• Journal of Microbiology and Biotechnology
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• 제6권6호
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• pp.375-380
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• 1996

The gene coding for glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase was cloned from Pseudomonas sp. GK16 and some of its characteristics were analyzed. The complete nucleotide sequence revealed that the putative open reading frame is 2160 bases long and encodes 720 amino acids. By SDS-PAGE three proteins, approximately corresponding to 70, 54 and 16 kDa of molecular weight, were detected in E. coli cells carrying pGAP18. The largest protein should be a precursor which is not processed yet, while the other two proteins must be derived from the precursor by the proteolytic processing.

• 한국미생물·생명공학회지
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• 제24권4호
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• pp.415-422
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• 1996

A gene coding for a pyruvyl transferase enzyme involved in exopolysaccharide biosynthesis of Zoogloea ramigera 115SLR was isolated and sequenced. A 4.5 kb of BamHI DNA fragment was isolated from chromosomal DNA using a probe derived from ketal pyruvyl transferase gene of Xanthomonas campestris. The nucleotide sequence of 2.66 kb Pst1/HindIII DNA fragment which was homology with a probe revealed the existence of two complete open reading frames (ORF2 and ORF3) and two partial open reading frames (ORFI and ORF4). The deduced amino acid sequence of ORF3 was homologous to the ketalase (GumL product) of X campestris with 49.5% of similarity and 21.6% of identity. ORF2 on the other hand showed the higher identity with the ketalase (ExoV product) of Rhizobium meliloti (36%) as well as the ketalase of X campestris (23%) than that of ORF3. A gene product of ORF2 was determined with a bacteriophage T7 RNA polymerase/promoter system in E. coli. The molecular weight of protein was 33,500 dalton.

• Journal of Korean Neurosurgical Society
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• 제58권5호
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• pp.476-478
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• 2015

Isolated cortical vein thrombosis (ICVT) is a rare disease, accounting for less than 1% of strokes. A 46-year-old woman presented with progressive left side weakness. Magnetic resonance (MR) imaging with $T2^*$-gradient echo ($T2^*$-GE) sequence showed long cord sign at the right frontal cortex. The patient was treated with low molecular weight heparin, followed by oral warfarin for 6 months. The 3-month follow-up MR imaging showed recanalization of the previously thrombosed cortical vein. She was completely recovered without neurological deficits after 6 months. This provides that MR imaging with $T2^*$-GE sequence can help to diagnosis the ICVT and outcomes of the ICVT are generally favorable.

• 한국통신학회논문지
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• 제18권8호
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• pp.1128-1135
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• 1993

본논문에서는 GMW시퀸스 g(t) = tr((trk(a))r)와 그의 순회 천이 시\ulcorner스에의해서 발생되는 GMW부호라는 새로운 이진순회부호가 소개되었다. GMW부호의 부호깊이는 2k-1인데 여기서 k는 복합정수 e.j이다. GMW부호의 차원은 k.(k/J)w-1인데, 여기서 w는 r의 해밍무게이다. 디자인 거리, 최소거리, 그리고 부호의 무게가 GMW시퀸스의 변수에의해 유도되었다. 그리고 GMW시퀸스의 확장이 m시퀸스와 그의 순회 천이 시퀸스들의 견지에서 유도되었고. GMW시퀸스의 특성 다항식이 유도되었다.

• 산업경영시스템학회지
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• 제31권3호
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• pp.17-23
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• 2008

In this research article, scheduling a casting sequence in a job-shop type foundry involving a variety of casts made of an identical alloy but with different shapes and II weights, has been investigated. The objective is to produce the assigned mixed orders satisfying due dates and obtaining the highest ingot efficiency simultaneously. Implementing simple integer programming instead of complicated genetic algorithms accompanying rigorous calculations proves that it can provide a feasible solution with a high accuracy for a complex, multi-variable and multi-constraint optimization problem. Enhancing the ingot efficiency under the constraint of discrete ingot sizes is accomplished by using a simple and intelligible algorithm in a standard integer programming. Employing this simple methodology, a job-shop type foundry is able to maximize the furnace utilization and minimize ingot waste.

• Journal of Microbiology and Biotechnology
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• 제5권5호
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• pp.269-273
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• 1995

A thermotolerable restriction endonuclease. Svil, found in Streptomyces violochromogenes D2-5 was purified. For the purification, streptomycin sulfate and ammonium sulfate precipitation was used. Ph osphocellulose P-ll, DEAE-Cellulose and Sephacryl-S200 HR colum chromatography were also performed. The purified enzyme was found to be homogeneous and the molecular weight of the enzyme estimated by polyacrylamide gel electrophoresis containing 0.1$%$ SDS was about 32, 000 daltons. The recognition sequence and cleavage site of the enzyme were determined to be $5^1$-$TT\downarrow CGAA$-$3^1$ which is the same sequence as that of Asull. Unlike Asull, however, the Svil shows high thermal stability.

• 한국양식학회지
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• 제16권3호
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• pp.165-170
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• 2003

Isolation, cloning and sequencing of red seabream (Pagrus major) somatolactin (rsbSL) cDNA from pituitary gland revealed an open reading frame of 693 bp coding for a pre-growth hormone of 231 amino acids with a 22 amino acid putative signal peptide. Deduced amino acid sequence showed that there was one possible N-glycosylation site at Asn$^{145}$ and seven Cys residues (Cys$_{29}$ , Cys$^{39}$ , Cys$^{66}$ , Cys$^{89}$ , Cys$^{205}$ , Cys$^{222}$ , Cys$^{230}$ ). Except Cys$^{66}$ , others may be involved in disulfide bond formation. The rsbSL presented a 93% amino acid sequence identity with the SL of gilthead seabream (Sparus aurata) and contained the conserved hormone domain region. Expression of rsbSL in E. coli (BL2l) cells and gel analysis revealed a higher molecular weight for rsbSL than expected theoretically, implying posttranslational modifications.

• 방사선산업학회지
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• 제8권2호
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• pp.65-69
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• 2014

The cellobiohydrolase gene (cbhI), a key component of a cellulolytic system, of a mutant PfCM4 (Pleurotus florida), developed through gamma ray radiation mutagenesis, was isolated and cloned. The deduced amino acid sequence was closely related to the glycoside hydrolase family 7 (GH7). The molecular weight of the deduced amino acid sequence of cbhI gene was found to be 22.4 kDa. Though the percent identity was found to be much less (35.61%) between the wild type and mutant, the cellulolytic activity of PfCM4 was 17.24% higher than that of the wild type. This shows that the catalytic domain of the cbhI gene was conserved in the mutant PfCM4.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제3권6호
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• pp.612-627
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• 2009

The high-level contribution of this paper is to illustrate the effectiveness of using graph theory tree traversal algorithms (pre-order, in-order and post-order traversals) to generate the chain of sensor nodes in the classical Power Efficient-Gathering in Sensor Information Systems (PEGASIS) data aggregation protocol for wireless sensor networks. We first construct an undirected minimum-weight spanning tree (ud-MST) on a complete sensor network graph, wherein the weight of each edge is the Euclidean distance between the constituent nodes of the edge. A Breadth-First-Search of the ud-MST, starting with the node located closest to the center of the network, is now conducted to iteratively construct a rooted directed minimum-weight spanning tree (rd-MST). The three tree traversal algorithms are then executed on the rd-MST and the node sequence resulting from each of the traversals is used as the chain of nodes for the PEGASIS protocol. Simulation studies on PEGASIS conducted for both TDMA and CDMA systems illustrate that using the chain of nodes generated from the tree traversal algorithms, the node lifetime can improve as large as by 19%-30% and at the same time, the energy loss per node can be 19%-35% lower than that obtained with the currently used distance-based greedy heuristic.

• 정보과학회 논문지
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• 제44권8호
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• pp.842-853
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• 2017

스트리밍 데이터는 시간에 따라 지속적으로 생성되는 데이터 시퀀스이다. 시간이 지남에 따라 데이터의 분포 또는 컨셉이 변화할 수 있으며, 이러한 변화는 분류 모델의 성능을 저하시키는 요인이 된다. 점층적 적응적 학습 방법은 컨셉 변화의 정도에 따라 현재 분류 모델의 가중치를 조절하여 업데이트를 수행함으로써 컨셉 변화에 대한 분류 모델의 성능을 유지할 수 있게 한다. 그러나, 컨셉 변화의 정도에 맞는 적절한 가중치를 결정하기가 어렵다는 문제점이 있다. 본 논문에서는 컨셉 변화에 따른 적응적 가중치 조정에 기반한 동적 앙상블 방법을 제안한다. 실험 결과는 제안한 방법이 다른 비교 방법들에 비해 높은 성능을 보여줌을 입증한다.