• 한국지역사회생활과학회지
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• 제25권1호
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• pp.5-18
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• 2014

This study examines the changes in the physicochemical property and antioxidant activity of six types of vegetables (carrots, crown daisy, mugwort, cabbages, onions, and garlic) based on heat-treated cooking. According to the results, proximate contents (crude protein, fat, and ash) were lower in blanched samples than in untreated samples. Untreated cabbages showed the highest level of total dietary fiber content, which decreased by blanching and increased by high-temperature/high-pressure (HTHP) treatment. Noteworthy is that, in the case of soluble dietary fiber, blanched crown daisy and mugwort showed significantly high levels of 12.0 g and 7.3 g per 100 g(dry basis). There was no significant change in tocopherol content in heat-treated samples. The highest levels of total polyphenol and flavonoid content were 6.73 g and 5.51 g per 100 g, respectively, in the mugwort sample with HTHP treatment at $130^{\circ}C$ for 2 h. The water extract of mugwort with HTHP treatment had the strongest antioxidant effect based on three bioassays (SOD, DPPH, and ABTS). These results indicate the relative correlation between the level of physiologically active content and antioxidant activity and suggest new insights into ingredients for developing functional foods.

• 한국응용과학기술학회지
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• 제17권4호
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• pp.213-225
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• 2000

Sophorolipids were biosynthesized using a strain of yeast, Torulopsis bombicola ATCC22214. It has been reported that this yeast gives the highest yields for the production of biosurfactant sophorolipids. Hence, this yeast was used in this study. One of the objectives of this study is to increase the yield of the sophorolipid synthesis. To meet this end, basic culture medium was formulated on the basis of literature research to-date. When this medium was used, the increase in yield from 15% to 150% was observed compared to using the media in the literature. To examine how the interfacial characteristics of sophorolipids change with substrate, glucose (the first carbon source) was maintained in the media and after being cultured for three days, the second carbon sources such as alkanes, vegetable oils, alcohols or organic acids were added. The whole broth was extracted twice with ethyl acetate and the extract was analyzed by thin layer chromatograhy(TLC). After qualitative analyses by TLC, surface tensions of sophorolipids were measured by the Wilhelmy plate method and critical micelle concentration(CMC) was determined using these surface tension data. Also, interfacial tensions were measured by the spinning drop method and emulsions of the three-component water/decane/sophorolipid system were tested. Sophorolipids were effective and efficient in terms of surface tension reduction and CMC, but they were ineffective as emulsifiers because emulsions were separated within 30 minutes.

• 디자인학연구
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• 제14권2호
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• pp.245-252
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• 2001

본 연구에서는 현재 국내에서 사용되고 있는 냉장고의 레이아웃(layout)이 우리의 식생활문화와 차이가 있다는 가정 하에, 국내의 사용자들을 대상으로 비디오 에쓰노그래피(Video Ethnography : 비디오를 이용한 문화기술학, 흑은 민족지학)를 활용하여 사용자의 냉장고 사용행태를 관찰하고 분석하여 우리의 식생활문화에 적합한 새로운 냉장고 레이아웃을 제안하여 사용편의성을 개선하는 것을 중심으로 논의 하였다. 분석결과에 의하면, 현재 국내에서 사용되고 있는 대부분의 냉장고는 고내 선반의 레이아웃(layout)이 국내 사용자들의 식생활 문화에 따른 사용행태와는 맞지 않는 점을 가지고 있으며, 이를 개선하기 위한 방법으로는 현재의 냉장실 세번째 단과 제일 하단인 야채실의 위치를 맞바꾸는 방법을 제안할 수 있다. 이런 방법을 통해 냉장고 각 부위에 적합한 용도를 지정함으로써 사용자의 편의성을 향상시킬 수 있다. 냉장고와 같이 생활 문화와 밀접한 관련이 있는 제품의 내재하는 문제 요소를 추출하기 위해서는 이와 같이 그 제품과 관련된 생활 문화에 대한 이해가 우선되어야 하며, 이를 위한 방법으로는 본 연구에서 사용된 비디오 촬영를 통한 사용행태 분석 방법이, 미리 예상하지 못한 문제 요소를 발견할 수 있다 는 점에서 유효함을 알 수 있었다.

• 한국약용작물학회지
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• 제26권3호
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• pp.205-213
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• 2018

Background: Since the revised Ginseng Industrial Act was passed, ginseng sprouts have become a new medicinal vegetable for which there is high consumer demand. However, the existing amount of research and data on ginseng production has not kept pace with this changed reality. Methods and Results: In this study we analyzed the changes in the amounts of ginsenosides in different parts of growing ginseng sprouts during the period from when organic seedlings were planted in nursery soil until 8 weeks of cultivation had elapsed, which was when the leaves hardened. In the leaves, ginsenoside content increased 1.62 times with the panaxadiol (PD) system and 1.31 - 1.56 times with the panaxatriol (PT) system from 7 to 56 days after transplantation. During the same period, the total ginsenoside content of the stems decreased by 0.66 - 0.91 times, and those of the roots increased until the $21^{st}$ day, and then underwent steep declines. The effect of fermented press cake extract (FPCE) and tap water (TP) on the total amount of ginsenoside per plant were similar, and could be represented with the equations $y=1.4330+0.2262x-0.0008x^2$ and $y=0.9555+0.2997x-0.0031x^2$ in which y = ginsenoside content x = amount of and on the total amounts of FPCE or TP, respectively after 26.4 days, however, the difference between ginsenoside content with FPCE and TP widened. Conclusions: These results suggested that the amounts of ginsenosides in different parts of ginseng varied with the cultivation period and nutrient supply. These findings also provide fundamental data on the distribution of ginsenosides among plant parts for 2-year-old ginseng plants in the early-growth stage.

• KSBB Journal
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• 제17권4호
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• pp.333-338
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• 2002

생분해성 고분자 합성물질의 원료가 되는 젖산을 주로 생산하는 균을 김치로부터 분리하였다 SEM을 사용하여 관찰한 분리한 균의 형태는 연쇄간균이었고 165 rRNA sequencing 에 의해 계통발생학적 분석의 결과 Lactobacillus casei KH-1로 명명하였다. 1. casei KH-1는 g1ucose를 젖산으로 98%를 전환시키는 호모발효균이었으며, D(-)와 L(+)-이성질체를 각각 7%와 93%를 포함하는 racemic mixtrure로 젖산을 생산하였다. L. casei KH-1은 다른 lactobacillus속과는 달리 세포의 성장과 젖산의 생산에 기질로서 lactose를 이용하지 못하였지만 glucose의 이용은 이전의 연구들과 비교할 때 효과적이었다. Lactobacillus속의 배양에 사용되는 MRS배지의 질소원을 저렴한 원료 즉, YE와 CSL를 1:1로 첨가한 배지로 대신하여 최대의 젖산수율(1.09 mole .mole$^{-1}$)을 얻어 젖산발효에 효과적인 질소원의 대체가 이루어졌다

• 한국조리학회지
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• 제17권2호
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• pp.231-243
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• 2011

본 연구는 오디 생산농가 호수와 재배면적이 점차로 증가 하고 있는 추세에서 산업체종사자와 전공 관련 학생들을 중심으로 오디의 이용도를 높이기 위한 연구이다. 설문조사를 통해 과실을 이용한 유사형태의 가공제품 이용횟수를 조사한 결과 산업체 종사자는 일주일에 3-6회 4 1.7%로 사용이 많았다. 오디 이용도를 높이기 위한 가공제품개발 형태는 산업체는 시럽과 냉동 퓨레, 잼의 형태로 전공 관련 학생은 농축액, 잼, 시럽 순으로 선호하였다. 이러한 결과에 기초한 제품개발형태로는 시럽, 무설탕 농축액, 잼 순으로 제품 개발을 할 수 있으리라 사료된다. 제과품목으로는 냉장제품, 빵, 케이크류의 제품을 개발할 필요성이 제기된다. 오디가공제품을 요리품목에 활용할 경우, 두 집단 모두 채소 및 고기요리와 가장 잘 어울릴 것으로 나타났으며 이는 채소와 함께 먹을 수 있는 드레싱 및 소스를 오디와 접목하여 개발할 필요성이 있으리라 사료된다.

• 동아시아식생활학회지
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• 제24권5호
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• pp.604-613
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• 2014

This study was carried out to investigate consumption patterns of health functional foods (HFF) and analyze their related factors in male workers in order to provide basic data for appropriate understanding and optimal use of HFF. The subjects were 325 shipbuilding workers aged 20~50 in the Geoje area. About 84% of subjects reported that they were currently consuming or had experience of consuming HFF. The most commonly used type of HFF was vitamins (53.5%), followed by red ginseng (28.9%) and vegetable extract (15.4%). The major reasons for consuming and not-consuming HFF were 'to recovery from fatigue' (49.5%) and 'can not trust the effects' (39.4%), respectively. The intake period was 'less than 3 months' (35.5%), average monthly expense was 'less than 50,000 won' (49.1%), and purchase place was 'store of health functional foods' (32.2%) as the most common answers. The effects after consuming HFF were 'moderate' (51.3%) as the most common answer, and 16.1% of subjects experienced side effects, such as diarrhea, indigestion and nausea. Contributing factors for selecting and desire for future use of HFF were 'effectiveness' (52.2%) and 'when necessary' (67.8%) as the most common answers. Experience of using HFF was positively correlated with age (p<0.05), marital status (p<0.05), unbalanced diet (p<0.01), concern about health (p<0.05), and fear for disease (p<0.05). Purchase of HFF was positively correlated with fear of disease (p<0.01), whereas it was negatively correlated with frequency of exercise (p<0.05). The desire for future use of HFF was positively correlated with concern about health (p<0.05), fear of disease (p<0.01) and drinking (p<0.05). In conclusion, factors affecting consumption patterns of health functional foods (HFF) were age, marital status, unbalanced diet, concern about health, fear of disease and drinking.

• Biomolecules & Therapeutics
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• 제10권4호
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• pp.240-245
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• 2002

Cancer metastasis represents the most important cause of cancer death and antitumor agents that may inhibit this process have been extensively pursued. Invasion and metastasis of malignantly transformed cells involve degradation of the extracellular matrix (ECM) components by matrix metalloproteinases (MMP), especially MMP-2 and -9. We previously showed that H-ras-induced invasive phenotype may involve MMP-2, rather than MMP-9, in MCF10A cells. In the present study, we investigated the chemopreventive effect of Aster, a widely used culinary vegetable in Korea. We screened twelve extracts from three Aster species (Aster scaber, Aster oharai and Aster glehni) for the inhibitory effect on MMP activities of H-ras MCF10A human breast epithelial cells. All of the extracts tested in this study efficiently inhibited the gelatinolytic activities of MMP-2 and MMP-9. A more prominent inhibition was observed in MMP-2 activity compared to MMP-9. Out of twelve extracts, eight extracts showed>90％ inhibition of MMP-2 activity in H-ras MCF10A cells while only one extract showed>90％ inhibition of MMP-9 activity. We selected three extracts (AO-3, AG-3 and AS-EA) for further studies since they exerted a marked inhibition in the ratio of MMP-2 to MMP-9. Treatment with AO-3, AG-3 and AS-EA in H-ras MCF10A cells caused a significant inhibition of invasive phenotype and migration, proving a chemopreventive potential of these extracts. Taken together, our results demonstrate that extracts of Aster effectively inhibit invasion and migration of highly malignant human breast cells, possibly via downregulation of MMP-2 and MMP-9.

• Journal of Microbiology and Biotechnology
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• 제31권4호
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• pp.529-539
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• 2021

The beet armyworm, Spodoptera exigua, is a serious insect pest infesting various vegetable crops. Two infectious insect viruses, baculovirus and iflavirus, are known to induce epizootics in S. exigua populations. Indeed, some laboratory colonies have appeared to be covertly infected by these viruses. Diagnostic PCR tests detected two different viruses: Spodoptera exigua multiple nucleopolyhedrosis virus (SeMNPV) and iflaviruses (SeIfV1 and SeIfV2). Viral extract from dead larvae of S. exigua could infect Sf9 cells and produce occlusion bodies (OBs). Feeding OBs to asymptomatic larvae of S. exigua caused significant viral disease. Interestingly, both SeIfV1 and SeIfV2 increased their titers at late larval stages. Sterilization of laid eggs with 1% sodium hypochloride significantly reduced SeMNPV titers and increased larval survival rate. Double-stranded RNA (dsRNA) specific to SeIfV1 or SeIfV2 significantly reduced viral titers and increased larval survival rate. To continuously feed dsRNA, a recombinant Escherichia coli HT115 expressing SeIfV1-dsRNA was constructed with an L4440 expression vector. Adding this recombinant E. coli to the artificial diet significantly reduced the SeIfV1 titer and increased larval survival. These results indicate that laboratory colony collapse of S. exigua is induced by multiple viral infections. In addition, either suppression of SeMNPV or SeIfV infection significantly increased larval survival, suggesting a cooperative pathogenicity between baculovirus and iflavirus against S. exigua.

• 산업과 과학
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• 제2권1호
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• pp.8-14
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• 2023

본 연구에서는 흑마늘 제조 시 발생하는 부산물을 이용하여 기능성 사료 첨가제룰 개발하기 위한 사전 실험으로 수행되었다. 흑마늘박의 항산화 활성과 면역증강 활성을 측정하였으며 흑마늘박의 항산화 활성을 측정한 결과 흑마늘박이 항산화 활성이 있는 것으로 나타났다. 실험에 사용된 시료중 흑마늘박을 포함한 식물성 시료의 면역증강 활성을 실험하기 위해 Nitric oxide(NO) assay 실험을 한 결과 식물성 추출물인 흑마늘박, 섬쑥부쟁이, MIX가 최고 농도에서 LPS(100%)대비 각각 흑마늘박(69.4%), 섬쑥부쟁이(35.9%), MIX(45.3%)의 NO를 생성하였다. 따라서, 흑마늘박이 항염증 효과를 함유하고 있고 흑마늘박과 섬쑥부쟁이의 최적의 혼합비를 선정한다면 항염증 효과를 함유하고 있는 사료 첨가제로써 충분한 가치가 있을 것으로 사료된다.