Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
권호 표지

Cloning of Cellobiohydrolase Gene (cbhI) in Radiation Induced Mutant of Pleurotus florida

  • Lee, Young-Keun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Sathesh-Prabu, Chandran (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Min Kyung (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • Received : 2014.06.16
  • Accepted : 2014.08.24
  • Published : 2014.09.30

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Abstract

The cellobiohydrolase gene (cbhI), a key component of a cellulolytic system, of a mutant PfCM4 (Pleurotus florida), developed through gamma ray radiation mutagenesis, was isolated and cloned. The deduced amino acid sequence was closely related to the glycoside hydrolase family 7 (GH7). The molecular weight of the deduced amino acid sequence of cbhI gene was found to be 22.4 kDa. Though the percent identity was found to be much less (35.61%) between the wild type and mutant, the cellulolytic activity of PfCM4 was 17.24% higher than that of the wild type. This shows that the catalytic domain of the cbhI gene was conserved in the mutant PfCM4.

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Acknowledgement

Supported by : Ministry of Science, ICT and Future Planning (MSIP)