• Title/Summary/Keyword: weight ratio

Search Result 7,960, Processing Time 0.036 seconds

Transfection Property of a New Cholesterol-Based Cationic Lipid Containing Tri-2-Hydroxyethylamine as Gene Delivery Vehicle

  • Kim, Bieong-Kil;Doh, Kyung-Oh;Hwang, Guen-Bae;Seu, Young-Bae
    • Journal of Microbiology and Biotechnology
    • /
    • v.22 no.6
    • /
    • pp.866-871
    • /
    • 2012
  • A novel cholesterol-based cationic lipid containing a tri-2-hydroxyethylamine head group and ether linker (Chol-THEA) was synthesized and examined as a potent gene delivery vehicle. In the preparation of cationic liposome, the addition of DOPE as helper lipid significantly increased the transfection efficiency. To find the optimum transfection efficiency, we screened various weight ratios of DOPE and liposome/DNA (N/P). The best transfection efficiency was found at the Chol-THEA:DOPE weight ratio of 1:1 and N/P weight ratio of 10~15. Most of the plasmid DNA was retarded by this liposome at the optimum N/P weight ratio of 10. The transfection efficiency of Chol-THEA liposome was compared with DOTAP, Lipofectamine, and DMRIE-C using the luciferase assay and GFP expression. Chol-THEA liposome with low toxicity had better or similar potency of gene delivery compared with commercial liposomes in COS-7, Huh-7, and MCF-7 cells. Therefore, Chol-THEA could be a useful non-viral vector for gene delivery.

A Study on the Relationship between Dietary Intakes and the Obesity of Middle School Studients in Seoul (서울시내 중학생들의 식품섭취실태와 비만과의 상관관계 연구)

  • 조주은;김주혜;송경희
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.23 no.1
    • /
    • pp.55-61
    • /
    • 1994
  • The study was designed to investigate the relationship between dietary intakes and the obesity of 400 students who were the third grade in middle school in some areas of Seoul. The survey conducted from March 3 to 13 , 1993. The results obtained were summarized as follows :The average height and weight of surveyed subject were similar to that of standard ( the third grade students in middle school in Seoul, 1991) . Compared to the standard weight/height ratio, subject who belonged to overweight and obese group were 16.4% of male students, 16.1% of female students respectively. Female subjects showed higher ratio than male subjects in skipping meals, frequency of snack per day and concern about a dieting . In case of subjects who belonged to weight /height ratio was high, their speed of dining was fast and they have more concern about a dieting. Compared to the amount of the nutritional intakes of RDA, calcium intakes of male and female subjects were deficient. Especially iron intakes of female subjects were deficient. In takes of Calorie (p<0.01) protein(p<0.05) and fat 9p<0.01) of obese group were higher those of non-obese group. Obese group showed good appetite and overeating also . Correlation analysis showed that obese group seemed to the closely associated with relative body weight of parents, family income, frequency of meals per day, frequency of meals per day, frequency of snack per day, speed of dining and overeating .

  • PDF

Pore properties and Microstructure on the each regions of a Light-Weight Aggregate using Glass Abrasive Sludge (유리연마슬러지를 사용한 경량골재의 미세구조 및 기공 특성)

  • Kwon, Choon-Woo;Chu, Yong-Sik;Kim, Young-Yup;Jung, Suk-Joe;Song, Hun;Lee, Jong-Kyu
    • Proceedings of the Korea Concrete Institute Conference
    • /
    • 2006.11a
    • /
    • pp.533-536
    • /
    • 2006
  • A light-weight aggregate with a surface layer was fabricated using glass abrasive sludge and expanding agents. The glass abrasive sludges were mixed with expanding agents ($Fe_2O_3,\;graphite,\;CaCO_3$) and formed into precursors. These precursors were sintered in the range of $700-900^{\circ}C$ for 20min. The sintered light-weight aggregate had a surface layer with smaller pores and an inner region with larger pores. The surface layer and pores controlled the water absorption ratio and physical properties. As the expanding agent fraction and the sintering temperature increased, the porosity and pore size increased. The light-weight aggregate with $Fe_2O_3$ and graphite as the expanding agents had a low water absorption ratio while the porous material with $CaCO_3$ as the expanding agent had a higher water absorption ratio and more open pores.

  • PDF

Utilization of Kenaf Cultivated in Korea(I) - Growth and Anatomical Characteristics of Kenaf Cultivated in Korea - (국내산 Kenaf 이용에 관한 연구(제1보) -국내에서 재배한 kenaf의 생장 및 해부학적 특성-)

  • 이명구;윤승락
    • Journal of Korea Technical Association of The Pulp and Paper Industry
    • /
    • v.35 no.4
    • /
    • pp.68-74
    • /
    • 2003
  • Kenaf(Hibiscus cannabinus L.) cultivar, Tainung 2, had been grown for 152 days at the experimental farm of Jinju National University, Gajoa-dong, Jinju-si, Kyongnam, Korea. The planting, growth rate, fertilization and structural characteristics as well as the cultivation and growth characteristics of kenaf, and the product usage were investigated. The narrowest diameter at kenaf bottom was 10 mm, the widest 42 mm and the average about 28 mm, and the shortest height 150 cm, the tallest 480 cm and the average about 350 cm. The weight of a core fraction was 68.1% and a bast fraction 31.9%. The weight ratio of core material to bast fiber was 2.31. The weight ratio of dry stem was 73.5% and that of leaves 26.5%. The weight of dry plant produced in 1 $m^2$ was 1,467 g, and about 1,052 g of stem could be used for the commercial purpose, The application of fertilizers resulted in the increase of the growth rate of the diameter at plant bottom and the height. Bast fiber, phloem ray and cortex parenchyma cell were observed in bast, and vessel, wood fiber and ray in core.

Effect of the Mixture Ratio of Ni-Pt Nanocatalysts on Water Electrolysis Characteristics in AEM System (Ni-Pt 나노 촉매의 혼합비가 음이온 교환막 수전해 특성에 미치는 영향)

  • LU, LIXIN;DAI, GUANXIA;LEE, JAEYOUNG;LEE, HONGKI
    • Transactions of the Korean hydrogen and new energy society
    • /
    • v.32 no.5
    • /
    • pp.285-292
    • /
    • 2021
  • To study the effect of the mixture ratio of Ni-Pt nanocatalysts on water electrolysis characteristics in anion exchange membrane system, Ni-Pt nanocatalysts were loaded on carbon black by using a spontaneous reduction reaction of acetylacetonate compounds. The loading weight of Ni-Pt nanocatalysts on the carbon black was measured by thermogravimetric analyzer and the elemental ratio of Ni and Pt was estimated by energy dispersive x-ray analyzer. It was found that the loading weight of Ni-Pt nanoparticles was 5.36-5.95 wt%, and the loading weight increased with increasing Pt wt%. As the Ni-Pt loading weight increased, the specific surface area decreased, because Ni-Pt nanoparticles block the pores of carbon black. It was confirmed by BET analysis and dynamic vapor sorption analysis. I-V characteristics were estimated.

Quantitative Growth Analysis of White and Reddish Sword Bean

  • Park, Sun-Young;Doo, Hong-Soo;Song, Seung-Kyoung;Ryu, Jeom-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.46 no.2
    • /
    • pp.89-94
    • /
    • 2001
  • Sword bean belongs to the subgenus Canavalia in the genus Canavalia. White and reddish varieties in sword bean were tested to obtain the basic information for improving the yield, quality, and cultural environments through the quantitative growth analysis. Growing plants were sampled at intervals of 10 days from 20 days after sowing. Whole plant weight increased during the period of 11-20 days after sowing in both varieties. The weight of each organ between two varieties increased similarly except leaf area. Whole plant weight changed more close to pod weight than any other characters. The leaf areas of both varieties increased from 50 days after sowing, var. white displayed S type curved line but var. reddish displayed slow S type curved line. The SGR of whole plant weight in both varieties had 3 maxima and 2 minima, 3 maxima and first minimum were shown at the same period but the second minimum was shown at different period. Both varieties showed bimodal curved line. All SGR of each organ and leaf area were shown the 2 maxima and 1 minimum in both varieties. The changes of SLA and LAR were not remarkable between two varieties but these were united together with the maximum of whole plant weight and root weight. ULWR and ULWR were shown similar in both varieties. Reddish variety was more prosperous in early growth stage than white variety because SLA, LAR and LWR were high. In surplus weight, both varieties increased rapidly during the period of 111-120 days after sowing that was applicable to the maximum SGR of pod weight. Surplus weight of var. white increased markedly during the same period comparing those of var. reddish.

  • PDF

The Effect of Samilshinkihwan on Immunosuppression Induced in Rats by Methotrexate (삼일신기환(三一腎氣丸)이 methotrexate로 유발(誘發)된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響))

  • Choi, Young-Ah;Kwon, Eun-Hee;Lee, Yeon-Kyeong;Shin, Hyeon-Cheol;Kang, Seok-Bong;Park, Song-Kee
    • The Journal of Internal Korean Medicine
    • /
    • v.28 no.1
    • /
    • pp.12-24
    • /
    • 2007
  • Objective : To investigate the effect of Samilshinkihwan(SISKW) on white rats with deteriorated immunity caused by methotrexate. Methods : The test articles were dosed once a day for 14 days by gastric gavage at a dosage 1000, 500 and 250mg/kg/10ml of SISKW from 2 days after last MTX-dosing, and changes in body weight, spleen weight, total blood leukocyte numbers, total lymphocyte numbers, B and T lymphocyte ratio, CD3+CD4+, CD3+/CD8+ and CD4+/CD8+ T lymphocyte ratio in the blood and spleen were measured. In addition, the serum interleukin (IL)-2 levels and the productivity of IL-2 of splenic cells were also demonstrated in this study. Results : The changes on body weight increased significantly in the 1000mg/kgof SISKW group. The changes on the spleen weight, the total blood leukocytenumbers, the total lymphocyte numbers in the blood and spleen, the ratio of T-cell in the blood and spleen and the ratio of CD3+CD4+ T-cell in spleen increased significantly in all SISKW groups as compared with the control group. The ratio of CD4+/CD8+ T-cells in blood increased significantly. The serum IL-2 levels and productivity of IL-2 of splenic cells increased significantly in 1000 and 500mg/kg SISKW groups as compared with the control group. Conclusions : Samilshinkihwanhas an effect of increasing immune responses, especially on cellular immune responses, in white rats with deteriorated immunity caused by methotrexate.

  • PDF

Low Back Pain and Related Factors in Men Workers for Manufacturing Industry (일부 제조업 남성근로자의 요통경험 및 관련요인)

  • Yi Seung-Ju;Kim Ki-Yeol;Cha Sang-Eun;Park Sang-Rae;Lim Won-Sik
    • The Journal of Korean Physical Therapy
    • /
    • v.12 no.2
    • /
    • pp.7-15
    • /
    • 2000
  • Objectives: The purpose of this study w8s to investigate the experience rate and point prevalence and factors related with Low Back Pain(LBP) in men workers for the manufacturing industry. Methods: Questionnaires were completed by 97 men workers for 1 vehicle company in Taegu city in September 6-26, 2000. The information was used to estimate odds ratio and $95\%$ confidence intervals fur the LBP related factors association. Results: The experience rate for LBP was $67.0\%$. point prevalence was $14.4\%$. Variables significantly associated with LBP were weight(p=0.052) and smoking(p=0.010). $57\%$ for patients with LBP was higher than 50.095 far without in normal weight. whereas $43.0\%$ for patients with LBP was smaller than $50.0\%$ for without in overweight. $73.8\%$ for patients with smoking increased than $46.9\%$ for smokers without in smoking. The experience for LBP increased as weight increased(Odds ratio=2.923). As subjects are getting older. experience increased(Odds ratio= 1.393). The higher subjects had experienced stress, the higher experience for LBP was(Odds ratio= 1.328), however all three variables had no significant relationship. Conclusions: Results from this study indicated that a statistically significant association between LBP and weight. smoking in X2-test. In logistic regression test, there were no related variables.

  • PDF

Effects of Alternate-Week Feeding Strategies on Growth and Feed Efficiency Ratio of Juvenile Nile Tilapia Oreochromis niloticus in a Recirculating System

  • Cho, Sung-Hwoan
    • Fisheries and Aquatic Sciences
    • /
    • v.8 no.3
    • /
    • pp.128-131
    • /
    • 2005
  • The effects of alternate-week feeding strategies on growth and feed efficiency ratio of juvenile Nile tilapia were investigated in a recirculating aquaculture system. Twenty fish initially weighing 25.5 g were randomly distributed into each of 18 tanks to conduct three replicates for each of six different feeding strategies. Weight gain of fish in the control that were fed daily for 6-week feeding trial was significantly higher than those of fish subjected to various alternate-week feeding regimes. Weight gain of fish that were starved for 3 weeks and then fed daily for 3 weeks (3WS+3WF) was not significantly different from that of fish, starved and fed daily for alternate 1-week period during the trial (1WS+1WF), but was significantly higher than those of fish starved for 2 weeks, and fed for 2 weeks (2WS+2WF); fish starved for 4 weeks and fed for 2 weeks (4WS+2WF); and fish fed for 5 weeks and fed for 1 week (5WS+1WF.) The amount of feed supplied to fish in the groups of 1WS+1WF and 3WS+3WF was significantly lower than that fed to fish in the control group, but significantly higher than the amount feed supplied to fish in the other three groups. Feed efficiency ratio for fish in the control group did not differ from that for fish in the groups of 1WS+1WF, 2WS+2WF and 3WS+3WF, but was significantly higher than that for fish in the groups of 4WS+2WF and 5WS+1WF. In conclusion, juvenile Nile tilapia that were subject to starvation for 1 to 5 weeks did not exhibit compensatory growth sufficient to attain the same weight as fish fed daily for 6 weeks. In addition, fish subjected to starvation exhibited low feed efficiency ratio compared to fish fed daily, which were probably attributable to poor weight gain.

PLASTIC STRAIN RATIOS AND PLANAR ANIOSOTROPY OF AA5182/POLYPROPYLENE/AA5182 SANDWICH SHEETS

  • KIM K. J.
    • International Journal of Automotive Technology
    • /
    • v.6 no.3
    • /
    • pp.259-268
    • /
    • 2005
  • In order to analyze the sheet drawability, the measurement of the plastic strain ratio was carried out for the 5182 aluminum alloy sheets in which were cold rolled without lubrication and subsequent recrystallization annealing. The average plastic strain ratio of the 5182 aluminum sheets was 1.50. It was considered that the higher plastic strain ratio was resulted from the ND//<111> component evolved during rolling and maintained during annealing. The AA5182/polypropylene/AA5182 (AA/PP/AA) sandwich sheets of the 5182 aluminum alloy skin sheet and the polypropylene core sheet with high formability have been developed for application for automotive body panels in future light weight vehicles with significant weight reduction. The AA/PP/AA sandwich sheets were fabricated by the adhesion of the core sheet and the upper and lower skin sheets. The AA/PP/AA sandwich sheet had high plastic strain ratio (1.58), however, the planar anisotropy of the sandwich sheet was little changed after fabrication. The optimum combination of directionality of the upper and lower skin sheets having high plastic strain ratio and low planar anisotropy was calculated theoretically and an advanced process for producing the sandwich sheets with high plastic strain ratio was proposed. The developed sandwich sheets have a high average plastic strain ratio of 1.55 and a low planar anisotropy of 0.17, which was improved more by 3.2 times than that of 5182 aluminum single sheet.