• Title/Summary/Keyword: violet

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Decolorizing Characteristics of Crystal Violet by Enterobacter cloace MG82. (Enterobacter cloacae MG82에 의한 Crystal Violet의 탈색특성)

  • 정민선;지원대;김병홍;정영건
    • Microbiology and Biotechnology Letters
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    • v.26 no.3
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    • pp.269-274
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    • 1998
  • Decolorizing characteristics of crystal violet by Enterobacter cloace MG82, which can decolorize rapidly triphenylmethane dyes, were investigated. The higher growth and decolorization activity was shown at big ratio of dissolved oxygen in the medium. The decolorization activity of crystal violet revealed highest at the middle of lag phase. As the concentration of crystal violet was higher, the growth of E. cloacae MG82 and decolorizing activity of crystal violet by this strain were worse. The maximum concentration of crystal violet at which E. cloacae MG82 be able to grow was 375 ${\mu}$M. E. cloacae MG82 was not able to use the crystal violet itself as a sole carbon source. So, it was shown that growth of E. cloacae MG82 and decolorization activity of crystal violet by this strain needed addition of another energy sources except this dye.

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Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells

  • Zhang, Xiao-Hong;Zhang, Nan;Lu, Jian-Mei;Kong, Qing-Zhong;Zhao, Yun-Feng
    • Biomolecules & Therapeutics
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    • v.20 no.2
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    • pp.177-182
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    • 2012
  • Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the first time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The results showed that tetrazolium violet significantly decreased the viability of A549 cells at $5-15{\mu}M$. Tetrazolium violet -induced apoptosis in A549 cells was confirmed by H33258 staining assay. In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21/WAF1 expression. In addition, an enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as caspase, were responsible for the apoptotic effect induced by tetrazolium violet. The conclusion of this study is that tetrazolium violet induced p53 expression which caused cell cycle arrest and apoptosis. These findings suggest that tetrazolium violet has strong potential for development as an agent for treatment lung cancer.

Assumption and Climate-Map Drawing of Ultra Violet Rays Influencing to the Deterioration of Building Exterior Materials (건축물 외장재 열화현상에 영향을 미치는 자외선 발생량 추정 및 기후 맵 작성)

  • Yee, Jurng-Jae
    • KIEAE Journal
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    • v.6 no.2
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    • pp.19-26
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    • 2006
  • Recently, building requirements have been saturated and residents are demanding a variety of building exterior materials faced with roofs, walls, windows and doors. On the other hand, when building exterior materials are exposed with ultra violet rays during some periods, these will be influenced deterioration effects like discolorations, exfoliations and cracks and will be needed exchange of materials. In this study, main 17-provinces of Korea were collated climate data and counted the computation results with an assumption formula for calculating ultra violet rays amounts. Lastly, ultra violet rays map of Korea was made by the counted data and was considered tendencies. Accordingly, in the phase of design the designer can select the appropriate exterior materials which have durabilities and resistances of ultra violet rays. So hereafter this study can be a foundation study to sustain building exterior materials with ultra violet rays damages. Below is the summary of Korean ultra violet rays tendency. 1. Ultra violet rays during 1 year is from about $180MJ/m^2$ to about $210MJ/m^2$. 2. Southern province ultra violet rays is higher than northern province. 3. Annually counted rays is higher and higher.

Production of Antibacterial Violet Pigment by Psychrotropic Bacterium RT102 Strain

  • Nakamura, Yoshitoshi;Asada, Chikako;Sawada, Tatsuro
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.1
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    • pp.37-40
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    • 2003
  • The antibacterial action of violet pigment, a mixture of violacein and deoxyviolacein, isolated from phychrotrophic bacterium RT102 strain was examined, and the operational conditions for the effective production of violet pigment were studied. The antibacterial activity of the violet pigment was confirmed for several bacteria such as Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, and Pseudomonas aeruginosa, and the high concentration of violet pigment, above about 15mg/L, caused not only growth inhibition but also death of cells. The growth properties of RT102 strain were clarified under various incubation conditions such as pH, temperature, and dissolved oxygen concentration. The maximum violet pigment concentration, i.e. 3.7 g/L, and the maximum productivity of violet pigment, i.e. 0.12 g .L$\^$-1/H$\^$-1/, were obtained in a batch culture of pH 6, 20$^{\circ}C$, and 1 mg/L of dissolved oxygen concentration.

A study on the origin and the images of violet (보라색의 유래 및 이미지의 고찰)

  • 김은경;김영인
    • Archives of design research
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    • no.16
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    • pp.225-234
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    • 1996
  • The purpose of this study is to provide and suggest useful data in use of kinds of violet that is resulted from general color images of violet. The origin, history, and general images of kinds of violet were studied, and were classified vased on documcntary reviews. Findings are as follows: 1) The kinds of violet were used purple, violet, mauva, and magenta in the order of appearance of its color names and the categories of violet had been extended from dark reddish violet to affilicated colors of bright violet by development in synthetie dyestuff. 2) The kinds of violet has neutral characters due to the combination of two extreme colors, red and blue, and thereby revealing voth attreibutes in terms of symbolic, emotional, and spychological features of colors. The positive images in kinds of violet can be classified as nobleness, holiness, mysteriousness, sleep/sedation, sensuality, femininity, fragrance, and the negatibely classified images are weakness, sadness, maelancholiness, death(for example, the mourner's gard in royal families), symbols of moral corruption, and superstition.

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Characters and Inheritance of Stem Color in $F_1$ and $F_2$ of Violet-Stem Variant x Yellow-Berry Variant in Panax ginseng C. A. Meyer (자경종과 황숙종인삼의 $F_1$$F_2$ 세대의 형질특성과 경색분리)

  • 천성룡;안상득
    • Journal of Ginseng Research
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    • v.9 no.2
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    • pp.264-269
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    • 1985
  • The inheritance of violet and green stems was examined in F1 F2 of violetstem variant x yellow-berry variant in Panax ginseng C. A. Meyer, and the characters of F1 and F2 plants were investigated. From these results, it was shown that most of the characters of F1 and F2 plants were simillar to the female plants. However, reciprocal crosses between violet and green stems yielded progeny of violet-stem variant. Thus the cross V♀x♂Y gave all violet-stem seedlings, and the cross Y♂ x Y♀ gave all violet-stem seedlings. And all of the crosses segregated in F2 in a ratio of 3 violet to 1 green.

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Degradation oof Triphenylmrthane Dyes by Citobacter sp. (Citrobacter sp.에 의한 Triphenylmethane계 색소의 분해)

  • 민상기;조영배;전홍기
    • Journal of Life Science
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    • v.5 no.1
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    • pp.8-19
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    • 1995
  • The Optimal condition for degradation of crystal violet and other triphenylmethane dyes by Citrobacter sp. SK-3 isolated from the activated sludge of dye manufacturing factory was investigated. The optimal culture medium for the degradation of triphenylmethane dye was composed of minimum inorganic salt medium supplemented with 0.5% galactose, 0.1% beef extract, with the initial pH of 8.0 to 9.0. Under this condition, Citrobacter sp. SK-3 degraded 200 ppm of crystal violet completely within 24 hours. Citrobactre sp. SK-3 also degraded efficiently malachite green, pararosaniline, brilliant green, methyl violet, basic fuchsin and methyl red. Analysis of the degradation products of crystal violet through this layer chromatography and high performance liquid chromatography indicated that the methyl groups bound to crystal violet backborn were gradually demethylated to pentamethyl-, tetramethyl- and trimethylpararosaniline.

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Study on Process Parameter of Ethyl Violet by Activated Carbon Adsorption (활성탄 흡착에 의한 Ethyl Violet의 공정 파라미터 연구)

  • Lee, Jong-Jib
    • Journal of Korean Society of Water Science and Technology
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    • v.26 no.6
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    • pp.143-152
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    • 2018
  • The process parameters of ethyl violet from aqueous solution by activated carbon adsorption were carried out as a function of pH, temperature, contact time, initial concentration and temperature. The adsorption equilibrium data can be described well by the Langmuir and Freundlich isotherm models. Base on Langmuir constant ($R_L=0.0343{\sim}0.0523$) and Freundlich constant (1/n=0.1633~0.1974), This process could be employed as effective treatment for adsorption of ethyl violet. The kinetic experimental results showed that the adsorption process can be well described with the pseudo second order model. Based on the positive enthalpy (6.505 kJ/mol), the adsorption of ethyl violet onto granular activated carbon is endothermic. The negative Gibbs free energy (-1.169~-1.681 kJ/mol) obtained indicates that the adsorption process is spontaneous and physisorption.

Aggregation of Crystal Violet with Tetraphenylborate Anions in Aqueous Solutions

  • Lee, Beom-Gyu;Jung, Rae-Seok;Kim, Kang-Jin
    • Bulletin of the Korean Chemical Society
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    • v.10 no.2
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    • pp.148-151
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    • 1989
  • The hydrophobic interaction between tetraphenylborate (TPB$^-$) or tetrakis (4-fluorophenyl)borate (TFB$^-$) and crystal violet has been investigated in aqueous solutions by absorption spectrophotometry. Both of the anions promote the aggregation of the ion pairs formed between crystal violet and TPB$^-$ or TFB$^-$. When crystal violet and borate anion are nearly equimolar, insoluble floating aggregates can be observed. Based on the relative absorbance of H and J bands and on the effect of TX-100, TFB$^-$ is found to be more hydrophobic than TPB$^-$.

Decolorization and Biotransformation of Triphenylmethane Dye, Methyl Violet, by Aspergillus sp. Isolated from Ladakh, India

  • Kumar, C. Ganesh;Mongolla, Poornima;Basha, Anver;Joseph, Joveeta;Sarma, V.U.M.;Kamal, Ahmed
    • Journal of Microbiology and Biotechnology
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    • v.21 no.3
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    • pp.267-273
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    • 2011
  • Methyl violet, used extensively in the commercial textile industry and as a biological stain, is a hazardous recalcitrant. Aspergillus sp. strain CB-TKL-1 isolated from a water sample from Tsumoriri Lake, Karzok, Ladakh, India, was found to completely decolorize methyl violet within 24 h when cultured under aerobic conditions at $25^{\circ}C$. The rate of decolorization was determined by monitoring the decrease in the absorbance maxima of the dye by UV-visible spectroscopy. The decolorization of methyl violet was optimal at pH 5.5 and $30^{\circ}C$ when agitated at 200 rpm. Addition of glucose or arabinose (2%) as a carbon source and sodium nitrate or soyapeptone (0.2%) as a nitrogen source enhanced the decolorization ability of the culture. Furthermore, the culture exhibited a maximum decolorization rate of methyl violet after 24 h when the C:N ratio was 10. Nine N-demethylated decolorized products of methyl violet were identified based on UV-visible spectroscopy, Fourier transform infrared (FTIR), and LC-MS analyses. The decolorization of methyl violet at the end of 24 h generated mono-, di-, tri-, tetra-, penta-, and hexa-N-demethylated intermediates of pararosaniline. The variation of the relative absorption peaks in the decolorized sample indicated a linear decrease of hexa-N-demethylated compounds to non-N-demethylated pararosaniline, indicating a stepwise N-demethylation in the decolorization process.