• KSBB Journal
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• v.14 no.3
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• pp.377-379
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• 1999

In order to improve the transfection efficiency of CHO/dhfr- cells using cationic lipid, optimal concentrations of the cationic lipid($LipofectAmine^{TM}$) and DNA(pEGFP-C1) need to be determined. The use of green fluorescent protein(GFP) gene as a reporter gene facilitated the quantification of transfection efficiency. The green fluorescence intensity of each cell transfected at various lipid-DNA concentrations was measured using fluorescence-activated cell sorter(FACS) analysis. A combination of $2.0{\mu}L$ cationic lipid and 0.4{$\mu}g$ DNA in a well resulted in the highest trasfection efficiency. Taken together, the method using FACS analysis of GFP is simple and fast, facilitating the optimization of transfection.

• Journal of the Korean Society for Precision Engineering
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• v.13 no.6
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• pp.52-58
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• 1996

This paper described on the effect of residual stocks in internal grinding of tungsten carbide materials in order to improve the grinding efficiency as well as grinding accuracy. Through the fundamental investigation is carried out for tungsten carbide materials using electroplated diamond wheel, the residual stock after grinding process is effective to the grinding efficiency. The obtained results are as follows: (1) Under the depth of cut(t) is constant and decreasing the workpiece velocity(Vw), the residual stock after grinding is increased, but the difference is little less than the difference by table speed. (2) Increasing the wheel velocity, the residual stock after grinding is decreased. Therefore in order to minimize the residual stock, the wheel velocity should be increased as far as possible. (3) The surface roughness and out-of roundness increased with depth of cut and table speed, and decreased with wheel velocity, but it may as well adopt as much as possible under the dimensional tolerance which is required for high efficiency grinding. (4) In order to remove residual stock, the spark-out grinding shoule be done, and it also can be improved about 20~25% throughout spark-out grinding, and the number of optimal spark-out times were within 10 times.

• Journal of Korean Society of Water and Wastewater
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• v.18 no.2
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• pp.235-241
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• 2004

The dissolved air flotation (DAF) process has been widely used for removing suspended solids with low density in water. It has been known as measuring the size of microbubbles precisely which move upward rapidly in contact zone is difficult. In this study particle counter monitoring (PCM) method is used to measure the rising microbubble after injection from a nozzle. Size and distribution curve of microbubbles are evaluated at different conditions such as pressure drop at intermediate valve, length of pipeline between saturation tank and nozzle and low pressure. And the efficiency is also checked when it collides with different size floc. The experimental results show the following fact. As the final pressure drop occurred closer to a nozzle, the bubble size became smaller. And small bubble collides with large floc as well as small one because of its physical characteristic. However large bubble collides well with large floc rather than small one since hydrodynamic flow in streamline interferes to collide between two. With performing computational process by mathematical model we have analyzed and verified the size effect between bubble and floc. Collision efficiency is the highest when P/B ratio shows in the range of 0.75 < P/B ratio ($R_{particle/Rbubble}$) < 2.0.

• Journal of Navigation and Port Research
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• v.33 no.7
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• pp.477-482
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• 2009

In this paper the order picking problem in warehouses is considered, a topic which has received considerable attention from the international academic body in recent years. The order picking problem deals with the retrieval of order items from prespecified locations in the warehouse, and its objective is usually the minimization of travel time or travel distance. Hence, a well-thought order picking policy in combination with an appropriate storage policy will enhance warehouse efficiency and reduce operational costs. This paper starts with a literature overview summarizing approaches to routing order pickers, assigning stock-keeping units to pick locations and designing warehouse layouts. Since the layout design might affect both storage and routing policies, the three factors are interdependent with respect to order picking performance. To test these interdependencies, a simulation experiment was set up, involving two types of warehouse layout, four types of storage policy, five well-known heuristics and five sizes of order picking list. Our results illustrate that from the point of view of order picking distance minimization it is recommended to equip the warehouse with a third cross aisle, although this comes at the cost of a certain space loss. Additionally, we propose a set of most appropriate matches between order picking heuristics and storage policies. Finally, we give some directions for further research and recommend an integrated approach involving all factors that affect warehouse efficiency.

• Advanced Composite Materials
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• v.17 no.1
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• pp.75-87
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• 2008

Aerospace structural applications, along with high performance marine and automotive applications, require high-strength efficiency, which can be achieved using metal matrix composites (MMCs). Rotating components, such as jet-engine blades and gas turbine parts, require materials that maximize strength efficiency and metallurgical stability at elevated temperatures. Titanium matrix composites (TMCs) are well suited in such applications, since they offer an enhanced resistance to temperature effects as well as corrosion resistance, in addition to optimum strength efficiency. The overall behavior of the composite system largly depends on the properties of the interface between fiber and matrix. Characterization of the fiber.matrix interface at operating temperatures is therefore essential for the developemt of these materials. The fiber fragmentation test shows good reproducibility of results in determining interface properties. This paper deals with the evaluation of fiber fragmentation characteristics in TMCs at elevated temperature and the results are compared with tests at ambient temperature. It was observed that tensile testing at $650^{\circ}C$ of single-fiber TMCs led to limited fiber fragmentation behavior. This indicates that the load transfer from the matrix to the fiber occurs due to interfacial friction, arising predominantly from mechanical clamping of the fiber by radial compressive residual and Poisson stresses. The present work also demonstrates that composite processing conditions can significantly affect the nature of the fiber.matrix interface and the resulting fragmentation of the fiber.

• Advances in Computational Design
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• v.1 no.3
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• pp.253-264
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• 2016

Based on the variation of strain along the cross section, any region in a structural member can be classified into two regions namely, Bernoulli's region (B-region) and Disturbed region (D-region). Since the variation of strain along the cross section for a B-region is linear, well-developed theories are available for their analysis and design. On the other hand, the design of D-region is carried out based on thumb rules and past experience due to the presence of nonlinear strain distribution. Strut-and-Tie method is a novel approach that can be used for the analysis and design of both B-region as well as D-region with equal importance. The strut efficiency factor (${\beta}_s$) is needed for the design and analysis of concrete members using Strut and Tie method. In this paper, equations for finding ${\beta}_s$ for bottle shaped struts in concrete deep beams (a D-region) with and without steel fibres are developed. The effects of transverse reinforcement on ${\beta}_s$ are also considered. Numerical studies using commercially available finite element software along with limited amount of experimental studies were used to find ${\beta}_s$.

• Journal of Korea Water Resources Association
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• v.32 no.2
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• pp.121-129
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• 1999

When the soil vapor extraction as a remediation method of contaminated soil and groundwater has been used, the effects of curtain wall, mode of pump operation and magnitude of extraction flowrate were examined by numerical simulation. Consequently, it was found that the removal rate was enhanced in case that the curtain wall was established around the extraction well with the extraction pumps operated alternatively. It was because that the removal of high density gas around the extraction well was possible. It was found that the removal efficiency of TCE gas did not depend on the extraction flowrate. However, the removal rate of TCE gas at varying extraction flowrate was not enhanced flowrate increase.

• International Journal of Computer Science & Network Security
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• v.22 no.9
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• pp.414-426
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• 2022

Elliptic Curve Cryptography (ECC) is one of the finest cryptographic technique of recent time due to its lower key length and satisfactory performance with different hardware structures. In this paper, a High Throughput Multiplier architecture is introduced for Elliptic Cryptographic applications based on concurrent computations. With the aid of the concurrent computing approach, the High Throughput Concurrent Computation (HTCC) technology that was just presented improves the processing speed as well as the overall efficiency of the point-multiplier architecture. Here, first and second distinct group operation of point multiplier are combined together and synthesised concurrently. The synthesis of proposed HTCC technique is performed in Xilinx Virtex - 5 and Xilinx Virtex - 7 of Field-programmable gate array (FPGA) family. In terms of slices, flip flops, time delay, maximum frequency, and efficiency, the advantages of the proposed HTCC point multiplier architecture are outlined, and a comparison of these advantages with those of existing state-of-the-art point multiplier approaches is provided over GF(2¹⁶³), GF(2²³³) and GF(2²⁸³). The efficiency using proposed HTCC technique is enhanced by 30.22% and 75.31% for Xilinx Virtex-5 and by 25.13% and 47.75% for Xilinx Virtex-7 in comparison according to the LC design as well as the LL design, in their respective fashions. The experimental results for Virtex - 5 and Virtex - 7 over GF(2²³³) and GF(2²⁸³)are also very satisfactory.

• Korea Journal of Hospital Management
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• v.7 no.2
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• pp.52-79
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• 2002

The hospital is characterized by it's remarkable labor industry and human resources input by unit. Recently, the administrative personnel are recognized as an important staff to provide a hospital guidance to consumer and also easiness for consumer's visit to hospital. The purpose of this study is to find the organizational effectiveness of the administrative personnel in hospitals. The survey data involved in the study was derived from 229 personnels working in 3 medium and small sized hospitals and 1 university hospital in Inchon area. The major finding are as follows; 1. The organizational efficiency in accordance with the general characteristics of subjects in order of over 31 years of age, university graduates, long-term tenure and high position is higher, whereas, in as much as wage and well trained personnels in are higher, general hospital's organizational efficiency is higher in comparison with those of university hospital. 2. The organizational efficiency in accordance with satisfaction and the psychological motive contributional factors is higher as much as the high satisfactory level in every hospitals in general. 3. The organizational efficiency in accordance with the factors of job characteristics is higher in as much as difficulty of the jobs is lesser, however there was not statically significance. In as much as job standard level is higher and the more job responsibility the higher organizational efficiency. It was obvious that the higher professional expertise as well as the training and application level are improving the organizational efficiency. 4. The organizational efficiency in accordance with the factors of structural characteristics was higher in as much as the intercommunication was smooth and the structural formalization level are higher, however there was not statically significance between the participation level of decision making and the organizational efficiency. 5. In as much as older age, management of organization and the job level are satisfied, the higher structural formalization level, the smooth intercommunication have affected as major influence factors of organizational efficiency. 6. In the university hospitals is satisfied in the management and job level of hospital organization as there are no difficult jobs while the level of hospital's organizational formality is high and the intercommunication is smooth, which are improving the organizational efficiency. In the general hospitals is also satisfied the management and job level of hospital organization and psychological motive contributional factors is higher, it was apparent that the organizational efficiency is higher in as much as the level of job standardization is high and the intercommunication is smooth. As a result of this study, in order for improving the organizational efficiency of administrative personnel in hospitals, the management and job level as well as personal relation are preferably satisfied, whereas formalization of organization, intercommunication and etc. should be satisfied, and, therefore, it is advisable to buildup discriminated organizational management and environment for different division on the basis above factors. Since this study is carried on four hospitals in Inchon area, there is a certain limit to generalize its result to all domestic hospitals, nevertheless the gallop poll was made by developing the questionnaires with reasonability and reliability. Especially. as the study was carried by analyzing the comparison of influence factors' difference of organizational efficiency in accordance with the divisional characteristics of the university and general hospitals.

• The Journal of Engineering Geology
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• v.27 no.4
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• pp.405-416
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• 2017

This study evaluated a model unconfined aquifer comprising a sand or gravel layer, a filter layer, a pumping well, and an observation well. The model was employed in step drawdown tests and then used to assess the permeability of each test tank. The optimal yield and well efficiency were then calculated. Evaluation of yield by step in sand layer filters of equal thickness gave optimized watering rates of 22.03 L/min in the double filter and 19.71 L/min in the single filter. The double filter's yield was 115.0% that of the single filter. A comparison of double and single filters, each 10 cm thick, showed the double filter to have a maximum yield of 182.7%. Yields for the gravel layer were 73.56 L/min for a double filter and 65.47 L/min for a single filter of the same thickness; the former value is 112.3% of that of the latter. Comparison of double and single filters with 10-cm-thick gravel layers revealed that the double filter had a maximum yield of 160.9%. Results for sand wells showed the double filter to have a maximum efficiency of 70.4% and the single filter to have a minimum efficiency of 37.1%. Gravel-layer well efficiencies were >66.5% for both double and single filters (each 30 cm thick), but only 22.5% for a 10-cm-thick single filter. This study confirms that permeability improved as the filter material became thicker; it also shows that a double filter has a higher yield and well efficiency than a single filter. These results can be applied to the practical design of wells.