• Nutrition Research and Practice
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• 제8권6호
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• pp.688-694
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• 2014

BACKGROUND/OBJECTIVES: Recent research regarding vitamin $B_6$ status including biochemical index is limited. Thus, this study estimated intakes and major food sources of vitamin $B_6$; determined plasma pyridoxal 5'-phosphate (PLP); and assessed vitamin $B_6$ status of Korean adults. MATERIALS/METHODS: Three consecutive 24-h diet recalls and fasting blood samples were collected from healthy 20- to 64-year-old adults (n = 254) living in the Seoul metropolitan area, cities of Kwangju and Gumi, Korea. Vitamin $B_6$ intake and plasma PLP were analyzed by gender and by vitamin $B_6$ supplementation. Pearson's correlation coefficient was used to determine associations of vitamin $B_6$ intake and plasma PLP. RESULTS: The mean dietary and total (dietary plus supplemental) vitamin $B_6$ intake was $1.94{\pm}0.64$ and $2.41{\pm}1.45mg/day$, respectively. Median (50th percentile) dietary intake of men and women was 2.062 and 1.706 mg/day. Foods from plant sources provided 70.61% of dietary vitamin $B_6$ intake. Only 6.3% of subjects consumed total vitamin $B_6$ less than Estimated Average Requirements. Plasma PLP concentration of all subjects was $40.03{\pm}23.71nmol/L$. The concentration of users of vitamin $B_6$ supplements was significantly higher than that of nonusers (P < 0.001). Approximately 16% of Korean adults had PLP levels < 20 nmol/L, indicating a biochemical deficiency of vitamin $B_6$, while 19.7% had marginal vitamin $B_6$ status. Plasma PLP concentration showed positive correlation with total vitamin $B_6$ intake (r = 0.40984, P < 0.0001). CONCLUSIONS: In this study, vitamin $B_6$ intake of Korean adults was generally adequate. However, one-third of subjects had vitamin $B_6$ deficiency or marginal status. Therefore, in some adults in Korea, consumption of vitamin $B_6$-rich food sources should be encouraged.

• 치위생과학회지
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• 제20권1호
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• pp.9-15
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• 2020

Background: Although the consumption of vitamin beverages has increased because of the recent interest in health and beauty, guidelines addressing appropriate consumption habits are lacking. Thus, the aim of this study was to investigate the erosive potential of several vitamin beverages and to propose guidelines for the appropriate intake of these drinks. Methods: Five vitamin beverages were selected after a pre-investigation of the current beverage market. Coca-Cola and mineral water were selected as the control beverages. The pH of the beverages was measured with a calibrated pH meter, and the titratable acidity (TA) was determined by using 1 M sodium hydroxide to reach pH 5.5 (TA5.5) and 7.0 (TA7.0). The screening method suggested by the International Organization for Standardization was used to measure pH variation (ΔpH) by using an under-saturated hydroxyapatite solution to determine the difference between the initial and final pH of the screening solution. All measurements were performed in triplicate. Results: All vitamin beverages tested in this study exhibited a low pH (2.53~2.99), similar to Coca-Cola, which is known to be a highly acidic beverage. The highest TA5.5 and TA7.0 values of the vitamin beverages were 7.03 ml and 8.81 ml, respectively. The largest change in pH determined by using the screening solution was found in Bacchus D (ΔpH 1.44±0.05). The mean ΔpH of the vitamin beverages was 1.12±0.29, which was higher than that of Coca-Cola (positive control, ΔpH 0.58±0.05). Conclusion: Vitamin beverages exhibited an erosive potential capable of damaging enamel surfaces. Therefore, the frequency of vitamin beverage intake should be limited, and individuals consuming these drinks should try to restore normal oral pH as quickly as possible.

• Asian-Australasian Journal of Animal Sciences
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• 제29권5호
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• pp.681-688
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• 2016

The present study was conducted to compare the supplementation of natural (D-${\alpha}$-tocopherol) and synthetic (DL-${\alpha}$-tocopherol acetate) vitamin E on the growth performance, meat quality, muscular antioxidant capacity and genes expression related to oxidative status of broilers. A total of 144 1 day-old Arbor Acres broiler chicks were randomly allocated into 3 groups with 6 replicates of 8 birds each. Birds were given a basal diet (control group), and basal diet supplemented with either 20 IU D-${\alpha}$-tocopherol or DL-${\alpha}$-tocopherol acetate for 42 days, respectively. The results indicated that treatments did not alter growth performance of broilers (p>0.05). Compared with the control group, concentration of ${\alpha}$-tocopherol in the breast muscle was increased by the supplementation of vitamin E (p<0.05). In the thigh, ${\alpha}$-tocopherol content was also enhanced by vitamin E inclusion, and this effect was more pronounced in the natural vitamin E group (p<0.05). Vitamin E supplementation increased the redness of breast (p<0.05). In the contrast, the inclusion of synthetic vitamin E decreased lightness of thigh (p<0.05). Dietary vitamin E inclusion reduced drip loss at 24 h of thigh muscle (p<0.05), and this effect was maintained for drip loss at 48 h in the natural vitamin E group (p<0.05). Broilers given diet supplemented with vitamin E showed decreased malondialdehyde (MDA) content in the breast (p<0.05). Additionally, natural rather than synthetic vitamin E reduced MDA accumulation in the thigh (p<0.05). Neither natural nor synthetic vitamin E supplementation altered muscular mRNA abundance of genes related to oxidative stress (p>0.05). It was concluded that vitamin E supplementation, especially the natural vitamin E, can enhance the retention of muscular ${\alpha}$-tocopherol, improve meat quality and muscular antioxidant capacity of broilers.

• Asian-Australasian Journal of Animal Sciences
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• 제19권4호
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• pp.563-567
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• 2006

Two experiments were conducted to compare the effect of various vitamins on performance and digestibility in growing pigs. In experiment 1, a total of 54 pigs ($L{\times}Y{\times}D$, $42.73{\pm}2.40kg$) were assigned to three treatments in a randomized complete block design with three replicates (6 pigs/pen) for 40 days. The three dietary treatments were: 100% fat-soluble vitamins (FSV) and water-soluble vitamins (WSV); 150% FSV and 100% WSV of NRC (1998); and 100% FSV and 150% WSV of NRC (1998). In experiment 2, a total of 180 pigs ($L{\times}Y{\times}D$, $28.20{\pm}3.05 kg$) were assigned to four treatments in a completely randomized design with three replicates for four weeks (15 pigs/pen). The four dietary treatments were, 150% vitamin A and 100% other vitamins, 150% vitamin D and 100% other vitamins, 150% vitamin E and 100% other vitamins, and 150% vitamin K and 100% other vitamins. In experiment 1, there were significant differences in growth performance and digestibility of nutrients among the treatments. The ADG, ADFI and FCR of pigs fed 150% FSV diet were better (p<0.05) than those fed the control diet. However, there were no differences (p>0.05) in ADG, ADFI and FCR between pigs fed the control and 150% WSV diets. Digestibilities of dry matter, gross energy and calcium were improved in 150% FSV treatment group compared with control (p<0.05). However, the improvement was similar when compared with 150% WSV except for Ca. In experiment 2, there were no differences (p>0.05) in ADG, ADFI and FCR and nutrient digestibility between the fat-soluble vitamin treatments when fed at the 150% level. In conclusion, growing pigs were more responsive to additional fat-soluble vitamin supplements over the requirements suggested by NRC (1998) than to water-soluble vitamin supplements as measured by growth performance and digestibility of nutrients.

• 한국가금학회지
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• 제34권3호
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• pp.157-163
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• 2007

비타민 E 100 IU, selenium yeast로 셀레늄을 0.9 ppm과 비타민 E를 50, 100 및 150 IU로 복합 첨가하였을 때 산란계의 산란율, 난중, 1일 산란량, 사료 섭취량, 사료 요구율, 난질, 계란의 저장성 및 계란 내 셀레늄 축적에 미치는 영향을 조사한 결과를 요약하면 다음과 같다. 시험 전 기간의 산란율과 1일 산란량은 비타민 E 100 IU 첨가구와 유기태 셀레늄 0.9 ppm과 비타민 E 50 IU 복합 첨가구가 대조구에 비하여 높았으며(P<0.05), 사료 섭취량과 사료 요구율은 대조구를 포함한 모든 처리구에서 차이가 없었다. Haugh unit는 5주에 비타민 E 100 IU 첨가구와 유기태 셀레늄 0.9 ppm과 비타민 E 50 IU 복합 첨가구가 대조구를 포함한 다른 처리구에 비하여 높았으며(P<0.05), 난황색은 1주에 유기태 셀레늄 0.9 ppm에 비타민 E 50 IU와 100 IU를 복합 첨가한 구가 대조구를 포함한 다른 처리구에 비하여 높았다(P<0.05). 계란의 셀레늄 함량은 유기태 셀레늄 0.9 ppm에 비타민 E 50, 100 및 150 IU를 복합 첨가한 모든 처리구가 대조구와 비타민 E 100 IU 첨가구에 비하여 높게 나타났다(P<0.05). 계란 난황 내 ACL은 유기태 셀레늄 0.9 ppm와 비타민 E 150 IU 복합 첨가구가 대조구를 포함한 다른 처리구에 비하여 높게 나타났다(P<0.05). 계란의 저장 기간별 Haugh unit는 대조구를 포함한 모든 처리구에서 저장기간이 경과할수록 감소하였으나, 셀레늄 0.9 ppm과 비타민 E 150 IU를 복합 첨가한 경우에는 저장 1일차에서 5일차까지의 Haugh unit에 유의적인 차이가 없었다. 이상의 결과를 종합하면 산란계 사료에 유기태 셀레늄 0.9 ppm 첨가하면서 비타민 E를 50, 100 및 150 IU를 복합 첨가시 산란율은 유기태 셀레늄 0.9 ppm 첨가하면서 비타민 E를 50 IU 복합 첨가하면 개선되었으며, 난황내 지용성 항산화물과 계란의 저장성은 유기태 셀레늄 0.9 ppm 첨가하면서 비타민 E를 150 IU 복합 첨가하면 향상되었다.

• 대한지역사회영양학회지
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• 제15권4호
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• pp.460-474
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• 2010

This study was performed to assess the physical activity, food habit and nutrient intakes by gender and age groups in 193 adults aged 20-59 years (84 men and 109 women) in Pusan. Data for physical activity and dietary survey was assessed by a questionnaire and 24hr recall method. The mean BMIs of men and women were 24.0 and 22.2 respectively and BMI of women in the 20-29 years group (20.9) was significantly lower than that of women in the 30-49 and 50-59 years group (22.7, 23.2) (p < 0.01). 56.0% for men and 44.0% for women exercised regularly. The mean exercise duration per once of men (69.7 minutes) was significantly higher than that of women (52.4minutes) (p < 0.01). The mean exercise duration per day was 36.0 minutes for men and 29.9 minutes for women. 67.9% for men and 78.0% for women often skipped meals and 68.4% for men and 69.4% for women skipped breakfast in the main. The mean energy intake of men was 2067.2 kcal and that of women was 1783.1 kcal comprised of 87.2% and 92.1% of the Estimated Energy Requirements (EER). The mineral intakes of men and women were over Recommended Intake (RI) and Adequate Intake (AI) except calcium and potassium. The mean calcium intake was 88.3% for men and 84.0% for women of RI. The mean potassium intake was 63.3% for men and 59.2% for women of AI. The mean vitamin intakes of men and women were over RI and AI except vitamin C and folic acid. The mean vitamin C intake was 92.5% for men and 85.6% for women of RI. The mean folic acid intake was 76.6% for men and 70.0% for women of RI. The mean energy, protein, sodium and zinc intakes of men were significantly higher than those of women (p < 0.01, p < 0.05, p < 0.05, p < 0.05). The mean vitamin $B_6$, vitamin C and folic acid intakes of men in the 20-29 years group were significantly lower than those of men in the 30-49 and 50-59 years group (p < 0.01, p < 0.05, p < 0.05). For energy, proportions of subjects with intake levels less than 90% EER were 64.3% for men and 56.0% for women. For calcium, proportions of subjects with intake levels less than Estimated Average Requirement (EAR) were 52.4% for men and 59.6% for women. For folic acid, proportions of subjects with intake levels less than EAR were 78.6% for men and 83.5% for women. For iron and phosphorus, proportions of women (36.7%, 14.7%)with intake levels less than EAR were significantly higher than those of men (6.0%, 1.2%) (p < 0.01, p < 0.001). For men, age was positively correlated with intakes of potassium, vitamin $B_6$, vitamin C and folic acid (p < 0.05, p < 0.05, p < 0.05, p < 0.01). For men, weight showed significantly negative correlations with intakes of carbohydrate, phosphorus, potassium, zinc, vitamin $B_6$ and folic acid (p < 0.01, p < 0.05, p < 0.05, p < 0.05, p < 0.01) and BMI showed significantly negative correlations with protein, lipid, phosphorus, potassium, zinc, vitamin E and folic acid (p < 0.05, p < 0.05, p < 0.05, p < 0.05, p < 0.05, p < 0.05, p < 0.05) For men, exercise duration per once showed significantly positive correlations with intakes of calcium, phosphorus, potassium, zinc, vitamin $B_1$, vitamin $B_2$, niacin, vitamin C and folic acid (p < 0.01, p < 0.05, p < 0.01, p < 0.05, p < 0.05, p < 0.01, p < 0.01, p < 0.01, p < 0.05). Therefore, nutritional education for adult health management is needed by gender and age groups.

• Nutrition Research and Practice
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• 제5권5호
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• pp.396-403
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• 2011

Ceramides (Cer) comprise the major constituent of sphingolipids in the epidermis and are known to play diverse roles in the outermost layers of the skin including water retention and provision of a physical barrier. In addition, they can be hydrolyzed into free sphingoid bases such as $C_{18}$ sphingosine (SO) and $C_{18}$ sphinganine (SA) or can be further metabolized to $C_{18}$ So-1-phosphate (S1P) and $C_{18}$ Sa-1-phosphate (Sa1P) in keratinocytes. The significance of ceramide metabolites emerged from studies reporting altered levels of SO and SA in skin disorders and the role of S1P and Sa1P as signaling lipids. However, the overall metabolism of sphingoid bases and their phosphates during keratinocyte differentiation remains not fully understood. Therefore, in this study, we analyzed these Cer metabolites in the process of keratinocyte differentiation. Three distinct keratinocyte differentiation stages were prepared using 0.07 mM calcium (Ca$^{2+}$) (proliferation stage), 1.2 mM Ca$^{2+}$ (early differentiation stage) in serum-free medium, or serum-containing medium with vitamin C (50 ${\mu}L$/mL) (late differentiation stage). Serum-containing medium was also used to determine whether vitamin C increases the concentrations of sphingoid bases and their phosphates. The production of sphingoid bases and their phosphates after hydrolysis by alkaline phosphatase was determined using high-performance liquid chromatography. Compared to cells treated with 0.07 mM Ca$^{2+}$, levels of SO, SA, S1P, and SA1P were not altered after treatment with 1.2 mM Ca$^{2+}$. However, in keratinocytes cultured in serum-containing medium with vitamin C, levels of SO, SA, S1P, and SA1P were dramatically higher than those in 0.07- and l.2-mM Ca$^{2+}$-treated cells; however, compared to serum-containing medium alone, vitamin C did not significantly enhance their production. Taken together, we demonstrate that late differentiation induced by vitamin C and serum was accompanied by dramatic increases in the concentration of sphingoid bases and their phosphates, although vitamin C alone had no effect on their production.

• Environmental Analysis Health and Toxicology
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• 제4권3_4호
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• pp.77-86
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• 1989

Influences of u-tocopherol on the toxicity of vitamin A acetate in male rats were studied. The obtained results are as follows; 1) The administration of vitamin A acetate 500,000 IU/Kg i.p. twice at 3 days interval decreased the liver weight/body weight and increased the spleen weight/body weight, and increased activities of SGOT and alkaline phosphatase, and also increased BUN and creatinine. 2) ${\alpha}$-Tocopherol administered together with vitamin A acetate as given as the above 1) poteniated the increase of SGOT activity caused by vitamin A acetate and reduced the increase of alkaline phosphatase activity and creatinine which were caused by vitamin A acetate. 3) The administration of vitamin A acetate 500,000 IU/Kg i.p. twice a week for 4 weeks showed remarkable decrease of body weight gain and the effect of it was larger in later stage than in early. It increased significantly liver weight/body weight and further increased the activities of SGOT, SGPT and alkaline phosphatase, and showed no influnence on BUN and creatinine. 4) ${\alpha}$-Tocopherol administered together with vitamin A acetate as given as the above 3) reduced the decrease of body weight gain caused by vitamin A acetate, and potentiated remarkably the increased activities of SGOT and alkaline phosphatase which were caused by vitamin A acetate.

• Asian-Australasian Journal of Animal Sciences
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• 제16권4호
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• pp.558-564
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• 2003

A $3{\times}3$ (Fe$\times$VA) experiment with repeats was designed to study the interaction between iron and vitamin A in broilers. 504 broilers were divided into 9 groups (50% males, 50% femals), each group with 4 repeats. Iron supplemental amount was 0, 30 and 60 mg/kg; Vitamin A supplemental amount was 750, 1,500 and 2,700 IU/kg. Iron concentration in liver, serum, tibia and duodenum and vitamin A concentration in liver and serum were measured, and erythrocyte count was also observed. Results showed with the increase of dietary supplemental iron levels, vitamin A concentration in liver significantly decreased lineally (p<0.05) (0.861, 0.671, 0.639 mg/100 g at the end of 4th week; 0.900, 0.765, 0.739 mg/100 g at the end of 7th week), and vitamin A concentration in serum significantly increased lineally (p<0.05) (82.725, 97.842, $109.475{\mu}g/100mL$ at the end of 4th week; 62.288, 91.900, $95.117{\mu}g/100mL$ at the end of 7th week), meaning iron could promote the mobilization of vitamin A from liver to serum. With the increase of dietary supplemental vitamin A levels, liver iron concentration decreased and serum iron concentration increased, vitamin A could promote the mobilization of iron from liver to blood. Iron concentration in Duodenum and tibia and erythrocyte count increased significantly with higher dietary vitamin A supplementation (p<0.01), vitamin A could promote iron absorption, iron mobilization from liver to target tissues and erythropoiesis. Effects of the interaction between iron and vitamin A on vitamin A concentrations in liver and serum, iron concentration in tibia and erythrocyte count were significant (p<0.05).

• Journal of Nutrition and Health
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• 제31권8호
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• pp.1254-1262
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• 1998

This study has done to investigate the relationship between the icreased lipid oncentration caused by smoking and plama levels of vitamin A and vitamin E, antiodative enzyme activity, and lipid peroxidation , in 52 male smokers and 32 non-smokers, Dietary vitamin A and vitamin E intake was imilar in both smokers and non-smokers. Absolute plasma concentrations of vitamin A and vitamin E were not significantly different between two groups, whereas vitamin E/cholesterol ration in plasma was low or in smokers than in that of non-smokers(p<0.05). It was considered that this lowered effect was due to the elevated plasma lipid concentration rather than oxidant stress derived from smoking, in view of the fact that smokers had higher cholesterol (15.2%) adn LDL-C(26.6%) levels than non-smokers. In non-smokers, plasma thiobarbiturin acid reactive substances(TBARS) conrrelated positively with total cholesterol(r=0.63466, p<0.001), LDL-C level(r=0.57166, p<0.01) , and LDL-C/HDL-C ratio(r=0.45926, p<0.05) . Activities of glutathione perosidase(GSH-Px) , superoside dismutase(SOD), and catalse made no difference in both groups. However, it was observed in non-smokers that GSH-Px activity had negative correlations with total cholesterol(r=-0.67293, p<0.001), LDL-C level(r=-0.62878, p<0.001), and LDL-C/HDL-C ratio (r=-0.58824, p<0.01), indicating that there was a dependent relationship between lipid perosidation and plasma lipid level. The smokers also showed negative correlations for GSH-Px activity with total cholesterol (r=-0.29946, p<0.05) and LDL-c level (r=0.45914, p<0.001), and LDL-C/HDL-c ratio(r=-0.35438, p<0.05). It seemed that the lipid that the lipid level elevated by sustaines smoking resulted in reducing vitamin E/cholesterol ratio and proportion of antioxidant to oxidant load, and then GSH-Px activity, with insufficient removal of free radicals(TBARS 2.43$\pm$0.51 and 1.81$\pm$0.15nmol/ml in smokers and non-smokers, respectively). These findings suggest that higher plasma lipid levels may play a more important role in perturbing the antioxidant defense system including vitamin E status and GSH-Px activity, at least in circumstances that increase lipid concentration . In addition, in exposure to free radicals like those in cigarette smoke. In those cases the ratio of vitamin E/lipid in plasma can be a more indicator of vitamin E status than plasma levels of vitamin E alone.