• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.21 no.3
• /
• pp.91-98
• /
• 2021

In most hacking attacks, hackers tend to access target systems in a variety of circumvent connection methods to hide their original IP. Therefore, finding the attacker's IP(Internet Protocol) from the defender's point of view is one of important issue to recognize hackers. If an attacker uses a proxy, original IP can be obtained through a program other than web browser in attacker's computer. Unfortunately, this method has no effect on the connection through VPN(Virtual Private Network), because VPN affects all applications. In an academic domain, various IP traceback methods using network equipments such as routers have been studied, but it is very difficult to be realized due to various problems including standardization and privacy. To overcome this limitation, this paper proposes a practical way to use client's network configuration temporarily until it can detect original IP. The proposed method does not only restrict usage of network, but also does not violate any privacy. We implemented and verified the proposed method in real internet with various VPN tools.

• Entomological Research
• /
• v.48 no.5
• /
• pp.390-399
• /
• 2018

In order to precisely assess gene expression levels, the suitable internal reference genes must be served to quantify real-time reverse transcription polymerase chain reaction (RT-qPCR) data. For armyworm, Mythimna separata, which reference genes are suitable for assessing the level of transcriptional expression of target genes have yet to be explored. In this study, eight common reference genes, including ${\beta}$-actin (${\beta}$-ACT), 18 s ribosomal (18S), 28S ribosomal (28S), glyceraldehyde-3-phosphate (GAPDH), elongation fator-alpha ($EF1{\alpha}$), TATA box binding protein (TBP), ribosomal protein L7 (RPL7), and alpha-tubulin (${\alpha}$-TUB) that in different developmental stages, tissues and insecticide treatments of M. separata were evaluated. To further explore whether these genes were suitable to serve as endogenous controls, three software-based approaches (geNorm, BestKeeper, and NormFinder), the delta Ct method, and one web-based comprehensive tool (RefFinder) were employed to analyze and rank the tested genes. The optimal number of reference genes was determined using the geNorm program, and the suitability of particular reference genes was empirically validated according to normalized HSP70, and MsepCYP321A10 gene expression data. We found that the most suitable reference genes for the different experimental conditions. For developmental stages, 28S/RPL7 were the optimal reference genes, both $RPL7/EF1{\alpha}$ were suitable for experiments of different tissues, whereas for insecticide treatments, $28S/{\alpha}-TUB$ were suitable for normalizations of expression data. In addition, $28S/{\alpha}-TUB$ were the suitable reference genes because they have the most stable expression among different developmental stages, tissues and insecticide treatments. Our work is the first report on reference gene selection in M. separata, and might serve as a precedent for future gene expression studies.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.22 no.2
• /
• pp.379-390
• /
• 2012

This study is to consider political implication of indicators to measure personal information security in public sector studied by Ministry of Public Adminstration and Security from 2008 to 2011. The study analyzed the priority of personal information security policy dividing into personal information security infrastructure, personal information management with life cycle, correspondence of information infringement by scholars, experts, and chargers. As the results, to progress personal information security policy is important to management of personal identification information on web site; specially institutional infrastructure as responsible organization, exclusive manpower, and security budget; personal information security infrastructure. As like the results, it would be reflected in the progress of personal information security policy and tried to provide systematic management program with improving safe information distribution and usefulness.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.6
• /
• pp.882-889
• /
• 2021

In order to use an enzyme industrially, it is necessary to increase the activity of the enzyme and optimize the reaction characteristics through molecular evolution techniques. We used the error-prone PCR method to improve the reaction characteristics of LipCA lipase discovered in Antarctic Croceibacter atlanticus. Recombinant Escherichia coli colonies showing large halo zones were selected in tributyrin-containing medium. The lipase activity of one mutant strain (M3-1) was significantly increased, compared to the wild-type (WT) strain. M3-1 strain produced about three times more lipase enzyme than did WT strain. After confirming the nucleotide sequence of the M3-1 gene to be different from that of the WT gene by four bases (73, 381, 756, and 822), the secondary structures of WT and M3-1 mRNA were predicted and compared by RNAfold web program. Compared to the mean free energy (MFE) of WT mRNA, that of M3-1 mRNA was lowered by 4.4 kcal/mol, and the MFE value was significantly lowered by mutations of bases 73 and 756. Site-directed mutagenesis was performed to find out which of the four base mutations actually affected the enzyme expression level. Among them, one mutant enzyme production decreased as WT enzyme production when the base 73 was changed (T→ C). These results show that one base change at position 73 can significantly affect protein expression level, and demonstrate that changing the mRNA sequence can increase the stability of mRNA, and can increase the production of foreign protein in E. coli.

• Journal of The Korean Society of Integrative Medicine
• /
• v.9 no.1
• /
• pp.59-67
• /
• 2021

Purpose : This study examined the factors related to sexual intercourse among adolescents in South Korea. Methods : The study sample was comprised of 65,528 middle and high school students aged 12 to 18 years in 2016. For this study, using the data from the 12th Korea youth risk behavior web-based survey, a chi-squared test, and hierarchical logistic regression analysis we reconducted with the SPSS 22.0 program considering personal, family, school, and regional characteristics. Results : The proportion of adolescents who had sexual intercourse was 6.0 % of boys and 2.7 % of girls, and 6.3 % of high school students and 2.4 % of middle school students. The statistically significant related factors of sexual intercourse were gender, grade, attempted suicide, ever drinking, ever smoking, habitual or purposeful drug use, weekly allowance, and coed school in the final model 4. Adolescents with suicide attempts had 2.49 times more sexual intercourse than adolescents with no experience of suicide attempts. Compared to adolescents without alcohol, smoking, and habitual drug use, those who have experienced alcohol, smoking, habitual or purposeful drug use were 2.22 times, 3.76 times, and 3.39 times more likely to have had sexual intercourse, respectively. Adolescents with a weekly allowance of more than 100,000 won per week and adolescents in coed schools were 2.84 times and 1.40 times more likely to have had sexual intercourse, respectively. Conclusion : Multi-level interventions should be considered in school sexual education programs while considering the substantiality of sex education and the actual adolescents' needs. In addition, sexual health interventions in cyberspace for high-risk groups may be needed to consider the emotional and mental characteristics, given the factors related to sexual intercourse in adolescents.

• The Journal of the Convergence on Culture Technology
• /
• v.8 no.4
• /
• pp.375-380
• /
• 2022

The purpose of this study is to find out consumers' perception and meaning of convenience store convenience food by using big data. For this study, NNAVER and Daum analyzed news, intellectuals, blogs, cafes, intellectuals(tips), and web documents, and used 'convenience store convenience food' as keywords for data search. The data analysis period was selected as 3 years from January 1, 2019 to December 31, 2021. For data collection and analysis, frequency and matrix data were extracted using TEXTOM, and network analysis and visualization analysis were conducted using the NetDraw function of the UCINET 6 program. As a result, convenience store convenience foods were clustered into health, diversity, convenience, and economy according to consumers' selection attributes. It is expected to be the basis for the development of a new convenience menu that pursues convenience and convenience based on consumers' meaning of convenience store convenience foods such as appropriate prices, discount coupons, and events.

• Journal of Industrial Convergence
• /
• v.20 no.1
• /
• pp.87-96
• /
• 2022

This study aims to understand the actual conditions of adolescents with allergic diseases and factors related to mental health. It is intended to provide basic data for disease management. As for the research method, big data of the 16th Youth Health Behavior Survey were analyzed secondary, and x²-test was used using the SPSS 25 program8, odds ratio and 95% confidence interval were calculated to determine the degree of association, and multi-logistic regression analysis was performed. Studies have shown that 37.1% of adolescents with allergic diseases have stress perception, 27.6% of depression experience rate and 12.5% of suicidal thoughts, and the likelihood of stress perception, depression experience, and suicidal thoughts among adolescents with allergic diseases was higher. As a result of this study, it was found that the presence or absence of allergic diseases in adolescents has a significant effect on mental health, and we intend to use it as basic data for prevention and research on diseases that can lead to mental illness and adolescent mental health.

• Microbiology and Biotechnology Letters
• /
• v.49 no.1
• /
• pp.65-74
• /
• 2021

Serine proteases are the most versatile proteolytic enzymes with tremendous applications in various industrial processes. This study was designed to investigate the biochemical properties, critical residues, and the catalytic potential of alkaline serine protease using in-silico approaches. The primary sequence was analyzed using ProtParam, SignalP, and Phyre2 tools to investigate biochemical properties, signal peptide, and secondary structure, respectively. The three-dimensional structure of the enzyme was modeled using the MODELLER program present in Discovery Studio followed by Molecular Dynamics simulation using GROMACS 5.0.7 package with CHARMM36m force field. The proteolytic potential was measured by performing docking with casein- and keratin-enriched residues, while the effect of the inhibitor was studied using phenylmethylsulfonyl fluoride, (PMSF) applying GOLDv5.2.2. Molecular weight, instability index, aliphatic index, and isoelectric point for serine protease were 39.53 kDa, 27.79, 82.20 and 8.91, respectively. The best model was selected based on the lowest MOLPDF score (1382.82) and DOPE score (-29984.07). The analysis using ProSA-web revealed a Z-score of -9.7, whereas 88.86% of the residues occupied the most favored region in the Ramachandran plot. Ser327, Asp138, Asn261, and Thr326 were found as critical residues involved in ligand binding and execution of biocatalysis. Our findings suggest that bioengineering of these critical residues may enhance the catalytic potential of this enzyme.

• Journal of The Korean Society of Integrative Medicine
• /
• v.10 no.2
• /
• pp.61-72
• /
• 2022

Purpose : This study aimed to identify the educational effects of the International Development Cooperation and Healthcare course from 2018 to 2020. Methods : Changes in awareness of international development cooperation (understanding international development cooperation as well as government Official Development Assistance [ODA], thoughts on aid, and thoughts on government's aid scale) were analyzed using web-based surveys. The pre-course survey was conducted 1-2 weeks before the semester started, and the post-course survey was conducted 1-2 weeks after the semester ended. All data were analyzed using the SPSS 26.0 program, and statistical significance was set at p< .05. Results : A total of 314 people participated in the survey before taking the course, and 286 people participated in the survey after taking the course. Analysis of the changes in students' awareness of international development cooperation showed that all items (understanding international development cooperation and government ODA, as well as having thoughts on aid and government's aid scale) were improved. Regarding changes in awareness according to general characteristics, thoughts on aid were statistically significant according to all characteristics except for 2018. Regarding government's aid scale, awareness scores increased after taking the course in students who majored in healthcare, fine arts and athletics, broadcasting and media studies, and digital contents. Conclusion : This study showed that changes in awareness of international development cooperation were largest in second-year students. Compared to changes regarding international development cooperation, government ODA, and thoughts on aid, changes regarding thoughts on government's aid scale were minor. In the future, it is necessary to develop class content suitable for the first-year level and to supplement and operate content that can change the way of thinking on government's aid scale.

• Journal of the Korean BIBLIA Society for library and Information Science
• /
• v.33 no.4
• /
• pp.27-47
• /
• 2022

The purpose of the study is to develop a unit catalog system that can be linked with the curriculum. As for the research method, first, unit subject words were extracted through the analysis of middle school curriculum and science textbooks in all grades. Based on this, we designed a unit catalog system that has a unit book catalog by connecting books related to the unit topic of the subject, an index word catalog that organizes unit subject words into index words, and a grade and school level linked subject linked to the unit topic as a subsystem. Using the Notion program, a unit catalog system was implemented on the web using a middle school science unit 1 as a sample. To evaluate the system, experiments and surveys were conducted targeting students, and very positive results were shown in terms of satisfaction with the system and intention to use it in the future. Meanwhile, this study was limited to middle school science departments, but it was proposed to expand the application to all grades and subjects.