Browse > Article

Characterization of a Novel Lipopolysaccharide Biosurfactant from Klebsiella oxitoca  

Kim, Pil (Biotechnology Major, The Catholic University of Korea)
Kim, Jung-Hoe (Department of Biological Sciences, KAIST)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.10, no.6, 2005 , pp. 494-499 More about this Journal
Abstract
The chemical, physical, and emulsifying properties of BSF-1, which is an extracellular lipopolysaccharide biosurfactant produced by Klebsiella oxytoca strain BSF-1, were studied. BSF-1 was found to be composed mainly of carbohydrate and fatty acids. The average molecular weight was $1,700{\sim}2,000 kDa$. The polysaccharide fraction contained L-rhamnose, D-galactose, D-glucose, and D-glucuronic acid at a molar ratio of 3:1: 1:1. The fatty acid content was 1.1 % (w/w) and consisted mainly of palmitic acid (C16:0), 3-hydroxylauric acid (3-OH-C12:0), and lauric acid (C12:0). In terms of thermal properties, BSF-1 was revealed to have inter- and intra-molecular hydrogen bonds. The hydrodynamic volume (intrinsic viscosity) of BSF-1 was 22.8dL/g. BSF-1 could be maintained as a stable emulsion for 48 h through a low-level reduction in surface tension. The optimal emulsification temperature was $30^{\circ}C$. Emulsification by BSF-1 was efficient at both acidic and neutral pH values.
Keywords
biosurfactant; lipopolysaccharide; isolation; characterization; Klebsiella oxytoca;
Citations & Related Records

Times Cited By Web Of Science : 6  (Related Records In Web of Science)
Times Cited By SCOPUS : 6
연도 인용수 순위
1 Desai, J. D. and I. M. Banat (1997) Microbial production of surfactants and their commercial potential. Microbiol. Mol. Biol. Rev. 61: 47-64
2 Garcia-Junco, M., C. Gomez-Lahoz, J. L. Niqui-Arroyo, and J. J. Ortega-Calvo (2003) Biosurfactant- and biodegradation- enhanced partitioning of polycyclic aromatic hydrocarbons from nonaqueous-phase liquids. Environ. Sci. Technol. 37: 2988-2996   DOI   ScienceOn
3 Lin, F. M. and Y. Pomeranz (1968) Effect of borate on colorimetric determinations of carbohydrates by the phenol- sulfuric acid method. Anal. Biochem. 24: 128-131   DOI   ScienceOn
4 Lu, X. X., X. Zhang, G. H. Li, and W. H. Zhang (2003) Production of biosurfactant and its role in the biodegradation of oil hydrocarbons. J. Environ. Sci. Health. A Tox. Hazard Subst. Environ. Eng. 38: 483-492   DOI   ScienceOn
5 Fiechter, A. (1992) Biosurfactants: moving towards industrial application. Trends Biotechnol. 10: 208-217   DOI   PUBMED   ScienceOn
6 Menezes Bento, F., F. A. de Oliveira Camargo, B. C. Okeke, and W. T. Frankenberger, Jr. (2005) Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiol. Res. 160: 249-255   DOI   ScienceOn
7 Kim, J. H., W. H. Jeong, T. B. Karegoudar, and C. K. Kim (2002) Stable degradation of benzoate by Klebsiella oxytoca C302 immobilized in alginate and polyurethane. Biotechnol. Bioprocess Eng. 7: 347-351   DOI   ScienceOn
8 Miller, R. M. (1995) Biosurfactant-facilitated remediation of metal-contaminated soils. Environ. Health Perspect. 103 Suppl 1: 59-62
9 Kim, P., D. K. Oh, J. K. Lee, S. Y. Kim, and J. H. Kim (2000) Biological modification of the fatty acid group in an emulsan by supplementing fatty acids under conditions inhibiting fatty acid biosynthesis. J. Biosci. Bioeng. 90: 308-312   DOI
10 Kim, P., S. W. Kim, S. Y. Kim, and J. H. Kim (1998) Effects of hydrodynamic volume of anionic lipopolysaccharide, emulsan, on emulsifying activity. Biosci. Biotechnol. Biochem. 62: 603-604   DOI   ScienceOn
11 Kim, P. (1998) Structure-Emulsifying Activity Relationship of a Biosurfactant Emulsan and Characterization of a Novel Lipopolysaccharide Biosurfactant. in Ph.D. thesis. KAIST: Daejeon. Korea
12 Kim, Y. J., T. W. Kim, H. Chung, C. I. Kwon, and S. Y. Jeong (2001) Counterion effects on transfection activity of cationic lipid emulsion. Biotechnol. Bioprocess Eng. 6: 279-283   DOI   ScienceOn
13 Gutnick, D. L., E. Rosenberg, I. Belsky, and Z. Zinaida (1983) alpha-Emulsan. US Patent 4,395,354
14 Kim, P., D. K. Oh, S. Y. Kim, and J. H. Kim (1997) Relationship between emulsifying activity and carbohydrate backbone structure of emulsan from Acinetobacter calcoaceticus RAG-1. Biotechnol. Lett. 19: 457-459   DOI   ScienceOn
15 Zuckerberg, A., A. Diver, Z. Peeri, D. L. Gutnick, and E. Rosenberg (1979) Emulsifier of Arthrobacter RAG-1: chemical and physical properties. Appl. Environ. Microbiol. 37: 414-420
16 Wei, Q. F., R. R. Mather, and A. F. Fotheringham (2005) Oil removal from used sorbents using a biosurfactant. Bioresour. Technol. 96: 331-334   DOI   ScienceOn
17 Belsky, I., D. L. Gutnick, and E. Rosenberg (1979) Emulsifier of Arthrobacter RAG-1: determination of emulsifierbound fatty acids. FEBS Lett. 101: 175-178   DOI   ScienceOn
18 Choi, D. B., K. A. Cho, W. S. Cha, and S. R. Rhy (2004) Effect of Triton X-100 on compactin production from Penicillium citrinum. Biotechnol. Bioprocess Eng. 9: 171- 178   DOI   ScienceOn
19 Li, J., S. Y. B. Hu, and J. M. Wiencek (2001) Development of a supported emulsion liquid membrane system for propionic acid separation in a microgravity environment. Biotechnol. Bioprocess Eng. 6: 426-432   DOI   ScienceOn
20 Stellner, K., H. Saito, and S. I. Hakomori (1973) Determination of aminosugar linkages in glycolipids by methylation. Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen. Arch. Biochem. Biophys. 155: 464-472   DOI   ScienceOn