Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Neuronal Phenotypes and Gene Expression Profiles of the Human Adipose Tissue-Derived Stromal Cells in the Neuronal Induction

신경 분화 유도한 인체 지방조직 유래 간질세포의 신경 표현형과 유전자 발현

  • Shim, Su Kyung (Department of Plastic Surgery, College of Medicine, The Catholic University of Korea) ;
  • Oh, Deuk Young (Department of Plastic Surgery, College of Medicine, The Catholic University of Korea) ;
  • Jun, Young Joon (Department of Plastic Surgery, College of Medicine, The Catholic University of Korea) ;
  • Lee, Paik Kwon (Department of Plastic Surgery, College of Medicine, The Catholic University of Korea) ;
  • Ahn, Sang Tae (Department of Plastic Surgery, College of Medicine, The Catholic University of Korea) ;
  • Rhie, Jong Won (Department of Plastic Surgery, College of Medicine, The Catholic University of Korea)
  • 심수경 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 오득영 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 전영준 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 이백권 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 안상태 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 이종원 (가톨릭대학교 의과대학 성형외과학교실)
  • Received : 2006.11.08
  • Published : 2007.01.10
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Abstract

Purpose: Human adipose tissue-derived stromal cells(hADSCs) can be expanded in vitro and induced to differentiate into multiple mesenchymal cell types. In this study we have examined various neuronal phenotypes and gene expression profiles of the hADSCs in the neuronal induction. Methods: The hADSCs were isolated from human adipose tissue and they were characterized by the flow cytometry analysis using CD13, CD29, CD34, CD45, CD49d, CD90, CD105 and HLA-DR cell surface markers. We differentiated the hADSCs into the neuronal lineage by using chemical induction medium and observed the cells with contrast microscopy. The immunocytochemistry and western blotting were performed using the NSE, NeuN, Trk-A, Vimentin, N-CAM, S-100 and ${\beta}$-Tubulin III antibodies. Results: The hADSCs were positive for CD13($90.3{\pm}4%$), CD29($98.9{\pm}0.7%$), CD49d($13.6{\pm}6%$), CD90 ($99.4{\pm}0.1%$), CD105($96%{\pm}2.8%$) but negative for CD34, CD45 and HLA-DR. The untreated cultures of hADSCs predominately consisted of spindle shaped cells and a few large, flat cells. Three hours after the addition of induction medium, the hADSCs had changed morphology and adopted neuronal-like phenotypes. The result of immunocytochemistry and western blotting showed that NSE, NeuN, Trk-A, Vimentin, N-CAM, S-100 and ${\beta}$-Tubulin III were expressed. However, NSE, NeuN, Vimentin were weakly expressed in the control. Conclusion: Theses results indicate that hADSCs have the capabillity of differentiating into neuronal lineage in a specialized culture medium. hADSCs may be useful in the treatment of a wide variety of neurological disorders.

Keywords

Acknowledgement

Supported by : 가톨릭세포치료사업단

References

  1. Deng YB, Yuan QT, Liu XG, Liu Y, Liu ZG, Zhang C: Functional recovery after rhesus monkey spinal cord injury by transplantation of bone marrow mesenchymalstem cell-derived neurons. Clin Med J(Engl) 118: 1533, 2005
  2. Lee IW: Stem cells and neurosurgery. J Korean Neurosurg Soc 33: 1, 2003
  3. Young HE, Mancini ML, Wright RP, Smith JC, Black AC Jr, Reagan CR, Lucas PA: Mesenchymal stem cells reside within the connective tissues of many organs. Dev Dyn 202: 137, 1995
  4. Woodbury D, Schwarz EJ, Prockop DJ, Black IB: Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res 61: 364, 2000
  5. Sanchez-Ramos J, Song S, Cardozo-Pelaez F, Hazzi C, Stedeford T, Willing A, Freeman TB, Saporta S, Janssen W, Patel N, Copper DR, Sanberg PR: Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp Neurol 164: 247, 2000
  6. Filip S, Mokry J, English D, Vojacek J: Stem cell plasticity and issues of stem cell therapy. Folia Biol(praha) 51: 180, 2005
  7. Young HE, Mancini ML, Wright RP, Smith JC, Black AC Jr, Reagan CR, Lucas PA: Mesenchymal stem cells reside within the connective tissues of many organs. Dev Dyn 202: 137, 1995
  8. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrlck MH: Multilineage cells from human adipose tissue: implications for cellbased therapies. Tissue Eng 7: 211, 2001 https://doi.org/10.1089/107632701300003241
  9. Lee RH, Kim B, Choi I, Kim H, Choi HS, Suh K, Bae YC, Jung JS: Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue. Cell Physiol Biochem 14: 311, 2004 https://doi.org/10.1159/000076921
  10. Mendrick DL, Kelly DM: Temporal expression of VLA-2 and modulation of its ligand specificity by rat glomerular epithelial cells in vitro. Lab Invest 69: 690, 1993
  11. Majumdar MK, Banks V, Peluso DP, Morris EA: Isolation, characterization, and chondrogenic potential of human bone marrow-derived multipotential stromal cells. J Cell Physiol 185: 98, 2000
  12. Bartholomew A, Sturgeon C, Siatskas M, Ferrer K, McIntosh K, Patil S, Hardy W, Devine S, Ucker D, Deans R, Moseley A, Hoffman R: Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo. Exp Hematol 30: 42, 2002
  13. Tse WT, Pendleton JD, Beyer WM, Egalka MC, Guinan EC: Suppression of allogeneic T-cell proliferation by human marrow stromal cells: implications in transplantation. Transplantation 75: 389, 2003 https://doi.org/10.1097/01.TP.0000045055.63901.A9
  14. Mullen RJ, Buck CR, Smith AM: NeuN, a neuronal specific nuclear protein in vertebrates. Development 116: 201, 1992
  15. Encinas M, Iglesias M, Llecha N, Comella JK: Extracellular- regulated kinases andphosphatidylinositol 3-kinase are involved in brain-derived neurotrophic factor-mediated survival and neuritogenesis of the neuroblastoma cell line SH-SY5Y. J Neurochem 73: 1409, 1999