Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Molecular Cloning of Red Seabream, Pagrus major Somatolactin cDNA and Its Expression in Escherichia coli

  • Munasinghe, Helani (Department of Marine Biotechnology, Cheju National University) ;
  • Koh, Soon-Mi (Department of Marine Biotechnology, Cheju National University) ;
  • Lee, Jehee (Department of Marine Biotechnology, Cheju National University)
  • Published : 2003.08.01
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Abstract

Isolation, cloning and sequencing of red seabream (Pagrus major) somatolactin (rsbSL) cDNA from pituitary gland revealed an open reading frame of 693 bp coding for a pre-growth hormone of 231 amino acids with a 22 amino acid putative signal peptide. Deduced amino acid sequence showed that there was one possible N-glycosylation site at Asn$^{145}$ and seven Cys residues (Cys$_{29}$ , Cys$^{39}$ , Cys$^{66}$ , Cys$^{89}$ , Cys$^{205}$ , Cys$^{222}$ , Cys$^{230}$ ). Except Cys$^{66}$ , others may be involved in disulfide bond formation. The rsbSL presented a 93% amino acid sequence identity with the SL of gilthead seabream (Sparus aurata) and contained the conserved hormone domain region. Expression of rsbSL in E. coli (BL2l) cells and gel analysis revealed a higher molecular weight for rsbSL than expected theoretically, implying posttranslational modifications.

Keywords

References

  1. J. Endocrinol. v.156 Recombinant somatolactin as a stable and bioactive protein in a cell culture bioassay: development and validation of a sensitive and reproducible radioimmunoassay Calduch-Giner, J. A.;C.Pendon;M.M.Valdivia;J. Perez-Sanchez https://doi.org/10.1677/joe.0.1560441
  2. Comp. Biochem. Physiol. C v.130 Somatotropic regulation of fish growth and adiposity: growth hormone (GH) and somatolactin (SL) relationship Company,R.;A.Astola;C.Pendon;M.M.Valdivia;J. Perex-Sanchez https://doi.org/10.1016/S1096-4959(01)00408-0
  3. Gen. Comp. Endocrinol. v.91 Activation of somatolactin cells in the pituitary of the rainbow trout Oncorhynchus mykiss by low environmental calcium Kakizawa,S.;T.Kaneko;S.Hasegawa;T.Hirano https://doi.org/10.1006/gcen.1993.1130
  4. Gen. Comp.Endocrinol v.98 Effects of feeding, fasting, background adaptation, acute stress and exhaustive exercise on the plasma somatolactin concentrations in rainbow trout Kakizawa,S.;T.Kaneko;S.Hasegawa;T.Hirano https://doi.org/10.1006/gcen.1995.1054
  5. J. Exp. Biol. v.199 Elevation of plasma somatolactin concentrations during acidosis in rainbow trout Kakizawa,S.;T.Kaneko;T.Hirano
  6. Gen. Comp. Endocrinoll v.92 Pituitary of cobalt variant of the rainbow trout separated from the hypothalamus lacks most pars intermedial and neurohypophysial tissue Kaneko,T.;S.Kakizawa;T.Yada https://doi.org/10.1006/gcen.1993.1140
  7. Protein Engineering v.10 Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites Nielsen,H.;J.Engelbrecht;S.Brunak;G. von Heijne https://doi.org/10.1093/protein/10.1.1
  8. Int. J. Neural Sys. v.8 A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites Nielsen,H.;J.Engelbrecht;S.Brunak;G. von Heijne https://doi.org/10.1142/S0129065797000537
  9. Gen. Comp. Endocrinol. v.93 Immunocytochemical study of the somatolactin cells in the pituitary of Pacific salmon, Oncorhynchus nerka and O. keta at some stages of the reproductive cycle Olivereau,M.;M. Rand-Weaver https://doi.org/10.1006/gcen.1994.1004
  10. Oncorhynchus kisutch.Gen.Comp.Endocrinol. v.87 Somatolactin stimulates in vitro gonadal steroidogenesis in coho salmon Planas,J.V.;P.Swanson;M.Rand-Weaver;W.W.Dickhoff https://doi.org/10.1016/0016-6480(92)90142-7
  11. Biochemistry v.30 Isolation and characterization of somatolactin, a new protein related to growth hormone and prolactin from Atlantic cod (Gadus morhua) pituitary glands Rand-Weaver, M.;T.Noso;K.Muramoto;H.Kawauchi https://doi.org/10.1021/bi00220a010
  12. Fish. Physiol. Biochem. v.11 Plasma somatolactin levels in coho salmon (Oncorhynchus kisutch) during smoltification and sexual maturation Rand-Weaver, M.;T.G.Pottinger;J.P.Sumpter https://doi.org/10.1007/BF00004564
  13. J. Endocrinol. v.138 Plasma somatolactin concentrations in salmonid fish are elevated by stress Rand-Weaver, M.;T.G.Pottinger;J.P.Sumpter https://doi.org/10.1677/joe.0.1380509
  14. Gen. Comp. Endocrinol v.99 Red drum somatolactin: Development of a homologous radioimmunoassay and plasma levels after exposure to stressors or various backgrounds Zhu,Y.;P.Thomas https://doi.org/10.1006/gcen.1995.1111