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Survey of Beet Red Contents in Foods using TLC, HPLC (TLC, HPLC를 이용한 식품 중 비트레드 함량조사)

  • Jang, Yaung-Mi;Lee, Tal-Soo;Hong, Ki-Hyoung;Park, Sung-Kwan;Park, Sung-Kug;Kwon, Yong-Kwan;Park, Jae-Seok;Chang, Sun-Young;Hwang, Hye-Shin;Kim, Eun-Jeong;Han, Yeun-Jeong;Kim, Byung-Sub;Won, Hye-Jin
    • Journal of Food Hygiene and Safety
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    • v.20 no.4
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    • pp.244-252
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    • 2005
  • This study was performed far development of new analytical method of beet red in foods. In this study, analysis of beet red in foods has been carried out by detection of betanine and isobetanine, the main color component of beet red as indicator compounds. The qualitative analysis technique consisted of clean-up of the colors with a $C_{18}$ cartridge, separation of the colors by cellulose TLC plate using acetone:3-methyl-1-butanol:distilled water (7:7:6) as a solvent system. Also, the quantitative analysis was performed using X-terra RP at wavelength 538 nm and $0.1\%$ phosphoric acid : methanol (90:10) as a solvent. The quantitative results of beet .ed were as follows:900.22$\∼$27701.60 $\mu$g/g for 60 item in nutrient supplement food, $21.95\∼713.40{\mu}g/g$ for 30 items and N.D. for 18 items in cindy, and $155.85{\∼}505.37{\mu}g/g$ for 12 items in ice creams, $43.52\∼64.75{\mu}g/g$ for 18 items and N.D. for 54 item in sauce, N.D. for 12 items in retort food.

Recent Variations of UV Irradiance at Seoul 2004~2010 (서울의 최근 자외선 복사의 변화 2004~2010)

  • Kim, Jhoon;Park, Sang Seo;Cho, Nayeong;Kim, Woogyung;Cho, Hi Ku
    • Atmosphere
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    • v.21 no.4
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    • pp.429-438
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    • 2011
  • The climatology of surface UV radiation for Seoul, presented in Cho et al. (1998; 2001), has been updated using measurement of surface erythemal ultraviolet (EUV) and total ultraviolet (TUV) irradiance (wavelength 286.5~363.0 nm) by a Brewer Spectrophotometer (MK-IV) for the period 2004~2010. The analysis was also carried out together with the broadband total (global) solar irradiance (TR ; 305~2800 nm) and cloud amount to compare with the UV variations, measured by Seoul meteorological station of Korean Meteorological Agency located near the present study site. Under all-sky conditions, the day-to-day variability of EUV exhibits annual mean of 98% in increase and 31% in decrease. It has been also shown that the EUV variability is 17 times as high as the total ozone in positive change, whereas this is 6 times higher in negative change. Thus, the day to day variability is dominantly caused rather by the daily synoptic situations than by the ozone variability. Annual mean value of daily EUV and TUV shows $1.62kJm^{-2}$ and $0.63MJm^{-2}$ respectively, whereas mean value of TR is $12.4MJm^{-2}$ ($143.1Wm^{-2}$). The yearly maximum in noon-time UV Index (UVI) varies between 9 and 11 depending on time of year. The highest UVI shows 11 on 20 July, 2008 during the period 2004~2010, but for the period 1994~2000, the index of 12 was recorded on 13 July, 1994 (Cho et al., 2001). A 40% of daily maximum UVI belongs to "low (UVI < 2)", whereas the UVI less than 5% of the maximum show "very high (8 < UVI < 10)". On average, the maximum UVI exceeded 8 on 9 days per year. The values of Tropospheric Emission Monitoring Internet Service (TEMIS) EUV and UVI under cloud-free conditions are 1.8 times and 1.5 times, respectively, higher than the all-sky measurements by the Brewer. The trend analysis in fractional deviation of monthly UV from the reference value shows a decrease of -0.83% and -0.90% $decade^{-1}$ in the EUV and TUV, respectively, whereas the TR trend is near zero (+0.11% $decade^{-1}$). The trend is statistically significant except for TR trend (p = 0.279). It is possible that the recent UV decrease is mainly associated with increase in total ozone, but the trend in TR can be attributed to the other parameters such as clouds except the ozone. Certainly, the cloud effects suggest that the reason for the differences between UV and TR trends can be explained. In order to estimate cloud effects, the EUV, TUV and TR irradiances have been also evaluated for clear skies (cloud cover < 25%) and cloudy skies (cloud cover ${\geq}$ 75%). Annual mean values show that EUV, TUV and TR are $2.15kJm^{-2}$, $0.83MJm^{-2}$, and $17.9MJm^{-2}$ for clear skies, and $1.24kJm^{-2}$, $0.46MJm^{-2}$, and $7.2MJm^{-2}$ for cloudy skies, respectively. As results, the transmission of radiation through clouds under cloudy-sky conditions is observed to be 58%, 55% and 40% for EUV, TUV and TR, respectively. Consequently, it is clear that the cloud effects on EUV and TUV are 18% and 15%, respectively lower than the effects on TR under cloudy-sky conditions. Clouds under all-sky conditions (average of cloud cover is 5 tenths) reduced the EUV and TUV to about 25% of the clear-sky (cloud cover < 25%) values, whereas for TR, this was 31%. As a result, it is noted that the UV radiation is attenuated less than TR by clouds under all weather conditions.

Luminescence and Photostimulated Luminescence of $Eu^{2+}$ in Alkaline Earth Chlorides (염화알칼리토금속 화합물에 도핑된 $Eu^{2+}$ 이온의 광발광 및 광자극발광특성)

  • Kim, Sung-Hwan;Kim, Sun-Chil
    • Journal of radiological science and technology
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    • v.29 no.3
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    • pp.141-145
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    • 2006
  • In this study, $Eu^{2+}$ ion was used as an activator in order to enhance the PL(photoluminescene) and PSL(photostimulated luminescence) intensity of $MCl_2:Eu^{2+}$(M = Ca, Sr, Ba)phosphors and the chracteristics of PL and PSL of the phosphors were investigated. The emission of $MCl_2:Eu^{2+}$(M = Ca, Sr, Ba) phosphors shows a shift wavelength when the host caution changes. The optimal preparing conditions of $CaCl_2:Eu^{2+}$ phosphor were 0.5 mol% of $EuCl_2$ and the sintering temperature were $745^{\circ}C$, 45 min. in $H_2$ atmosphere. The PL and PSL spectra of $CaCl_2:Eu^{2+}$ locate in the range of $365{\sim}388\;nm$, peaking at 370 and 380 nm. The optimal preparing conditions of $BaCl_2:Eu^{2+}$ phosphor were 0.5 mol% of $EuCl_2$ and the sintering temperature were $905^{\circ}C$, 45 min. in $H_2$ atmosphere. The PL and PSL spectra of $BaCl_2:Eu^{2+}$ locate in the range of $370{\sim}460\;nm$, peaking at 398 nm. The optimal preparing conditions of $SrCl_2:Eu^{2+}$ phosphor were 0.5mol% of $EuCl_2$ and the sintering temperature were $840^{\circ}C$, 45min. in $H_2$ atmosphere. The PL and PSL spectra of $SrCl_2:Eu^{2+}$ locate in the range of $380{\sim}440\;nm$, peaking at 407 nm. The dose response of the $MCl_2:Eu^{2+}$(0.5 mol%)(M = Ba, Sr) phosphors were linear within $0.25{\sim}200\;mGy$ of 100 kV X-ray and the PSL intensity of the $SrCl_2:Eu^{2+}$ and $BaCl_2:Eu^{2+}$ phosphors faded to approximately 60 and 40% respectively after 120 min at room temperature.

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Investigation of O4 Air Mass Factor Sensitivity to Aerosol Peak Height Using UV-VIS Hyperspectral Synthetic Radiance in Various Measurement Conditions (UV-VIS 초분광 위성센서 모의복사휘도를 활용한 다양한 관측환경에서의 에어로솔 유효고도에 대한 O4 대기질량인자 민감도 조사)

  • Choi, Wonei;Lee, Hanlim;Choi, Chuluong;Lee, Yangwon;Noh, Youngmin
    • Korean Journal of Remote Sensing
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    • v.36 no.2_1
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    • pp.155-165
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    • 2020
  • In this present study, the sensitivity of O4 Air Mass Factor (AMF) to Aerosol Peak Height (APH) has been investigated using radiative transfer model according to various parameters(wavelength (340 nm and 477 nm), aerosol type (smoke, dust, sulfate), aerosol optical depth (AOD), surface reflectance, solar zenith angle, and viewing zenith angle). In general, it was found that O4 AMF at 477 nm is more sensitive to APH than that at 340 nm and is stably retrieved with low spectral fitting error in Differential Optical Absorption Spectroscopy (DOAS) analysis. In high AOD condition, sensitivity of O4 AMF on APH tends to increase. O4 AMF at 340 nm decreased with increasing solar zenith angle. This dependency isthought to be induced by the decrease in length of the light path where O4 absorption occurs due to the shielding effect caused by Rayleigh and Mie scattering at high solar zenith angles above 40°. At 477 nm, as the solar zenith angle increased, multiple scattering caused by Rayleigh and Mie scattering partly leads to the increase of O4 AMF in nonlinear function. Based on synthetic radiance, APHs have been retrieved using O4 AMF. Additionally, the effect of AOD uncertainty on APH retrieval error has been investigated. Among three aerosol types, APH retrieval for sulfate type is found to have the largest APH retrieval error due to uncertainty of AOD. In the case of dust aerosol, it was found that the influence of AOD uncertainty is negligible. It indicates that aerosol types affect APH retrieval error since absorption scattering characteristics of each aerosol type are various.

Growth and Development of Cherry Tomato Seedlings Grown under Various Combined Ratios of Red to Blue LED Lights and Fruit Yield and Quality after Transplanting (다양한 조합의 적색과 청색 혼합 LED광에서 자란 방울 토마토 묘의 생육과 정식 후 수확량 및 품질)

  • Son, Ki-Ho;Kim, Eun-Young;Oh, Myung-Min
    • Journal of Bio-Environment Control
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    • v.27 no.1
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    • pp.54-63
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    • 2018
  • Red and blue lights are effective wavelengths for photosynthesis in plants. In this study, we determined the effects of various combined ratios of red to blue LEDs on the quality of cherry tomato seedlings prior to transplantation, and their subsequent effects on the yield and quality of tomato fruits after transplanting. Two-week-old cherry tomato seedlings (Solanum lycopersicum cv. 'Cuty') were cultivated under various combined ratios of red (R; peak wavelength 655 nm) to blue (B; 456 nm) LEDs [red:blue = 41:59 (59B), 53:47 (47B), 65:35 (35B), 74:26 (26B), 87:13 (13B), or 100:0 (0B)] and fluorescent lamps and raised for 27 days. The cherry tomato seedlings were subsequently transplanted into a venlo-type greenhouse and cultivated for 75 days. At the seedling stage, the shoot fresh weight of seedlings in all RB combined treatments, except 0B and 59B, was higher than that of the control after 27 days of LED treatment. Shoot dry weight and leaf area also showed trends similar to that of shoot fresh weight. The stem length was significantly higher in 13B, 26B, and 35B treatments compared with the control and other treatments. In particular, the stem length of 26B plants was approximately 3.2 times longer than that of 59B plants. At 37 days after transplanting, the number of nodes was significantly higher in 26B and 47B plants, and the plant height of 26B plants was significantly higher than that of control and 59B plants. Total fruit yield in 26B plants, which was the highest, was approximately 1.6 and 1.8 times higher than that in control and 59B plants, respectively. Thus, the results of this study indicate that various combined ratios of red to blue LEDs directly affected to the growth of cherry tomato seedlings and may also affect parameters of reproductive growth such as fruit yield after transplantation.

Simultaneous Determination of Carbohydrates and Organic Acids in Various Cultured Dairy Foods by High-Performance Liquid Chromatography: A Preliminary Study (다양한 낙농 발효유제품에서 HPLC를 이용하여 탄수화물과 유기산의 동시 검출에 관한 연구)

  • Kim, Dong-Hyeon;Hwang, Dae-Geun;Chon, Jung-Whan;Kim, Hyunsook;Kim, Hong-Seok;Song, Kwang-Young;Yim, Jin-Hyuk;Kim, Young-Ji;Kang, Il-Byung;Lee, Soo-Kyung;Seo, Kun-Ho
    • Journal of Dairy Science and Biotechnology
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    • v.33 no.4
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    • pp.263-269
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    • 2015
  • Various carbohydrates (lactose, glucose, and fructose), lactic acid, uric acid, and acetoin were separated on a ZORBAX Carbohydrate Analysis column using the Agilent 1200 HPLC ChemStation$^{TM}$, and were identified according to retention times with 325 Dual Wavelength UV-Vis Detector and Refractive Index Detector with 0.013 N $H_2SO_4$ at a flow rate of 0.8 mL/min. In addition, the lactase activity of four commercial probiotic lactic acid bacteria during 6-hour incubation was determined using a high-performance liquid chromatography (HPLC) method. Among the tested samples, Bifidobacterium animalis subsp. lactis showed the greatest lactase activity, followed by Lactobacillus rhamnosus and Lactobacillus casei, with Streptococcus salivarius subsp. thermophilus showing the lowest activity. Therefore, this HPLC technique shows potential for evaluating the fermentation processes of probiotic lactic acid bacteria and could simultaneously confirm the degree of ripening in various fermented dairy foods within only a half hour.

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정지궤도 통신해양기상위성의 기상분야 요구사항에 관하여

  • Ahn, Myung-Hwan;Kim, Kum-Lan
    • Atmosphere
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    • v.12 no.4
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    • pp.20-42
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    • 2002
  • Based on the "Mid to Long Term Plan for Space Development", a project to launch COMeS (Communication, Oceanography, and Meteorological Satellite) into the geostationary orbit is undergoing. Accordingly, KMA (Korea Meteorological Administration) has defined the meteorological missions and prepared the user requirements to fulfill the missions. To make a realistic user requirements, we prepared a first draft based on the ideal meteorological products derivable from a geostationary platform and sent the RFI (request for information) to the sensor manufacturers. Based on the responses to the RFI and other considerations, we revised the user requirement to be a realistic plan for the 2008 launch of the satellite. This manuscript introduces the revised user requirements briefly. The major mission defined in the revised user requirement is the augmentation of the detection and prediction ability of the severe weather phenomena, especially around the Korean Peninsula. The required payload is an enhanced Imager, which includes the major observation channels of the current geostationary sounder. To derive the required meteorological products from the Imager, at least 12 channels are required with the optimum of 16 channels. The minimum 12 channels are 6 wavelength bands used for current geostationary satellite, and additional channels in two visible bands, a near infrared band, two water vapor bands and one ozone absorption band. From these enhanced channel observation, we are going to derive and utilize the information of water vapor, stability index, wind field, and analysis of special weather phenomena such as the yellow sand event in addition to the standard derived products from the current geostationary Imager data. For a better temporal coverage, the Imager is required to acquire the full disk data within 15 minutes and to have the rapid scan mode for the limited area coverage. The required thresholds of spatial resolutions are 1 km and 2 km for visible and infrared channels, respectively, while the target resolutions are 0.5 km and 1 km.

Polymerization of HEMA by Electron beam Irradiation and Fabrication of Soft contact lens (전자빔조사에 의한 HEMA의 중합과 소프트콘택트렌즈 제조)

  • Hwang, Kwang-Ha;Shin, Joong-Hyeok;Sung, Yu-Jin;Jeong, Keun-Seung;Jun, Jin
    • Journal of Korean Ophthalmic Optics Society
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    • v.17 no.2
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    • pp.135-141
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    • 2012
  • Purpose: Polymerization of HEMA(2-hydroxyethyl methacrylate) which can be used in the soft contact lens has been performed by using electron beam(EB) irradiation, and examined the best condition for the polymerization. Comparing the physical properties of the contact lenses to the one fabricated by thermal polymerization method, we check the use possibility of the EB irradiation to the fabrication of the soft contact lens. Methods: We investigated the degree of polymerization of the HEMA according to the composition of the monomer, the additive ratio and the dose of electron beam (0~120 kGy). The degree of polymerization was measured depending on the EB dose to research the best synthetic condition under the EB irradiation. The physical properties of the contact lens such as water content(%), oxygen transmissibility(Dk/t) and optical transmittance were analysed by using the FT-IR results with comparing the two different polymerization method (thermal and electron beam polymerization) with same additive ratio. Results: When the dose of electron beam was above 100 kGy, the degree of polymerization of HEMA was above 99% with regardless using cross-linker and initiator. The water content of the lens fabricated by EB method showed 10% higher than the one by the thermal method which was 40%. The lens fabricated by EB method also showed higher oxygen transmissibility(Dk/t) as same with the water content, and showed twice higher value in the lens fabricated by pure HEMA. According to the FT-IR results, hydrophilic property of the lens fabricated by EB method was increased due to increasing the intermolecular hydrogen bonding. It showed above 90% optical transmittance in the visible range of wavelength on the contact lenses fabricated by the both of two different polymerization method. Conclusions: The polymerization of HEMA without cross-linker and initiator was successful above 100 kGy of EB irradiation. Moreover the lens fabricated from the polymer synthesized by pure HEMA with 100 kGy of EB showed the highest water content and oxygen transmissibility. Therefore EB irradiation is another possible method to synthesize the polymer which can be used for the soft contact lens.

Optical Properties of Sea Water in Sagami Bay, Japan (일본 상모만에서의 해수의 광학적 성질)

  • YANG Yong-Rhim
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.2
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    • pp.119-125
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    • 1987
  • Optical properties of sea water were studied in the Sagami Bay, Japan, based on the data obtained from six oceanographic stations in June, 1985. The observation of surface irradiance and underwater irradiance of sea water for eight kind of wavelengths (378, 422, 481, 513, 570, 621, 653, 677 nm) of sun light was conducted using the underwater irradiameter $(Isigawa\;\#\;SR-8)$. The mean attenuation coefficient of the sea water was appeared to be 0.166 $(0.061\~0.644)$ and the attenuation coefficient of the sea water for wavelength appeared such as 0.121 for 378 nm, 0.105 for 422 nm, 0.097 for 481 nm, 0.099 for 513 nm, 0.138 for 570 nm, 0.253 for 621 nm, 0.258 for 653 nm, 0.253 for 677 nm. The transparency was 12.9 m $(7.2\~18m)$, water color was $(5\~10m)$ in the study area and the sun altitude was $70.79^{\circ}\;(57.44^{\circ}\~78.42^{\circ}C)$ The relationship between attenuation coefficient (K) ana transparency (D) was $K=2.87/D(1.06/D\~5.48/D)$. The rates of light penetration for eight kind of wavelenths (378, 422, 481, 513, 570, 621, 653, 677 nm) were computed with reference to the surface light intensity respectively, The mean rate of light penetration in proportion to depths were $77.93\%\;(52.52\~94.06\%)$ in 1 m layer, $35.46\%\;(4.00\~73.64\%)$ in 5m layer, $18.71\%\;(0.24\~54.23\%)$ in 10m layer and $7.00\%\;(0.007\~27.58\%)$in 20m layer. The rate of light penetration at the transparency layer with reference to the surface light intensity was shown as $13.02\%\;(0.42\~34.78\%)$.

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Spatial Distribution of Pigment Concentration Around the East Korean Warm Current Region Derived from Satellite Data - Satellite Observation in May 1980 - (위성원격탐사에 의한 동한난류 주변 해역의 색소농도 공간적 분포 -1980년 5월 관측을 중심으로 -)

  • Kim Sang Woo;Saitoh Sei-ich;Kim Dong Sun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.3
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    • pp.265-272
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    • 2002
  • Spatial distribution of Phytoplankton Pigment Concentration (PPC) and Sea Surface Temperature (SST) around the East Korean Warm Current (EKWC) was described, using both Coastal Zone Color Scanner (CZCS) images and Advanced Very High Resolution Radiometer (AVHRR) images in May, 1980. Water mass in this region can be classified into five categories in the horizontal profile of PPC and SST, nLw (normalized water-leaving radiance) images: (1) coastal cold water region associated with concentrations of dissolved organic material or yellow colored substances and suspended sediments, (2) cold water region of thermal frontal occurred by a combination of phytoplankton absorption and suspended materials, (3) warm water overlay region by the phytoplankton absorption than the suspended materials; (4) warm water region occurred by the low phytoplankton absorption, and (5) offshore region occurred by the high phytoplankton absorption. In particular, the highest PPC (>2.0 mg/m^3) area appeared in the CZCS and AVHRR images with a band shaped distribution of the thermal front and ocean color front region, which is located the coastal cold waters alonB western thermal front of the warm streamer of the EKWC. In this region, the highest PPC occurred by a combination of the high absorption of the phytoplankton (443 nm) and highest reflectance of suspended materials (550 nm). Another high PPC ($\simeq$$6\;mg/m^3$) appeared in the warm water overlay region inside warm streamer. High phytoplankton pigment concentration of this region was corresponding to the short wavelength of 443 nm, which represented phytoplankton absorption of the CZCS image.