• 제목/요약/키워드: violet

검색결과 958건 처리시간 0.025초

Enterobacter cloacae MG82에 의한 Crystal Violet의 탈색특성 (Decolorizing Characteristics of Crystal Violet by Enterobacter cloace MG82.)

  • 정민선;지원대;김병홍;정영건
    • 한국미생물·생명공학회지
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    • 제26권3호
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    • pp.269-274
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    • 1998
  • Triphenylmethane계 염료 탈색균인 E. cloacae MG 82를 이용한 crystal violet의 탈색 특성을 조사하였다. 배지내의 용존산소의 양이 많을수록 crystal violet의 탈색과 E. cloacae MG82의 균생장이 좋았다. 균생장 중기의 세포 군에서 crystal violet의 탈색능이 가장 활발하였고 염료의 농도가 높아짐에 따라 균생장과 탈색능이 억제되었으며, 균생장이 가능한 crystal violet의 최고 농도치는 375 $\mu$M이었다. E. cloacae MG82는 crystal violet을 유일한 탄소원으로 이용할 수 없었고, 균생장 및 염료의 탈색에 또 다른 에너지원을 필요로 하였다.

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Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells

  • Zhang, Xiao-Hong;Zhang, Nan;Lu, Jian-Mei;Kong, Qing-Zhong;Zhao, Yun-Feng
    • Biomolecules & Therapeutics
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    • 제20권2호
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    • pp.177-182
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    • 2012
  • Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the first time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The results showed that tetrazolium violet significantly decreased the viability of A549 cells at $5-15{\mu}M$. Tetrazolium violet -induced apoptosis in A549 cells was confirmed by H33258 staining assay. In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21/WAF1 expression. In addition, an enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as caspase, were responsible for the apoptotic effect induced by tetrazolium violet. The conclusion of this study is that tetrazolium violet induced p53 expression which caused cell cycle arrest and apoptosis. These findings suggest that tetrazolium violet has strong potential for development as an agent for treatment lung cancer.

건축물 외장재 열화현상에 영향을 미치는 자외선 발생량 추정 및 기후 맵 작성 (Assumption and Climate-Map Drawing of Ultra Violet Rays Influencing to the Deterioration of Building Exterior Materials)

  • 이정재
    • KIEAE Journal
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    • 제6권2호
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    • pp.19-26
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    • 2006
  • Recently, building requirements have been saturated and residents are demanding a variety of building exterior materials faced with roofs, walls, windows and doors. On the other hand, when building exterior materials are exposed with ultra violet rays during some periods, these will be influenced deterioration effects like discolorations, exfoliations and cracks and will be needed exchange of materials. In this study, main 17-provinces of Korea were collated climate data and counted the computation results with an assumption formula for calculating ultra violet rays amounts. Lastly, ultra violet rays map of Korea was made by the counted data and was considered tendencies. Accordingly, in the phase of design the designer can select the appropriate exterior materials which have durabilities and resistances of ultra violet rays. So hereafter this study can be a foundation study to sustain building exterior materials with ultra violet rays damages. Below is the summary of Korean ultra violet rays tendency. 1. Ultra violet rays during 1 year is from about $180MJ/m^2$ to about $210MJ/m^2$. 2. Southern province ultra violet rays is higher than northern province. 3. Annually counted rays is higher and higher.

Production of Antibacterial Violet Pigment by Psychrotropic Bacterium RT102 Strain

  • Nakamura, Yoshitoshi;Asada, Chikako;Sawada, Tatsuro
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제8권1호
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    • pp.37-40
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    • 2003
  • The antibacterial action of violet pigment, a mixture of violacein and deoxyviolacein, isolated from phychrotrophic bacterium RT102 strain was examined, and the operational conditions for the effective production of violet pigment were studied. The antibacterial activity of the violet pigment was confirmed for several bacteria such as Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, and Pseudomonas aeruginosa, and the high concentration of violet pigment, above about 15mg/L, caused not only growth inhibition but also death of cells. The growth properties of RT102 strain were clarified under various incubation conditions such as pH, temperature, and dissolved oxygen concentration. The maximum violet pigment concentration, i.e. 3.7 g/L, and the maximum productivity of violet pigment, i.e. 0.12 g .L$\^$-1/H$\^$-1/, were obtained in a batch culture of pH 6, 20$^{\circ}C$, and 1 mg/L of dissolved oxygen concentration.

보라색의 유래 및 이미지의 고찰 (A study on the origin and the images of violet)

  • 김은경;김영인
    • 디자인학연구
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    • 16호
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    • pp.225-234
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    • 1996
  • 본 연구는 최초의 합성염료인 보라색을 중심으로 보라색의 유래 및 이미지를 고찰하고 그 이미지의 특성을 규명함으로서, 디자인에 활용할 수 있는 보라색의 종합적인 색채 자료를 제시하는 데 있다. 본 연구의 결과는 다음과 같다. 1) 색 이름의 특성에 의한 고찰 결과, 보라색은 퍼플, 바이올렛, 모브, 마젠타 순의 색 명으로 사용되어왔으며, 어두운 붉은 보라계열에서 합성염료의 개발에 의한 밝은 보라계열까지로 확대되어 온 것으로 나타났다. 2)보라색은 색채 표현적 특성상 심리적, 상징적, 감성적으로 양극단의 성격을 지닌 빨강과 파랑이 혼합되어 양면적인 속성을 지닌 중성 색으로 나타났다. 이에 따른 긍정적인 이미지는 고귀한·신성한·신비한·수면·진정·관능적·여성적, 향기로운 이미지로 분류될 수 있었으며, 부정적인 이미지는 연약함·슬픔·우울, 죽음(왕가의 상복), 타락한 힘의 상징, 미신 등의 이미지로 분류될 수 있었다.

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자경종과 황숙종인삼의 $F_1$$F_2$ 세대의 형질특성과 경색분리 (Characters and Inheritance of Stem Color in $F_1$ and $F_2$ of Violet-Stem Variant x Yellow-Berry Variant in Panax ginseng C. A. Meyer)

  • 천성룡;안상득
    • Journal of Ginseng Research
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    • 제9권2호
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    • pp.264-269
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    • 1985
  • The inheritance of violet and green stems was examined in F1 F2 of violetstem variant x yellow-berry variant in Panax ginseng C. A. Meyer, and the characters of F1 and F2 plants were investigated. From these results, it was shown that most of the characters of F1 and F2 plants were simillar to the female plants. However, reciprocal crosses between violet and green stems yielded progeny of violet-stem variant. Thus the cross V♀x♂Y gave all violet-stem seedlings, and the cross Y♂ x Y♀ gave all violet-stem seedlings. And all of the crosses segregated in F2 in a ratio of 3 violet to 1 green.

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Citrobacter sp.에 의한 Triphenylmethane계 색소의 분해 (Degradation oof Triphenylmrthane Dyes by Citobacter sp.)

  • 민상기;조영배;전홍기
    • 생명과학회지
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    • 제5권1호
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    • pp.8-19
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    • 1995
  • The Optimal condition for degradation of crystal violet and other triphenylmethane dyes by Citrobacter sp. SK-3 isolated from the activated sludge of dye manufacturing factory was investigated. The optimal culture medium for the degradation of triphenylmethane dye was composed of minimum inorganic salt medium supplemented with 0.5% galactose, 0.1% beef extract, with the initial pH of 8.0 to 9.0. Under this condition, Citrobacter sp. SK-3 degraded 200 ppm of crystal violet completely within 24 hours. Citrobactre sp. SK-3 also degraded efficiently malachite green, pararosaniline, brilliant green, methyl violet, basic fuchsin and methyl red. Analysis of the degradation products of crystal violet through this layer chromatography and high performance liquid chromatography indicated that the methyl groups bound to crystal violet backborn were gradually demethylated to pentamethyl-, tetramethyl- and trimethylpararosaniline.

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활성탄 흡착에 의한 Ethyl Violet의 공정 파라미터 연구 (Study on Process Parameter of Ethyl Violet by Activated Carbon Adsorption)

  • 이종집
    • 한국수처리학회지
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    • 제26권6호
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    • pp.143-152
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    • 2018
  • The process parameters of ethyl violet from aqueous solution by activated carbon adsorption were carried out as a function of pH, temperature, contact time, initial concentration and temperature. The adsorption equilibrium data can be described well by the Langmuir and Freundlich isotherm models. Base on Langmuir constant ($R_L=0.0343{\sim}0.0523$) and Freundlich constant (1/n=0.1633~0.1974), This process could be employed as effective treatment for adsorption of ethyl violet. The kinetic experimental results showed that the adsorption process can be well described with the pseudo second order model. Based on the positive enthalpy (6.505 kJ/mol), the adsorption of ethyl violet onto granular activated carbon is endothermic. The negative Gibbs free energy (-1.169~-1.681 kJ/mol) obtained indicates that the adsorption process is spontaneous and physisorption.

Aggregation of Crystal Violet with Tetraphenylborate Anions in Aqueous Solutions

  • Lee, Beom-Gyu;Jung, Rae-Seok;Kim, Kang-Jin
    • Bulletin of the Korean Chemical Society
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    • 제10권2호
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    • pp.148-151
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    • 1989
  • The hydrophobic interaction between tetraphenylborate (TPB$^-$) or tetrakis (4-fluorophenyl)borate (TFB$^-$) and crystal violet has been investigated in aqueous solutions by absorption spectrophotometry. Both of the anions promote the aggregation of the ion pairs formed between crystal violet and TPB$^-$ or TFB$^-$. When crystal violet and borate anion are nearly equimolar, insoluble floating aggregates can be observed. Based on the relative absorbance of H and J bands and on the effect of TX-100, TFB$^-$ is found to be more hydrophobic than TPB$^-$.

Decolorization and Biotransformation of Triphenylmethane Dye, Methyl Violet, by Aspergillus sp. Isolated from Ladakh, India

  • Kumar, C. Ganesh;Mongolla, Poornima;Basha, Anver;Joseph, Joveeta;Sarma, V.U.M.;Kamal, Ahmed
    • Journal of Microbiology and Biotechnology
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    • 제21권3호
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    • pp.267-273
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    • 2011
  • Methyl violet, used extensively in the commercial textile industry and as a biological stain, is a hazardous recalcitrant. Aspergillus sp. strain CB-TKL-1 isolated from a water sample from Tsumoriri Lake, Karzok, Ladakh, India, was found to completely decolorize methyl violet within 24 h when cultured under aerobic conditions at $25^{\circ}C$. The rate of decolorization was determined by monitoring the decrease in the absorbance maxima of the dye by UV-visible spectroscopy. The decolorization of methyl violet was optimal at pH 5.5 and $30^{\circ}C$ when agitated at 200 rpm. Addition of glucose or arabinose (2%) as a carbon source and sodium nitrate or soyapeptone (0.2%) as a nitrogen source enhanced the decolorization ability of the culture. Furthermore, the culture exhibited a maximum decolorization rate of methyl violet after 24 h when the C:N ratio was 10. Nine N-demethylated decolorized products of methyl violet were identified based on UV-visible spectroscopy, Fourier transform infrared (FTIR), and LC-MS analyses. The decolorization of methyl violet at the end of 24 h generated mono-, di-, tri-, tetra-, penta-, and hexa-N-demethylated intermediates of pararosaniline. The variation of the relative absorption peaks in the decolorized sample indicated a linear decrease of hexa-N-demethylated compounds to non-N-demethylated pararosaniline, indicating a stepwise N-demethylation in the decolorization process.