• Title/Summary/Keyword: warm and cold stratification

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Effects of Temperature and Gibberellin Treatment on Embryo Development and Germination of Sambucus racemosa subsp. pendula Seeds (온도 및 지베렐린 처리가 말오줌나무 종자의 배발달 및 발아에 미치는 영향)

  • Kim, Hyun Jin;Lee, Ki Cheol;Suh, Gang-Uk
    • Journal of Korean Society of Forest Science
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    • v.102 no.2
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    • pp.204-209
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    • 2013
  • This study was conducted to determine types of seed dormancy in Sambucus racemosa subsp. pendula (Nakai) H.I. Lim & C.S. Chang, an endemic tree species of Korea, whose seeds have been considered difficult to germinate. Seeds of S. racemosa subsp. pendula were stratified at 25/15 or $5^{\circ}C$ for 0, 6, or 12 weeks (wks) and incubated at 15/6, 20/10, 25/15, or $30/20^{\circ}C$ (12/12 h) under 14 h photoperiod. To determine the effect of $GA_3$ on seed germination of S. racemosa subsp. pendula, seeds were treated with 0, 500, or 1000 ppm $GA_3$ and then germinated at 25/15 or $5^{\circ}C$. The change in embryo length was investigated at 25/15 or $5^{\circ}C$. Seeds given 12 wks of cold stratification germinated to 33.4% at $15/6^{\circ}C$ and to 25.4% for seeds given 6 wks of warm stratification + 12 wks of cold stratification at $20/10^{\circ}C$. At $25/15^{\circ}C$, seeds given 12 wks of warm stratification + 6 wks of cold stratification germinated to 26.0%, and to 28.2% for seeds given 12 wks of warm stratification + 12 wks of cold stratification at $30/20^{\circ}C$. Warm stratification alone did not germinate seeds throughout the experiment, regardless of the thermoperiod. Linear embryos began to grow after 60 days of incubation at 25/15 or $5^{\circ}C$. The embryo length at day 69 increased from 1.4 mm to 1.50 or 1.62 mm at 25/15 or $5^{\circ}C$, respectively. Embryos of S. racemosa subsp. pendula seeds grew better at $5^{\circ}C$ than at $25/15^{\circ}C$. Gibberellin was effective to break seed dormancy of S. racemosa subsp. pendula. Seeds treated with 500 ppm $GA_3$ germinated up to 40.0% at $25/15^{\circ}C$ and to 62.7% for those treated with 100 ppm $GA_3$ at $5^{\circ}C$. With these results, seeds of S. racemosa subsp. pendula have both nondeep complex and intermediate complex morphophysiological dormancy.

Effects of Gibberellin Treatment on the Dormancy-breaking and Germination Promotion of Carpinus laxiflora Seeds Stored for 16 Years (지베렐린 처리에 따른 서어나무 16년 저장 종자의 휴면타파 및 발아촉진 효과)

  • Han, Sim-Hee;Ku, Ja Jung;Kim, Du Hyun;Lim, Hyo-In
    • Journal of Korean Society of Forest Science
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    • v.108 no.4
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    • pp.531-539
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    • 2019
  • This study assessed the vitality of Carpinus laxiflora seeds stored for more than 15 years in order to discover optimal pre-treatment conditions for removingthe causes of seed dormancy and improving revitalization rate. Seeds were collected in October 2000 and stored at -18℃ for 16 years. Experiments to assess the revitalization ability of the seeds were performed under the following conditions: a controlled environment; in warm stratification (30 days at 23℃); in cold stratification (30 days at 4℃ andfor 30, 45, 60, and 120 days); gibberellin (GA3) treatment (24 hours per day in GA3 solutions of 100, 500, 1000, 2000, and 3000 mg·L-1); and both cold stratification and GA3 treatment (30 days at 4℃, then in a GA3 solution of 100, 500, and 1000 mg·L-1 for 2 hours). The average germination percentage(GP) of untreated seeds was 2%, and the average GP of warm-stratification seeds was 10%. Cold-stratification seeds had the highest GP at 81% for the 45-day process, while the 120-day cold-stratification seeds had the lowest GP at 67.3%. The average GP of seeds treated with GA3 ranged from 77% (100 mg·L-1) to 99% (1000 mg·L-1), indicatingsignificant differences between the treatment concentrations. The treatment effect of GA3 was highest at 500 mg·L-1 and 1000 mg·L-1, and lowest at 100 mg·L-1. The average GP of seeds treated with GA3 following cold stratification was 68%, which was lower than the cold stratification-only (73.2%) and GA3-only (88.4%) treatments. A comprehensive comparison of the seed germination characteristics according to the four treatments determined that a GA3 500 mg·L-1 pre-treatment, with the highest average GP, was ideally suited to the revitalization of long-term stored C. laxiflora seeds. Consequently, C. laxiflora stored at -18℃ for 16 years indicated strong vitality and could be regenerated by proper pre-treatment.

Effects of Water Temperature Inversion on the Stratification Variation in October and December in the South Sea of Korea (한국 남해에서 10월과 12월의 수온역전현상이 성층변동에 미치는 영향)

  • Lee, Chung-Il;Koo, Do-Hyung
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.15 no.3
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    • pp.165-171
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    • 2009
  • In order to illustrate the effects of water temperature inversion on the stratification variation in the South Sea of Korea, water temperature, salinity, and density measured in October and December 1999 by National Fisheries Research and Development Institute were reviewed. In October and December of 1999, temperature inversion occurred mainly between 25m and 75m, and in particular in depth of water, in December temperature inversion layer also was formed in the surface layer. In case of October and December, the Tsushima Warm Current (TWC), warm and saline water, was one of motors, and in December, influence of surface cold water was added Although northerly wind prevails in October and December, in October, expanding of the South Korean Coastal Waters (SKCW) towards offshore is not clear, but in December when wind speed is relatively greater than that in October and strength of the TWC become weak, the SKCW spreads towards offshore through the upper layer. Stratification variation was higher along the area where temperature inversion occurred.

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Seed Dormancy Type and Germination Characteristics in Tiarella polyphylla D. Don Native to Korea (한반도 자생식물 헐떡이풀 종자의 휴면유형과 발아특성)

  • Choi, Han;Lee, Seung Youn;Rhie, Yong Ha;Lee, Jeong Ho;Kim, Sang Yong;Lee, Ki Cheol
    • Korean Journal of Plant Resources
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    • v.31 no.4
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    • pp.363-371
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    • 2018
  • Tiarella polyphylla D. Don is a native plant distributed only in Ulleung Island in Korea and has been traditionally used for medicinal purposes, although it is also used ornamentally. This study was conducted to determine the requirements for dormancy break and germination and to classify the type of seed dormancy. The experiments were performed with cold stratification (0 or 12 weeks at $5^{\circ}C$), warm stratification (0, 4, 8, or 12 weeks at $23^{\circ}C$, followed by 8 weeks at $5^{\circ}C$, and then incubation at $23^{\circ}C$), and $GA_3$ treatments (0, 10, 100, or 1000 mg/L). The treated seeds were incubated on aseptic media at room chamber ($23^{\circ}C$, a 16h photoperiod of fluorescent lamps with $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). The seeds were dispersed in nature as underdeveloped embryos with no physical barrier to absorb water to prevent water absorption. However, the seeds did not germinate for 30 days after sowing without any pre-treatments. Thus, the seeds had morphological dormancy (MD) and physiological dormancy (PD). The final germination percentage following cold stratification (0 or 12 weeks) was 66.7% and 45.6%, respectively. The cold stratification delayed seed germination by about 3 weeks. In the warm stratification experiment (0, 4, 8, or 12 weeks), the final germination percentage was 21.1%, 27.8%, 41.1%, and 57.8%, respectively, 20 weeks after sowing. The embryos of the T. polyphylla seed grew in relatively warm temperatures ($23^{\circ}C$). $GA_3$ application overcame seed dormancy and promoted germination. Following $GA_3$ treatment (0, 10, 100, or 1000 mg/L), the final germination percentage was 33.3%, 45.0%, 42.5%, and 72.5%, respectively. These results suggest that the T. polyphylla seeds had non-deep simple morphophysiological dormancy (MPD) and $GA_3$ treatment could be used as a substitute for warm stratification for breaking seed dormancy. To our knowledge, this is the first report of seed dormancy characteristics of the genus Tiarella native to Korea.

Dormancy-breaking and Germination of Chelidonium majus L. subsp. asiaticum H. Hara Seeds by Stratification and Gibberellins

  • Boran Ji;Hayan Lee;Kyungtae Park;Sang Yeob Lee;Bo-Kook Jang;In Hwan Chae;Chung Youl Park;Sung Pil Kwon;Deug-Chan Lee;Ju-Sung Cho
    • Korean Journal of Plant Resources
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    • v.35 no.6
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    • pp.796-804
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    • 2022
  • The demand for Chelidonium majus L. subsp. asiaticum H. Hara is expected to increase due to its pharmacological properties such as antibacterial, antioxidant, and anti-inflammatory effects. However, an effective propagation system for this species has not yet been established. This study was conducted to analyze the seed dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara native to Korea and establish a mass propagation system. The dormancy type was primarily classified by analyzing the general information of the collected seeds. The seed dormancy breaking was investigated by comparing the effects of cold stratification (0, 2, 4, 8, 10, or 12 weeks) with warm stratification (S, summer temperature, 25/15℃) and intermediate temperature stratification (A, autumn temperature, 15/10℃) of alternating temperature stratification (S12-A4 or S12-A8 weeks). After dormancy break, 500 mg/L GA3 and GA4+7 treatment replaced cold stratification and improved seed germination. The results of this study are expected to provide basic data for future seed propagation and mass propagation by analyzing the dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara seeds.

The Yellow Sea Warm Current and the Yellow Sea Cold Bottom Water, Their Impact on the Distribution of Zooplankton in the Southern Yellow Sea

  • Wang, Rong;Zuo, Tao
    • Journal of the korean society of oceanography
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    • v.39 no.1
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    • pp.1-13
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    • 2004
  • The Yellow Sea Warm Current (YSWC) and the Yellow Sea Cold Bottom Water (YSCBW) are two protruding features, which have strong influence on the community structure and distribution of zooplankton in the Yellow Sea. Both of them are seasonal phenomena. In winter, strong north wind drives southward flow at the surface along both Chinese and Korean coasts, which is compensated by a northward flow along the Yellow Sea Trough. That is the YSWC. It advects warmer and saltier water from the East China Sea into the southern Yellow Sea and changes the zooplankton community structure greatly in winter. During a cruise after onset of the winter monsoon in November 2001 in the southern Yellow Sea, 71 zooplankton species were identified, among which 39 species were tropical, accounting for 54.9 %, much more than those found in summer. Many of them were typical for Kuroshio water, e.g. Eucalanus subtenuis, Rhincalanus cornutus, Pareuchaeta russelli, Lucicutia flavicornis, and Euphausia diomedeae etc. 26 species were warm-temperate accounting for 36.6% and 6 temperate 8.5%. The distribution pattern of the warm water species clearly showed the impact of the YSWC and demonstrated that the intrusion of warmer and saltier water happened beneath the surface northwards along the Yellow Sea Trough. The YSCBW is a bottom pool of the remnant Yellow Sea Winter Water resulting from summer stratification and occupy most of the deep area of the Yellow Sea. The temperature of YSCBW temperature remains ${\leq}{\;}10^{\circ}C$ in mid-summer. It is served as an oversummering site for many temperate species, like Calanus sinicus and Euphaisia pacifica. Calanus sinicus is a dominant copepod in the Yellow Sea and East China Sea and can be found throughout the year with the year maximum in May to June. In summer it disappears in the coastal area and in the upper layer of central area due to the high temperature and shrinks its distribution into YSCBW.

A Study on the Propagation of Lindera erythroearpa Makino (비목나무 번식에 관한 연구)

  • 이동철;심경구;서병기
    • Asian Journal of Turfgrass Science
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    • v.9 no.1
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    • pp.1-10
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    • 1995
  • This study was carried out to investigate the method of propagation of Lindera erythrocarpa Makino. The results were as follows: 1. After the treatment of warm stratification for 1 month followed by cold stratification for 3 months, the germination ratio of seeds of Lindera erythrocarpa was from 84.6 to 93.6 percent. But, dry stored seeds lose their viability Quickly and should be placed in pretreatment im-mediately. Also, the seeds of control district germinated from 68.6 to 74.6 percent. So it was confirmed that the seed of Lindera erythocaeicarpa was not doubly dormant. 2. Softwood cuttings of Lindera erythrocarpa with 5,000 ppm IBA on August 4. 1992 resulted in the best rooting of 95.0 percentage. 3.Twenty percent of the graft union were obtained in the green wood grafting in Lindera erythrocarpa on June 7, 1993. And five percent of the graft union were obtained in the hard wood grafting in Lindera erythrcoar pa on April 6. 1993.

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Coastal Stratification Induced by Oceanographic Conditions of Open Sea in the East Sea on February, 2013 (2013년 2월 동해의 근해 해황에 의한 연안 성층)

  • Choi, Yong-Kyu;Kim, Sang-Woo;Jeong, Hee-Dong;Shim, Jeong-Min;Kwon, Kee-Young
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.19 no.4
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    • pp.327-333
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    • 2013
  • In order to see the stratification phenomenon in the coastal area induced by oceanographic conditions of the open sea, we analyzed the CTD (Conductivity-Temperature-Depth) data taken from the oceanographic survey on February 16~28, 2013. The stratification in Jukbyun coast was stronger than those of Sokcho and Gampo coast. Jukbyun line (104 line in the Serial Oceanographic Observation of National Fisheries Research and Development Institute) showed the anticyclonic eddy in the vertical distribution of temperature. The isotherm of $10^{\circ}C$ was concaved to the depth of 200 m in the middle station (station no. 9) of the line 104. It showed above $4^{\circ}C$ in positive temperature anomaly in the depth of 100~200 m in the middle station (station no. 9) of the line 104. This positive temperature anomaly was stretched to the coastal area with shallower depth. It is suggested that the stratification in Jukbyun coast was resulted from the onshoring of the Ulleung warm eddy. The movement of warm eddy may be act as a block to migration of cold water fishes like cod.

A Study on the Seed Germination and Seedling Density of Fraxinus mandshruica in Nursery (포지(圃地)에 있어서 들메나무의 종자발아(種字發芽) 및 묘목생육밀도(苗木生育密度)에 관한 연구(研究))

  • Chung, Seung Ho;Kim, Jong Won;Lee, Geun Soo
    • Journal of Korean Society of Forest Science
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    • v.63 no.1
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    • pp.9-11
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    • 1984
  • This study was carried out to compare the seed germination and seedling growth of Fraxinus mandshurica growing in natural forests of the interior of Korea. Three stimulation methods were used for germination and the seedlings were planted with various densities. The results obtained were summarized as follows: 1) It was suggested that the 80 seedlings per square meter were proper densities for their growth at a nursery bed. 2) Among the three stimulation methods, 19.9% of germination was shown by the moisture storage, 2.3% by cold and warm stratification, and 0.4% by cold stratification. 3) The 8.1% of the total seedlings showed secondary height growth.

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Seed propagation and softwood cutting of native styrax japonicus for landscape tree uses (한국 자생 때죽나무의 조경수 이용을 위한 번식방법에 관한 연구)

  • 권오준;심경구;하유미
    • Journal of the Korean Institute of Landscape Architecture
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    • v.25 no.2
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    • pp.9-19
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    • 1997
  • These studies were carried out to investigate seed propagation method and conducted using stem cuttings of Styrax japonicus for using as a woody landscape plant. The results are obtained as follows. After the H\sub2\SO\sub 4\ and GA\sub 3\ treatment to the seeds of Styrax japonicus, three months of warm treatment followed by cold stratification for four months increased seed germination rate. The rooting rate of Styrax japonicus was increased by the treatment with concentrated IBA, especially highest at 7,000ppm. Rooting percentages were highest for cuttings taken on July 15, 1995. Therefore, the most effective method for rooting of Styrax japonicus was treatment with 7,000ppm IBA on July 15 cuttings, which showed rooting rate of over 90%. Half-leaf-remained cuttings were more effective than those with two leaves on rooting of Styrax japonicus. Dipping for 10 seconds in IBA solution resulted in rooting rate of over 70%. The longer the treatment time the worse the rooting.

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