Yungmijihwgang-Won, Yollyunggobon-Dan and Palmi-Hwan, Korea traditional prescriptions composed of oriental medical herbs, have been used successfully to improve human health and regimen. This study was designed to examine the mechanism of healthful effects of the Korea traditional prescriptions through its antioxidative potentials. Using in vitro antioxidative activity assay system such as DPPH radical quenching assay, superoxide anion radical scavenging assay and inhibition of TBARS production, three Korea traditional prescriptions were observed to have nearly the same antioxidative potentials as ascorbic acid, a well-known strong water-soluble antioxidant. Moreover, we observed reinforced antioxidative effects of these drugs in liver from mouse fed these drugs with 4 weeks. When liver homogenate was incubated with 2.2'-azobis(amidinopropane) dihydrochloride(AAPH), as a free radical initiator, we observed that oxidative damages were decreased and antioxidative potentials were increased in liver homogenate treated these drugs. However, enzymatic antioxidative system as superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase was not affected by drug administration.
The biochemical components of Namcheondlggae, Miryangdlkkae 25, Boradlggae and Ipdlkkae 1 were measured. The samples were extracted with hot water, 60% acetone or 80% ethanol for screening physiological activity. The crude protein content (4.36%) was found in the Miryangdlkkae 25 and calcium content (497.5 mg%) was found in the Namcheondlggae among the tested 4 perilla loaves. Fructose was 30.86 mg% in the Namcheondlggae and free amino acids at all perilla leaves was detected seventeen. In Boradlggae, glutamic acid and alanine were 25.37 and 11.91 mg%. Totally nine non-volatile organic acids were also detected and the contents of malic acid and glutaric acid were 28.34 and 14.57 mg% in Boradlggae. The Miryangdlkkae 25 had the highest vitamin C amount which was 113.24 mg%. Angiotensin converting enzyme (ACE) inhibition activity of 60% acetone extract of Miryangdlkkae 25 was 39.20% when added as addition of 200 ppm level and xanthine oxidase inhibition activity of 80% ethanol extract of Boradlggae was 46.71%. Electron denoting activity of 60% acetone extract from Namcheohndlggae was the strongest inhibition activity as 98.19% when 200 ppm level of the sample extracts were added.
Kim, Myung-Ho;Lee, Won-Duck;Kim, Young-Ok;Kim, Moon-Shik
Journal of Preventive Medicine and Public Health
/
v.9
no.1
/
pp.95-108
/
1976
It is obvious that adequate nutrition is essential for growth and development of school children, and many elementary schools in this country have already practiced it. Therefore, it would seem apparent that the school feeding program would have a significant effect on the growth and development of school children. This paper presents a two-year experimental school-feeding program from 1973 to 1974, and attempts to evaluate its effects by before-and-after nutrition surveys conducted in two elementary schools, one experimental and the other as a control. The two schools are both located in the same county (Yongin-Kun, Kyunggi-Do), and the families of their students are presumed to share the same socio-economic level. To assess the effect of school-feeding, we measured height, weight, chest circumference and grasping power. Physical examination was done foresigns of nutritional deficiency. A stool examination for parasites and blood examinations for hemoglobin, hematocrit and serum protein were included. Analyses were done for 150 students selected randomly at the beginning of the program. These students attended the school throughout the program period. Results are as follows: 1. The amount of increase of height, weight, chest cirumference and grasping power were greater in the experimental school than in the control school, but the differences are not statistically significant. 2. Signs of vitamin deficiency decreased in both experimental and control schools during the two-year program period. 3. At the time of the 1974 post-survey, values of Hb. & Hct. revealed no significant differences between the two schools, but serum protein level was a little higher than that of general Korean rural children of the same age. 4. Infestation rate of parasites had increased in both schools during the two-year program period. 5. Each student of the two schools was classified into three major classes, according to the level of economic condition of his or her parents, namely higher, middle and lower. The results of each class of the experimental school was compared with that of the corresponding class of the control school, expecting the relative magnitude of change largest in the lower economic class of the experimental school. However, change was greatest in the middle class, still not being statistically significant. Finally, the authors concluded that the two-year period for such a program is not sufficiently long for its beneficial effects to be demonstrated and measured. As long as the growth and development of children are concerned, planning with a more distant perspective is required, as well as the development of new methods of evaluation.
Ju Yun-Sang;Jin Sun-Ja;Hwang Pil-Gi;Choi Chul-Ho;Lee Eui-Sang
KSBB Journal
/
v.19
no.6
s.89
/
pp.484-488
/
2004
Fermentation studies were conducted in batch and continuous flow cell-recycle reactors with food by-products as substrates. The genus Propionibacterium acidipropionici ATCC 4965 was utilized in the production of organic acids. Good performance was achieved in the batch fermentation using hydrol as a carbon source and corn steep liquor (CSL) as nitrogen and vitamin sources. Product yields and productivity based on maximum values were 0.80 g total acids/g glucose and 0.26 g total acids/L/h, respectively, when $3\%$, (w/v) of hydrol and $2.5\%$, (w/v) of CSL were utilized. Continuous fermentation with cell-recycling system using the optimum amounts of substrates resulted in dramatic increase in cell concentration (X) and maximum productivity (P). Compared to the batch fermentation, X and P were increased by as much as 21 and 13 times, respectively, at the dilution ratio of $0.2\;hr^{-1}$, indicating that cell recycling fermentation of food by-products provides valuable means for the mass production of organic acids as well as utilizing cell mass as good nutrient resources.
This study analyzed the quality characteristics of black garlic made from Seosan Yukjok Garlic and elephant garlic in Seosan, Chungnam province. Of the inorganic components, Mg content was the highest in all treatment groups, and the Ca content was high in each of the 15 day treatments. The content of K was high after 10 days aging in Yukjok garlic and after 15 days in the elephant garlic. The Fe, Na, K, and Mg content was high in Yukjok black garlic after 15 days, and Na, K, Ca, and Mg were high in the elephant black garlic aged for 15 days. The crude fat content was high in both Yukjok black garlic and elephant black garlic after 15 days. Vitamin C content was highest in both types of garlic after aging for 15 days. An analysis of four kinds of organic acids showed that citric acid was the only organic acid to appear in raw garlic of Yukjok garlic and elephant garlic. Black Yukjok garlic and elephant black garlic had a greater total amino acid content than the raw garlic of either type. However, among the tested amino acids, 13 kinds of amino acids were at their highest after five days of ripening in Yukjok black garlic, while 15 kinds of amino acids were abundant in elephant garlic after the same period. Eight kinds of amino acids were high after aging for 15 days. Through this study, it was confirmed that, in the process of making black garlic, changes in the main components of the garlic occur through different routes, and these changes vary depending on the garlic species. Therefore, this study provided basic data for the processing of Seosan's Yukjok black garlic and elephant black garlic.
Journal of the Korean Society of Food Science and Nutrition
/
v.21
no.2
/
pp.163-170
/
1992
Color of kimchi juice was measured instrumentally to evaluate the quality of Korean cabbage kimchi during fermentation at 4$^{\circ}C$ and 16$^{\circ}C$ . These results were compared with those of chemical analyses of kimchi juice and sensory evaluation for kimchi. Chemiral analyses and sensory evaluation showed that the kimchi has reached pH 4.3, the pH value under an optimum ripening period, after 3-days fermentation at 16$^{\circ}C$ with almost the highest contents of vitamin C and carotenoids as well as desirable sour taste and texture. At 4$^{\circ}C$ the kimchi was unripened after 6-days fermentation. At 16$^{\circ}C$, $L^{*}$ value of kimchi juice did not show any significant change during fermentation periods. However, $a^{*}$ value increased until day 4$^{\circ}C$ and thereafter decreased sharply. $b^{*}$ value increased until day 3 and then showed no change. High correlations were seen between volume of kimchi juice, pH, total acidity and $L^{*}$or $b^{*}$ value, and between carotenoid content and $a^{*}$ value, respectively. It was desirable to use color $a^{*}$ or $b^{*}$ value of kimchi juice to evaluate the quality of kimchi. The kimchi was under an over-ripening period when $a^{*}$ value was equal to or lower than the initial value, or when $b^{*}$ value was almost constant. Predicting an optimum ripening period was possible by using a proportional value of $a^{*/}$$b^{*}$ , i.e., unripening period, 1 $\geq$ and near 1 ; optimum ripening period, near 0.8 ; over-ripening period, < 0.8.eriod, < 0.8.d, < 0.8.
Kim, In-Deok;Kwon, Ryun-Hee;Heo, Ye-Young;Jung, Hye-Jin;Kang, Hwan-Yul;Ha, Bae-Jin
KSBB Journal
/
v.23
no.6
/
pp.529-534
/
2008
The reactive oxygen species generated by ultraviolet rays causes various types of cutaneous damage, such as lipid peroxidation and denaturation of the extra-cellular matrix. The accumulation of such damage contributes to skin aging, especially the formation of wrinkles. This study was carried out to develop functional cosmatic by using Oriental herb supercritical extracts (OHSE) for prevention of skin. Effects of OHSE on anti-oxidation, collagenase inhibition and collagen synthesis in normal human fibroblast were investigated. OHSE showed antioxidative activity as high as vitamin C, trolox and DL-penicillamine. Also OHSE showed promotive effect on collagen synthesis and inhibitory effect on collagenase activity. From this results, we conclude that OHSE may have the potential to be conveniently used as an additive in cosmetics for prevention and improvement of skin aging.
Ecological characteristics of microorganisms in tideland soils were studied by investigation of microbial diversity and population. Twenty soil samples were taken at surface, 10, 20 and 30 cm depth each. Bacteria, actinomycetes and fungi were isolated on each selective isolation medium containing different concentration of NaCl. Actinomycetes were the most isolated from soil samples taken at 10 cm depth and isolated by humic acid-vitamin (HV) medium without sea water or salt. Twenty nine strains of actinomycetes were isolated at surface soil and 74, 39, 37 strains were at 10, 20, and 30 cm depth, respectively. All these isolates were analysed and grouped by random amplified polymorphic DNA (RAPD)-PCR analysis. Many of the isolates were clustered into Microtetraspora and Pseudonocardia. Fungal isolates were highly distributed at the surface soil and isolated well on potato dextrose agar (PDA) medium with sea water. Bacterial isolates were higly distributed at surface soil and isolated well by nutrient medium without sea water or salt. Soil samples taken at 10 cm depth showed the highest microbial diversity and population.
Kim, Hyun-Jung;Lee, Jung-Jin;Cheigh, Mee-Jung;Choi, Shin-Yang
Korean Journal of Food Science and Technology
/
v.30
no.6
/
pp.1333-1338
/
1998
Several enzymes of kimchi ingredients were assayed to improve the product quality using these quality related enzyme information. Among various hydrolases, amylase and protease were selected with respect to lactic acid fermentation. Peroxidase and ascorbic acid oxidase were studied for off flavor production and ascorbic acid destruction. The amount of protein in kimchi ingredients, specific and total enzyme activity of sample were compared. Regarding total enzyme activity of sample, ${\alpha}-amylase$ activity of salted and fermented anchovy, dried red pepper and salted and fermented shrimp were higher than other ingredients. Activity of salted and fermented anchovy was 2,790.0 units/g sample. Salted and fermented anchovy, oyster and Chinese radish showed the highest ${\beta}-amylase$ activity (4.4, 2.1, 1.0 units/g sample, respectively). Salted and fermented anchovy showed the highest protease activity of 13.4 PU/g sample, followed by salted and fermented shrimp and dried red pepper. For peroxidase, Chinese radish, cucumber, green onion showed the highest activity of 7.2, 6.8 and 5.6 units/g sample, respectively. In case of ascorbic acid oxidase, salted and fermented anchovy showed the strongest enzyme activity (331.4 units/g sample), followed by dried red pepper and salted and fermented shrimp.
This study investigated intake patterns of health functional foods(HFF) in elementary school children and requirements of their parents in the Daejeon area using a questionnaire by 432 parents. Participating parents were composed of female, 83.6% and male, 16.4%. Most of the parents were in their 40's(53.0%) and 30's(45.8%). 47.9% of the children were male and 52.1% were female. The school year of the children was distributed evenly, ranging from the first year to the sixth year. The intake rate of HFF for parents appeared to be 65.3% and for children it appeared to be 63.2%. The primary reason of using HFF to their child was 'For health promotion'(54.4%); the largest reason of non-using HFF was 'Do not feel necessity because is healthy'(48.8%). In regards to purchase place, 'Drugstore' was most selected by 26.1%, while the largest amount of purchase motive was 'Decided by oneself for children's health'(37.7%). The average expense per month was, 'Less than 50 thousand portion'(20.3%). The intake period, 'less than 6 months' was won' for 54.9%. For intake items, 'Vitamin supplementation products' was the largest portion'(20.3%) was indicated. The intake period, less than 6 months' was the most selected by 51.3%. Most people(66.7%) selected '1 kind' of intake item. As for intake effect, 45.4% claimed 'A little help'. In regard to experiencing side effects, 'Is not' was 92.7%, however, diarrhea, nettle rash, nausea etc. were a little. The biggest problem of HFF was 'Falsehood/exaggerative advertisement' as identified by 53.1%. For improvement of the HFF system was, 'Verify by more strict formality' as selected by 55.8%. In regards to experience of nutrition education on HFF, 'Is not' was most selected by 51.0%. The most desirous form of nutrition education was, 'Simple paper material'(31.3%). The most desirous content of nutrition education was, 'nutritional management of classified by life cycle'(37.2%). Therefore, HFF must be used properly to promote the health and growth development of children by acquiring scientific and reasonable information about the ability and usage of the food.
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