• 한국초지조사료학회지
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• 제20권4호
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• pp.275-284
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• 2000

To investigate the effect of Mycorrhiza (Glomus intradics) and Rhizobium inoculation on the N, P utilization and the growth of Alfalfa (Medicago sativa L.), four treatments (non-inoculation, control ; Mycorrhiza inoculation, M ; Rhizobiurn inoculation, R and Mycorrhiza and Rhizobium inoculation, M+R) were applied. The associated analyses were carried out on the early vegetative growth stage (DAS 56, 56 days after seeding) and on the early flowering stage (DAS 126). The decreased rate of total N and P content in soil, with advancing plant growth, was relatively higher in the mycorrhiza andor rhizobium treatments than control. The content of available phosphorus in soil at DAS 126 increased by about 40 % in M and M+R treatment compared to control (141.0 mg PzOg'kg DM), while non-significant changes was observed in R treatment. Total N uptake and P uptake in the control at DAS 126 were 33.91 mglplant and 2.42 mdplant, respectively, about 21, 50 and 51 % of increases in total N uptake and 30, 11 and 47 % of increases in total P uptake were estimated in M, R and M+R treatment. Comparing to control, dry matter yield significantly increased by 8, 27 and 28 %, and crude protein yield also by 21, 42 and 39 %, respectively, in M, R and M+R treatment. The present data indicated that mycorrhiza orland rhizobium inoculation improved N, P utilization, and consequently increased the yield of alfalfa. (Key words : Alfalfa, Mycorrhiza, Rhizobium, N and P Utilization, Growth, Yield)

• Journal of Crop Science and Biotechnology
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• 제10권4호
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• pp.281-286
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• 2007

Gibberellins are growth hormones that play a pivotal role in the growth and development of plants. Present investigations were carried to check the effect of nitrogen(N) and silicon(Si) on bioactive $GA_1$ and its immediate precursor $GA_{20}$ at different growth stages of two rice cultivars with different maturity traits. It was observed that the endogenous bioactive $GA_1$ level gradually increased during vegetative stage and anthesis stage of both Junghwabyeo(early flowering cultivar) and Daesanbyeo(late flowering cultivar). However, the $GA_1$ and $GA_{20}$ content start decreasing during the seed filling stage in both rice cultivars, which indicated a possible relationship of bioactive $GA_1$ and floral development. Our results also confirmed that early 13-hydroxylation pathway was operated at all developmental stages of rice plant. Variation in the levels of the endogenous gibberellins in rice shoots were measured by GCMS-SIM using $^2H_2$-labeled gibberellins as internal standards. Combined application of N and Si enhanced growth parameters and reduced lodging index of both rice cultivars. It was thus concluded that the level of physiologically active $GA_1$ increased during vegetative and early reproductive stage, but starts declining at seed filling stage.

• 생물환경조절학회지
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• 제31권3호
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• pp.188-193
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• 2022

배초향은 잎을 식용으로 이용하는 작물로 영양생장을 유지하여 잎의 품질과 수량을 높이는 것이 중요하다. 작물의 생장이 영양생장에서 생식생장으로 전환될 경우 잎의 발달이 저하되어 상품성이 하락할 수 있다. 따라서 본 연구는 광주기에 따른 배초향 식물의 개화 특성 및 화서 발달을 조사하여 잎 생산을 위한 적정 광주기를 선발하기 위해 수행되었다. 배초향 종자를 2021년 11월 29일에 105구 트레이에 파종하여 본엽이 4매 발생한 배초향 식물체를 2021년 12월 27일에 각각 포트에 정식하였다. 정식 후 명기/암기 시간을 10/14hrs, 12/12hrs, 14/10hrs, 16/8hrs로 조절하여 처리하였다. 정식 19일 후 광주기가 짧았던10/14hrs에서 첫 개화를 확인하였으며, 12/12hrs에서 20일, 그 외 처리에서는 21일 이후 개화하는 것을 확인하였다. 또한, 명주기가 암주기에 비해 짧을수록 화서의 발달이 증가하는 것을 통해 본 식물이 양적 단일식물의 특성을 가짐을 확인하였다. 배초향의 생육은 광주기가 길수록 엽장, 엽수, 지상부의 생체중 및 건물중, 엽면적이 증가하는 것으로 나타났다. 따라서, 명주기를 14시간 이상으로 처리하는 것이 배초향의 엽채류로서의 생산성을 높이는 데 적합할 것으로 판단된다.

• ALGAE
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• 제26권4호
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• pp.327-331
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• 2011

An analysis of the vegetative and reproductive anatomy of Sargassum lapazeanum was performed based on extensive sampling in three areas within its geographical range: a) San Juan de la Costa, b) Punta Calera, c) Punta Machos. Material was collected from low intertidal to subtidal populations and evaluated based on variations present within and between populations. Our goal was to comprehensively analyze the features of the vegetative and reproductive anatomy of this species. Our observations showed that this species has cauline, which is anatomically composed of three tissues layers. This species is dioic, with male and female conceptacles in the same receptacle but with different maturation times. The present study confirmed the presence of cauline in this species; this structure was previously described for some species in the Gulf of California, such as Sargassum horridum, and for several species in Japan. This structure could be responsible for the growth of primary branches and may also generate a new plant. This trend might explain the perennial nature of this species at the population level. Field and laboratory experiments could help to delimit the ecophysiological conditions under which this structure starts to develop.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.157-167
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• 1987

The have been many reports that phenoloxidase are correlated with development in many fungi. C. congregatus, one of nushroom-forming basidiomycetes, which requires light for its development also has phenoloxidases. In C. congragatus, there are two sets of membrane-associated phenoloxidase (PHO I and PHO II) which are differentiated by their isozyme patterns, and each enzyme set consists of two different subtrate specific enzyme protein; o-tolidine reacting enzyme, and DOPA reacting enzyme. PHO I which is localized by a protoplast-concanavalin A technique by using a new solidifying agent, Pluronic Polyol F 127, instead of agar appears in the vegetative hyphae, and PHO II appears at the early primordial stage on agar and at the sclerotial stage of liquid shake cultures. Inhibition of PHO I with the enzyme inhibitors inhibits mushroom formation as well as melanization of the vegetative hyphae at concentrations which do not inhibit the vegetative growth. PHO I deficient mutants do not form mushrooms or melanins, and the mutants show abnormal nuclear migration patterns. PHO II has roles; possibly cementing the adjacent hyphae during the actual three dimensonal structure formation, and melanizing mushrooms and sclerotia. The possible roles of PHO I in the light reception complex and in melanin formation, the function of malanin, and possible roles of postulated post translational modifying enzymes which regulate the phenoloxidases, nuclear migration pattern, and self-nonself recognition mechanism are discussed.

• ALGAE
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• 제20권2호
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• pp.141-150
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• 2005

To make a preliminary identification of the gracilarioid plant attached to cultivation ropes of Undaria pinnatifida and establish a method of cultivating this plant, the first taxonomic and cultivation studies on this species in Korea were conducted. This gracilarioid plant was identified from its morphological and anatomical features, as Gracilaria chorda. Growth tests using the 10, 20, and 30 cm cuttings of axes of G. chorda were performed twice, from May 3 to August 21, 2002 and from December 15, 2002 to April 3, 2003 in Ihoijin aquafarm, Hoijin, Jangheung, Jeollanamdo, Korea. In the first growing test, the thallus length of the 10, 20, and 30 cm cuttings increased twelve-fold, ten-fold, and seven-fold; the wet weight increased 81-fold, 60-fold, and 41-fold; the numbers of more than 10 cm-long branches increased 3.8-fold, 5.2-fold, and 6.1-fold, respectively. In the second growth test, the thallus length of the 10, 20, and 30 cm cuttings increased seven-fold, 5.5-fold, and four-fold; the wet weight increased 81-fold, 53-fold and 36-fold; the number of branches increased 3.8-fold, 7.3-fold, and 6.6-fold, respectively. The cultivation of G. chorda by vegetative regeneration using cuttings of thallus axes was successful for the first time in Korea.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.51-51
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• 2022

Water dropwort (Oenanthe stolonifera DC.) is a perennial herbaceous plant that grows wild throughout Korea. As of 2020, 24,819 tons (51.7% of open field, 48.3% of facility) are produced in 1,278 ha (59.9% of open field, 40.1% of facility) nationwide. Water dropwort, which is rich in nutrients such as vitamins and iron, is mainly cultivated by vegetative propagation method using local traditional species, however, seed propagation and breeding of cultivars are insufficient so far. Since securing, propagating and continuous characterization of various genetic resources are required to breed new cultivars, this study was conducted to compare the main characteristics of domestic genetic resources and to improve their utilization. Growth characteristics such as plant height, fresh weight, plant type, and flowering date were investigated for the 89 varieties of genetic resources owned by Jeollabuk-do Agricultural Research and Extension Services after vegetative propagation in the individual pots. Also, the morphological image information of leaves and flowers was constructed. Genetic resources were collected nationwide and originated in 30 regions including Hwaseong, Siheung and Jeju. Their plant types could be classified into straight, intermediate and creeping types according to their morphological characteristics, and at this time, the number of intermediate types accounted for the largest proportion. Flowering was carried out under high-temperature and long-day conditions in summer. According to the flowering date, they could be classified into early, middle and late flowering varieties, and at this time, the middle flowering varieties occupied the largest proportion. As a result of the investigation of vegetative growth characteristics, varieties with long plant height, heavy fresh weight and thick stem thickness were identified. Along with this result, it is thought that classification and selection of genetic resources for various purposes will be possible through additional investigations such as analysis of components and antioxidant activity. Moreover, it is judged that such results can be used as basic data for breeding new water dropwort cultivars in the future.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1993년도 추계 학술대회지
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• pp.40-41
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• 1993

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.164.2-164
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• 1998

No Abstract, See Full Text

• 한국균학회지
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• 제22권2호
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• pp.153-159
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• 1994

새로운 식용버섯 자원인 개암버섯(N. sublateritium)의 균사생장에 관한 배양적 조건을 구명하고자 시험을 수행한 결과는 다음과 같다. 1. 균사배양에 적합한 온도 및 pH는 각각 $25^{\circ}C$, 5.0-6.0의 범위였다. 2.균사배양시 양호한 탄소원으로서 단당류는 sorbitol, d-ribose 다당류는 starch, inulin, dextrin등이 있다. 3. 질소원으로 양호한 것은 무기태 질소인 질산칼륨, 암모니아태 질소인 asparagine, leucine, phenylalanine 등이었다. 4. 탄소원인 sorbitol 농도를 1-2%로 고정했을 때 적합한 C/N 비는 50이었고 질소원 농도가 높아질수록 균사생장은 부진하였다. 5. 개암버섯 균사생장에 필요한 무기염류는 비교적 다양하였고 $KH_2PO_4,\;MgSO_4{\cdot}7H_2O,\;CaCl_2{\cdot}2H_2O$의 최적농도는 각각 0.08%, 0.03%, 0.01%로 낮은 경향이었다. 6. 유기산으로 양호한 것은 fumaric, maleic, tartaric, gallic acid 등이었다.