• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.372-375
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• 2000

Lactobacillus cristatus KLB 46 isolated from Korean woman was grown on supplemented whey medium and medium compositions were optimized for maximum viable cell count. Among the nitrogen sources tested, beef extract yielded the highest viable cell number. When corn steep liquor was applied as an additional nitrogen source, the viable cell number was highest $(3.11{\times}10^9\;CFU/ml)$ in the medium containing 50g/ l corn steep liquor and 10g/ l beef extract. The highest viable cell $count(5.00{\times}10^9\;CFU/ml)$ was obtained from the supplemented whey medium that contains beef extract(10g/ l ), corn steep liquor(50g/ l ), tween 80(0.1%, v/v) and trace amounts of sodium acetate(5g/ l ), dipotassium phosphate(2g/ l ), magnesium sulfate(0.1g/ l ), and manganese sulfate (0.05g/ l ).

• 한국축산식품학회지
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• 제21권2호
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• pp.133-141
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• 2001

Adherence of probiotic bacteria to intestinal epithelium is found to be the most principal characteristics among the various physiological functionality. This study was conducted to investigate the effect of bifidobacterial growth properties and condition on the Caco-2 cell adherence and to construct a basic data on adherence-related research. Among 20 strains of bifidobacteris tested, when measured by cell surface hydrophobicity(CSH) and cell agglutination(CA), Bifidobacterium bifidum ATCC29521, Bif. adolescentis K8, and Bif. infantis K9 were selected. Using these strains, variations of Caso-2 cell adherence depending upon experimental condition were analyzed. The results obtained are as follows : Even though Bif. bifidum ATCC29521, Bif. adolescentis K8, and Bif. infantis K9 reached more 85% cell surface hydrophobicity there was no significant difference in cell agglutination, when reached 31.54$\pm$0.54mg/ml. By direct count method for adherence, viable cell count of M3, K1, K2, K8, K9 and K10 reached more 100 counts per 100 Caco-2 cells. When Bif. bifidum ATCC29521, Bif. adolescentistis K8, and Bif. infantis K9 were used to compare the adherence depending upon viable cell counts, reaction time, and growth phase, the more viable cell count, and the more adhered cell counts, the less adherence percentage. In addition, there was no difference in adherence percentage of bifidobacteria when bifidobacteria was incubated from 1 to 8 hrs after Caco-2 cells already formed monolayer. Considering of the effect of growth phase of bifidobacteria on adherence variation, all strains showed the highest adherence during the early stage of stationary phase. In conclusion, adherence of bifidobacteria was affected by strain specificity, viable cell count, and growth activity.

• KSBB Journal
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• 제26권6호
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• pp.529-532
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• 2011

Whey based medium was optimized for the production of viable Lactobacillus crispatus KLB 46 isolated from the vagina of Korean women. Among the various nitrogen sources such as yeast extract, beef extract, and proteose peptone no. 3 supplemented to whey, beef extract showed the highest viable cell production. The addition of Tween 80 to the whey based medium increased viable cell concentration. As beef extract supplementation is not economically attractive, corn steep liquor was added as a supplementary nitrogen sources. When corn steep liquor was supplied with beef extract with the ratio 5 : 1, the viable cell count was $3.11{\times}10^9$ CFU/mL. Also, the addition of mineral salts containing sodium acetate (5 g/L), potassium phosphate dibasic (2 g/L), magnesium sulfate (0.1 g/L) and manganese sulfate (0.05 g/L) to the whey medium increased viable cell count further ($5.00{\times}10^9$ CFU/mL).

• Journal of Microbiology and Biotechnology
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• 제6권2호
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• pp.128-131
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• 1996

Lactobacillus acidophilus KFRI 233 (of human origin) exhibited a high tolerance to bile. The maximum cell yield was 6.6${\time}10^9 CFU$ per gram of whey in a 5.0% whey medium. Cell growth was improved with the addition of 0.5% thiotone and 0.25% calcium carbonate. Cell growth reached a maximum level of 5.4${\times}10^8$ CFU/ml at 20 h. Eighty-nine percent of the viable cells in the centrifuged concentrate survived freezing at $-70^{\circ}C$ and this frozen concentrate showed no reduction in the viable cell count after 30 days at $-70^{\circ}C$. Eight percent of the viable cells survived freeze-drying after the addition of 1 g/l sodium carbonate before harvesting by centrifuging and this freeze-dried concentrate showed only a slight reduction in the viable cell count after 30 days at $4^{\circ}C$.

• 미생물학회지
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• 제42권3호
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• pp.205-209
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• 2006

호소수내의 '살아있는 세균'을 측정하기 위하여 quantitative direct viable count (qDVC) 방법을 적용하였다. qDVC방법에 적용되는 최적 glycine 농도는 2%였으며, '살아있는 세균'을 계수하는데 있어서 평판계수법, CTC법 보다는 qDVC 방법이 보다 효과적이라는 것을 확인하였다. qDVC방법으로 '살아있는 세균'을 측정한 결과 다른 두 방법보다 $2.4{\sim}6.0$배 높은 값이었다. 또한 qDVC방법은 '살아있는 세균'을 죽은 세포 또는 휴면세포와 쉽게 구별할 수 있었다.

• 한국식품영양학회지
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• 제6권3호
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• pp.208-210
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• 1993

In order to know the influences of heat treatment of yoghurt on pH, $\beta$-galactosidase and viable cells, yoghurt sample was made by general method with Lactobacillus bulgaricus and Streptococcus thermophilus, and the changes in pH, $\beta$-galactosidase-activity and viable cell-count were determined during heating at 55$^{\circ}C$ and 7$0^{\circ}C$. The pH of yoghurt was not changed when the yoghurt was heated at 7$0^{\circ}C$, but at 55$^{\circ}C$ it decreased slightly. The stability of $\beta$-galactosidase was not affected markedly by heat treatment at 55$^{\circ}C$, but was rapidly inactivated at 7$0^{\circ}C$. The heat treatment of yoghurt at 55$^{\circ}C$ had the halb of viable cell in 1 hour, but the heat treatment at 7$0^{\circ}C$ had considerable effect on viable cell in 5 minutes.

• 대한치과보철학회지
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• 제40권5호
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• pp.525-535
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• 2002

The purposes of this study were to evaluate the adherence of bacteria on various denture base resin materials and effects of chitosan, added to denture base materials on bacterial adherence. PMMA denture base resin such as heat-cured Vertex-RS, self-cured Vertex-SC and 4-META denture base resin such as heat-cured Meta-Dent, self-cured Meta-Fast were used in this study Samples were divided into two groups the denture base resin with chitosan, without chitosan Streptococcus mutans and Lactobacillus casei were used in this study. The surface of samples was observed by SEM. When chitosan was added to M17 and MRS broth, viable cell count of bacteria was reduced. Viable cell count of Streptococcus mutans on the samples decreased as follows : Meta-Dent, Vertex-SC, Meta-Fast, Vertex-RS. Viable cell count of Lactobacillus casei on the samples decreased as follows: Vertex-RS, Meta-Dent, Meta-Fast, Vertex-SC. The resin with chitosan showed lower adherence of bacteria than without chitosan. The images of SEM showed that the surface of the resin with chitosan was rougher than that of without chitosan. These results showed that the denture base resin materials with chitosan have rougher surface than without chitosan, but less bacteria adhered on them.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권2호
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• pp.128-132
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• 2001

Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a string antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63h(sup)-1 and the highest viable cell count (1.9$\times$10(sup)9 CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53$\times$10(sup)9 CFU/mL) was obtained at a dilution rate of 0.32h(sup)-1, and was 7.33$\times$10(sup)11 CFU L(sup)-1 h(sup)-1, that is approximately 5 times higher than that obtained from batch culture.

• 한국식품위생안전성학회지
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• 제37권3호
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• pp.173-180
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• 2022

오징어의 품질을 평가하기 위한 여러 항목 들이 있으나 현장에서 소비자 및 유통업자들의 경우 주관적인 관능평가에 의존하고 있으며 소비자의 어류 구매의사 결정에 부패취가 직접적인 영향을 미칠 수 있다. 따라서 관능적 평가를 입증 할 수 있는 과학적인 품질인자를 제시하고자 하였으며, 온도와 저장시간에 따른 상호관계를 분석하였다. 과학적 품질인자로는 TVC, Pseudomonas spp., pH, VBN 으로 선정하였으며 세 가지 온도 조건에서 보관하였다. 이취발생검지시점은 R-index를 이용해 측정하고 kinetic 모델링을 진행하였다. 모델링 결과 활성화 에너지는 이취발생검지 시간이 51.210 kJ/mol, TVC는 42.888 kJ/mol, Pseudomonas spp.는 50.283 kJ/mol, VBN은 72.594 kJ/mol, pH는 41.990kJ/mol 으로 나타났다. Pseudomonas spp., TVC, pH, VBN 순으로 이취발생검지 시간의 활성화 에너지에 근사하였으며, 특히 Pseudomonas spp.는 이취발생검지 시간의 활성화 에너지와 가장 유사하였다. 따라서 오징어의 이취발생 indicator는 이취발생시간과 가장 유사한 양상을 나타내는 Pseudomonas spp.로 판단 할 수 있었다.

• Preventive Nutrition and Food Science
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• 제16권4호
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• pp.386-389
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• 2011

Previous studies have confirmed that fermented whey produced by Leuconostoc mesenteroides CJNU 0147 or Lactobacillus casei CJNU 0588 display bifidogenic growth stimulator (BGS) activity. The present study sought to determine if the strain itself can improve the growth of bifidobacteria in co-cultures. In reinforced clostridial medium (RCM), both strains stimulated the growth of a Bifidobacterium lactis strain during the exponential phase and also stimulated the growth during almost all growth phases in whey broth. Fermented whey containing viable Leu. mesenteroides CJNU 0147 and L. casei CJNU 0588 cells maintained viability of the B. lactis strain stored at $10^{\circ}C$ in MRS broth. Viable cell count of the B. lactis strain without the fermented whey was decreased to 5.6 log cfu/mL after 15 days, whereas that of the strain with the fermented whey was slightly increased to 7.1 log cfu/mL as compared with initial viable cell count of 6.9 log cfu/mL.