• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.108-109
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• 2013

Mitochondria play key roles in the production of cell's energy. Their dominant function is the synthesis of adenosine 5'-triphosphate (ATP) from adenosine diphosphate (ADP) and phosphate (Pi) through the oxidative phosphorylation. Evaluation of drug-induced mitochondrial toxicity has become increasingly important since mitochondrial dysfunction has recently been implicated in numerous diseases including cancer and diabetes mellitus. Mitochondrial functions have been monitored via oxygen consumption, mitochondrial membrane potential, and more importantly via ATP synthesis since ATP synthesis is the most essential function of mitochondria. Various analytical methods have been employed to investigate ATP synthesis in mitochondria, including high performance liquid chromatography (HPLC), bioluminescence technique, and pH measurement. However, most of these methods are based on destructive analysis or indirect monitoring through the enzymatic reaction. Infrared absorption spectroscopy (IRAS) is one of the useful techniques for real-time, label-free, and direct monitoring of biological reactions [1,2]. However, the strong water absorption requires very short path length in the order of several micrometers. Transmission measurements with thin path length are not suitable for mitochondrial assays because solution handlings necessary for evaluating mitochondrial toxicity, such as rapid mixing of drugs and oxygen supply, are difficult in such a narrow space. On the other hand, IRAS in the multiple internal reflection (MIR) geometry provides an ideal optical configuration to combine solution handling and aqueous-phase measurement. We have recently reportedon a real-time monitoring of drug-induced necrotic and apoptotic cell death using MIR-IRAS [3,4]. Clear discrimination between viable and damaged cells has been demonstrated, showing a promise as a label-free and real-time detection for cell-based assays. In the present study, we have applied our MIR-IRAS system to mitochondria-based assays by monitoring ATP synthesis in isolated mitochondria from rat livers. Mitochondrial ATP synthesis and hydrolysis were in situ monitored with MIR-IRAS, while dissolved oxygen level and solution pH were simultaneously monitored with O2 and pH electrodes, respectively. It is demonstrated that ATP synthesis and hydrolysis can be monitored by the IR spectral changes in phosphate groups in adenine nucleotides and MIR-IRAS is useful for evaluating time-dependent drug effects of mitochondrial toxicants.

• Korean journal of food and cookery science
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• v.31 no.5
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• pp.551-556
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• 2015

Iksan370 is a long-spike wheat developed by the Rural Development Administration yielding excellent features components such as cold resistance, disease resistance, and viviparous germination. The physicochemical and material properties of the raw wheat and milled flour of Iksan370 were analyzed to derive its appropriate uses. The raw wheat of Iksan370 showed high contents of ash and proteins at 1.71% and 13.7%, respectively. Its test weight of 763.0 g/L was similar to those of other varieties and its 1,000 kernel weight was high at 45.38 g. The milled flour of Iksan370 had an ash content of 0.45%, which corresponds with a class 1 flour, and its protein content is 12.18%, corresponding with strong flour. The damaged starch was 5.41%, which was lower than that of other varieties. The average grain size was $70.67{\mu}m$ and the grain distribution was at the level of a typical hard wheat. In the farinogram, the water absorption was 58.63%, which corresponded to the level of medium flour. The development time was 7.00 minutes, which was significantly lower than those of Jokyung and Keumkang. The degree of softening was 67.00 BU, similar to those of Yunbaek and Baekjoong. Among the physico-chemical characteristics, the high protein content and typical hard wheat grain distribution of Iksan370 were similar to those of strong wheat, usually used for bread making. However, in the farinogram, the dough development time was short and the degree of softening was high. As a result, Iksan370 was expected to have poor breadmaking properties and a small volume of the final bread product due to insufficient dough durability. On the other hand, Iksan370 showed the highest maximum gelatinization viscosity at 864.00 BU. Therefore, Iksan370 is expected to show glutinous texture when used for noodles and its flour appears to be appropriate for frying powders as well.

• Journal of Korean Medicine Rehabilitation
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• v.21 no.4
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• pp.13-22
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• 2011

Objectives : This Study was performed to evaluate the effects of Scutellariae Radix(SR) water-extract on the tissue and neuronal apoptosis of the spinal cord injury(SCI). Methods: SCI was induced by mechanical contusion following laminectomy of 10th thoracic vertebra in Sprague-Dawley rats. SR was orally given once a day for 7 days after SCI. Neuronal apoptosis was examined with terminal deoxynucleotidyl transferase-mediated dUTPnick-end labeling(TUNEL) assay. Bax (Bcl-2-asociated X protein), Bcl-2(B-cell blastoma 2), c-Fos(FBJ osteosarcoma oncogene) expressions were examined using immuno-histochemistry. Individual TUNEL and immuno-labeled cells expressing Bax, Bcl-2 and c-Fos were counted on the same level in peri-damaged region and in ventral horn. Results: 1. SR significantly reduced number of TUNEL labeled apoptotic cells induced by the spinal cord contusion injury. 2. SR significantly reduced Bax positive cells expression on the motor neuron in the ventral horn induced by the spinal cord contusion injury. 3. SR strengthened Bcl-2 expression on the motor neuron in the ventral horn induced by the spinal cord contusion injury. 4. SR reduced c-Fos expression on the motor neuron in the ventral horn induced by the spinal cord contusion injury. Conclusions : These results suggest that SR plays an inhibitory role against neuronal apoptosis and has significant effects for locomotor disfunction induced by SCI.

• Journal of Korean Society of Forest Science
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• v.89 no.2
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• pp.145-160
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• 2000

This study is focused to the national park of Korean typical Sea Hally$\check{o}$ Haesang, and its visual resources and practiced inspect course by the way of suppositions and tests, to show the visual resource management objectively, and that of qualitative basic data. Accordingly by measuring the physical amount spatial structure with the visual amount originated from the Mesh Analyzing Method and the Visual Preference from the Scenic Beauty Estimation(S.B.E.) method and analyzed the valuation of the visual resource by Iverson method. Spatial image structure measured by Semantic Differential(S.D.) Scale was shown through the factor analysis algorithm for the analyzing psychological amount and examined the flowing out of decisive factor and the objective importance related to the mutual factors by appling the measurement of the visual quality. As a national Park, the visual factors that have natural landscape harmonized with forest, sky, surface of the water, curious stones and rocks, and temples should be escalated their values affirmatively so as to be the scenery of pointed direction and enjoyable, and it is of more needed for visual resource and its' controlling technique to make artificial structures more intentional planning and systemical setting. When we are viewing the improvement for the national park along with the visual resource management, reasonable level of development is needed, because when men interference surpass plantations and leasts will be damaged and the quality of natural landscape can be lowered, so it is needed to set up a management end, tangibly or clearly; and it is permitted limit coming and going ablably by accounting the suitable number for availing. But the controling end should be set in every level, positive management, very actively within the permissive varcability. It is the main business for the national park to prevent the damage from human for their gay life or to prevent the damage of a land carpet, and to restorate for the visual resource management.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.36 no.3
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• pp.75-87
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• 2018

The purpose of this study is to investigate conservation and management methods of the Joseon Royal Tombs under the Eastern District Management Office. Through the literature survey, we understood the process of change of Joseon royal tombs, and through field surveys and interviews, we understood the status of the interior and the surrounding area. In this process, topography, land use and flow of human traffic, architecture and stone objects, water system, historical forests, and facilities were set as the main evaluation indicators. Urbanization has damaged the original terrains of Royal Tombs as national roads, buildings and facilities have constructed in the inner and outer area of Joseon Royal Tombs. Construction of underground passage, land purchase, relocation and demolition of the buildings are required for the conservation of the Royal Tombs area, and then it is necessary to recover the original terrain. In the case of land use and pathways, there are many disconnection of the original ritual circulation, they should be maintained to remind the sacred atmosphere of the royal tomb. And It is necessary to collect accurate information on the lost buildings and stoneworks through literature survey and excavation investigation, and that investigations should be lead to the exposure or restoration of the ruins. Historical forests require periodic and ongoing monitoring and management, and it is necessary to establish new entrance area and appropriate facilities following the long-Term conservation and management plan. These plans should be classified into short, medium and long-Term projects according to urgency and securing financial resources with a long perspective to implement continuous and systematic projects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.11
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• pp.1571-1576
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• 2012

The purpose of this study was to investigate the physicochemical and pasting properties of long-term stored domestic and imported rice supplied for food processing from government-controlled public rice stocks. Goamibyeo, which was bred for processing and harvested in 2011, was selected as the control rice for comparison. Rice was dry-milled, and the amylose contents of stored rice were 12~13%. Stored rice kernels were significantly harder than those of control, whereas damaged starch content and water absorption of flour were not significantly different from those of control. Overall, long-term stored domestic and imported rice showed high peak viscosities along with high viscosities of both hot and cold pastes. Both imported and domestic rice demonstrated insufficient properties for making 100% flat rice noodles or bread. They showed the greatest shrinkage during cooling after baking. Although their levels of cooking loss were less than that of control, stored rice showed a less elastic and softer cooked noodle texture compared to control flour.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.24 no.5
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• pp.305-318
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• 2012

Although the existing performance-based design method for the vertical breakwater evaluates an average sliding distance during an arbitrary time, it does not calculate the probability of the first occurrence of an event exceeding an allowable sliding distance(i.e. the first-passage probability). Designers need information about the probability that the structure is damaged for the first time for not only design but also maintenance and operation of the structure. Therefore, in this study, a time-dependent reliability design method based on a stochastic process is developed to evaluate the first-passage probability of caisson sliding. Caisson sliding can be formulated by the Poisson spike process because both occurrence time and intensity of severe waves causing caisson sliding are random processes. The occurrence rate of severe waves is expressed as a function of the distribution function of sliding distance and mean occurrence rate of severe waves. These values simulated by a performance-based design method are expressed as multivariate regression functions of design variables. As a result, because the distribution function of sliding distance and the mean occurrence rate of severe waves are expressed as functions of significant wave height, caisson width, and water depth, the first-passage probability of caisson sliding can be easily evaluated.

• Journal of Wetlands Research
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• v.20 no.1
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• pp.20-26
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• 2018

The purpose of this study is to construct a GIS / DB by grasping a small but ecologically valuable village wetland distribution, and to propose conservation management and wise use plan. The study area is Seocheon-gun, a typical farming village. Firstly, based on the digital topographical map (1:5,000), the Arc-GIS tool was used to identify the provisional(draft) village wetlands. In addition, for the management of village wetlands, wetlands with an area of more or less than $625m^2$ each were derived and according to ecological regions study area was classified into urban areas, inland areas and coastal areas. And finally, according to the wetland identifying indicators, the village wetlands were identified as the final village wetlands through indoor and field trips. The results of the study show that there are 570 village wetlands in Seocheon - gun province, which are 74 in urban areas, 220 in inland areas, and 276 in coastal areas. The case study for village wetland identification was conducted in one out of two urban areas (Seocheon - eup), two of four coastal areas (Biin - myeon and Seo - myeon), and three of seven inland areas (Masan - myeon, Hansan - myeon, and Sicho - myeon). The distribution of village wetlands was found mainly to be a village wetland with an area of less than $625m^2$. In addition, compared with inland areas, the discrimination rate of village wetlands in coastal areas and urban areas was relatively low, indicating that inland areas were still less disturbed, and land use in urban areas and coastal areas is changing rapidly. Especially, land with less awareness such as village wetlands is relatively easily damaged, and management strategy is urgent.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.4
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• pp.1082-1091
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• 2003

To elucidate the neuroprotective effect of Kamijihwang-hwan(KSH) on nerve cells damaged by hypoxia, the cytotoxic effects of exposure to hypoxia were determined by XTT, NR, MTT and SRB asssay. The activity of catalase and SOD was measured by spectrophometry, and TNF-α and PKC activity was measured after exposure to hypoxia and treatment of Kamijihwang-hwan(KSH) water extract(KJHWE). Also the neuroprotective effect of KJHWE was researched for the elucidation of neuroprotective mechanism. The results were as follows ; Hypoxia decreased cell viability measured by XTT, NR assay when cultured cerebral neurons were exposed to 95% N2/5% CO₂ for 2～26 minutes in these cultures and KJHWE inhibited the decrease of cell viability. H₂O₂ treatment decreased cell viability measured by MTT, and SRB assay when cultured cerebral neurons were exposed to 1-80 uM for 6 hours, but KJHWE inhibited the decrease of cell viability. Hypoxia decreased catalase and SOD activity, and also TNF-α and PKC activity in these cultured cerebral neurons, but KJHWE inhibited the decrease of the catalase and SOD activity in these cultures. Hypoxia triggered the apoptosis via caspase activation and internucleosomal DNA fragmentation. Also hypoxia stimulate the release of cytochrome c form mitochondria. KJHWE inhibited the apoptosis via caspase activation induced by hypoxia. From these results, it can be suggested that brain ischemia model induced hypoxia showed neurotoxity on cultured mouse cerebral neurons, and the KJHWE has the neuroprotective effect in blocking the neurotoxity induced by hypoxia in cultured mouse cerebral neurons.

• Nutritional Sciences
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• v.9 no.4
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• pp.267-272
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• 2006

The effect of methanol extract from Agaricus blazei Murill on the hepatotoxicity was investigated $CCl_4$ is one of the oldest and most widely used toxins for the induction of hepatic damages and fibrosis in experimental animals. In this study, male Sprague-Dawley(SD) rats were randomly divided into 6 groups of the control(C), $CCl_4(T),\;CCl_4$ and high fat group(TL) with matching sub-groups of Agaricus blazei Murill extract-fed groups of CA, TA and TLA. Methanol extracts of Agaricus blazei Murill were fed 50 mg/kg B.W daily via drinking water. A 1.2 mL of $CCl_4/kg$ body weight was administered by oral intubation twice a week for total of six times. The levels of total-cholesterol, TG, LDL and LDL-phospholipids were elevated by $CCl_4$ treatment as compared to the control(C). However, Agaricus blazei Murill methanol extract feeding in the group of TA and TLA significantly(p<0.05) decreased TG by 53.1 % and 17.9% compared to the internal control of T and TL, respectively. Triglyceride of TL was increased by 3.33 times(p<0.05) compared to the control(C) with $CCl_4$ and high fat administration from 3.78 mg/g to 12.60 mg/g liver. The extract(CA) also reduced kidney weight compared to the control(C). With the administration of high fat and $CCl_4$(TLA), the extract reduced the organ weight of both liver and kidney and further, significantly reduced TG, total cholesterol and GTP activity. Hepatoprotective effects of Agaricus blazei Murill on GOT, GPT, AP and LDH activities were enhanced by the extract feeding. Electronmicrograph showed that $CCl_4$ deteriorated the structure of cytoplasmic matrix with its uneven distribution. However, the extract reconstituted the damaged cytoplasm and stimulated mitochondriogenesis. The above results suggest that Agaricus blazei Murill may have a possible protective effect against chemically induced liver damage and further help to reduce the symptoms of fatty liver.