• Korean Journal of Weed Science
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• v.31 no.4
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• pp.375-383
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• 2011

This experiment was carried out to confirm characteristics of macro granule (GG) for herbicidal efficacy by using remote-controlled aerial application (RCAA) to control annual and perennial weeds in rice paddy field, and phytotoxicity to rice and non target plants of formulation types. Herbicidal efficacy of GG applied by using RCAA was 93.6~96.6% in flooded direct sowing cultivation. There was no difference in phytotoxicity between the hand and the power applicator methods in terms of height, number of tiller and yield component. Herbicidal efficacy of GG applied by using RCAA was 92.7~97.3% in machine transplanting rice field. There was no difference in phytotoxicity between the hand and RCAA methods in terms of height, number of tiller and yield component. When suspension concentrate (SC) and GG were applied directly on Chinese cabbage, lettuce, cucumber, pepper, soybean and wild sesame, several symptoms of injury such as, the inhibition of growth, yellowish, leaf fall and withering was observed.

• Journal of the Korean Institute of Landscape Architecture
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• v.45 no.5
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• pp.42-59
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• 2017

To investigate rooting and community forming ability, sod strips of Gramineae such as Miscanthus sacchariflorus (Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg., and Arundinella hirta(Thunb.) Tanaka were planted horizontally at a width of 10~15cm in October 2010 on the bank slope of Kwangju Stream in Korea. Seeds of each species were sown in April 2010 in plastic seedbeds(60cm length ${\times}$ 30cm width ${\times}$ 5cm depth) filled with 4cm of garden soil and grew until October 2010 to form rectangular sod, which then was cut into multiple strips(20cm length ${\times}$ 6cm width ${\times}$ 4cm root depth). Weeds growing on the experiment sites were removed twice a year until those four species had formed a community by 2013. Weeds were not eradicated during 2014 and 2015 to observe whether the community formation can be maintained without weed removal. t-tests on stem numbers and heights in May, July, and September were conducted between 2013 and 2014, and between 2014 and 2015. Stem numbers and heights of the four species in 2014 were significantly decreased(p<0.001) compared with those in 2013 due to weed damage to their growth in 2014. Stem numbers and heights of Miscanthus sacchariflorus(Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg. in 2015 were similar to those in 2014(p>0.05). Stem numbers and heights of Arundinella hirta(Thunb.) Tanaka in 2015, however, were significantly reduced compared with those in 2014(p<0.001) due to weed impact on its growth. After three years of weed removal, a community of each species was formed. Miscanthus sacchariflorus(Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg. were able to establish communities without any further weed removal. Arundinella hirta(Thunb.) Tanaka, however, was not able to maintain its community due to considerable damage to its growth caused by weeds even after three years of weed removal. Miscanthus sacchariflorus(Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg. are more suitable to sod strip plantings on stream bank slopes in terms of the maintenance of community formation without weed eradication. t-test on stem numbers and heights each September during the five year experiment period between sod strip planting and potted plants of the four species were conducted. Stem numbers of strip sod plantings were significantly higher(p<0.001) than those of potted one. Heights of strip sod plantings, however, were significantly lower(p<0.05) than those of the potted samples. Therefore, strip sod planting is more advantageous regarding bank slope erosion protection due to the higher number of stems.

• Korean Journal of Weed Science
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• v.15 no.4
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• pp.289-297
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• 1995

Field experiments were conducted to evaluate the competitive characteristics of weeds with direct-seeded rice. In both dry- and water-seeded rice a weeds) started to grow over the plant height of rice from 45 days after seeding, and there was a great difference in plant height between rice and weeds from 75 days after seeding. Dry weights of weeds drastically increased from 30 days after seeding in both dry- and water-seeded rice, and the increase of dry weight was faster in dry-seeded rice than in water-seeded rice with time. Although weeds competed with rice at earlier growth stages, they did not reduce rice yields and its components until competition period of 75 days after seeding. Required period of weed free at the beginning of the season was 30 to 45 days from seeding in order to avoid any yield loss. The numbers of panicle per plant, spikelets per panicle, grain weight and yield of rice in dry-seeded rice had highly negative correlations with the growth ratios of Echinochloa crus-galli, Ludwigia prostrata, Cyperus difformis, Bidens frondosa, and Cyperus serotinus. In water-seeded rice, the number of plants, panicle per plant, spikelets per panicle and yield had highly negative correlations with growth rate of Echinochloa crus-galli, but the percentage of filled spikelet had negative correlation with Cyperus difformis. Research indicated that in direct-seeded rice weed competition with rice started from 30 days after seeding and significant yield loss by weed competition occurred from 75 days after seeding.

• Research in Plant Disease
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• v.14 no.1
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• pp.15-20
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• 2008

Three isolates of Cucumber mosaic virus (CMV) were isolated from weed hosts showing typical mosaic symptoms, and some properties of the viruses were investigated. CMV isolates, designated as Is-CMV, Jd-CMV and Pla-CMV from Isodon inflexus, Jeffersonia dubia and Phryma leptostachya var. asiatica, respectively, were identified and characterized by biological reaction in several host plants, serological property, dsRNA analysis, reverse transcription-polymerase chain reaction (RT-PCR), restriction fragment-length polymorphism (RFLP). All isolates systemically infected in Nicotiana benthamiana, Cucurbita pepo cv. Black beauty and Cucumis sativus, and did not reveal any differences in these host plants between the isolates. However, remarkable difference in the symptoms was found between the CMVs in Capsicum annuum. Is-CMV induced an asymptomatic symptoms, while Jd-CMV and Pla-CMV produced severe mosaic symptoms in C. annuum plants. In dsRNA analysis, all isolates revealed four major bands with estimated molecular size of 3.4, 3.2, 2.1 and 1.0 kbp. The cDNAs of coat protein gene of the isolates were amplified by RT-PCR using a genus-specific single pair primers that designed to amplify a DNA fragment of approximately ranging from 938 to 966 bp. By restriction mapping analysis using RFLP of the RT-PCR products as well as by serological properties of gel diffusion test, the CMV isolates belong to a typical members of CMV subgroup IA. This is the first report on the occurrence of CMV in the three weed hosts.

• Korean Journal of Weed Science
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• v.30 no.4
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• pp.396-404
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• 2010

Phenolic compounds, which are products of secondary metabolism, have been demonstrated to be widespread growth substances in plants. The objectives of this study were to identify the phenolic compounds in common ragweed (Ambrosia artemisiifolia var. elatior) by HPLC and to evaluate their effects on germination and seedling growth of three weed species. Under controlled conditions in Petri dishes at $25^{\circ}C$, $10^{-3}$ and $10^{-4}$ M solutions of phenolic compounds were evaluated in seed germination tests. Four phenolic compounds (caffeic acid, O-coumaric acid, ${\rho}$-coumaric acid and ferulic acid) in common ragweed plant were identified and their concentration was increased from the stage before flowering through full flowering stage. Treatment of O- and ${\rho}$-coumaric acids delayed the seed germination of Digitalia ciliaris, while the treatment of caffeic acid delayed the seed germination of Echinochloa crus-galli. In time to 50% germination ($T_{50}$), phenolic compounds at $10^{-4}$ M promoted in Cyperus microiria and E. crus-galli but the level of $10^{-3}$ M delayed the $T_{50}$ of those weeds. The O-coumaric acid inhibited seed germination and seedling growth of the tested weeds and especially it perfectly inhibited the root growth of E. crus-galli.

• Korean Journal of Weed Science
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• v.15 no.4
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• pp.321-328
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• 1995

Absorption, translocation, and metabolism study of $^{14}C$-bensulfuron were conducted to determine selectivity and herbicidal action of bensulfuron in two rice cultivars and three paddy weeds at different temperatures. 1. Absorption of $^{14}C$-bensulfuron was greater at 30/$25^{\circ}C$ than at 25/$20^{\circ}C$ and also in cv. Sangpung than in cv. Samgang, and Cyperus serotinus and Sagittaria pygmaea showed greater amount of absorption than Echinochloa crus-galli. 2. Translocation rate of bensulfuron was higher at 30/$25^{\circ}C$ than at 25/$20^{\circ}C$ and also in cv. Sangpung than in cv. Samgang, and C. serotinus showed highest translocation rate followed by the S. pygmaea and E. crus-galli. 3. In metabolism study, concentration of parent compound in rice plants was greater in cv. Sangpung which was susceptible to bensulfuron than in cv. Samgang. More amount of parent compound was distributed in shoots and root of C. serotinus and S. pygmaea than E. crus-galli. 4. It may suggested that sensitivity to bensulfuron between rice cultivars may be due to different inactivation metabolic ability and phytotoxicity of rice increased at high temperature since higher amount of bensulfuron was absorbed. Higher herbicidal activity of bensulfuron may caused by higher absorption and translocation in three weed species at high temperature.

• Korean Journal of Weed Science
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• v.7 no.3
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• pp.289-298
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• 1987

This study was conducted to investigate the presence of biologically active substances such as phenolic acids, fatty acids and organic acids in the medical plants like Kalopanax pictum and Acanthopanacis cortex. Alcohol extracts of K. pictum and A. cortex showed complete inhibition of lettuce seed germination, indicating that these plants contained the biologically active substances. Eleven phenolic acids including protocatechuic acid were identified from K. pictum and A. cortex by GLC, and the contents of total phenolic acid were 1.7917mg/g in K. pictum, and 0.9567mg/g in A. cortex. Polyphenols such as neochlorogenic acid, chi orogenic acid, scopoletin, rutin and kaempferolglycoside which were not detected by GLC were anayzed by HPLC, and among phenolic acids indentified chi orogenic acid seemed to be the major acid in both K. pictum and A. cortex presented in amount of 23.7 and 13.0ppm, respectively. K. pictum contained 5.26mg/g of fatty acids and 27.69mg/g of organic acids, and A. cortex possessed 3.22mg/g of fatty acids and 9.80mg/g of organic acids, linoleic and oxalic acid appeared to be the major fatty and organic acids, representing more than 50% of total fatty acids and 80% of total organic acids.

• Korean Journal of Weed Science
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• v.6 no.1
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• pp.48-58
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• 1986

In order to eluciate the factors affecting the injury caused by simetryne(2-methyl thio-4,6-bis(ethylamino)-S-triazine) to rice plants of Tongil variety(Indica ${\times}$ Japonica), the leaching properties of simetrynes, compared with Japonica varieties, Mangyeung and Sadominori were investigated. Bioassay technique and $^{14}C$-simetryne for leaching study and bioassay for the characteristics of the rice plants were employed. The leaching range of simetryne was very narrow regardless of soil types under upland soil conditions but it increased little in Silt clay soil and greatly in Sandy loam soil under flooded soil conditions. The amount of the roots of Tongil Jas a little more than that of Mangyeung and the roots of the Tongil mostly distributed near the surface soil while those of Mangyeung rooted deep into the soil. The foliage area and the rate of transpiration of Tongil were larger and greater than those of Mangyeung by 1.3 times and 1.65 times, respectively. The sensitivity of Tongil to simetryne was about 3 times larger than that of Sadominori in water culture.

• Korean Journal of Weed Science
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• v.5 no.2
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• pp.131-136
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• 1985

This experiment was conducted to identify the competitive ability of rice against Monochoria vaginalis Presl. and to determine effect of various herbicides on it. Photosynthetic efficiency of rice markedly increased as the density of M. vaginalis increased from 1 to 3 plants per hill. Competition index increased in propotional to an increase of M. vaginalis density while total dry matter of rice decreased in reverse. Significant yield reduction of rice, was observed at the density of M. vaginalis, 2 plants per hill and 37% at 3 plants per hill. Such a yield reduction can be mainly attributed to the decrease of panicle and spikelet number of rice which were greatly affected by competition with M. vaginalis. Regardless of herbicides tested, % inhibition increased remarkably as the concentration of herbicides increased from 1 to 20 ppm. No plant growth was observed in all the herbicides treated with 20 ppm except for butachlor, thiobencarb and bifenox, showing existence of the new promising herbicides to control M. vaginalis among the herbicides tested.

• Korean Journal of Weed Science
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• v.16 no.2
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• pp.122-131
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• 1996

The inhibition characteristics of chlorsulfuron [CHL, 2-chloro-N-[{ (4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino}carbonyl]benzenesulfonamide] and imazaquin [IMA, 2-{4,5-dihydro-4-methyl-4-(1-methy-lethyl)-5-oxo-1H-imidazol-2-yl}-3-quinolinecarboxylic acid] on acetolactate synthase(ALS) activity of corn plants were investigated. CHL and IMA rapidly inhibited ALS activity of corn plants in vitro. Their $I_{50}$ values for ALS activity were 100nM and $5{\mu}M$, respectively, indicating that CHL had 50 times more inhibitory effect on ALS activity than IMA. The first applied herbicide had a dominant inhibitory effect on ALS activity when the two herbicides were applied sequentially. Branched-chain amino acids, valine(Val), leucine(Leu), and isoleucine(Ile) showed a feedback inhibition on ALS activity ; Val or Leu had a more inhibitory effect on ALS activity than Ile. Branchedchain amino acids and CHL or IMA exhibited an additive effect on inhibiting ALS activity. This suggests that branched-chain amino acids inhibit ALS activity by a different mechanisms) from that of CHL or IMA. Apparent ALS activity, which was measured on the basis of the conversion of pyruvate to acetolactate, was decreased by the addition of 2-ketobutyrate into the ALS reaction mixture in a concentration-dependent manner. In addition, kinetic studies revealed that CHL acts as a noncompetitive inhibitor, while IMA acts as an uncompetitive inhibitor to ALS with respect to pyruvate.