• Title/Summary/Keyword: w3/w6 ratio

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Development of an Onion Vinegar Beverage Containing Yuza (Citrus junos Sieb ex Tanaka) and Its Biological Activity (유자 첨가 양파발효음료의 제조 및 생리활성)

  • Jeong, Eun-Jeong;Cha, Yong-Jun
    • Journal of Life Science
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    • v.26 no.5
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    • pp.563-570
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    • 2016
  • Onion vinegar has an undesirable flavor and taste that results from alcohol and acetic acid production from fermentation. In this study, we have used onion vinegar to develop an onion vinegar beverage with better sensory quality. The objective of this study was to determine the optimum blending ratio by using response surface methods to produce an onion vinegar beverage containing Yuza (Citrus junos Sieb ex Tanaka). The optimal formula for a fermented onion beverage was determined using a central composite design by the response surface methodology. The independent variables were obtained by regression analysis of the reaction surface of brown sugar, apple extracts and Yuza extracts. The optimum mixing ratio for onion vinegar:water:brown sugar:apple extracts:Yuza extracts was 6.0:77.6:4.9:9.2:2.3 (w/w). The actual overall acceptance was 7.08 under optimum conditions, which was close to the maximum predicted value of 6.96. The concentration of phenolic compounds, total flavonoids, and quercetin present in the onion vinegar beverage was 14.8 mg/100 g, 2.6 mg/100 g and 1.4 mg/100 g, respectively. The onion vinegar beverage showed antimicrobial activity against Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Enterobacter aerogenes. It also showed antioxidant effects, with a DPPH radical inhibition rate of 18.2% and superoxide dismutase (SOD)-like activity of 11.5%. In conclusion, the onion vinegar beverage described here seems to have nutritional value and potential biological activity.

Studies on Lipids in Fresh-Water Fishes 6. Comparison of Lipid Components between Wild and Cultured Snakehead, Channa argus (담수어의 지질에 관한 연구 6. 천연 및 양식 가물치의 지질성분 비교)

  • CHOI Jin-Ho;BAE Tae-Jin;BYUN Dae-Seo;YOON Tai-Heon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.18 no.4
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    • pp.309-315
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    • 1985
  • This study was designed to compare the lipid components between wild and cultured snakehead, Channa argus. The lipid components of cultured snakehead were analyzed and compared with that of wild snakehead. In both edible portion and viscera, the lipid content in cultured, snakehead was higher than that in wild one. In the fatty acid composition of neutral lipid in edible portion, percentages of $C_{14:0},\;C_{16:0},\;C_{18:1},\;C_{20:5},\;C_{22:5}\;and\;C_{22:6}$ in cultured snakehead were higher than those in wild one, while percentages of $C_{18:0},\;C_{16:1},\;C_{18:2},\;C_{18:3},\;C_{20:4}\;and\;C_{22:4}$ lower. In the case of phospholipid in edible portion, percentages of $C_{16:0},\;C_{18:1}\;and\;C_{22:6}$ in cultured snakehead were higher than those in wild one, while percentages of $C_{16:1},\;C_{18:2},\;C_{18:3},\;C_{20:4},\;C_{22:4}\;and\;C_{22:5}$ lower. The unsaturation (TUFA/TSFA) and w3 highly unsaturated fatty acid content (w3 HUFA) of neutral lipid in cultured snakehead were higher than those in wild one, while those of phospholipid lower. The essential fatty acid contents (TEFA) of both neutral and phospholipids in wild snakehead were higher than those in cultured one. In the ratio (A/B) of fatty- acid content (A) in cultured snakehead to that (B) in diet, the A/B ratios of $C_{18:2\;w6},\;C_{18:3\;w3},\;C_{20:5\;w3}\;and\;C_{22:6\;w3}$ were 0.3 to 2.5 times, and it is considered to be related to the biosynthesis of polyenoic acids and growth rate of cultured snakehead.

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Targeted Gene Delivery of the Cationic Lipid Emulsion System Containing Folate-PEG-DPPE (Folate-PEG-DPPE를 포함하는 양이온성 지질 에멀젼 시스템의 표적화 유전자 전달)

  • Kwon, Sang-Kyoo;Jeong, Kwan-Ho;Kim, Young-Jin
    • Polymer(Korea)
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    • v.33 no.3
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    • pp.213-218
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    • 2009
  • A cationic lipid emulsion containing 1,2-dioleoyl-3-trimethylammonium-propane(DOTAP), Tween80, squalene has been prepared as a gene delivery system. In order to increase the transfection efficiency of gene carrier, folate was used as the tumor-targeting ligand that was attached on PEG-DPPE. HeLa and 293 cells were used for the in vitro transfection experiment. HeLa cell is a folate-positive cell line. The mean particle sizes of polymeric lipid system and DNA/lipid complex system were 206.6 nm and 150.5 nm, respectively. The transfection efficiencies of our carriers(4:l(w:w) complex ratio)were 100 times higher than that of DOTAP only emulsion due to the targeting effect of folate.

Enzymatic Synthesis of Fructose-based Sugar Fatty Acid Ester Using Methyl Fructoside (메틸프룩토시드를 이용한 과당계열 당 지방산 에스테르의 효소적 합성)

  • 허주형;김해성
    • KSBB Journal
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    • v.13 no.6
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    • pp.706-717
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    • 1998
  • Enzymatic synthesis of fructose-based sugar fatty acid esters, such as methyl fructoside oleic acid mono and diester, was investigated using methyl fructoside as a sugar starting material. For the production of methyl fructoside fatty acid monoester by solvent process, 2-methyl 2-propanol was found to be a god reaction medium resulting a higher yield and productivity due to its high sugar solubility. The yield and productivity of methyl fructoside oleic acid monoester were 70% and 12.6g/L-hr, respectively, when molar ratio of methyl fructoside, initial concentration of methyl fructoside, enzyme(Novozym 435) content, and reaction temperature were 3:1, 200g/L, 1%(w/v), and $60^{\circ}C$, respectively. Methyl fructoside oleic acid diester was prepared by lipase-catalyzed diacylation of methyl fructoside and oleic acid in the solvent-free process. Maximum yield of 98% and productivity of 140g/L-hr were achieved when molar ratio(methyl fructoside and oleic acid) of 1:2 enzyme content of 10%(w/v) and reaction temperature of $70^{\circ}C$ were applied for the operating conditions under a reduced pressure of 20∼200 mmHg.

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On the Generalized Empirical Equation for Effective Wellbore Radius (유효우물반경 산정 경험공식의 일반화에 대하여)

  • Choi, Byong-Soo
    • Journal of Soil and Groundwater Environment
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    • v.13 no.3
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    • pp.15-20
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    • 2008
  • The investigation on the previous paper(Jn. of KoSSGE vol.12, no.3), which proposed three empirical equations for the different aquifers was carried out. To draw out a single equation from the three different equations the mean value of the involution factors was adopted allowing different proportional coefficients for the different aquifers. On the other hand it was found that the square root value of storativity ratio, ${\sqrt{{S_p}/{S_o}}}$ in each well is almost equal to its wellbore radius ratio, ${r_e}/{r_w}$. From this fact, the proportional coefficients can be substituted with assumed aquifer storativity and a generalized empirical equation for the effective wellbore radius has been derived.

Extraction of La(III) by a nonionic microemulsion containing D2EHPA in hollow fiber contactor

  • Ou, Huilin;Gong, Fuzhong;Tang, Yanxia;Luo, Yan;Liu, Liheng
    • Membrane and Water Treatment
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    • v.12 no.2
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    • pp.75-82
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    • 2021
  • This study aimed to prepare a W/O nonionic microemulsion system(MEs) consisting of OP-4[polyoxyethylene(4) nonylphenol], OP-7[polyoxyethylene(7) nonylphenol], 1-hexanol, D2EHPA, kerosene and HCl solution and applied to the extraction of La(III) from chloride aqueous solution within the polysulfone hollow fiber contactor (HFC),laboratory-scale experiments were carried out to investigate the recovery of La(III) using as-prepared microemulsion from the simulation wastewater containing La(III),Al(III) and Fe(III). The right weight ratio(Rs) of OP-4 to OP-7 was firstly confirmed through determination of the solubilization capacity of HCl solution(W0,HCl) in microemulsion, the effect of several factors such as the HCl concentration, temperature and effective extraction time on the extraction efficiency of La(III) was discussed. Results showed that the acceptable Rs was 4:6 to prepare the W/O MEs. The extraction yield of La(III) increased with the increasing of HCl concentration, temperature and effective extraction time and reaches to 97.3% while using five-stage modules. The recovery yield of La(III) from simulation La-bearing wastewater was 90.6%.

Microwave Mediated Production of FAME from Waste Cooking Oil : Optimization of Process Parameters by RSM (마이크로웨이브를 이용한 폐식용유로부터 FAME의 제조 : RSM에 의한 공정변수 최적화)

  • Hong, In Kwon
    • Applied Chemistry for Engineering
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    • v.31 no.2
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    • pp.172-178
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    • 2020
  • In this study, the optimization of the biodiesel production process from waste oil using microwave with response surface methodology (RSM) was conducted. The microwave irradiation time and power in addition to the alcohol/oil mole ratio were chosen as process parameters. Also the fatty acid methyl ester (FAME) content (over 96.5%) and kinematic viscosity (1.9~5.5 cSt) were selected as response values. From basic experiments, the range of quantitative factors were set as following; 4~6 min, 400~600 W, and 7~9 for the microwave irradiation time and power, and alcohol/oil molar ratio, respectively. The optimum conditions for the methanolysis were 5.0~5.1 min, 481.3~525.5 W, 7.9~8.4, and 2.0 or 3.0 mg KOH/g for the microwave irradiation time and power, methanol/oil molar ratio, and each acid value, respectively. The FAME content and kinematic viscosity were predicted as 97.49~96.34% and 4.01~4.12 cSt, respectively, under the condition above. Under the optimum experimental conditions, the results showed that the FAME content and kinematic viscosity of 97.82~96.42% and 4.07~4.16 cSt, respectively were measured and the mean error rates were 0.22% and 0.98%, respectively.

Characterization of a Novel Lipopolysaccharide Biosurfactant from Klebsiella oxitoca

  • Kim, Pil;Kim, Jung-Hoe
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.6
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    • pp.494-499
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    • 2005
  • The chemical, physical, and emulsifying properties of BSF-1, which is an extracellular lipopolysaccharide biosurfactant produced by Klebsiella oxytoca strain BSF-1, were studied. BSF-1 was found to be composed mainly of carbohydrate and fatty acids. The average molecular weight was $1,700{\sim}2,000 kDa$. The polysaccharide fraction contained L-rhamnose, D-galactose, D-glucose, and D-glucuronic acid at a molar ratio of 3:1: 1:1. The fatty acid content was 1.1 % (w/w) and consisted mainly of palmitic acid (C16:0), 3-hydroxylauric acid (3-OH-C12:0), and lauric acid (C12:0). In terms of thermal properties, BSF-1 was revealed to have inter- and intra-molecular hydrogen bonds. The hydrodynamic volume (intrinsic viscosity) of BSF-1 was 22.8dL/g. BSF-1 could be maintained as a stable emulsion for 48 h through a low-level reduction in surface tension. The optimal emulsification temperature was $30^{\circ}C$. Emulsification by BSF-1 was efficient at both acidic and neutral pH values.

A low-power multiplying D/A converter design for 10-bit CMOS algorithmic A/D converters (10비트 CMOS algorithmic A/D 변환기를 위한 저전력 MDAC 회로설계)

  • 이제엽;이승훈
    • Journal of the Korean Institute of Telematics and Electronics C
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    • v.34C no.12
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    • pp.20-27
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    • 1997
  • In this paper, a multiplying digital-to-analog converter (MDAC) circuit for low-power high-resolution CMOS algorithmic A/D converters (ADC's) is proposed. The proposed MDAC is designed to operte properly at a supply at a supply voltge between 3 V and 5 V and employs an analog0domain power reduction technique based on a bias switching circuit so that the total power consumption can be optimized. As metal-to-metal capacitors are implemented as frequency compensation capacitors, opamps' performance can be varied by imperfect process control. The MDAC minimizes the effects by the circuit performance variations with on-chip tuning circuits. The proposed low-power MDAC is implementd as a sub-block of a 10-bit 200kHz algorithmic ADC using a 0.6 um single-poly double-metal n-well CMOS technology. With the power-reduction technique enabled, the power consumption of the experimental ADC is reduced from 11mW to 7mW at a 3.3V supply voltage and the power reduction ratio of 36% is achieved.

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Isolation of Chitin from Crab Shell Waste (게 가공 폐기물로부터 키틴의 분리)

  • 노홍균;이문이
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.1
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    • pp.105-113
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    • 1995
  • Procedures for isolatin of chitin have been developed from crab(Chionoecetes opilio) shell waste with 26. 65% chitin on a dry basis. Optimal conditions for demineralization of crab shell were 1N HCl at ambient temperature for 30min with a solids to solbent ratio of 1 : 15(w/v). Optimal deproteinization involved treatment with 5% NaOH at $65^{\circ}C$ for 1 hr with a solids to solvent ratio of 1 : 15(w/v). Effective decoloration was achieved by bleaching with 0.32% sodium hypochlorite solution for 3min with a solids to solvent ratio of 1 : 10(w/v). Particular attentin was given to characterization of the physicochemical properties of the crab chitin. Chitins, from four different mesh sizes of crab shell, did not show significant differences in nitrogen and ash compositions. Bleaching decreased the viscosity of chitin but did not affect its solubility.

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