seed hardness index and wheat flour production showed a high positively correlation, showing a similar to that of previous studies. Also, in flour particle size, 'Gobun' was the largest at 75.5 pm, and 'Joa' was the smallest at 43.1 um. Flour yield and flour particle size showed a high positively correlation. As a result of vitreousity, 'Hwangeumal' (55.2%), 'Saekeumkang' (59.1%), 'Baekkang' (52.3%), 'Goso' (44.6%), and 'Joa' (19.2%) were showed. Seed hardness and vitreousity showed a high positively correlation. Also as the vitreousity increased, the flour yield also showed a tendency to increase. In addition, as the seed hardness increased, particle size of the flour yield also showed a tendency to increase. It is thought that this result can be used as a measure to determine the quality of flour with vitreousity. However, further analysis of wheat varieties and methods of analyzing vitreousity are needed.