• Asian-Australasian Journal of Animal Sciences
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• 제20권10호
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• pp.1473-1477
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• 2007

Four-and-a-half LIM-only protein 3 (FHL3) is a member of the LIM protein superfamily and can participate in mediating protein-protein interaction by binding one another through their LIM domains. In this study, the 5'- and 3'- cDNA ends were characterized by RACE (Rapid Amplification of the cDNA Ends) methodology in combination with in silico cloning based on the partial cDNA sequence obtained. Bioinformatics analysis showed FHL3 protein contained four LIM domains and four LIM zinc-binding domains. In silico mapping assigned this gene to the gene cluster MTF1-INPP5B-SF3A3-FHL3-CGI-94 on pig chromosome 6 where several QTL affecting intramuscular fat and eye muscle area had previously been identified. Transcription of the FHL3 gene was detected in spleen, liver, kidney, small intestine, skeletal muscle, fat and stomach, with the greatest expression in skeletal muscle. The A/G polymorphism in exon II was significantly associated with birth weight, average daily gain before weaning, drip loss rate, water holding capacity and intramuscular fat in a Landrace-derived pig population. Together, the present study provided the useful information for further studies to determine the roles of FHL3 gene in the regulation of skeletal muscle cell growth and differentiation in pigs.

• Korean Journal of Human Ecology
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• 제24권1호
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• pp.123-135
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• 2015

In order to examine allergic disease experience rates of students at atopy and asthma friendly elementary schools located in Chungcheongnam-do and investigate their dietary behaviors, a survey on 250 parents of first to third grade students at four rural-type atopy and asthma friendly elementary schools was conducted. The number of students who had experienced allergic symptoms (allergic group) was 102 (40.8%). their breastfeeding duration was shorter than that of the non-allergic group (p<.001) and the former's weaning foods-feeding had started earlier than the latter's (p<.01). Regarding the eating habits, the non-allergic group was found to have breakfast and dinner better than the allergic group (p<.001). The number of students eating fruits as snacks was largest in the allergic group at 73 (71.6%), and as for the non-allergic group, their rate of eating fruits, milk and dairy products, and cookies and fries was at 25.7%, 22.3%, and 20.3%, respectively. A high percentage of the allergic group ate three times or less per month the animal food groups and dairy products (p<.001). Accordingly, for improvement in dietary behaviors of students who have allergy against food, nutrition education on regular eating, balanced intake of food, adequate intake of snacks and meals for proper growth and development and precise diagnosis of allergy-triggering food are necessary. In particular, as education on food allergy, implementation of practical education such as appropriate nutrition counseling by nutrition teachers is urgent beyond the one-way educational method of school newsletters.

• Journal of Chest Surgery
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• 제49권3호
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• pp.151-156
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• 2016

Background: Survival of children experiencing cardiac arrest refractory to conventional cardiopulmonary resuscitation (CPR) is very poor. We sought to examine current era outcomes of extracorporeal CPR (ECPR) support for refractory arrest. Methods: Patients who were <18 years and underwent ECPR between November 2013 and January 2016 were including in this study. We retrospectively investigated patient medical records. Results: Twelve children, median age 6.6 months (range, 1 day to 11.7 years), required ECPR. patients' diseases spanned several categories: congenital heart disease (n=5), myocarditis (n=2), respiratory failure (n=2), septic shock (n=1), trauma (n=1), and post-cardiotomy arrest (n=1). Cannulation sites included the neck (n=8), chest (n=3), and neck to chest conversion (n=1). Median duration of extracorporeal membrane oxygenation was five days (range, 0 to 14 days). Extracorporeal membrane oxygenation was successfully discontinued in 10 (83.3%) patients. Nine patients (75%) survived more than seven days after support discontinuation and four patients (33.3%) survived and were discharged. Causes of death included ischemic brain injury (n=4), sepsis (n=3), and gastrointestinal bleeding (n=1). Conclusion: ECPR plays a valuable role in children experiencing refractory cardiac arrest. The weaning rate is acceptable; however, survival is related to other organ dysfunction and the severity of ischemic brain injury. ECPR prior to the emergence of end-organ injury and prevention of neurologic injury might enhance survival.

• Asian-Australasian Journal of Animal Sciences
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• 제15권4호
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• pp.565-569
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• 2002

Thirty six young New Zealand white rabbits were used in a randomised complete block (RCB) design with a $3{\times}2$ factorial treatment experiment to study the suitability of sorghum as substitute for maize in the diet of growing rabbits in Kenya. Six different diets were formulated to contain 35% of one of the three different types of grain (maize, white sorghum or brown sorghum) and one of the two different levels of crude protein (CP) 16 or 18.5% and fed to growing rabbits for a period of six weeks. The tannin content of the grains was 0.05, 0.52 and 5.6% chatechin equivalents for maize, white and brown sorghum respectively. Weaning weight at 35 days of age was used as the blocking criterion at the beginning of the experiment. Results of feed intake, weight gain, feed conversion efficiency, feed digestibility, as well as the blood parameters, indicated that white sorghum was not significantly different from maize. Animals fed on diets containing brown sorghum had a lower average daily gain (ADG) and a poorer feed conversion efficiency (FCE) (p<0.01) in comparison with those fed on diets containing maize or white sorghum. The 18.5% CP level gave a better FCE (p<0.05) compared with the 16% CP level. However, increasing the level of CP did not improve the utilisation of any of the grains. It was concluded that white sorghum could effectively substitute maize in the diet of growing rabbits. On the other hand, the use of brown sorghum in the diets of growing rabbits may compromise their growth rate. This may be due to the high concentration of tannins in the brown sorghum.

• Asian-Australasian Journal of Animal Sciences
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• 제4권4호
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• pp.333-339
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• 1991

Eighty-two ewes of Balochi breed, two to four years of age were used in a completely randomized design to study the effect of winter supplementation on their performance in Kalat area of Balochistan and randomly divided into two groups of 40 and 42 animals. Two treatments (T1 and T2) studied were : 250 gm/animal/day of a 50 : 50 mixture of cottonseed cake and barley grain fed from Oct. 20 to Dec. 18, 1988 plus grazing and 500 gm/animal/day of the same feed mixture fed from Oct. 9 to Dec. 18, 1988 in addition to grazing. Lucerne hay and wheat straw in a 50 : 50 ratio were provided to all the ewes for a period of one month from Jan. 6, 1989 @ 320 gm/animal/day to sustain them in severe winter. Same feeding levels to the same ewe groups were again fed from Mar. 1 to May 27, 1989. Three breeding rams stayed with the flock from Nov. 1 to Dec. 13, 1988. Lambing took place from Apr. 2 to May 12, 1989. Conception, lambing and mortality percentage was found different (P<.05) between T1 and T2 (12.5 vs 14.8 kg). The ewes on T2 maintained higher body weights throughout winter than the ewes on T1. The results are suggestive of improvement in conception rate with winter supplementation (flushing) and decrease in ewe mortality. Late-gestation and early-lactation supplemental feeding of ewes results in increases in weaning weights of their lambs.

• Asian-Australasian Journal of Animal Sciences
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• 제9권5호
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• pp.591-595
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• 1996

This paper presents results from a study of the age and weight at puberty of Thai does. A randomized block $3{\times}3$ factorial design was used. Factors were genotype (Thai Native; TN, 75% TN $\times$ 25% Anglo-Nubian; AN and 50 TN $\times$ 50% AN does), year of kidding (1989, 1990 and 1991) and birth type (single or twin) as a block. It was shown that there were no significant (p > 0.05) difference in age at puberty among genotypes ($167.6{\pm}6.0$, $157.6{\pm}7.0$ and 160.0 = 4.7 days for TN, 75% TN $\times$ 25% AN and 50% TN $\times$ 50% AN does, respectively) and between birth types. However, does kidded in 1991 had significantly (p < 0.01) longer age at puberty ($194.0{\pm}7.4$ days) than did does kidded in 1989 ($143.0{\pm}4.5$ days) or in 1990 ($148.1{\pm}5.7$ days). There was an interaction effect between genotype and year. Fifty % TN $\times$ 50% AN does had significantly (p < 0.05) higher weights at pubery ($17.2{\pm}0.4kg$) than those of TN($14.3{\pm}0.5kg$ and 75% TN $\times$ 25% AN ($14.4{\pm}0.6kg$) does. There was no significant difference in weights at puberty between TN and 75% TN $\times$ 25% AN does. There was no effect of birth type on weight at puberty. There was an interaction effect between genotype and year of kidding on age and weight at puberty and on growth rate from birth to weaning.

• Journal of Chest Surgery
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• 제30권12호
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• pp.1167-1174
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• 1997

We retrospectively reviewed a series of 20 patients treated with delayed sternal closure during a 5-year period from 1991 to 1996. Of the 2675 patients with cardiovascular su gery 20 underwent this procedure. Male and female ratio was 11:9, mean age was 6.4 months(range 7 days to 5 years). The indications included unstable hemodynamic profiles after open heart surgery due to myocardial edema and poor lung compliance(15), necessity of mechanical ventricular assist device due to weaning failure(3), and hypoxia after PAB(2). Sternum was closed at a mean interval of 102(range 4 to 213) hours after operation. During delayed sternal closure, central venous pressure was elevated(p<0.05). Mediastinitis and other wound problems did not occur. Sepsis developed in 2 patients and microorganism was confirmed in one of the two patients. Five patients died(mortality 25%). And two of 15 discharged patients died during follow-up period. Cumulative survival rate was 65.0% at 12 months and also 65.0% at 24 months.(Standard error was 10.7%) Delayed sternal closure Is considered to be a good method to decompress the hemodynamically compromised heart. Without that, it is not f asible to come off bypass or to decompress the heart. Of course. careful selection of the indication is imperative.

• Tuberculosis and Respiratory Diseases
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• 제73권2호
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• pp.100-106
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• 2012

Background: There are a plethora of literatures showing that high-intensity intensive care unit (ICU) physician staffing is associated with reduced ICU mortality. However, it is not widely used in ICUs because of limited budgets and resources. We created a critical care team (CCT) to improve outcomes in an open general ICU and evaluated its effectiveness based on patients' outcomes. Methods: We conducted this prospective, observational study in an open, general ICU setting, during a period ranging from March of 2009 to February of 2010. The CCT consisted of five teaching staffs. It provided rapid medical services within three hours after calls or consultation. Results: We analyzed the data of 830 patients (157 patients of the CCT group and 673 patients of the non-CCT one). Patients of the CCT group presented more serious conditions than those of the non-CCT group (acute physiologic and chronic health evaluation II [APACHE II] 20.2 vs. 15.8, p<0.001; sequential organ failure assessment [SOFA] 5.5 vs. 4.6, p=0.003). The CCT group also had significantly more patients on mechanical ventilation than those in the non-CCT group (45.9% vs. 23.9%, p<0.001). Success rate of weaning was significantly higher in the CCT group than that of the non-CCT group (61.1% vs. 44.7%, p=0.021). On a multivariate logistic regression analysis, the increased ICU mortality was associated with the older age, non-CCT, higher APACHE II score, higher SOFA score and mechanical ventilation (p<0.05). Conclusion: Although the CCT did not provide full-time services in an open general ICU setting, it might be associated with a reduced ICU mortality. This is particularly the case with patients on mechanical ventilation.

• Journal of Microbiology and Biotechnology
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• 제25권4호
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• pp.431-438
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• 2015

Ever since the ban on antibiotic growth promoters (AGPs), the livestock death rate has increased owing to pathogenic bacterial infections. There is a need of developing AGP alternatives; however, the mechanisms by which AGP enhances livestock growth performance are not clearly understood. In this study, we fed 3-week-old swine for 9 weeks with and without AGPs containing chlortetracycline, sulfathiazole, and penicillin to investigate the effects of AGPs on swine gut microbiota. Microbial community analysis was done based on bacterial 16S rRNA genes using MiSeq. The use of AGP showed no growth promoting effect, but inhibited the growth of potential pathogens during the early growth stage. Our results showed the significant increase in species richness after the stabilization of gut microbiota during the post-weaning period (4-week-old). Moreover, the swine gut microbiota was divided into four clusters based on the distribution of operational taxonomic units, which was significantly correlated to the swine weight regardless of AGP treatments. Taxonomic abundance analysis indicated a negative correlation between host weight and the abundance of the family Prevotellaceae species, but showed positive correlation to the abundance of the family Spirochaetaceae, Clostridiaceae_1, and Peptostreptococcaeae species. Although no growth performance enhancement was observed, the use of AGP inhibited the potential pathogens in the early growth stage of swine. In addition, our results indicated the ecological succession of swine gut microbiota according to swine weight. Here, we present a characterization of swine gut microbiota with respect to the effects of AGPs on growth performance.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1379-1382
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• 2005

High prolificacy in Garole sheep is due to existence of FecB mutation in an autosomal gene, bone morphogenetic protein receptor. The mutation enhances ovulation rate and in turn litter size in Garole sheep. Garole sires were crossed with non-prolific Malpura ewes with the aim to introduce prolificacy into Garole${\times}$Malpura (G${\times}$M) crosses through FecB introgression programme. In the present study, the effect of carrying booroola allele on litter size and live body weight was analyzed. The average litter size at birth was found to be 1.87 and 1.48 in the Garole and the G${\times}$M crosses, respectively. At weaning, 6-month, 9-month and 12-month of age, body weights were not affected by the presence of booroola allele (p>0.05); however, a significant effect (p<0.05) was found on body weight at birth in G${\times}$M crosses. In Garole sheep, no significant effect of FecB was observed on live weights in any age group. The interaction between the genetic group and the FecB genotype was also found to be non-significant.