• Journal of Microbiology and Biotechnology
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• v.6 no.6
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• pp.375-380
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• 1996

The gene coding for glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase was cloned from Pseudomonas sp. GK16 and some of its characteristics were analyzed. The complete nucleotide sequence revealed that the putative open reading frame is 2160 bases long and encodes 720 amino acids. By SDS-PAGE three proteins, approximately corresponding to 70, 54 and 16 kDa of molecular weight, were detected in E. coli cells carrying pGAP18. The largest protein should be a precursor which is not processed yet, while the other two proteins must be derived from the precursor by the proteolytic processing.

• Microbiology and Biotechnology Letters
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• v.24 no.4
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• pp.415-422
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• 1996

A gene coding for a pyruvyl transferase enzyme involved in exopolysaccharide biosynthesis of Zoogloea ramigera 115SLR was isolated and sequenced. A 4.5 kb of BamHI DNA fragment was isolated from chromosomal DNA using a probe derived from ketal pyruvyl transferase gene of Xanthomonas campestris. The nucleotide sequence of 2.66 kb Pst1/HindIII DNA fragment which was homology with a probe revealed the existence of two complete open reading frames (ORF2 and ORF3) and two partial open reading frames (ORFI and ORF4). The deduced amino acid sequence of ORF3 was homologous to the ketalase (GumL product) of X campestris with 49.5% of similarity and 21.6% of identity. ORF2 on the other hand showed the higher identity with the ketalase (ExoV product) of Rhizobium meliloti (36%) as well as the ketalase of X campestris (23%) than that of ORF3. A gene product of ORF2 was determined with a bacteriophage T7 RNA polymerase/promoter system in E. coli. The molecular weight of protein was 33,500 dalton.

• Journal of Korean Neurosurgical Society
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• v.58 no.5
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• pp.476-478
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• 2015

Isolated cortical vein thrombosis (ICVT) is a rare disease, accounting for less than 1% of strokes. A 46-year-old woman presented with progressive left side weakness. Magnetic resonance (MR) imaging with $T2^*$-gradient echo ($T2^*$-GE) sequence showed long cord sign at the right frontal cortex. The patient was treated with low molecular weight heparin, followed by oral warfarin for 6 months. The 3-month follow-up MR imaging showed recanalization of the previously thrombosed cortical vein. She was completely recovered without neurological deficits after 6 months. This provides that MR imaging with $T2^*$-GE sequence can help to diagnosis the ICVT and outcomes of the ICVT are generally favorable.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.18 no.8
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• pp.1128-1135
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• 1993

In this paper, new binary cyclic codes (hereafter, refered to as GMW code) which are generated by using GMW sequence, g(t) = tr((trk(a))r), and its cyclic shifts are introduced. Code length of GMW codes is 2a-1, where k is composite integer, e·J. Dimension of the GMW codes is k(k/j)w-1, where w is a Hamming weight of r. Several properties of GMW codes such as designed distance, minimum distance, and weights of code words are obtained in terms of parameters of GMW sequences. And expansion of GMW sequences in terms of m-sequence and its decimation sequences are introduced and characteristic polynomials of GMW sequences are also derived.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.31 no.3
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• pp.17-23
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• 2008

In this research article, scheduling a casting sequence in a job-shop type foundry involving a variety of casts made of an identical alloy but with different shapes and II weights, has been investigated. The objective is to produce the assigned mixed orders satisfying due dates and obtaining the highest ingot efficiency simultaneously. Implementing simple integer programming instead of complicated genetic algorithms accompanying rigorous calculations proves that it can provide a feasible solution with a high accuracy for a complex, multi-variable and multi-constraint optimization problem. Enhancing the ingot efficiency under the constraint of discrete ingot sizes is accomplished by using a simple and intelligible algorithm in a standard integer programming. Employing this simple methodology, a job-shop type foundry is able to maximize the furnace utilization and minimize ingot waste.

• Journal of Microbiology and Biotechnology
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• v.5 no.5
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• pp.269-273
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• 1995

A thermotolerable restriction endonuclease. Svil, found in Streptomyces violochromogenes D2-5 was purified. For the purification, streptomycin sulfate and ammonium sulfate precipitation was used. Ph osphocellulose P-ll, DEAE-Cellulose and Sephacryl-S200 HR colum chromatography were also performed. The purified enzyme was found to be homogeneous and the molecular weight of the enzyme estimated by polyacrylamide gel electrophoresis containing 0.1$%$ SDS was about 32, 000 daltons. The recognition sequence and cleavage site of the enzyme were determined to be $5^1$-$TT\downarrow CGAA$-$3^1$ which is the same sequence as that of Asull. Unlike Asull, however, the Svil shows high thermal stability.

• Journal of Aquaculture
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• v.16 no.3
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• pp.165-170
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• 2003

Isolation, cloning and sequencing of red seabream (Pagrus major) somatolactin (rsbSL) cDNA from pituitary gland revealed an open reading frame of 693 bp coding for a pre-growth hormone of 231 amino acids with a 22 amino acid putative signal peptide. Deduced amino acid sequence showed that there was one possible N-glycosylation site at Asn$^{145}$ and seven Cys residues (Cys$_{29}$ , Cys$^{39}$ , Cys$^{66}$ , Cys$^{89}$ , Cys$^{205}$ , Cys$^{222}$ , Cys$^{230}$ ). Except Cys$^{66}$ , others may be involved in disulfide bond formation. The rsbSL presented a 93% amino acid sequence identity with the SL of gilthead seabream (Sparus aurata) and contained the conserved hormone domain region. Expression of rsbSL in E. coli (BL2l) cells and gel analysis revealed a higher molecular weight for rsbSL than expected theoretically, implying posttranslational modifications.

• Journal of Radiation Industry
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• v.8 no.2
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• pp.65-69
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• 2014

The cellobiohydrolase gene (cbhI), a key component of a cellulolytic system, of a mutant PfCM4 (Pleurotus florida), developed through gamma ray radiation mutagenesis, was isolated and cloned. The deduced amino acid sequence was closely related to the glycoside hydrolase family 7 (GH7). The molecular weight of the deduced amino acid sequence of cbhI gene was found to be 22.4 kDa. Though the percent identity was found to be much less (35.61%) between the wild type and mutant, the cellulolytic activity of PfCM4 was 17.24% higher than that of the wild type. This shows that the catalytic domain of the cbhI gene was conserved in the mutant PfCM4.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.3 no.6
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• pp.612-627
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• 2009

The high-level contribution of this paper is to illustrate the effectiveness of using graph theory tree traversal algorithms (pre-order, in-order and post-order traversals) to generate the chain of sensor nodes in the classical Power Efficient-Gathering in Sensor Information Systems (PEGASIS) data aggregation protocol for wireless sensor networks. We first construct an undirected minimum-weight spanning tree (ud-MST) on a complete sensor network graph, wherein the weight of each edge is the Euclidean distance between the constituent nodes of the edge. A Breadth-First-Search of the ud-MST, starting with the node located closest to the center of the network, is now conducted to iteratively construct a rooted directed minimum-weight spanning tree (rd-MST). The three tree traversal algorithms are then executed on the rd-MST and the node sequence resulting from each of the traversals is used as the chain of nodes for the PEGASIS protocol. Simulation studies on PEGASIS conducted for both TDMA and CDMA systems illustrate that using the chain of nodes generated from the tree traversal algorithms, the node lifetime can improve as large as by 19%-30% and at the same time, the energy loss per node can be 19%-35% lower than that obtained with the currently used distance-based greedy heuristic.

• Journal of KIISE
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• v.44 no.8
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• pp.842-853
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• 2017

Streaming data is a sequence of data samples that are consistently generated over time. The data distribution or concept can change over time, and this change becomes a factor to reduce the performance of a classification model. Adaptive incremental learning can maintain the classification performance by updating the current classification model with the weight adjusted according to the degree of concept drift. However, selecting the proper weight value depending on the degree of concept drift is difficult. In this paper, we propose a dynamic ensemble method based on adaptive weight adjustment according to the degree of concept drift. Experimental results demonstrate that the proposed method shows higher performance than the other compared methods.