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Analytical studies of bovine mastitis management by standard plate counts(SPC) and somatic cell counts(SCC) (젖소 유방염 관리에 따른 세균 및 체세포수 등급 실태 조사 분석)

  • 허정호;정명호;박영호;조명희;이주홍
    • Korean Journal of Veterinary Service
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    • v.21 no.3
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    • pp.285-300
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    • 1998
  • 1. The number of average milking cows, clinical forms of mastitis, mastitis-developing cows, and cows killed by mastitis a year were 25.7, 1.8(7%), 6.3(26%), and 2.7(10.1%)heads, respectively. The annual grade changes of standard plate counts(SPC) and somatic cell counts(SCC) showed the grade 1A of SPC diminished sharply from April to August, we think it was due to the lack of proper management in farming season and the grade 3 of SCC indirectly influenced increased in huge during August. 2. The average number of parturitions of farms was 2.3, but 50% of below 1 parturition were 22 farms(31%), 50% of above 3 parturitions were 16(23%) out of 71 farms. According to grades of the number of parturitions of milking cows per each farm, the farms' grades recording 3 parturitions and 50% were little bit excellent. 3. The actual situation research of foremilking CMT revealed 35 out of 74 farmer didn't do CMT Among them(35 out of 74 farmers), 80% did not test thanks to the troublesome process of the CMT. SCC grade 3, among farms who did foremilking CMT once or twice a month and who did not were 29% and 40% respectively and SPC grade 1A were 55% and 9%, respectively. 4. The research of actual situation on milking management let us know 29 farms(39%) did not do lastmilking, 37 farms(49%) usually did overmilking, and 34 farms(46%) did milking for 4 or 5 minutes. Grades according to average requiring times of milking showed SCC grade 1 of farms milking within 7 minutes was 11% and SPC grade 1A was 34%, on the other side, farms milking more than 7 minutes were 0% in SCC grade 1 and 13% in SPC grade 1A. Grades according to the starting time of milking after rubbing teats showed SPC grade 1A of farms starting milking at about 1 minute and over 2 minutes were 50% and 20%, respectively. 5. The research of actual situation on hygienic milking management uncovered 65 farms(88%) were using one towel which was used in washing teats and udders to wash more than 3 to 4 cows, and 53 farms(72%) were using one dried towel to dry udders not for each cow but for more than 3 to 4 cows after washing. Also, on milking turns disclosed 30 farms(40%) were milking cows in the order of incoming without isolation of a dominant group. According to grades of towels used in washing teats and udders, farms using a towel for each cow were 56% and a towel for over 3 cows were 31% in SPC grade 1A. According to using-or-not grades of dried towels after washing udders, farms using a towel for each cow were 79% and a towel for over 3 cows were 21% in SPC grade 1A. 6. Farms doing teat-dipping before milking were 7(10%), not doing teat-dipping after milking, or doing sometimes were 9(12%), and doing right after milking were 57(77%). And farms doing teat-dipping after dry cows and before delivery were 21(28a ). Farms using bethadine as an antiseptic solution were 70(95%), 40 farms(59%) diluted it with water as weak as 5 to 10 times, and on drying cows 64 farms(87%) slowly did it more than 2 days. Grade 1A of SPC of farms doing teat-dipping at every milking was 38%, farms doing occasionally or not was 33%, and farms doing it right after milking was 37% and doing after milking more than 5 cows was 20%. Grade 1A of SPC among farms diluting bethadine 5 times and diluting 5 to 10 times with water were 36% and 33%, respectively, and Grade 3 of SCC were 35% and 32%, respectively. 7. Studies on nonlactating period medical treatment, as the cows were on dry, 54 farms treated with their own hands.73 farms(98%) had bovine mastitis treated for themselves. And on applying medicines against mastitis, 55 farmers chose them on the basis of their own experience, 42 farms(57%) were treated more than 3 days. 41 farms(55%) dumped away the mastitis infected milk separately, 24 farms(32%) were feeding and milking at the same time. 8. Fifty-six farms(76%) always washed and disinfected milking machines after milking. Farms using the milking machines at low, or variable vacuum pressures, or at the vacuum pressure, set at the moment of its installation were 31(42%), and farms that did not know pulsation ratio were 27(37%). Farms changing liners when they were torn 8(11%), 58 farms(78%) said they checked milking system when there were wrong with them, 31 farms(42%) changed milking hoses when they found out problems, and 42 farms(57%) cleaned vacuum and milking systems when they felt dirty. The SPC grade 1A of farms washing and sterilizing milking machines was 38% and farms only washing was 28%.

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The Effects of Sight Alignment Method on Pistol Shooting Records of Security Guard (경호요원 권총사격시 조준선정렬방식과 기록변화와의 관계)

  • Lee, Sang-Chul;Shin, Seung-Cheol
    • Korean Security Journal
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    • no.14
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    • pp.367-388
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    • 2007
  • Some of individuals working at various fields of security guard are given the legal right to use a gun to cope with expected risks and crimes. Not only in Korea but also throughout the world, a pistol is being used as a device for the workers of security guard such as the army, the police and intelligence agencies to keep the law and social order. A pistol, which is regarded as the last safety measure of security guard, is easy to carry but, because of its short barrel for high portability, its hit ratio and long-range shooting performance are much lower than ordinary guns. Despite such weak points, a pistol can be a great tool because of its high portability and if the users make steady efforts to improve their skills and, for this reason, it has been being used widely until now. National agency workers' pistol shooting skills depend on several important factors, but for the fast and accurate shooting, the mot important factor is how to aim at the target. Because it takes a relatively longer time to align the sight on the target, the change of the sight alignment method is expected to improve pistol shooting skills a lot. In Korea, however, most of national agency training centers teach the basic aiming method that crosses the foresight and the backsight and aligns the crossing to the target. That is, they teach the sight alignment method for fixed targets. Some agencies in Korea and foreign countries that have used guns more frequently and longer than us are teaching different sight alignment methods as well. Representative ones of them are aiming only with the foresight and pointing shooting. These aiming methods are expected to produce good results against moving targets. While the basic sight alignment method has to cross the foresight and the backsight and then to align the crossing to the target, so takes a longer time than other aiming methods, the other two aiming methods are expected to be effective in fields where both swiftness and accuracy are require. Because domestic agencies are teaching mainly the basic sight alignment method, the present study purposed to examine the effects of different aiming methods on shooting records and ultimately to contribute to national agency workers' operation. For this purpose, we selected 20 policemen from local police commandos under the National Police Agency who were accustomed to pistol shooting and measured their pistol shooting. According to the results, the pointed shooting method showed the best record in 5m short.range shooting. As to hit ratio in 15m shooting, which is relatively long.range for pistol shooting, the basic aiming method showed the best record, but the results of statistical analysis showed that pointed shooting is most superior. Of course, the results were obtained from a group who were used to and skillful in pistol shooting, but they are considered meaningful in that all the police and national agency workers should have such high shooting skills.

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Islamist Strategic Changes against U.S. International Security Initiative (미국(美國)의 대외안보전략(對外安保戰略)에 대응한 이슬람Terrorism의 전술적(戰術的) 진화(進化))

  • Choi, Kee-Nam
    • Korean Security Journal
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    • no.14
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    • pp.517-534
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    • 2007
  • Since the beginning of human society, there have always been struggles and competitions for survival and prosperity, terrorism is not a recent phenomenon, however in modern times it has progressed to reflect the advances in civilization and power structures. At the time of the 9.11 terrorist attacks in the U.S. A., a new world order was in the process of being established after the breakdown of the Cold War era. The attacks drove both the Western and the Islamic worlds into heightened fear of terrorism and war, which threatened the quality of life of the whole mankind. Through two war campaigns against the Islamic world, it seems the U.S. has been pushing its own militaristic security road map of the Greater Middle East democratic initiative, justifying it as a means to retaliate and eradicate the terrorist threats towards themselves. However, with its five-year lopsided victories that cost the nation almost four thousand military casualties, and the war expenses that could match the Vietnam war, the U.S. does not yet seem to be totally emancipated from the fears of terrorism. Terrorism, in itself, is a means of resisting forced rules a form of alternative competition by the weak against the strong, and a way of expressing a dismissive response against dictatorial ideas or orders which allow for no normal changes. Intrinsically, the nature of terrorism is a reaction opposing power logics. Confronted with the absolute military power of the U.S., the Islamic strategies of terrorism have begun to rapidly evolve into a new stage. The new strategies take advantage of their civilization and circumstances, they train and inspire their front-line fighters on the Internet, and issue their orders through the clandestine network of the Al Qaeda operatives. These spontaneously generated strategies have been gained speed among the second, and third Islamic generations, many of whom are now spread throughout western societies. This represents a failure of the power-driven, one-sided overseas security initiatives by the U.S., and is creating a culture of fear and distrust in western societies. It is feared that the U.S. war campaigns have made the clash of religions far worse than before, and may ever lead to global ethnic separations and large-scale population movements. Eventually, it may result in the terrorist groups, enlarged and secretly supported by the huge sums of oil money, driving all mankind into a series of irreparable catastrophes.

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A STUDY ON THE BONDING OF COMPOMER TO DECIDUOUS DENTIN (컴포머와 유치 상아질의 결합에 관한 연구)

  • Kim, Jee-Tae;Kim, Yong-Kee;Kim, Jong-Soo;Kwon, Soon-Won
    • Journal of the korean academy of Pediatric Dentistry
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    • v.29 no.4
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    • pp.509-518
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    • 2002
  • The purpose of this study was to evaluate the bonding of compomer to deciduous dentin which is known to have been developed to improve the weak properties of glass ionomer cement and composite resin. 120 sound primary molars were used for the shear bond strength test and another 24 for the scanning electron microscopic evaluation. Each material was ailed into polyethylene mold attached to exposed dentinal surface($3{\times}4mm$ in diameter) of sample blocks. Shearbond strength was measured using Universal testing machine and data were analyzed statistically with Oneway-ANOVA and Scheffe test. Scanning electron microscopic observation was performed in order to evaluate the pattern of distribution and penetration of resin tags and hybrid layer. Compomer groups(II-V) showed significantly higher bond strength values than glass ionomer group(I)(p<.05). Etching-compomer groups(III, V) showed the significantly higher bond strength than non-etching compomer groups(II, IV)(p<.05), but slightly lower values than composite resin group(VI) with no statistically significant difference(p>.05). No significantly different bond strength was found between compomer groups of different bonding system(p>.05). Scanning electron micrographs showed more irregular distribution of short and thin resin tags in non-etching compomer groups(II, IV) whereas the more regular and intimate distribution of long and thick tags in etching compomer groups(III, V) and composite resin group(VI). The evaluation of hybrid layer also showed more regular formation of thicker layer in etching compomer groups(III, V). Based on the results of present study, the use of compomer as an esthetic restorative material for primary molars might be justified.

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A Study on the Current State and Effect of Entrepreneurship Education in Major Countries: Comparison of the 2016 Global Entrepreneurship Index (주요 국가의 기업가정신 교육 현황 및 효과 연구: 2016년 글로벌 기업가정신 지수의 비교)

  • Nam, Jungmin;Lee, Hwansoo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.12 no.6
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    • pp.111-122
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    • 2017
  • This study analyzes the current state of entrepreneurship education and start-up foundations by country in order to find ways to improve the domestic entrepreneurial environment and to promote the recognition of desirable entrepreneurship practices. It also investigates the relationship between entrepreneurship, entrepreneurial will, and the level of opportunity-based entrepreneurships, by using data from the 2016 Global Entrepreneurship Trend Report (GETR). First, the results show the urgent need for the expansion of entrepreneurship education in Korea. In the GETR category of 'experience of entrepreneurship education in elementary, middle and high schools', Korea was ranked very low (19th place), among the 20 countries. In the 'college' and 'lifelong entrepreneurship education' categories, it procured a mid-level ranking (15th). While entrepreneurship education for all ages is being promoted globally, entrepreneurship education for middle-aged individuals in Korea is relatively weak. This implies that the expansion of entrepreneurship education to lifelong education and education for employees and retirees is required. Second, the individual's entrepreneurial intention in Korea was 3.8 points, implying a mid-level ranking (15th), and it ranked the lowest in terms of opportunity-based entrepreneurship (20th). In comparison to China (4.55) and the United States (4.01), the entrepreneurial intention of Koreans was found to be low. The level of opportunity-driven entrepreneurship was also found to be very low, compared to China (4.35), Japan (4.04) and the United States (4.59). In general, the proportion of the level in opportunity-driven entrepreneurship, increases from the factor-driven and efficiency-driven, to the innovation-driven type. In Korea, the percentage of entrepreneurial ventures centered around involuntary entrepreneurship and small businesses is high. It is also interpreted that opportunity-based entrepreneurships are low in number because of this high proportion of involuntary start-up and small businesses. Last, the entrepreneurial intention in all types (factor-driven, efficiency-driven, and innovation-driven) was exceptionally high. It has been confirmed that exposure to all entrepreneurship education (elementary, junior high, university, and lifelong education) in innovation-driven countries, greatly increases entrepreneurial intention. In the case of Korea, which is an innovation-driven country, qualitative improvement based on quantitative expansion of entrepreneurship education is expected to be a major driving force for individuals' entrepreneurial intention to obtain a mid-level ranking (15th).

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An Analysis for Effects of Stain Family Drugs on Osteogenic Differentiation using Human Periosteum-derived Mesenchymal Stem Cells (스타틴(statin) 약물이 성체줄기세포의 골분화에 미치는 영향)

  • Moon, Dong Kyu;Yun, Jeong-Won;Kim, Bo Gyu;Lee, A Ram;Moon, Sun Young;Byun, June-Ho;Hwang, Sun-Chul;Woo, Dong Kyun
    • Journal of Life Science
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    • v.29 no.12
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    • pp.1337-1344
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    • 2019
  • Osteoporosis is characterized by a reduction in bone mass and typically manifests as an increase in fractures. Because this disease is common in elderly populations and lifespans are rapidly increasing, the incidence of osteoporosis has also grown. Most drugs currently used for osteoporosis treatment target osteoclasts in the bone tissue to prevent absorption. However, these medications also cause certain side effects and, furthermore, cannot increase bone mass. Thus, in order to control osteoporosis, regenerative medicine that utilizes adult stem cells and osteoblasts has been extensively studied. Statins, also known as 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors, are cholesterol-lowering drugs that have been widely prescribed for cardiovascular diseases. Interestingly, recent studies have reported the beneficial effects of various statins on bone formation via the activation of osteoblasts. Thus, the current study investigated the effects of seven statin-family drugs on osteoblast activity during osteogenic differentiation using adult stem cells from human periosteal tissue. Specifically, statin effects on alkaline phosphatase activity, an early marker of bone cell differentiation, and on calcium deposit, a late marker of bone cell differentiation, were assessed. The results demonstrate that some statins (for example, pitavastatin and pravastatin) have a weak but positive effect on bone formation, and the findings therefore suggest that statin treatments can be a novel modulator for osteogenic differentiation and regenerative medicine using periosteal stem cells.

$Ca^{2+}-induced$ Inhibition of Microsomal ATPases in Soybean Roots (콩 뿌리조직에서의 $Ca^{2+}$에 의한 마이크로솜 이온펌프 활성저해)

  • Cho, Kwang-Hyun;Cho, Kyoung-Soo;Lee, Eun-Hyoung;Kim, Young-Kee
    • Applied Biological Chemistry
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    • v.40 no.3
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    • pp.202-208
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    • 1997
  • In order to investigate the mechanisms of epithelial ion transports, microsomes of soybean roots were prepared and the activity of microsomal ATPases was measured by an enzyme-coupled assay. The effects of various ions were evaluated on the total activity of microsomal ATPases and the average activity was 190 nmol/min/mg protein in the control solution containing $10\;mM\;Na^+\;and\;120\;mM\;K^+$. The activities were increased to 150% and decreased to 63% of the control activity in the solution containing $130\;mM\;K^+$ without $Na^+$ and in the solution containing $130\;mM{\;}Na^+$ without $K^+$, respectively. In general, the activity of microsomal ATPase was increased by$K^+$ in a concentration-dependent manner The activity was also increased at lower pH and relatively higher activities were observed in the pH range of $6{\sim}7$. However, the activity was decreased at weak alkaline $pH\;and{\sim}80%$ of the activity was inhibited at pH 9. Since intracellular $Ca^{2+}$ has been known to control the activity of various enzymes, we have investigated the effects of intra-and extrarnicrosomal $Ca^{2+}$ on the activity of microsomal ATPases. The maximal activity was obtained at the extrarnicrosomal $Ca^{2+}$ concentrations below 1 nM. The activity was gradually decreased by increasing $‘Ca^{2+}’$ concentration and 50% inhibition was observed at ${\sim}500{\;}{\mu}M{\;}Ca^{2+}$. The increase in luminal $Ca^{2+}$ concentration also inhibited the activity of microsomal ATPase. When the influx of external $Ca^{2+}$ was induced by $Ca^{2+}$ ionophore A23187 treatment, the activity was decreased by 30%; however, it was recovered by EGTA-induced chelation of $Ca^{2+}$. These results suggest that the presence of $Ca^{2+}$ regulation sites on both cytoplasmi and luminal sides of microsomal ATPases.

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Sedimentological Characteristics of Surface Sediments in the Southwestern Sea off Cheju Island, Korea (제주도 서남해역의 해저퇴적물 특성)

  • Youn, Jeung-Su;Kim, Soung-Bok;Koh, Gi-Won
    • 한국해양학회지
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    • v.24 no.3
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    • pp.132-147
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    • 1989
  • A total of 83 surface sediments and 55 sea water samples, collected from the southwestern sea of Cheju Island, were analyzed in order to understand their textural characteristics, geochemical composition and the clay mineralogical features. The sediments were subdivided into ten textural classes, namely clayey sand, slightly gravelly muddy sand, sandy clay, clay and mud. The coarse and fine-grained mixed sediments are distributed in the northern part and around the Island, whereas the fine-grained deposits are mainly distributed in the central and southern parts of the study area; small scale mud patches are distributed in the southwestern and northern parts of Cheju Island. The high concentration of total suspended matter in study area gradually increase toward the southwestern and northwestern offshore area. The concentration of geochemical elements is as follow: the content of Mn, Al, Zn, Cr, Cu and Sn increase toward the southern part which is covered mainly with fine-grained deoposits, whereas the content of Ca, Mg and Ag is higher in the northern area; the elements such as Ni, Na, Fe and Pb are more concentrated relatively in muddy deposits rather than in sandy sediments. The light minerals such as Na-Ca feldspars show a high content around the Socotra Rock, toward the Soheugsan and Cheju Islands, but the K-feldspars are relatively high around the Cheju Island. It was noticed that the provenance of these sediments is partly influenced by the geological characteristics near the island. X-ray diffractogram for clay minerals from the southeastern mud patch and around the Soheugsan Island shows the diagnostic calcite peak indicating that the greater part of these clay fraction may have been derived from present and ancient Hwangho River. The high concentration of smectite in the northern part near the Cheju and around the Soheugsan Islands, eastern side of Socotra Rock probably result from supplies smectite altered from volcanic materials distributed in the Cheju Island and Socotra Rock, whereas the samples near the Chuja and northern parts of the Cheju Island contain weak calcite peak and high concentration of kaolinite and chlorite which is closely related to the geolgical characteristics on the adjacenting land area.

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Seasonal sea Level oscillations in the East Sea (Sea of Japan) (동해 해수면의 계절적인 변동에 대하여)

  • OH, IM SANG;RABINOVICH, ALEXANDER B.;PARK, MYOUNG SOOK;MANSUROV, ROALD N.
    • 한국해양학회지
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    • v.28 no.1
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    • pp.1-16
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    • 1993
  • The monthly mean sea levels at 48 stations located at the East and Yellow Seas coasts of Korea, Russia and Japan are processed to investigate seasonal sea level variations. The strong seasonal variations are found to be at the west coast of Korea (42.1 cm in Kunsan), in the region of the Korea strait and near the southern part of Primorye (30-33 cm); the weak ones near the southwestern coast of the Sakhalin Island (10-12 cm). Practically for the whole study area except the southwest Sakhalin, the general picture of the seasonal sea level changes is alike: the mean sea level rises in summer-autumn and falls in winter-spring. The spectral analysis of the records also shows that the seasonal oscillations strongly dominate in the sea level variations, more than 80% or total energy in the southern part of the investigated region and 50-70% in the northern part relate to these oscillations. The annal peak significantly prevails in spectra of the monthly sea levels for the majority of stations, the semiannual peak is also well manifested, but the seasonal peaks of higher order (corresponding to the periods of four and three months) reveal only at some records. The maximal amplitudes of annual component by a least square method are found at the Yellow Sea coast of Korea (20-21 cm) and also near the Japanese coast of the korea Strait (19-19 cm). The semiannual component has the maximal amplitudes (3-4 cm) near the south and southwestern coasts of the Sakhalin Island. The annual range of the sea levels is much weaker here than in the other regions, the relative investment of the seasonal oscillations in total energetic budget is only 35-40%, annual ($A_1$) and semiannual ($A_2$) components have nearly the same amplitude (seasonal factor $F=A_1/A_2=0.9-1.2$). On the basis of the present examination on sea level changes together with the results of Tomizawa et. al.(1984) the whole investigated area may be divided into 10 subregions, 2 of them are related to the Yellow Sea and Western part of the Korea Strait (Y1, Y2), the other ones (E1-E8) to the East Sea.

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A Study of the Utilization of Feldspathic Sand as a Fortified Functional Filtering Material for Water Purification (고 기능성 수질 정화 여과재로서의 장석질 모래 활용연구)

  • 고상모;송민섭;홍석정
    • Journal of the Mineralogical Society of Korea
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    • v.16 no.4
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    • pp.283-293
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    • 2003
  • Domestic water treatment plants operate the rapid and slow filtering system using the filtering sands. Most of them are composed of beach sands, which have less sorption capacity of heavy metals as well as organic contaminants. Therefore, the development of fortified functional filtering materials with high removal capacity of organic and inorganic contaminants is needed to prevent the unexpected load of contaminated source water. This study aims to test the hydrochemical change and the removing capacity of heavy metals such as Cd, Cu, and Pb on the Jumunjin sand, feldspathic sand(weathering product of Jecheon granite), feldspathic mixing sand I(feldspathic sand mixed with 10 wt% zeolite), and feldspathic mixing sand II (feldspathic sand mixed with 20 wt% zeolite). Feldspathic mixing sand I and II showed the eruption of higher amounts of cations and anions compared with the Jumunjin sand and feldspathic sand. They also showed higher eruption of Si, Ca, $SO_4$ ions than that of Al, $NO_3$, Fe, K, Mg, and P. Feldspathic mixing sand II caused higher eruption of some cations of Na, Ca, Al than feldspathic mixing sud I, which is the result controlled by the dissolution of zeolite. Jumunjin sand and feldspathic sand showed very weak sorption of Cd, Cu and Pb. In contrast to this, feldspathic mixing sand I and II showed the high sorption and removal capacity of the increasing order of Cd, Cu and Pb. Feldspathic mixing sand II including 20% zeolite showed a fortified removal capacity of some heavy metals. Therefore, feldspathic mixing sand mixed with some contents of zeolite could be used as the fortified filtering materials for the water filtering and purification in the domestic water treatment plants.