• 한국식품영양과학회지
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• 제38권8호
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• pp.1050-1061
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• 2009

본 연구는 대구 경북지역의 뇌졸중 환자들의 에너지 및 영양소 섭취실태를 측정하고, 에너지 및 영양소 섭취수준에 따른 뇌졸중 발생의 상대위험도를 분석하고자 수행되었다. 조사대상자는 경북대학교 병원에서 뇌졸중으로 처음 진단받은 후 입원한 환자 중 의사소통이 가능한 100명을 환자군으로 하였고 동일한 지역에서 뇌졸중 병력이 없고 환자군과 연령이 비슷한 지역사회 주민 150명을 대조군으로 하였다. 조사는 반정량적 식품섭취빈도 조사지를 이용하여 조사자와 직접 면접을 통하여 2004년 7월부터 2005년 2월 사이에 이루어졌다. 본 연구의 결과를 요약하면 다음과 같다. 뇌졸중 환자군과 대조군의 1일 평균 에너지와 영양소 섭취량을 성별로 비교하면 남자의 경우 환자군이 대조군에 비해 에너지와 모든 열량 영양소, 비타민 $B_1$, $B_6$, 나이아신, 엽산, 비타민 E, 인, 칼륨, 아연, 식이섬유의 섭취량과 단백질 및 지방 에너지 섭취비율이 유의하게 높았다. 여자의 경우 환자군은 대조군에 비해 지방, 비타민 C, 엽산, 비타민 E, 철분 및 식이섬유의 섭취량이 유의하게 낮았고 탄수화물 에너지 비율이 유의하게 높았다. 에너지 및 영양소별 섭취수준과 관련된뇌졸중 발생 상대위험도를 성별로 보면, 남자는 에너지, 단백질, 탄수화물, 비타민 $B_1$과 E, 나이아신, 아연, 총지방산, 단일불포화 지방산, 다가 불포화지방산 그리고 n-6 지방산의 높은 섭취수준이 뇌졸중 발생의 상대 위험도를 유의하게 높이는 것으로 나타났다. 여자의 경우 지방, 비타민 A, 비타민 $B_2$와 $B_6$, 나이아신, 비타민 C와 E, 철, 나트륨과 칼륨 및 셀레늄, 모든 지방산들, 그리고 콜레스테롤, 타우린, 식이섬유의 높은 섭취수준이 뇌졸중의 발생 위험도를 유의하게 낮추는 것으로 나타났다. 영양소 중 나이아신, 비타민 E, 지방산들은 성별에 따라 위험도에 미치는 영향이 상반되게 나타났다. 본 연구는 조사대상자의 규모가 작고 특정지역에 국한 하여 조사가 이루어졌다는 제한점을 가지고 있다. 따라서 성별에 따라 상반되는 결과를 보인 영양소들에 대해서는 향후 조사지역을 광역화하고, 보다 대규모의 대상자 집단에 대해 심도 있는 조사를 통해 재확인할 필요가 있다고 본다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6249-6256
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• 2013

One-carbon metabolism plays an important role in colorectal carcinogenesis. Meta-analyses have suggested protective associations of folate and vitamin $B_6$ intakes with colorectal cancer primarily based on studies in Caucasians, and genetic polymorphisms pertaining to the folate metabolism have been a matter of interest. Less investigated are the roles of methionine synthase (MTR) and thymidylate synthetase (TS) polymorphisms in colorectal carcinogenesis. In a study of 816 cases and 815 community controls in Japan, we investigated associations of dietary intakes of folate, methionine, vitamin $B_2$, vitamin $B_6$, and vitamin $B_{12}$ with colorectal cancer risk. The associations with MTR 2756A>G, MTRR 66A>G, and TSER repeat polymorphism were examined in 685 cases and 778 controls. Methionine and vitamin $B_{12}$ intakes were inversely associated with colorectal cancer risk, but the associations were totally confounded by dietary calcium and n-3 fatty acids. The other nutrients showed no association with the risk even without adjustment for calcium and n-3 fatty acids. The TSER 2R allele was dose-dependently associated with an increased risk. The MTR and MTRR polymorphisms were unrelated to colorectal cancer risk. There was no measurable gene-gene or gene-nutrient interaction, but increased risk associated with the TSER 2R allele seemed to be confined to individuals with high folate status. This study does not support protective associations for folate and vitamin $B_6$. The TSER 2R allele may confer an increased risk of colorectal cancer. The role of the TSER polymorphism in colorectal carcinogenesis may differ by ethnicity.

• 한국식품영양과학회지
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• 제26권6호
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• pp.1058-1062
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• 1997

The objective of this was to investigate the technical feasibility of producing toxicant-free by germination. To this end, rapeseed(Brassica napus L.) was germinated at $25^{\circ}C$ for 120 hours, and the chemical compositions of amino acids and vitamins were determinated in every 24 hours during germination. The results obtained are summarized as follows: Before germination, rapeseed contained 5.4g/16g N of glutamic acid and high percentage of the other amino acids in order of Asp>Leu>His>Pro>Arg>Lys>Gly>Ser>Ala>Val. The amino acids were gradually decreased until 96 hours during germination had tendency to show a slight increase in 120 hours. Vitamin B$_1$, B$_2$and C contents in rapeseed before germination were found to be 0.11, 0.21 and 3.72mg% respectively, and the vitamin E was 423ug/g. The vitamin C greatly increased in 72 hours during germination, while the vitamin B group was drastically decreased in 72 hours. The results obtained by this method clearly demonstrate that germination process is very effective to the removal of toxicants in rapeseed.

• 한국식품영양과학회지
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• 제44권12호
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• pp.1856-1864
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• 2015

본 연구는 춘천지역 대학생 306명(남 175명, 여 131명)을 대상으로 성별에 따른 영양소 섭취 상태를 알아보았으며, 그 결과는 다음과 같다. 평균 나이는 남학생이 $21.1{\pm}2.9$세, 여학생이 $20.3{\pm}2.2$세였고(P<0.05), 평균 신장과 체중은 남녀 각각 $175.2{\pm}6.2cm$와 $68.2{\pm}9.9kg$, $161.7{\pm}5.2cm$와 $55.1{\pm}6.5kg$으로 나타났으며(P<0.001), 평균 BMI는 남녀 각각 $22.2{\pm}2.8$과 $21.1{\pm}2.1$로 나타났다(P<0.001). 끼니별 결식률은 남녀 모두 아침결식률(31.7%, 29.2%)이 가장 높게 나타났다. 성별에 따른 영양소 섭취 상태를 알아본 결과, 1일 평균 에너지, 탄수화물, 단백질, 지방은 남학생이 여학생보다 유의적으로 높게 섭취하였다(P<0.001). 또한 비타민 A와 C를 제외한 비타민 $B_1$(P<0.001), 비타민 $B_2$(P<0.01), 나이아신(P<0.001), 비타민 $B_6$(P<0.001), 비타민 E(P<0.001), 엽산(P<0.01), 칼슘(P<0.05), 인(P<0.001), 철분(P<0.001), 아연(P<0.001), 나트륨(P<0.001), 콜레스테롤(P<0.001)도 남학생이 유의적으로 높게 섭취하였다. 주중과 주말의 영양소 섭취 상태는 남학생이 1일 평균 에너지(P<0.01), 탄수화물(P<0.01), 단백질(P<0.05), 지방(P<0.01), 비타민 $B_1$(P<0.01), 나이아신(P<0.01), 비타민 $B_6$(P<0.01), 비타민 E(P<0.01), 철분(P<0.05), 아연(P<0.01), 나트륨(P<0.05)을, 여학생은 지방(P<0.05), 비타민 E(P<0.05), 칼슘(P<0.01), 철분(P<0.01)을 주말에 비해 주중에 유의적으로 높게 섭취하였다. 에너지 섭취량에 대한 탄수화물, 단백질, 지방의 섭취비율은 남학생이 57.8:15.3:26.8, 여학생이 58.5:15.1:26.2로 나타나면서 서로 유사한 결과를 보였다. 성별에 따른 한국인 영양섭취기준에 대한 섭취비율을 보면 권장섭취량과 충분섭취량 이상 섭취한 영양소는 남학생의 경우 단백질, 비타민 $B_1$, 인, 철분, 나트륨이었으며, 여학생은 단백질, 비타민 A, 인, 나트륨으로 나타났다. 남학생의 에너지 필요추정량에 대한 섭취비율은 69%, 여학생은 70%였으며, 남학생은 비타민 $B_2$, 비타민 C, 칼슘을, 여학생은 비타민 C, 철분, 칼슘을 권장섭취량의 80% 이하로 섭취하고 있었다. 주중과 주말의 영양섭취기준에 대한 섭취비율을 살펴보면 남학생은 비타민 $B_6$와 아연을, 여학생은 칼슘과 철분을 주중에 비해 주말에 상대적으로 부족하게 섭취하였다(P<0.01). 성별에 따른 EAR 미만 섭취자 비율은 남학생이 칼슘(81.7%), 비타민 $B_1$(69.4%), 비타민 C(64.9%), 비타민 $B_6$(53.7%)를, 여학생은 칼슘(75.4%), 비타민 C(64.6 %), 철분(60.8%), 비타민 $B_6$(58.5%), 비타민 $B_2$(55.8%), 나이아신(53.5%), 엽산(51.9%)이 50%를 넘어 영양소의 섭취량이 매우 낮음을 알 수 있었다. 이상의 연구 결과에서 대학생들의 높은 아침결식률, 남학생이 여학생에 비해 높은 영양 섭취를 보이고 있었으나 전반적으로 대학생들의 에너지 섭취 수준은 낮았으며 특정 영양소, 특히 칼슘, 비타민 C, 철분, 비타민 $B_2$ 등의 영양 섭취 상태가 나쁜 것으로 나타났다. 또한 아침결식률과 영양 섭취 상태는 주중보다는 주말에 문제가 있는 것으로 나타나 불규칙한 생활 패턴에 따른 식습관 개선이 시급한 상황이다. 따라서 규칙적인 식생활 및 균형 잡힌 식습관 등에 대한 정확한 이해와 함께 식태도가 긍정적인 방향으로 변화되어 대학생들이 건강과 영양 상태 향상에 도움이 되는 행동을 자발적으로 습득할 수 있도록 영양교육의 필요성이 절실히 요구되는 바이다. 한편 본 연구는 표본의 대상이 춘천지역 일부 대학생이기에 연구 결과를 일반화시키는 데 어려움이 있다. 또한 영양소 섭취 상태의 분석 자료로 사용된 24시간 회상법은 자기기입방법으로 정확한 섭취량을 측정하기 어려운 점이 있어 본 연구의 영양소 섭취 상태와 국민건강영양조사 결과에 차이를 보인 것으로 생각된다. 그러나 본 연구는 개인의 영양 상태 평가가 아닌 집단 간의 비교이고, 개인별 일일 식품 섭취의 편차를 최소화하기 위해 주중과 주말이 포함된 4일 간의 조사를 실시하였기에 다른 지역 및 연령대와의 비교나 특성 연구 시 기초자료로 활용될 수 있을 것이다.

• 한국식품영양학회지
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• 제33권4호
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• pp.390-398
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• 2020

The purpose of this study was to determine the contents of water-soluble vitamins B₁ (thiamine), B₂ (riboflavin), B₃ (niacin) and B₁₂ (cyanocobalamin) in seafood-based dishes by using the high-performance liquid chromatography with the ultra violet and fluorescence detector. The vitamin B₁, B₂, B₃ and B₁₂ contents were analyzed in 29 seafood-based dishes. The method validation was performed on the method to determine the linearity, accuracy, limits of quantification, limits of detection and precision for vitamin B₁, B₂, B₃ and B₁₂. An excellent linearity range (R²=0.999~1.000) in the calibration curve for the water-soluble vitamins was observed. All analytical methods for the water-soluble vitamins showed the acceptable accuracy (89.4~119.7% recovery) and the precision (0.6~4.8% repeatability and 1.0~4.2% reproducibility). The highest content of vitamin B₁ was 2.646 mg/100 g in the kkongchi-jorim, and the highest contents of vitamin B₂, B₃ and B₁₂ were 0.370 mg/100 g, 10.971 mg/100 g, and 17.193 ㎍/100 g, respectively, in the kkongchi-gui. Our results provide reliable data on the contents of the water-soluble vitamins of seafood-based dishes in Korea.

• 한국응용과학기술학회지
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• 제4권1호
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• pp.1-8
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• 1987

This experiment is carried out to study influence of vitamin $B_6$ on serum lipids of rat fed with oxidized oil by heat. The experimental animals use 35 male Sprague-Dawley rats weighing 100 ${\pm}$ 2g. They are divided into 5 groups and fed to experimental diets which are added to 10%, 15%, 20% oxidized oil by heat in addition to 0.01%, 0.04%, 0.06% vitamin $B_6$. Oxidized oil by heat is prepared from the soybean oil by heating at $180^{\circ}C$ for 50 hours. After feeding for 4 weeks, lipid concentrations of sera are as follows; 1. Body weights are higher in experimental diet groups than control diet group. 2. Liver weights are higher in experimental diet groups than control diet group, but spleen weights are lower in experimental diet groups than control diet group. 3. The contents of serum total cholesterol, free cholesterol, HDL-cholesterol are lower in experimental diet groups than control diet group. 4. The contents of serum triglyceride are significantly higher in experimental diet groups than control diet group. 5. The contents of serum phospholipid are lower in experimental diet groups than control diet group.

• Journal of Microbiology and Biotechnology
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• 제33권5호
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• pp.644-655
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• 2023

Safety assessment and functional analysis of probiotic candidates are important for their industrial applications. Lactiplantibacillus plantarum is one of the most widely recognized probiotic strains. In this study we aimed to determine the functional genes of L. plantarum LRCC5310, isolated from kimchi, using next-generation, whole-genome sequencing analysis. Genes were annotated using the Rapid Annotations using Subsystems Technology (RAST) server and the National Center for Biotechnology Information (NCBI) pipelines to establish the strain's probiotic potential. Phylogenetic analysis of L. plantarum LRCC5310 and related strains showed that LRCC5310 belonged to L. plantarum. However, comparative analysis revealed genetic differences between L. plantarum strains. Carbon metabolic pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database showed that L. plantarum LRCC5310 is a homofermentative bacterium. Furthermore, gene annotation results indicated that the L. plantarum LRCC5310 genome encodes an almost complete vitamin B₆ biosynthetic pathway. Among five L. plantarum strains, including L. plantarum ATCC 14917^T , L. plantarum LRCC5310 detected the highest concentration of pyridoxal 5'-phosphate with 88.08 ± 0.67 nM in MRS broth. These results indicated that L. plantarum LRCC5310 could be used as a functional probiotic for vitamin B₆ supplementation.

• 대한가정학회지
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• 제20권2호
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• pp.91-102
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• 1982

This study was designed to find out the nutritional defect of general Korean diet and the effective way of nutritional supplementation. Seventy weanling Sprague-Dawley male rats weighing 51.8$\pm$0.9g were blocked into ten groups and fed ten different diets ad libitum for eight weeks: Standard groups(st gp) was given 72% sugar-20% casein diet: Cereal-vegetable group(c-v gp) was fed cereal-vegetable diet(c-v diet) composed of rice, barley, soybean, spinach and cabbage: the other eight groups were fed c-v diets supplemented with casein, vitamin $B_{2}$, calcium, vitamin A, vitamin B2 and A, vitamin A and calcium, vitamin $B_{2}$ and calcium, vitamin A and $B_{2}$and calcium, respectively, on the basis of each nutrients content of standard diet. The results were as follows: 1. Food intakes and body weight gains in all the experimental groups were significantly lower than st gp. Among experimental groups, casein gp and vit B2 gp tended to gain more body weights than c-v gp. 2. Through all the experimental period, F.E.R., pp.E.R., and NDPcal% did nod show significant differences among all the experimental groups and st gp. 3. The weights of liver, kidney, and gastrocnemius were significantly lower in all the experimental groups as compared with st gp. But brain and sex organ weights did not show differences among all the groups. 4. Nitrogen contents of total carcass, liver, and gastrocnemius in all the experimental groups tended to be increased as compared with st gp, and among experimental groups, they tended to be increased by casein supplementation and decreased by ca supplementation. 5. Apparent nitrogen digestibility, urinary and fecal nitrogen excretion, the amount of nitrogen retained, and N.P.U. did not show any significant differences among all the groups. 6. Serum total protein concentrations did not show any significant differences among all the groups.

• 대한지역사회영양학회지
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• 제7권2호
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• pp.167-176
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• 2002

To assess the dietary fiber intake of preschool children in Busan and to evaluate the relationship between of the intake of dietary fiber and nutrient intake. Nutrient intake using 24 hour recall, and total dietary fiber (TDF) intake based on tables of TDF of common Korean floods developed by the modified Prosky Method, were estimated for 176 preschool children. The mean daily intakes of TDF, and TDF after adjusting energy intake, were 10.20 g and 7.69 g/1,000 kcal, respectively. The mean daily intakes of TDF for children aged 1-3 and 4-6 years were 9.20 g and 11.08 g, respectively. The range of TDF intake was 1.86 to 22.16 g. The major sources of TDF were cereals (31.0%), vegetables (18.9%) and fruits (11.9%). The TDF intake showed positive correlations with nutrient adequacy ratios (NAR) of iron and Vitamin $B_1$, (p < 0.05, p < 0.05). The TDF intake per 1,000 kcal showed negative correlations with the NARs of protein, calcium, phosphate, iron, Vitamin A, vitamin $B_1$, Vitamin $B_2$, and niacin (p < 0.001, p < 0.001, p < 0.001, p < 0.05, p < 0.001, p < 0.001, p < 0.001, p < 0.001) and with the mean adequacy ratio (MAR, p < 0.001). When children were stratified into quartiles ($Q_1-Q_4$) on the basis of their fiber intake per 1,000 kcal, their NARs for calcium, phosphate, iron, Vitamin A, Vitamin $B_1, Vitamin $B_2$ and niacin (p < 0.05, p < 0.05, p < 0.001, p < 0.05, p < 0.05, p < 0.001, p < 0.001), and their MAR (p < 0.001) were significantly lower in the children with higher fiber intake per 1,000 kcal (the upper quartile). The NARs fur calcium (0.63), iron (0.60), Vitamin A (0.66), Vitamin $B_2$(0.74), niacin (0.64), Vitamin C (0.65) and the MAR (0.74) were lower than 0.75 in the children with fiber intakes of more than 9.25 g per 1,000 local (0,), the highest fiber intake per 1,000 kcal. Based on these results, the mean TDF intake of children was higher than the age (yr)+5g , the minimum recommended level for American children. Meals with a fiber intake of more than 9.25 g per 1,000 local ($Q_4$) could cause a decreased nutritional status for minerals and vitamins. The result of this study could contribute to the establishment of Recommended Dietary Allowances (RDA) for dietary fiber for Korean Preschool children.

• Journal of Nutrition and Health
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• 제11권4호
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• pp.39-48
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• 1978

The present study is concerned with nutritional status and palatability of the packed-lunch in elementary school children in Daejeon city. Rice and side dishes in the lunch box were weighed and the contained nutrients were analyzed by the Food Composition Table. The result of survey is as follows; 1) The means of nutritional values of 523 lunch boxes were calorie 562.48 Cal. percentage of the recommended dietary allowances.(79.11%), protein 19.16gr. (88.43%), animal protein 6.03gr. (83.50%), calcium 110.75mg (48.15%), iron 2.20mg. (56.15%), Vitamin A 328.16 I.U.. (49.22%), Vitamin $B_1$ 0.22mg. (59.45%), Vitamin $B_2$ 0.14mg (32.56%), Niacin 3.20mg. (68.08%), Vitamin C 9.79mg. (73.44%). All the nutrients are under the recommended dietary allowances. 2) The correlation between the boys and girls in the intake of protein, calcium, iron, Vitamin A, Vitamin $B_1$, Niacin index revealed statistical significancy at the level of P<0.01. 3) According to the intake of calorie, the ratio of carbohydrates, protein and fat is 78 : 14 : 8, which is excessive meal of carbohydrate. 4) Among the total intake of the protein consists of 30.20% for the boys and 32.90% for the girls. 5) The most favorite food for boys and girls from the packed-lunch is kimchi, egg, ham, sausage, cuttle-fish, laver and beef, and pork and pickles are most undesirable items. 6) The most favorite snack is bread, cake, ice cream, chocolate, fruits and milk. 7) Both boys and girls like Kimchi, fried and saute foods but dislike Namul (seasoned green vegetables) the most. 8. The side dishes of the packed·lunch lack in variety of cooking method.