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Proteome characterization reveals the role of pollen and pistil of W22 (ga1; Ga1) in maize

  • Roy, Swapan Kumar;Yu, Jin;Kamal, Abu Hena Mostafa;Kwon, Soo Jeong;Cho, Kun;Cho, Seong-Woo;So, Yoon-Sup;Woo, Sun Hee
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.129-129
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    • 2017
  • The first key point to the successful pollination and fertilization in plants is the pollen pistil interaction, referring to the cellular and molecular levels, which mainly play active roles in limiting gene flow among maize populations and between maize and teosinte. This study was carried out to identify proteins and investigate the mechanism of gametophytic factors using protein analysis. W22 (ga1); which didn't carry a gametophytic factor and W22 (Ga1), a near iso-genic line were used for the proteome investigation. SDS-PAGE was executed to investigate proteins in the pollen and pistil of W22 (ga1) and W22 (Ga1). A total of 44 differentially expressed proteins were identified in the pollen and pistil on SDS-PAGE using LTQ-FTICR MS. Among the 44 proteins, a total of 24 proteins were identified in the pollen of W22 (ga1) and W22 (Ga1) whereas 20 differentially expressed proteins were identified from the pistil of W22 (Ga) and W22 (Ga1). However, in pollen, 2 proteins were identified only in the W22 (ga1) and 12 proteins only in the W22 (Ga1) whereas 10 proteins were confirmed from the both of W22 (ga1) and W22 (Ga1). In contrary, 10 proteins were appeared only in the pistil of W22 (ga1) and 7 proteins from W22 (Ga1) while 3 proteins confirmed in the both of W22 (ga1) and W22 (Ga1). Moreover, the identified proteins were generally involved in hydrolase activity, nucleic acid binding and nucleotide binding. These results help to reveal the mechanism of gametophytic factors and provide a valuable clue for the pollen and pistil research in maize. In addition, it might provide a comprehensive insight on the proteins that were involved in the regulation of pollen-pistil interaction.

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Thermophysical Properties of the Soybean Curd and Prediction of its Thermal Conductivity 1 Measurement of Thermophysical Properties of the Soybean Curd (두부의 전열물성 및 유효열전도도의 추정 1. 두부의 전열물성의 추정)

  • KONG Jai-Yul
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.15 no.3
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    • pp.211-218
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    • 1982
  • The specific heat capacity and density of soybean oil and soy protein composing of the soybean curd were measured between $30^{\circ}C\;and\;-40^{\circ}C$. The thermal conductivity of soybean oil was measured to be 0.160 and 0.140, $W/m{\cdot}K$ at unfrozen and frozen states, respectively. The effective thermal conductivity of the soybean curd depended not only on its water content but also on its fat and protein contents.

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Polyphenol Contents and Antioxidant Activity of Extracts from Angelica dahurica Root after Different Conditions of Microwave-assisted Extraction (마이크로웨이브 추출조건에 따른 백지 추출물의 폴리페놀 함량과 항산화 작용)

  • Joo, Eun-Young;Kim, Nam-Woo
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.133-138
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    • 2008
  • This study investigated the antioxidant activities of extracts from Angelica dahurica roots after microwave-assisted extraction with different levels of energy (120, 240W) and extraction time (5, 10, 15 and 30 min). The highest extraction yield was 11.77 mg% in water at 240W for 30 min followed by 11.42 mg% in water at 120Wand 30 min. The highest total polyphenol contents was 32.36 mg/g in an ethanol extract, followed by 31.77mg/g in water extract at the same conditions of 240W, 30 min. The electron donating abilities both the ethanol extract obtained using 240W and 30 min and the water extract obtained employing 120W and 5min showed the highest values, 83.55% and 82.49% respectively at a concentration 1.0mg/mL. The highest superoxide dismutase (SOD)-like activity was 14.16% in ethanol extract at 120Wand 15min, followed by 13.22% in the water extract at 120W and 5 min. The best extraction yield and polyphenol content after microwave-assisted extraction were achieved with 240W and 30 min using water. The best condition for extraction of electron donating ability and SOD-like activity from A dahurica roots were 120W and 5 minutes using water.

The Mechanical Properties of Concrete Usinga Large Amount of Fly Ash for Replacement Method (치환방법에 따른 플라이애쉬를 다량 치환한 콘크리트의 공학적 특성)

  • Kim, Tae-Wan;Kim, Tae-Sik;Jin, Chi-Sup
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.10 no.6
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    • pp.105-113
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    • 2006
  • This paper presents the results of an experimental investigation carried out to evaluate the mechanical properties of concrete mixtures in which fine(S) and coarse(G) aggregate was fully replaced with fly ash(FA). And flowability reduction problem in a large amount of fly ash concrete settled addition water($W_f$) in concrete mixture. In the test, water-cement ratio($W_c/C$) was 0.35, 0.45, and water-fly ash ratio($W_f/FA$) was 0.35, 0.45. The fly ash replacement is two different method of P and Q. The P method is mix property that the fly ash and addition water($W_f$) weight is equal to the aggregate weight [ $FA+W_f$ = G (or S)]. The Q method is mix property that fly ash is equal to aggregate weight, and added addition water($W_f$) [$FA+W_f$ > G (or S)]. Test were performed for properties of fresh concrete and compressive strength were determined at 3, 7, 28 and 91 days. The result, compressive strength was improvement that $W_c/C=0.35$, $W_f/FA=0.35$ and fine aggregate replacement in P method series than others. The flowability at Q method was improvement result than P method, but compressive strength was not. Test results indicate significant improvement in the strength properties and flowability of plain concrete by the inclusion of fly ash as fully replacement of fine and coarse aggregate, and can be effectively the fly ash replacement method.

The Fundamental Properties of Alkali-Activated Slag Cement (AASC) Mortar with Different Water-Binder Ratios and Fine Aggregate-Binder Ratios (물-결합재 비와 잔골재-결합재 비에 따른 알칼리 활성화 슬래그 모르타르의 기초특성)

  • Kim, Tae-Wan;Hahm, Hyung-Gil;Lee, Seong-Haeng;Eom, Jang-Sub
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.17 no.5
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    • pp.77-86
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    • 2013
  • This study investigates the fundamental properties of the water-binder (W/B) ratio and fine aggregate-binder (F/B) ratio in the alkali-activated slag cement (AASC) mortar. The W/B ratios are 0.35, 0.40, 0.45, and 0.50, respectively. And then the F/B ratios varied between 1.00 and 3.00 at a constant increment of 0.25. The alkali activator was an 2M and 4M NaOH. The measured mechanical properties were compared, flow, compressive strength, absorption, ultra sonic velocity, and dry shrinkage. The flow, compressive strength, absorption, ultra sonic velocity and dry shrinkage decreased with increases W/B ratio. The compressive strength decreases with increase F/B ratio at same W/B ratio. Also, at certain value of F/B ratio significant increase in strength is observed. And S2 (river sand 2) had lower physical properties than S1 (river sand 1) due to the fineness modulus. The results of experiments indicated that the mechanical properties of AASC depended on the W/B ratio and F/B ratio. The optimum range for W/B ratios and F/B ratios of AASC is suggested that the F/B ratios by 1.75~2.50 at each W/B ratios. Moreover, the W/(B+F) ratios between 0.13 and 0.14 had a beneficial effect on the design of AASC mortar.

A Study of the Fiber Fuse in Single-mode 2-kW-class High-power Fiber Amplifiers (단일 모드 2 kW급 고출력 광섬유 증폭기 내의 광섬유 용융 현상에 관한 연구)

  • Lee, Junsu;Lee, Kwang Hyun;Jeong, Hwanseong;Kim, Dong Jun;Lee, Jung Hwan;Jo, Minsik
    • Korean Journal of Optics and Photonics
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    • v.31 no.1
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    • pp.7-12
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    • 2020
  • In this paper, we experimentally investigate the fiber fuse in single-mode 2-kW-class high-power fiber amplifiers, depending on the cooling method at the splicing point. We measured the temperature of the splicing point between the pump-signal combiner and gain fiber as a function of laser output power. The temperature of the splicing point increased from 20 to 32℃ with a slope of 0.01℃/W, up to 1.2 kW of laser output power. At higher powers the temperature of the splicing point increased dramatically, with a slope of 0.08℃/W. After that, the fiber amplifier was destroyed during operation at 1.96 kW of output power by fiber fuse. The bullet shape, a common feature of fiber fuse, was observed in the damaged passive fiber core of the pump-signal combiner. Later, we adopted an improved water-cooled cold plate to increase the cooling efficiency at the splicing point, and investigated the laser output power. The temperature at the splicing point was 35.8℃ with a temperature-rise slope of 0.007℃/W at the maximum output power of 2.05 kW. The beam quality M2 was measured to be less than 1.3, and the output beam's profile was a stable Gaussian shape. Finally, neither fiber fuse nor mode instability was observed in the fiber amplifier at the maximum output power of 2.05 kW.

Diversity and Succession of the Bacterial Community during the Initial Fermentation Period in Modernized Soy Sauce (Ganjang) (개량식 간장의 발효 초기 단계에서의 미생물 다양성 및 천이에 관한 연구)

  • Ho Jin Jeong;Gwangsu Ha;Jungmi Lee;Yeji Song;Do-Youn Jeong;Hee-Jong Yang
    • Journal of Life Science
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    • v.33 no.6
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    • pp.481-489
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    • 2023
  • The taste and quality of soy sauce, a fermented liquid condiment, is greatly influenced by microbial metabolism during fermentation. To investigate the microbiological characteristics of ganjang during the initial fermentation process, we prepared meju (fermented soybean) blocks fermented with starter cultures and solar salts and analyzed the microbial community quantitively using 16S rRNA gene profiling from ganjang that had been fermented over a five-week period. The ganjang samples were collected and analyzed after soaking for week one (1W), three (3W), and five (5W) weeks. We found that Halomonadaceae was significantly higher in the 1W group (89.83%) than the 3W and 5W groups (14.46%, and 13.78%, respectively). At a species level, Chromohalobacter beijerinckii and Chromohalobacter canadensis were the dominant species in the 1W group but several taxa such as Bacillus subtilis, Pediococcus acidilactici, and Enterococcus faecalis were more abundant in the 3W and 5W groups. Pearson correlation analysis of the relative abundance of the bacteria showed a negative correlation between Chromohalobacter and two bacterial genera Bacillus and Enterococcus. Beta-diversity showed a statistical distinction between the 1W and the 3W and 5W groups, while no significance was evident between the 3W and 5W groups. Linear discriminant effect size analysis was used to identify biomarkers and significant differences in the relative abundance of several halophilic bacteria, Bacillus sp. and lactic acid bacteria at 1W, 3W, and 5W, recpectively, which indicates the important role of the bacterial community at these time points.