• Journal of fish pathology
• /
• v.14 no.1
• /
• pp.11-15
• /
• 2001

For the production of healthy shrimp(Penaeus chinensis, Penaeus japonicus) seedlings free from the white spot baculovirus(WSBV), we tried to disinfect shrimp eggs with iodine. A relative hatching rate of over 50% has resulted from the treatments of fertilized eggs of fresh shrimps(P. chinensis) with an effective povidon-iodine concentration of 20-200 ppm for 30 seconds and 60 seconds, respectively. The 60 sec treatment group with a 20 ppm effective povidon-iodine concentration showed nearly a 50% relative hatching rate. The over 50 ppm treatment group, however, did not result in hatching show a relative hatching rate. These hatching rates were similar to those in the fertilized eggs of kuruma prawns treated with povidon-iodine solutions. In case of washing fertilized eggs with UV-irradiated seawater 33.3% relative hatching rate in fresh shrimps was much lower than 55.2% in kuruma shrimps. Treatment fertilized eggs of 2 species of shrimp with 200 ppm iodine for 30 sec did not affect the survival rate of the larvae until the early stage of the post larvae even though the hatching rates were low. In order to produce healthy shrimp seedlings non-infected by WSBV, the shrimp eggs should be washed with UV-irradated seawater and sterilized with a low povidon-iodine concentration for a short time.

• The Korean Journal of Physiology
• /
• v.23 no.1
• /
• pp.13-21
• /
• 1989

The present study was designed to investigate i) the action of various nucleotides on membrane permeability of rat red blood cell and hepatocyte for $Na^{+}$ and $Rb^{+}$ ii) the characteristics of purinoceptors on these cell membranes. Blood from Sprague-Dawley rats was obtained by carotid arterial cannulation. Red blood cells were then washed 3 times with saline at $4{\circ}C$. Hepatic parenchymal cells were isolated from rat livers by using a modification of the Berry and Friend (1969) method. For the $Na^{+}$ influx studies, isolated RBC and hepatocyte were incubated in incubation medium containing $^{22}Na^{+}0.2\;{\mu}Ci/ml$ at $37^{\circ}C$. After various time intervals samples were removed from the incubation flask and washed out 3 times with ice-cold washing solutions. Cells were destroyed by adding Triton X-100 and TCA solution. After centrifugation, the supernatants were assayed for $^{22}Na^{+}$ by gamma counter. $^{86}Rb^{+}$ was used to simulate $K^{+}$ in these $K^{+}efflux$ studies. Isolated hepatocytes were incubated for 60 min in the loading solution containing $^{86}Rb^{+}\;10\;{\mu}Ci/ml$ at $37^{\circ}C$. After loading, the cells washed out 3 times by centrifugation with washing solution. The cells were incubated in buffer solution at $37^{\circ}C$. At intervals thereafter, samples were removed and centrifuged. The supernatants were analyzed for $^{86}Rb^{+}$ by liquid scintillation counter. The main results of the experiments were: 1) ATP and ATPP increased in both $^{22}Na^{+}$ influx and $^{86}Rb^{+}$ efflux in the red blood cell. Although ADP showed a tendency to increase in RBC membrane permeability for $^{22}Na^{+}$ and $^{86}Rb^{+}$, the changes were not significantly different from the control. 2) The Significant changes in $^{22}Na^{+}$ and $^{86}Rb^{+}$ flux by ATP were also demonstrated in hepatocyte. ATPP and ADP showed a tendency to increase in hepatocyte membrane permeability for both ions. 3) Other nucleoside triphosphates-ITP, GTP and CTP-did not change in membrane permeability for $^{22}Na^{+}$ and $^{86}Rb^{+}$ in RBC and hepatocyte. In conclusion, not only ATP but also ATPP activate purinoceptors and change in membrane permeability for $Na^{+}$ and $K^{+}$. In order to activate purinoceptors on the cell membrane, the nucleotides have to possess intact adenine moiety and three phosphates or more in its molecule.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.40 no.4
• /
• pp.586-592
• /
• 2011

The storage quality of soybean sprouts washed with various electrolyzed waters was investigated. The washing solutions consisted of tap water (TW), sodium hypochlorite electrolyzed water (SHEW), and slightly acidic electrolyzed water (SAEW). The number of bacteria on the soybean sprouts after 5 min of exposure to TW, SHEW, and SAEW resulted in >0.5, 2.0, and 2.0 log CFU/g reductions, respectively. At both $4^{\circ}C$ and $25^{\circ}C$, the number of bacteria, weight loss ratio, and b value rapidly increased, and pH and L value rapidly decreased in soybean sprouts washed with TW. Whereas, the number of bacteria, pH, weight loss ratio, and color slowly changed in the soybean sprouts washed with SHEW and SAEW. Consequently, these results indicate that washing with electrolyzed water is an effective means of maintaining the quality and enhancing the shelf-life of soybean sprouts; both SHEW and SAEW reduced bacterial growth without affecting the other properties of soybean sprouts during storage.

• Food Science and Preservation
• /
• v.24 no.6
• /
• pp.827-833
• /
• 2017

This study was investigated the effects of various commercial sanitizers on microbial characteristics in fresh-cut iceberg lettuce during storage. For screening sanitizer, lettuce was cut and dipped in chlorine water ($0.2ml{\cdot}L^{-1}$), solution of organic acids such as ascorbic acid, citric acid, acetic acid, mixture of ascorbic acid and acetic acid (1-6%), and solutions of commercial sanitizers such as Formula 4$^{TM}$ (1,3,4%), Fresh produce wash$^{TM}$ (1,3,4%), Cleancol$^{TM}$ (1%), Chitochol$^{TM}$ (1%) and Natural Ca$^{TM}$ (0.1%) for 3 min, respectively. Washing lettuce with selected sanitizers resulted in reduction of aerobic bacteria of more than 2 log CFU/g. Initial pH of lettuce was related with the pH of sanitizers. pH ranged from 4.7 to 6.1 in Formula 4 (4%, pH 1.7) and Natural Ca (0.1%, pH 12.0), respectively. Chlorine water showed consistent and significant inhibition effect in all of microorganisms except total coliform. Over 3% of Formula 4 and Fresh produce wash were found to have high bactericidal activity among sanitizers. The sanitizers of chlorine water, Fresh produce wash, Chitochol and Natural Ca were effective in reducing yeast and mould populations. As coliform and E. coli, Formula 4 (4%) showed the highest bactericidal activity. The bactericidal effect of commercial sanitizers during storage varied with the kinds and concentrations of tested sanitizers. Although inhibition effect was not showed during storage, these results suggest that commercial sanitizers could be an alternative to chlorine for washing fresh-cut produce.

• Journal of Powder Materials
• /
• v.20 no.5
• /
• pp.359-365
• /
• 2013

In this study, we fabricated $Nd_2Fe_{14}B$ hard magnetic powders with various sizes via spray drying combined with reduction-diffusion process. Spray drying is widely used to produce nearly spherical particles that are relatively homogeneous. Thus, the precursor particles were prepared by spray drying using the aqueous solution containing Nd salts, Fe salts and boric acid with the target stoichiometric composition of $Nd_2Fe_{14}B$. The mean particle sizes of the spray-dried powders are in the range from one to seven micrometer, which are adjusted by controlling the concentrations of precursor solutions. After debinding the as-prepared precursor particles, ball milling was also conducted to control the particle sizes of Nd-Fe-B oxide powders. The resulting particles with different sizes were subjected to subsequent treatments including hydrogen reduction, Ca reduction and washing for CaO removal. The size effect of Nd-Fe-B oxide particles on the formation of $Nd_2Fe_{14}B$ phase and magnetic properties was investigated.

• Journal of Korean Society of Environmental Engineers
• /
• v.27 no.6
• /
• pp.606-613
• /
• 2005

The badge-type diffusive sampler for the measurement of formaldehyde in indoor air using three types of colorimetric methods such as chromotrophic acid(CTA), 3-methyl-2-benzothiazolinone hydrazone(MBTH), and 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole(AHMT) method. The washing of the collection filter with several cleaning solution was effected with satisfactory results, regardless of the types of cleaning solutions. The concentrations of absorbance solution in each colorimetric method were experimentally determined by considering the sampling rates. The variation blank values in each colorimetric method was below 15%. As compared with CTA and AHMT methods, the reproducibility of MBTH method was excellent and was below 10% relative standard deviation. The collected formaldehyde mass and time-weighted concentration had a good correlation (correlation coefficient > 0.93). The limit of detection and limit of quantitation, and minimum sampling time were closely correlated to the sampling rates for the measurement of formaldehyde in each method.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.112-112
• /
• 2018

산마늘은 마늘과 같이 독특한 향이 있어 입맛을 돋우며 웰빙식품으로 3월 중순에서 5월초에 채취하여 생채로 쌍을 먹거나 김치, 절임으로 생산되어 판매된다. 산마늘의 부가가치 향상을 위하여 살균소독 세척과 소포장용 필름포장방법을 구명하고자 본 실험을 수행하였다. 산마늘을 살균소독 세척수(수돗물, 0.2% 구연산 침지 후 50% 에탄올 분무처리)로 2분 세척하고 원심탈수 후, 포장필름은 $30{\mu}m$ oriented polypropylene (OPP), $30{\mu}m$ OPP 필름을 기본재료로 산소투과율 $5,000cc/m^2{\cdot}day{\cdot}atm$ (OPP_5)로 조정한 필름, $30{\mu}m$ micro perforated polypropylene (MPP) 필름에 밀봉하고, $30{\mu}m$ OPP필름에 개봉 포장하여 $4^{\circ}C$에서 42일 저장하였다. 산마늘은 구연산 침지 후 에탄올 분무세척 후 세척 전 보다 일반세균수가 1.5 log CFU/g 감소되었으며 수돗물 세척 후는 0.1 log CFU/g 정도 감소하여 수돗물 보다 구연산 침지 후 에탄올 분무에 의해 균수 감소효과가 보다 컸으며 저장기간이 경과한 후에도 그러한 경향은 유지되었다. 저장 35일 후 세척수에 관계없이 OPP 개봉과 MPP 밀봉 포장에서 의해 산마늘의 황화율과 부패율이 높게 나타났으며 OPP 밀봉에서 이취 발생 지수가 OPP_5 밀봉보다 높게 나타났다. 세척 및 포장처리에 의한 종합선도의 제한요인은 황화, 이취 및 부패취 발생 지수로 이를 종합하였을 때 산마늘의 상품성 향상을 위하여 소포장 하는 경우 구연산 침지 후 에탄올 분무 방법으로 세척한 다음 원심탈수하여 OPP_5필름으로 포장하는 것이 품질 향상에 적합하였으며 40일 이상 저장이 가능한 것으로 나타났다.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.10 no.1
• /
• pp.11-15
• /
• 1977

Brownish discoloration develops very rapidly in the storage of dried oyster. This undesirable browning is mainly caused by the series of reactions of sugar-amino condensation, enzymatic oxidation of tyrosine and/or the oxidative rancidity of lipids in the tissue of oyster. Sulfites are commonly used as inhibitors for Maillard type browning reactions in agricultural products. The inhibitory effect of sulfite treatment on canned oysters was also confirmed in some investigations. The results suggested that sulfites not only work on blocking tile amadori rearrangement but also on the reduction of free tyrosine which retards the progress of enzymatic oxidation of tyrosine tyrosinase. In this paper, the effect of sodium sulfite treatment on the reduction of reducing sugar and free tyrosine as a function i)f browning inhibition in oyster was tested and other treatment with glucose-oxidase and yeast were also applied. In preparation of samples, fresh oysters were soaked in sodium sulfite solution by various concentration for different treating times, washed in running water to remove the sulfite residue, and finally dried in the shade. In the result, the treatment of sodium sulfite was certainly effective on the reduction of both free tyrosine and reducing sugars in fresh oyster. The best results were obtained by the treatment of 0.5M sodium sulfite solution for 60 minutes each for soaking and washing. Treatment with, glucose-oxidase and yeast solutions, however, did appear somewhat effective but it required so much time for a certain effect that it seemed not practically applicable.

• Fashion & Textile Research Journal
• /
• v.11 no.5
• /
• pp.764-770
• /
• 2009

This study was conducted to analyze the wearing condition on gloves for pilots and to suggest the developments for pilot gloves design. This survey was performed with 99 pilots and the questionnaires included items about discomfort, materials and problems when wearing pilot gloves. The respondents felt uncomfortable on the finger tip, the hollow area of the hand and the end of thumb when they move their hands with the gloves. They replied that the finger length, palm length, and circumference of the fingers did not match well. Also they complained there was severe abrasion in the tip of the thumb, finger tips and the middle of the palm of the gloves. The result of these analyses supports the following suggestions. It is necessary to improve durability by applying the additional materials to the severely worn parts. Punching on the glove materials could improve sweat rate and ventilation, and careful washing should be accompanied to prevent the occurrence of linters and transformation after laundering. And it is needed to prepare an accurate and more diversified size system and to design the gloves fitted to the individual hands. With these in mind, it is required to propose solutions for pilot gloves with reference to the survey results in order to design appropriate pilot gloves in terms of movement, size and material. It was concluded that an analysis of the wearing condition and developments are useful for ergonomics pilot gloves design.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.10 no.3
• /
• pp.27-36
• /
• 1986

The purpose of this study was, to estimate the detergency of commercial detergent and to examine the interrelationship between detergency and detergent characteristics, detergent concentration, fiber characteristics, and Rolling-up phenomenon which is the major removal mechanism of oily soil. A mixture of oleic acid-olive oil was used as oily soil. The detergency was estimated by analysis of oleic acid on cotton ana polyester fabrics before and after washing by means of liquid scintillation counting. The Rolling-up of oily soil from PET film was observed and change of contact angle and removal time were measured. The results were as follows ; 1. In the case of soaps and powder synthetic detergents, the optimum concentrations we-re about $0.2\~0.3\%$. And detergencies of liquid synthetic detergents were considerably low, and the detergency was continually increased up with increasing concentration to $0.5\%$, which seemed to be caused by the lower pH than that of soap and powder synthetic deter-gent solutions. 2. As the effect of external or internal fiber structure; the detergency of cotton was lower than that of polyester. 3. In the observation of Rolling-up, the contact angle increased and the Rolling-up time became shorter with increasing detergent concentrations ($0.05\~0.5\%$). And it was confirmed that detergency was increased with Rolling-up effect. In addition, the study on the actual laundry condition was studied using the questionaire. From the results, about $76\%$ of households used the concentration of detergent roughly or excessively and housewives were highly concerned on the recommended dose, but grade of practice were very low.