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Inhibitory Effects of Euphorbia supina Rafin on the Production of Pro-Inflammatory Mediator by LPS-Stimulated RAW 264.7 Macrophages (LPS로 활성화된 RAW 264.7 대식세포에서 애기땅빈대(Euphorbia supina Rafin)의 염증매개물질 억제효과)

  • Park, Sung-Chul;Son, Dae-Yeul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.4
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    • pp.486-492
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    • 2011
  • This study was designed to evaluate the effect of hot water extract (ESW) and 70% ethanol extract (ESE) from Euphorbia supina Rafin on LPS-stimulated inflammatory response in RAW 264.7 macrophages. Upon investigation at concentrations up to $1000\;{\mu}g$/mL, ESW and ESE did not have any cytotoxic effects on RAW 264.7 macrophages. ESW induced inhibition of 21.6%~54.8% of nitric oxide (NO) production at 100~1000${\mu}g$/mL, and $PGE_2$ production was inhibited up to 25.7%~38.2% at 250~1000${\mu}g$/mL, proportional to the ESW concentrations. ESW induced inhibition of 66.1% and 54.3% of IL-6 production at 250 and $1000\;{\mu}g$/mL, respectively. ESE (100~1000${\mu}g$/mL) induced inhibition of 38.3%~77.5% of NO, 40%~94.7% of $PGE_2$, and 43.9%~89.4% of IL-6 production, proportional to the ESE concentrations. Only 44.1% of IL-10 production was inhibited at a concentration of $500\;{\mu}g$/mL. ESE induced an increase in TNF-${\alpha}$ production at a concentration of 100 and $250\;{\mu}g$/mL, whereas at high concentrations (500 and $1000\;{\mu}g$/mL), ESE induced inhibition of 19.2% and 92.4% of TNF-${\alpha}$ production, respectively. In conclusion, concentrations of more than $500\;{\mu}g$/mL ESE demonstrated effective immune-modulating activity through inhibition of NO, $PGE_2$, IL-6, IL-10, or TNF-${\alpha}$ production, as it relates to the macrophage's immuno-activity; therefore, ESE has potential as a good candidate substance for reduction of inflammatory responses.

Effect of Fermented Cucumber Beverage on Ethanol Metabolism and Antioxidant Activity in Ethanol-treated Rats (오이 발효음료가 만성적으로 에탄올을 급여한 흰쥐의 에탄올 대사와 항산화방어계에 미치는 영향)

  • Lee, Hae-In;Seo, Kwon-Il;Lee, Jin;Lee, Jeom-Sook;Hong, Sung-Min;Lee, Ju-Hye;Kim, Myung-Joo;Lee, Mi-Kyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.8
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    • pp.1099-1106
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    • 2011
  • Cucumber fermentation has been used as a means of preservation. This study was performed to investigate the effects of fermented cucumber beverage (CF) containing beneficial materials for an ethanol hangover based on Hovenia dulcis (SKM) on ethanol-induced hepatotoxicity. Male Sprague-Dawley rats were randomly divided into three groups: ethanol control, ethanol plus SKM, and ethanol plus CF+SKM. SKM or CF+SKM was orally administered at a dose of 7 mL/kg body weight once per day for 5 weeks. Control rats were given an equal amount of water. CF+SKM significantly lowered plasma ethanol levels, whereas SKM tended to decrease the levels compared to the control. Both SKM and CF+SKM significantly lowered the plasma acetaldehyde levels and serum transaminase activities compared to those in the control. SKM and CF+SKM did not affect hepatic alcohol dehydrogenase activity; however, it significantly inhibited cytochrome P450 2E1 (CYP2E1) activity. Hepatic aldehyde dehydrogenase (ALDH) activity was significantly higher in the SKM and CF+SKM groups than that in the control group. Plasma acetaldehyde concentration was significantly correlated with hepatic CYP2E1 (r=0.566, p<0.01) activity and ALDH (r=-0.564, p<0.01) activity. Hepatic superoxide dismutase and catalase activities as well as glutathione content increased with the SKM and CF+SKM administration, whereas lipid peroxide content decreased significantly. Furthermore, SKM and CF+SKM lowered plasma and hepatic lipid content and lipid droplets compared to those in the control group. These results indicate that SKM and CF+SKM exhibit hepatoprotective properties partly by inhibiting CYP2E1 activity, enhancing ALDH activity and stimulating the antioxidant defense systems in ethanol-treated rats.

Preparation and Quality Characteristics of Seasoned and Dried Fish Slice Products Using Rainbow Trout (Onchorhynchus mykiss) (송어 육포의 제조와 품질 특성)

  • Heu, Min-Soo;Kim, Hyung-Jun;Ham, Joon-Sik;Park, Shin-Ho;Kim, Hye-Suk;Kang, Kyung-Tae;Jee, Seung-Joon;Lee, Jae-Hyoung;Kim, Jin-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.3
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    • pp.348-356
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    • 2008
  • For the effective use of rainbow trout, the seasoned and dried rainbow trout slice (SR) was prepared and its characterization was compared with the commercial skipjack tuna (CSS) and pork products (CSP) by determining chemical components and sensory evaluation. The moisture content of SR, 20.5%, was lower, and the lipid content of SR, 7.5%, was higher than those of commercial products, CSS and CSP. The protein content of SR (41.4%) was higher than that of CSS (28.6%), but lower than that of CSP (50.1%). The water activity of SR was 0.654, which was lower than those of CSS and CSP, 0.724 and 0.771, respectively. According to the results, the color of SR was lower in lightness and redness and higher in browning index and ${\Delta}$E value than that of commercial products. The texture of SR was harder than that of CSS, but softer than that of CSP. According to the result of taste value, the taste of SR was stronger than that of CSP, while weaker than that of CSS. The mineral (Ca, P, and Fe), total amino acid contents, and the n-3/n-6 of SR showed significant levels in nutrition and health functional senses.

Effect of Conidial Number and Nutrition on the Germination of Conidia in Colletotrichum dematium f. sp. capsicum Causing Red Pepper Anthracnose (분생포자수(分生胞子數) 및 영양상태(營養狀態)가 고추 탄저병균(炭疽病菌)(Colletotrichum dematium f. sp. capsicum)의 분생포자(分生胞子) 발아(發芽)에 미치는 영향(影響))

  • Chung, Bong-Koo;Lee, Sang-Bum
    • Korean journal of applied entomology
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    • v.25 no.1 s.66
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    • pp.41-46
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    • 1986
  • Exogenous factor and nutrients affecting for conidial germination of Colletetrichum dematium f. sp. capsicum causing red pepper anthracnose were studied by slide germination test. Optimum temperature of conidial germination was at $28^{\circ}C$, ranging 15 to $35^{\circ}C$. Optimum pH was at 5.5, ranging 4.5 to 8.0, and more than 90% of relative humidity (RH) was optimum. Poor conidial germination of the fungus was observed on sterile distilled water, but potato sucrose broth (PSB), red pepper fruit broth (RPFB), green pepper fruit broth (GPFB) and pepper leaf broth (PLB) furnished a satisfactory nutrients for conidial germination. Exogenous supply of carbon and nitrogen sources were essential for conidial germination, while potassium, phosphorous and sulfur were not evident as that for carbon and nitrogen. Soluble starch was the most suitable as a carbon source for conidial germination and followed by D-glucose, D-galactose and lactose in that order. Maximum germination was attained in the $1{\times}10^4$ conidia per ml. Germination was decreased with increment of conidial concentration, and in the density of $5{\times}10^4$ conida per ml, germination was nearly supipressed. It suggested existing a self-inhibitor. Non-washed conidia germinated more than washed conidia, and conidial germination was also gradually decreased by increasing conidial density.

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The mechanism of black core formation (블랙코어 형성 메커니즘)

  • Park Jiyun;Kim Yootaek;Lee Ki-Gang;Kang Seunggu;Kim Jung-Hwan
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.15 no.5
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    • pp.208-215
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    • 2005
  • The 10mm diameter aggregates made of clay, carbon and $Fe_2O_3$ were prepared to investigate the mechanism of black core formation. The specific gravity, absorption rate, percent of black core area, fracture strength, total Fe analysis, and XRF were measured at various compositions, sintering temperatures, sintering times, sintering atmospheres, and sintering methods. Small addition of $Fe_2O_3$ did not affect physical properties of the aggregates; however, the percent of black core area increased with increasing carbon contents and increasing sintering temperature. Specific gravity of the aggregates decreased and the water absorption ratio increased with increasing percent of black core area. The aggregates sintered at oxidation atmosphere showed clear border between shell and black core area. Hence, the aggregates sintered at reduction atmosphere showed only black core area in the cross-section of the aggregates. The specific gravity of the aggregates sintered at reduction atmosphere increased with increasing carbon contents and that was the lowest of all comparing other aggregates sintered at different atmospheres. Adsorption rate increased with increasing carbon contents at all atmospheres. The fast sintered aggregates showed lower specific gravity, higher absorption rate, and more black core area than the normally sintered aggregates. It was turned out that the aggregates having more black core area showed higher fracture strength than that of aggregates with no black core area. From the total Fe analysis, the concentration of Fe and FeO was higher at black core area than at shell. Because the concentration of $Fe_2O_3$ in the shell was higher than other area, the color of the shell appeared red. It was also turned out from the XRF analysis that carbon was exist only at black core area.

Change Detection of land-surface Environment in Gongju Areas Using Spatial Relationships between Land-surface Change and Geo-spatial Information (지표변화와 지리공간정보의 연관성 분석을 통한 공주지역 지표환경 변화 분석)

  • Jang Dong-Ho
    • Journal of the Korean Geographical Society
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    • v.40 no.3 s.108
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    • pp.296-309
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    • 2005
  • In this study, we investigated the change of future land-surface and relationships of land-surface change with geo-spatial information, using a Bayesian prediction model based on a likelihood ratio function, for analysing the land-surface change of the Gongju area. We classified the land-surface satellite images, and then extracted the changing area using a way of post classification comparison. land-surface information related to the land-surface change is constructed in a GIS environment, and the map of land-surface change prediction is made using the likelihood ratio function. As the results of this study, the thematic maps which definitely influence land-surface change of rural or urban areas are elevation, water system, population density, roads, population moving, the number of establishments, land price, etc. Also, thematic maps which definitely influence the land-surface change of forests areas are elevation, slope, population density, population moving, land price, etc. As a result of land-surface change analysis, center proliferation of old and new downtown is composed near Gum-river, and the downtown area will spread around the local roads and interchange areas in the urban area. In case of agricultural areas, a small tributary of Gum-river or an area of local roads which are attached with adjacent areas showed the high probability of change. Most of the forest areas are located in southeast and from this result we can guess why the wide chestnut-tree cultivation complex is located in these areas and the capability of forest damage is very high. As a result of validation using a prediction rate curve, a capability of prediction of urban area is $80\%$, agriculture area is $55\%$, forest area is $40\%$ in higher $10\%$ of possibility which the land-surface change would occur. This integration model is unsatisfactory to Predict the forest area in the study area and thus as a future work, it is necessary to apply new thematic maps or prediction models In conclusion, we can expect that this way can be one of the most essential land-surface change studies in a few years.

A Study on the Utilization Status of the Interior Landscape Plants in Large Buildings in Seoul (우리나라 실내조경식물의 활용실태에 관한 연구 -서울지역 대형건물의 Lobby를 중심으로-)

  • 박상헌;심경구
    • Journal of the Korean Institute of Landscape Architecture
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    • v.17 no.1
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    • pp.43-54
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    • 1989
  • In recent years plants have increasingly become in inferior part of the interior designing of large buildings. In 28 large buildings selected at random in Seoul City have been subjected to studies on utilization status of fille interior landscape plants. The results obtained were as follows. 1. Plant class found in Interior of large buildings were 59 class, especially Dracaena were the most predominent plants among them, and then comes Monstera, Phiodendron, Schefflera, and Washingtonia in that order. 2. The most abundant ornamental plant cultivars were Monstera adansoii(Monstera perutusa cv.), and then comes Schefflera arboricora(Schefflera Hong Kong cv.), Washingionia rubusta (Washingtonia palm). Rhapis excelsa, and Diffenbachia(Hawaii Snow cv.) in that order. 3. Foliage plants among the interior landscape plants showed 99.5 percent and Woody plants and Bonsai plants showed each 0.4, 0.1 percent. 4. Climbing foliage plants found in 28 large building observed For the present study were 9 cultivar, and the most abundant plants were Hedera (pittsburgh cv.) and Epipremnum (Marble Queen cv.) pl anted to clustered planting. 5. A majority of plants used to plastic pot, and most of 71an1s place on the floor. 6. Height of plants ranging less then 50cm were more frequently found, but ranging from 50cm to 2.0m were 4.3 percent and the most predominent height of plants were Canariensis showed 4m in the large buildings. 7. Interior light intensity in large building were shortage, therefore artificial lamp for formal growing needed to be equipped. 8. Purpose growth of interior plants were to in order visitors. 9. 7he superintendents prefered to used pot-stand. 10. The most important environment factor for plant growing were light, humidity, and water.

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Analysis of User′s Satisfaction to the Small Urban Spaces by Environmental Design Pattern Language (환경디자인 패턴언어를 통해 본 도심소공간의 이용만족도 분석에 관한 연구)

  • 김광래;노재현;장동주
    • Journal of the Korean Institute of Landscape Architecture
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    • v.16 no.3
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    • pp.21-37
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    • 1989
  • Environmental design pattern of the nine Small Urban Spaces at C.B.D. in City of Seoul are surveyed and analyzed for user's satisfaction and behavior under the environmental design evaluation by using Christopher Alexander's Pattern Language. Small Urban Spaces as a part of streetscape are formed by physical factors as well as visual environment and interacting user's behavior. Therefore, user's satisfaction and behavior at the nine Urban Small Spaces were investigated under the further search for some possibilities of application of those Pattern Languages. A pattern language has a structure of a network. It is used in sequence, going through the patterns, moving always from large patterns to smaller, always from the ones which create comes simply from the observation that most of the wonderful places of the city were not blade by architects but by the people. It defines the limited number of arrangements of spaces that make sense in any given culture. And it actually gives us the power to generate these coherent arrangement of space. As a results, 'Plaza', 'Seats'and 'Aecessibility' related design Patterns are highly evaluated by Pattern Frequency, Pattern Interaction and their Composition ranks, thus reconfirm Whyte's Praise of urban Small Spaces in our inner city design environments. According to the multiple regression analysis of user's evaluation, the environmental functions related to the satisfaction were 'Plaza', 'Accessibility' and 'Paving'. According to the free response, user's prefer such visually pleasing environmental design object as 'Waterscape' and 'Setting'. In addition to, the basic needs in Urban Small Spaces are amenity facilities as bench, drinking water and shade for rest.

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Roots Growth Characteristics of Zelkova serrata Makino. after Replanting in the Reclaimed Land from the Sea - On the Root Structure and Spatial Distribution of Fine Root Phytomass - (임해매립지의 느티나무 식재 이후 뿌리 생장특성 -뿌리구조 및 세근의 공간적 분포를 중심으로-)

  • Kim, Do-Gyun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.35 no.5
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    • pp.46-55
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    • 2007
  • This study was carried out to analyze both the root structure and the fine root phytomass of the vertical and horizontal distribution of Zelkova serrata Makino. which was transplanted in the reclaimed land from the sea in Gwangyang, Jeonnam, South Korea. The base ground was reclaimed land from the sea. $Z_1$ of the planting ground was filled to a $100{\sim}150cm$ thickness with the improved soil instead of the reclaimed soil from the sea, $Z_2$ of the planting ground was covered to a $20{\sim}30cm$ thickness with the improved soil and $Z_3$ of the planting ground was mounded to 120cm thickness with the improved soil on the reclaimed land from the sea. In addition, $Z_4,\;Z_5\;and\;Z_6$ of the planting grounds were at the large-sized mound on the reclaimed land from the sea. $Z_4$ of the planting ground was located at the lowest level, $Z_5$ planting ground was located at the slope and $Z_6$ planting ground was located at the top of the large-sized mound. The large-sized mounds contain 3 layers, the base layer was reclaimed land from the sea and the second layer was mounded to a $200{\sim}300cm$ thickness with the desalinized soil from the sea on the base layers and the finally layers were mounded to a $80{\sim}120cm$ thickness with improved soil on the second layer. The planting grounds $Z_3,\;Z_4,\;Z_5\;and\;Z_6$ developed roots such as tap roots, lateral roots and heart roots. However, in $Z_1\;and\;Z_2$ roots development were inhibited. The fine-root phytomass of the 6 planting ground types was as follows: $113.5g\;DM/m^2$ for $Z_5$, $105.5g\;DM/m^2$ for $Z_4$, $88.3g\;DM/m^2$ for $Z_3$, $81.0g\;DM/m^2$ for $Z_6$, $73.0g\;DM/m^2$ for $Z_2$, $43.3g\;DM/m^2$ for $Z_1$. The vertical distribution of the fine root phytomass decreased from the upper to the deeper soil profiles in the 6 mound types. The fine root phytomass was $43.3{\sim}71.8%$ in a $0{\sim}20cm$ thickness of soil layer and it decreased according to the distance from the nearest trees. The root growth in the improved soil was better than in the reclaimed soil from the sea. However, root growth decreased more in the disturbed soils even though the planting grounds contained the improved soils. The retarded development of roots and the spatial distribution patterns of the fine root phytomass were closely connected to the reclaimed soil from the sea. In the disturbed soil, the soil hardness and alkalic cation($Na^+,\;K^+,\;Ca^{2+},\;Mg^{2+}$). were high and the soil water was lacking. We suggest that the construction of planting grounds and the improvement of bad soil are necessary for the proper and effective growth of landscaping plants.

Prior Eco-preserve Zoning through Stream Ecosystem Evaluation on Dam Basin -A Case of Yongdam-dam Watershed, Jeollabukdo Province- (댐유역 하천생태계평가를 통한 생태보전우선지역설정 -용담다목적댐 유역을 사례로-)

  • Lim, Hyun-Jeong;Lee, Myung-Woo
    • Journal of the Korean Institute of Landscape Architecture
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    • v.39 no.2
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    • pp.103-112
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    • 2011
  • The purpose of this study is to specify the prior eco-preserve zone by establishing the eco-landscape unit on the stream corridor and evaluating the stream ecosystem in the dam basin. The fundamental ecological data was surveyed and collected through "the ecosystem project on Yongdam multipurpose dam watershed" from 2008 to 2009. The Yongdam Dam Watershed has several streams, Jujacheon, Jeongjacheon and Guryangcheon, of which the area is $930km^2$, stretching to Jinangun, Jangsugun and Mujugun Jellabukdo. In spite of being used for drinking purpose, the dam water quality and ecosystem is threatened by in-watershed pollution produced by development, golf course grounds and sports complex, etc. The landscape unit of stream corridor was zoned across by 250m, 500m, and 750m from the vicinity line of stream, which was decided to the accuracy of mapping and surveying. Types of evaluation are the Stream Corridor Evaluation(SCE) and the Vegetated Area Evaluation(VAE). In the process of SCE, several indices were analysed, fish species diversity, species peculiarity, and stream naturality. Indices for VAE were forest stand map, vegetation protection grade, species diversity and peculiarity for wild bird and mammal life. The importance of the ecological items is categorized into three levels and overlapped for specifying the prior preserve zone. The area at which legally protecting species appeared is categorized as absolute preserve area. This study might be meaningful for proposing the evaluation process of a stream corridor ecosystem, which can synthesize a lot of individual ecological surveys. We hope further research will be actively performed about the ecotope mapping which is based on a individual wildlife territory and habitats and also their relationships.