• Title/Summary/Keyword: washing times

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A study on production of early pregnancy diagnostic kit in cattle 1. Production of polyclonal antibody to progesterone and removal of anti-bovine serum albumin antisera (소의 조기임신진단 kit의 개발 1. Progesterone의 항체생산(抗體生産) 및 항(抗) BSA항체(抗體)의 제거)

  • Kang, Chung-boo;Lee, Hyo-jong;Choe, Sang-yong
    • Korean Journal of Veterinary Research
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    • v.31 no.2
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    • pp.217-222
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    • 1991
  • Most progesterone enzyme immunoassays(EIA) are used liquid phase double-antibody separation. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants. Because of there several problems, we were prompted to develop an effective EIA system by the use of higher titer of progesterone antiserum free of anti-bovine serum albumin antibodies (anti-BSA). The results obtained were as follows. 1. The antibody of progesterone antiserum was high as $1.5{\times}10^5$. 2. Percent activity bound of progesterone antiserum was about 77 at a dilution to $5{\times}10^3$ times. 3. Progesterone antiserum was contained a large amount of anti-BSA antibodies. 4. The anti-BSA was completely absorbed by using of polymerised BSA. 5. The molecular weight of albumin polymer (polymerised BSA) obtained by using 2.5% glut. araldehyde was $5{\times}10^5$.

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Synthesis and Characterization of Water-soluble Polyamine Durable Antistatic Agent (수용성 폴리아민 내구성 대전방지제의 합성과 그의 특성화)

  • Kim, Seung-Jin;Kim, Han-Ku;Keun, Jang-Hyoun;Park, Hong-Soo
    • Applied Chemistry for Engineering
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    • v.5 no.3
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    • pp.466-477
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    • 1994
  • PEG 600-diglycidyl ethers(PDE) were synthesized using $BF_3$ catalyst by the reaction of epichlorohydrin and PEG 600 which is known to have the hygroscopicity, softening property, and antistatic property. Water-soluble long chain polyamines(PDET) were synthesized by coreaction of PDE and triethylenetetramine which is high conductive aliphatic amine curing agent. To prevent the gelation of the PDET and increase the water-solution stability, water-soluble quaternary ammonium polyamines were synthesized by cationation of PDET with acetic acid. Antistatic agents PDET-2A, PDET-5A, PDET-6A, PDETA-2A and PDETA-4A were prepared by the mixing of PDET-2, PDET-5, PDET-6, PDETA-2 and PDETA-4 with water. Synthesized antistatic agents were treated on PET textiles with and without resin. Then surface electrical resistivity and half life characteristics value were tested. As the results there were no remarkable decreasing changes in antistatic abilities of the textiles treated with PDETA-2A and PDETA-4A after 50 times washing. So PDETA-2A and PDETA-4A were proved to be durable antistatic agents. Surface electrical resistivity of the textiles treated with PDETA-2A and PDETA-4A before washing were $1{\times}10^7{\Omega}$ and $2{\times}10^7{\Omega}$, respectively, and half life characteristics values were 0.8sec and 1.1sec, respectively. Therefore PDETA-2A and PDETA-4A were proved to be good antistatic agents.

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Modification of Dyeing Property of Wool Fabrics by UV Irradiation (UV조사에 의한 양모직물의 염색성 개질)

  • 전영실;남성우;김인회
    • Textile Coloration and Finishing
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    • v.16 no.1
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    • pp.10-17
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    • 2004
  • Wool fabrics were treated with deep UV for 10 to 30min and then dyed with C.I. Acid Yellow 99, C.I, Acid Red 57 and C.I. Acid Blue 62. FT-IR and XPS have been used to prove the chemical composition of wool fiber surfaces. The mechanical property and colorfastness were also studied. The intencities of the peaks of C=O group at $1700^cm^{-1}$ were increased with increasing irradiation time. Relative $O_{1s},\; N_{1s}\; and\; S_{2p}$ intensities increased considerably and oxygen was incorporated in the form of CO and COO on the fiber surface. The dye uptakes of wool fabrics dyed with three acid dyes were proportional to irradiation times and in inversely proportional to irradiation distances. It was found that the tensile strength of wool fabrics were gradually deteriorated with the UV irradiation times. The colorfastness, such as washing, light and rubbing, of UV irradiated wool fabrics dyed with acid dyes were good.

A study on isolated microorganisms from dental cast (석고 모형으로부터 분리 배양된 미생물에 관한 연구)

  • Choi, Ju-Young
    • Journal of Technologic Dentistry
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    • v.35 no.4
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    • pp.321-332
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    • 2013
  • Purpose: This study was examined the characteristics of bacteria isolated from the dental stone that is made ??in the dental laboratory. Methods: 104 dental stones samples were collected from the 4 dental laboratory. Characteristics of bacteria were investigated by microorganism isolation culture method using a Blood Tryptic Soy Agar(TSA) medium. Results: The detected various bacteria was confirmed as pathogenic bacteria, non-pathogenic bacteria and natural bacteria. The isolated bacterial number was confirmed $2.9{\times}10^3CFU$ and maxium bacterial number of $3.0{\times}10^4CFU$. Conclusion: Therefore, infection prevention education is required, it must be to live up the hand-washing and wear protective clothing to protect themselves when working in a dental laboratory.

Utilization of Alunite to Ceramic Raw Materials (III) (요업원료로서 명반석의 이용에 관한 연구(제III보) Mullite질내화재원료로의 이용)

  • 백용혁;최상흘
    • Journal of the Korean Ceramic Society
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    • v.11 no.3
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    • pp.33-38
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    • 1974
  • The possibility of the sources for the manufacture of mullite-rich refractories from the modified domestic alunite was studied. The modifying method of alunite studied were performed by calcination, wet ballmilling, and washing with water. For synthesis of mullite-rich refractories, the modified alunite with the addition of alumina and Fe2O3 as mineralizer was fired at 1350$^{\circ}$-155$0^{\circ}C$, and the following results were obtained: 1) The suitable firing temperature range was 1450$^{\circ}$-150$0^{\circ}C$, and adequate amounts of Al2O3 and Fe2O3 were below 30% and 3~4%, respectively. 2) Thermal expansion coefficient proportional to heating temperature was about 5$\times$10-6~10$\times$10-6cm/cm.deg. 3) The mineralogical compositions of the sintered specimens were found as mainly mullite and corundum.

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Determining the residual surfactants in the fruits and vegetable washed with detergent (세척제를 사용한 과일 및 채소의 세척단계별 계면활성제 잔류량)

  • Lee, So Jeong;Park, Na-Youn;Kho, Young Lim;Kim, Jung Hoan
    • Korean Journal of Food Science and Technology
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    • v.53 no.6
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    • pp.792-798
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    • 2021
  • This study was conducted to determine the washing conditions in which surfactants were not detected after washing fruits and vegetables with detergent. Residual surfactants were analyzed using LC-MS/MS. The analyzed surfactants were LAS, SLES, AOS, SLS, CAPB, and LDAO. The survey found that 14% of the respondents used detergent correctly according to the directions indicated. After soaking the samples with 0.2% detergent, the average residual surfactant content was 2.61%. Soaking under 1% and 5% of the detergent, 4.68-5.50% of surfactant remained in the sample. In cherry tomatoes, the surfactants were below the LOD after rinsing twice. However, lettuce was rinsed three times. These results suggest that when using detergent for washing fruits and vegetables, following the labelled usage and rinsing at least three times in 30 seconds could minimize residual surfactants.

Analysis of Roadside Fugitive Dust Generation Characteristics in Rural Areas Adjacent to Quarry Sites (채석단지 인근 농촌지역 도로 비산먼지 발생 특성 분석)

  • Seo, Hyo-Jae;Seo, Il-Hwan;Jung, Nam-Su;Park, Joo-Seok
    • Journal of Korean Society of Rural Planning
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    • v.30 no.3
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    • pp.1-8
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    • 2024
  • This study focused on quantifying fugitive dust levels around rural village adjacent near a stone quarry, specifically examining the influence of quarry operations and gravel transport vehicles on road dust dispersion. For this purpose, fugitive dust concentration monitoring was conducted to understand the impact of gravel transport vehicles operating through the quarry area on the generation of road dust. Gravimetric analysis indicated substantial increases in Total Suspended Particulate (TSP) concentration (5.8 times), PM-10 (3.6 times), and PM-2.5 (2.0 times) during quarry operations on the quarry transport road. Real-time monitoring during dump truck operation revealed average dust concentrations of TSP 566.8 ㎍/m3, PM-10 174.8 ㎍/m3, and PM-2.5 55.3 ㎍/m. This indicates a significant increase compared to non-operation periods, with TSP concentration rising by 5.8 times, PM-10 concentration by 3.6 times, and PM-2.5 concentration by 2.0 times. Significantly elevated dust levels were observed at the village entrance road and quarry transport road during quarry operations, with PM-10 exceeding regulatory limits by 2.2 times and PM-2.5 exceeding limits by 1.9 times on the quarry transport road. Especially, particles with a diameter greater than 10 ㎛ exhibited a meaningful increase during dump truck transportation. Considering these findings, the study recommends implementing dust mitigation measures, including the introduction of dust barriers, screens, and wheel washing facilities for quarry-bound dump trucks.

Verification of the HACCP System in School Foodservice Operations - Focus on the Microbiological Quality of Foods in Non-Heating Process - (학교급식소의 HACCP 시스템 적합성 검증(II) -비가열조리 공정을 중심으로-)

  • 전인경;이연경
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.7
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    • pp.1154-1161
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    • 2004
  • The objective of this study was to evaluate microbiological Quality of HACCP application in elementary school foodservice operations. Microbiological Quality of utensils and non-heated foods such as salad was measured two times at five elementary schools in Daegu. The two experimentations differed in that after the first experimentation employees were educated and trained on HACCP based sanitation standards with a goal of improving microbiological Quality of foodservice. Microbiological Quality, time, and temperature were evaluated at three critical control points (CCPs): washing and disinfecting, personal hygiene and non-cross contamination. Micro-biological Quality was assessed using 3M petrifilm to measure total plate count and coliform group. The first experimentation showed low microbiological Quality due to cross contamination of utensils and cooking gloves; high microbiological count of the garlic, powdered red pepper, and ginger; and not thoroughly washing and disinfecting vegetables. In the second experimentation, microbiological Quality was greatly improved by washing and thoroughly disinfecting raw ingredients and utensils, and using good personal hygiene. However, microbiological Quality of seasonings was still low. Immediate corrective actions were required in one of the foodservice operations that was assessed. These results strongly suggest that foodservice operations should address non-heated food Quality. It is essential to measure microbiological Quality regularly and continually train and retrain employees on hand washing and disinfecting raw ingredients. Further studies are needed to determine whether pathogens are present in raw vegetables and seasonings.

Elimination of Phenthoate Residues in the Washing and Cooking of Polished Rice (쌀의 취반 중 Phenthoate 농약 잔류분의 제거)

  • Kim, Nam-Hyung;Lee, Mi-Gyung;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.490-496
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    • 1996
  • This study was undertaken in order to elucidate the elimination of phenthoate residues by washing and cooking processes of rice which if the most important food crop in Korea. When contaminated rice was washed with distilled water three times, the removal rate of total phenthoate was 51%. The removal rate in the successive washings was 37.3% (wash filtrate 7.8%, wash sediment 29.5%) in the first, 14.3% (wash filtrate 6.2%, wash sediment 8.1%) in the second and 8.9% (wash filtrate 5.8%, wash sediment 3.1%) in the third washings. More than half of the residue was removed by the first washing and most residues were found in the sediment rather than in the filtrate of the rice washings. The residue rate of phenthoate after cooking by an electric rice cooker was 41%, indicating that the removal rate after cooking was 59%, because phenthoate is thermally stable at the cooking temperature. In conclusion, phenthoate residues contaminated in rice grains are grcatly removed in the washing process and it is desirable to wash the grains before cooking in order to decrease the hazards from pesticide residues such as phenthoate. Reduction factor of phenthoate in rice cooking is proposed to be 0.4.

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Utilization of Pruning Branch of Peach Tree as a Natural Dyeing Material (천연염료로서 복숭아나무 전정가지의 이용성)

  • Park Yun-Jum;Park Yong-Seo;Jang Hong-Gi;Heo Buk-Gu
    • Korean Journal of Plant Resources
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    • v.18 no.2
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    • pp.216-222
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    • 2005
  • This study was carried out to investigate the utilization of pruned branch of a peach tree as natural dyeing material. The dyestuff was extracted from pruned branch of a peach tree. we examined the dyeing ability on silk and cotten fabrics. The surface color of silk fabrics differed depending on mordants. E values in treatments of mordants ranged from 11.63 to 30.86 as compared to non-treatment. Dyeing properties of cotten fabrics were improved with a treatment of sulfate of iron as compared with of non-treatment. The values a increased when the slaked lime and sodium hydroxide were used as mordants, while the values b increased when copper sulfate, alum and apple vinegar were used. We investigated the surface colors of silk and cotten fabrics after washing three times. In general, the surface colors of silk and cotten fabrics between the treatments of all mordants and non-treatment were diminished by the washing. We also investigated the surface colors of silk and cotten fabrics when those were washed and treated by the light after soaking those into various pH solutions. The surface colors of silk and cotten fabrics were considerably preserved by the washing, the light and pH concentration. In conclusion, our results suggest that the dyestuffs from pruning branch of a peach tree would be useful as a natural dyeing material using the optimized conditions for silk and cotten dyeing.