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Targeted Gene Delivery of the Cationic Lipid Emulsion System Containing Folate-PEG-DPPE  

Kwon, Sang-Kyoo (Department of Applied Chemistry and Biological Engineering, Chungnam National University)
Jeong, Kwan-Ho (Department of Applied Chemistry and Biological Engineering, Chungnam National University)
Kim, Young-Jin (Department of Applied Chemistry and Biological Engineering, Chungnam National University)
Publication Information
Polymer(Korea) / v.33, no.3, 2009 , pp. 213-218 More about this Journal
Abstract
A cationic lipid emulsion containing 1,2-dioleoyl-3-trimethylammonium-propane(DOTAP), Tween80, squalene has been prepared as a gene delivery system. In order to increase the transfection efficiency of gene carrier, folate was used as the tumor-targeting ligand that was attached on PEG-DPPE. HeLa and 293 cells were used for the in vitro transfection experiment. HeLa cell is a folate-positive cell line. The mean particle sizes of polymeric lipid system and DNA/lipid complex system were 206.6 nm and 150.5 nm, respectively. The transfection efficiencies of our carriers(4:l(w:w) complex ratio)were 100 times higher than that of DOTAP only emulsion due to the targeting effect of folate.
Keywords
cationic lipid emulsion; folate; PEG; DOTAP; targeted gene delivery;
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