• Archives of Plastic Surgery
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• 제34권1호
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• pp.1-7
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• 2007

Purpose: Human adipose tissue-derived stromal cells(hADSCs) can be expanded in vitro and induced to differentiate into multiple mesenchymal cell types. In this study we have examined various neuronal phenotypes and gene expression profiles of the hADSCs in the neuronal induction. Methods: The hADSCs were isolated from human adipose tissue and they were characterized by the flow cytometry analysis using CD13, CD29, CD34, CD45, CD49d, CD90, CD105 and HLA-DR cell surface markers. We differentiated the hADSCs into the neuronal lineage by using chemical induction medium and observed the cells with contrast microscopy. The immunocytochemistry and western blotting were performed using the NSE, NeuN, Trk-A, Vimentin, N-CAM, S-100 and ${\beta}$-Tubulin III antibodies. Results: The hADSCs were positive for CD13($90.3{\pm}4%$), CD29($98.9{\pm}0.7%$), CD49d($13.6{\pm}6%$), CD90 ($99.4{\pm}0.1%$), CD105($96%{\pm}2.8%$) but negative for CD34, CD45 and HLA-DR. The untreated cultures of hADSCs predominately consisted of spindle shaped cells and a few large, flat cells. Three hours after the addition of induction medium, the hADSCs had changed morphology and adopted neuronal-like phenotypes. The result of immunocytochemistry and western blotting showed that NSE, NeuN, Trk-A, Vimentin, N-CAM, S-100 and ${\beta}$-Tubulin III were expressed. However, NSE, NeuN, Vimentin were weakly expressed in the control. Conclusion: Theses results indicate that hADSCs have the capabillity of differentiating into neuronal lineage in a specialized culture medium. hADSCs may be useful in the treatment of a wide variety of neurological disorders.

• Journal of Chest Surgery
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• 제26권12호
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• pp.962-964
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• 1993

Myoepithelioma of the trachea is an extremely rare disease. The neoplasm shows histologic features identical to those described in myoepithelioma of salivary glands. The myoepithelioma cells demonstrate the numerous myofilament, desmosomal cellular attachment, cytoplasmic glycogen and pinocytotic vesicles. In immunohistochemical study, myoepithelioma cells show the positive antibody reactions to actin, keratin, vimentin and S-100 protein. A case of myoepithelioma arising from trachea in the seventy-two-year old male diagnosed by microscopic finding and immunohistochemistry is presented.

• Asian-Australasian Journal of Animal Sciences
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• 제31권3호
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• pp.439-448
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• 2018

Objective: Myogenic satellite cells were isolated from semitendinosus muscle of prepubertal Korean black goat to observe the differential effect of linolenic and retinoic acid in thepresence of thiazolidinediones (TZD) and also to observe the production insulin sensitive preadipocyte. Methods: Cells were characterized for their stemness with cluster of differentiation 34 (CD34), CD13, CD106, CD44, Vimentin surface markers using flow cytometry. Cells characterized themselves as possessing significant (p<0.05) levels of CD13, CD34, CD106, Vimentin revealing their stemness potential. Goat myogenic satellite cells also exhibited CD44, indicating that they possessed a % of stemness factors of adipose lineage apart from their inherent stemness of paxillin factors 3/7. Results: Cells during proliferation stayed absolutely and firmly within the myogenic fate without any external cues and continued to show a significant (p<0.05) fusion index % to express myogenic differentiation, myosin heavy chain, and smooth muscle actin in 2% horse serum. However, confluent myogenic satellite cells were the ones easily turning into adipogenic lineage. Intriguingly, upregulation in adipose specific genetic markers such as peroxisome proliferation-activated receptor ${\gamma}$, adiponectin, lipoprotein lipase, and CCAAT/enhancer binding protein ${\alpha}$ were observed and confirmed in all given treatments. However, the amount of adipogenesis was found to be statistically significant (p<0.01) with linolenic acid as compared to retinoic acid in combination with TZD's. Conclusion: Retinoic acid was found to produce smaller preadipocytes which have been assumed to have insulin sensitization and hence retinoic acid could be used as a potential agent to sensitize tissues to insulin in combination with TZD's to treat diabetic conditions in humans and animals in future.

• 대한골관절종양학회지
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• 제14권1호
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• pp.68-72
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• 2008

유척색종은 전세계적으로 소수의 증례만이 보고될 정도로 매우 드문 종양으로서 척색종과 조직학적으로 매우 유사한 소견을 보이지만 구간 골격에 위치하는 척색종과 달리 유척색종은 구간외 골격 조직에 발생한다. 또한 국소적으로 재발하는 것으로 알려져 있다. 저자들은 2개월 전 서혜부 연부 조직 종양으로 국소 마취하에 단순 절제술을 시행받은 후 재발하여 광범위 절제술을 재시행하였던 유척색종 증례를 경험하였기에 문헌 고찰과 함께 보고하고자 한다. 척색종의 전이를 배제하기 위하여 골주사 검사를 시행하여 골병변이 없음을 확인하였고, 절제된 조직의 종양 세포는 면역조직화학 염색상 Epithelial membrane antigen(EMA), S-100 단백질, vimentin, cytokeratin에 양성반응을 보였다.

• 대한한방내과학회지
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• 제33권1호
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• pp.83-101
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• 2012

Objectives : This study was aimed to investigate the therapeutic effect of Bogijetongtanggammi-bang (BJTG) on injury of the peripheral nerve tissues. Methods : Rats were divided into 2 groups. The rats of the first group were injected with Taxol (1.25 mg/kg) to their sciatic nerves, once each. The sciatic nerves of the rats of the second group were crushed by forcept for 30 seconds. Rats were administered with BJTG (400 mg/kg) or 0.9% saline for 5 days. Changes of DRG neurons, Schwann cells, Cdc2, caspase 3. phospho-p44/42 Erk1/2, phospho-vimentin and ${\beta}1$ integrin were observed by fluorescent microscope and analysed in western blot. Results : In Taxol-treated SD rat models, BJTG up-regulated neurite outgrowth, Schwann cells, Cdc2 and phospho-Erk1/2, and down-regulated caspase 3. In pressure-injured rat models, BJTG up-regulated axons of sciatic nerve, Schwann cells, Cdc2, phospho-vimentin, ${\beta}1$ integrin, and down-regulated caspase 3. Conclusions : Taken together, BJTG was promotive of nerve regeneration on SNI as well as Taxol-induced nerve injury. BJTG had a pharmaceutical property enhancing recovery of injured peripheral nerves and could be a candidate for drug development after further research.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1187-1192
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• 2014

Background: To correlate breast cancer subtypes with prognostic factors, microvessel density (MVD), vascular endothelial growth factor (VEGF) expression and clinical features. Materials and Methods: One hundred cases of primary breast carcinoma were classified using biomarkers on tissue microarray as: luminal A [estrogen receptor (ER)+, HER2-, $Ki-67{\leq}14%$], luminal B [ER+, HER2+ or ER+, HER2-, Ki-67>14%], HER2, triple negative basal-like (TNB) [any basal cytokeratins (CKs, 5, 14, 17) and/or endothelial growth factor receptor (EGFR) expression], and TN without such markers [TNN, null], and assessed for p53, vimentin, VEGF and CD31 immunoperoxidase. Results: Of the 100 cases (mean age, 51 years; mean tumor size, 3.2cm; 56% with nodal metastasis; 89 invasive ductal carcinomas, not otherwise specified, 4 invasive lobular carcinomas, 3 metaplastic carcinomas, and 4 other types) there were 39 luminal A, 18 luminal B, 18 HER2, 15 TNB and 10 TNN. The positivities of basal-like markers in the basal-like subtype were 78.3% for CK5, 40% for CK14, 20% for CK17, 46.7% for EGFR. There was no significant difference in age distribution, tumor size, degree of tubular formation, pleomorphism, lymphovascular invasion, nodal metastasis, MVD, VEGF expression and survival among subgroups. TNs demonstrated significantly higher tumor grade, mitotic count, Ki-67 index, p53 and vimentin and decreased overall survival compared with nonTN. Conclusions: The distribution of breast cancer subtypes in this study was similar to other Asian countries with a high prevalence of TN. The high grade character of TN was confirmed and CK5 expression was found to be common in our basal-like subtype. No significant elevation of MVD or VEGF expression was apparent.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2777-2783
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• 2015

Background: Nestin is associated with neoplastic transformation. However, the mechanisms by which nestin contributes regarding invasion and malignancy of gastric adenocarcinoma (GAC) remain unknown. Recent studies have shown that the epithelial-mesenchymal transition (EMT) is important in invasion and migration of cancer cells. In the present study, we aimed to investigate the expression of nestin and its correlation with EMT-related proteins in GAC. Materials and Methods: The expression of nestin and EMT-related proteins was examined in GAC specimens and cell lines by immunohistochemistry and Western blotting. Clinicopathological features and survival outcomes were retrospectively analyzed. Results: Positive nestin immunostaining was most obviously detected in the cytoplasm, nucleus or both cytoplasm and nucleus of tumor cells in 19.2% (24/125) of GAC tissues, which was significantly higher than that in normal gastric mucosa tissues (1.7%, 1/60) (p=0.001). Nestin expression was closely related to several clinicopathological factors and EMT-related proteins (E-cadherin, vimentin and Snail) and displayed a poor prognosis. Interestingly, simultaneous cytoplasmic and nuclear nestin expression correlated with EMT-related proteins (E-cadherin, vimentin and Snail) (p<0.05) and lymph node metastasis (p=0.041) and a shorter survival time (p<0.05), but this was not the case with cytoplasmic or nuclear nestin expression. Conclusions: Nestin, particularly expression in both cytoplasm and nucleus, might be involved in regulating EMT and malignant progression in GAC, with potential as an unfavorable indicator in tumor diagnosis and a target for clinical therapy.

• Asian Pacific Journal of Cancer Prevention
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• 제15권10호
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• pp.4271-4274
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• 2014

The epithelial to mesenchymal transition (EMT) is a key step during embryonic morphogenesis and plays an important role in drug resistance and metastasis in diverse solid tumors. We previously reported that 48 h treatment of anti-cancer drug doxorubicin could induce EMT in human gastric cancer BGC-823 cells. However, the long term effects of this transient drug treatment were unknown. In this study we found that after 48 h treatment with $0.1{\mu}g/ml$ doxorubicin, most cells died during next week, while a minor population of cells survived and formed colonies. We propagated the surviving cells in drug free medium and found that these long term cultured drug survival cells (abbreviated as ltDSCs) retained a mesenchymal-like cell morphology, and expressed high levels of EMT-related molecules such as vimentin, twist and ${\beta}$-catenin. The expression of chromatin reprogramming factors, Oct4 and c-myc, were also higher in ltDSCs than parental cells. We further demonstrated that the protein level of p300 was upregulated in ltDSCs, and inhibition of p300 by siRNA suppressed the expression of vimentin. Moreover, the ltDSCs had higher colony forming ability and were more drug resistant when compared to parental cells. Our results suggested that an epigenetic mechanism is involved in the EMT of ltDSCs.

• 대한소아치과학회지
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• 제30권1호
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• pp.10-14
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• 2003

근유종은 소아에 주로 발생하는 다발성 종양으로 처음에 기술되었지만, 단독으로 발생하는 경우가 더 많고, 어느 연령층에서나 발생한다. 성인과 소아형 모두 비슷한 임상적 특성과 병리조직학적 특징을 갖는다. 이 증례는 9세 남아의 하악에 경계가 명확한 단방성의 방사선 투과성 병소로, 병리조직학적으로 방추형 세포들이 다발을 이루며 증식하며, 일부에는 혈관주피종과 유사한 부위가 관찰되었다. 면역조직화학적으로 종양세포는 vimentin과 smooth muscle actin에 양성, S-100, desmin, cytokeratin AE1/3에 음성이었다. 이 증례에서와 같이 소아에서 단방성 방사선 투과상의 고형성 종괴로 나타나는 경우 근섬유종을 감별진단에 포함시켜야 한다고 생각된다. 환자는 수술 1년이 경과한 현재까지 재발의 소견은 없지만 재발한 경우도 보고되어 있어 철저한 추적 관찰이 필요하며, 현재 #43, 44 치아의 맹출을 유도하는 중에 있다.

• Journal of Yeungnam Medical Science
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• 제16권2호
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• pp.369-375
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• 1999

1988년부터 1999년까지 영남대학교 의과대학 부속병원에서 척삭종으로 진단받은 4례를 대상으로 임상병리학적 특징을 분석하여 다음과 같은 결과를 얻었다. 환자의 연령은 57세에서 75세였고 평균연령은 63.5세였다. 4례 모두 천미골부에서 발생하였다. 종양의 평균 크기는 9.3cm 였다. 종양은 비교적 경계가 비교적 잘 지워지는 섬유성 격벽에 의해 나뉘어지는 분엽성, 점액성, 젤라틴 모습이었고, 조직학적으로 풍부한 점액성 기질과 거품모양의 공포를 갖는 담공포성 세포가 특정적으로 관찰되었다. 4례(100%)에서 종양세포들은 cytokeratin, EMA와 vimentin에 양성반응을 나타내었고, 1례(25.0%)는 S-100 protein에 양성 반응을 나타내었고, CEA에 대하여 모두 음성 반응이었다.