• The Transactions of the Korea Information Processing Society
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• v.7 no.2
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• pp.321-327
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• 2000

Geographical Information System (GIS) is composed of hardware, software, data, and methods. Among the components, data is considered to be the most important factor in GIS. In this paper, we describes HVF, the most recent technology pioneered by GIS researchers. HVF is a format for efficient usage of spatial object in order to express, save and transform in Internet GIS. Fist, we describe the definition of HVF syntax, then the implementation of Header Section Generation Module and Main Section Generation Module through the API. Second, we develop a spatial object converter for HVF to transform figures into data structures, which are compatible among different GIS applications. For this purpose, we design and implement three units of modules, which are File Manipulation, HVF Import and HVF Export Module. Since the issues of network traffic and Hangul processing in Internet GIS are important, we expecially consider development of HVF in terms of spatial data size.

• Korean Journal of Environmental Agriculture
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• v.40 no.3
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• pp.179-185
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• 2021

BACKGROUND: Tomato genome editing using CRISPR-Cas9 is being actively conducted in recent days, and lots of plant researches have been aiming to develop high valued crops by editing target genes without inserting foreign genes. Many researchers have been involved in the manipulation of the crop ripening process because fruit ripening is an important fruit phenotype for increasing fruit shelf life, taste, and texture of crops. This paper intends to evaluate target sgRNA to edit the two ripening-related genes encoding pectate lyase (PL) and phytoene synthase (Psy) with the CRISPR-Cas9 system. METHODS AND RESULTS: The CRISPR-Cas9 expression vector was cloned to target the PL (Solyc03g111690), Psy1 (Solyc03g031860), and Psy2 (Solyc02g081330) genes, which are the ripening genes of tomatoes. Tomatoes injected with Agrobacterium containing the CRISPR-Cas9 expression vector were further cultured for 5 days and used to check gene editing efficiency. As a result of the target gene sequence analysis by the next generation sequencing method, gene editing efficiency was calculated, and the efficient target location was selected for the PL and Psy genes. CONCLUSION: Therefore, this study was aimed to establish target sgRNA data that could have higher efficiency of the CRISPR-Cas9 system to obtain the delayed ripening phenotype of tomato. The developed method and sgRNA information is expected to be utilized in the development of various crops to manage its ripening processes.

• Journal of Digital Contents Society
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• v.13 no.1
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• pp.31-36
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• 2012

Since the national standard topographic map is made by DXF format which is the results of the National Geographic Information System project and lots of CAD data and scanners can be used to acquire the data easily, the imports of existing DXF file will decrease the costs and time for data acquisition process. In addition, the spatial data such as topographic map should be able to convert to the middle file format such as PostScript in order to be exported into other systems. For this purpose, I design and implement three units of modules, which are File Manipulation Module, MVF Import Module, and MVF Export Module. Finally, I verify the actual transformation of topographic map that are DXF file format by conducting the series of tests.

• Journal of the Korean Society for Precision Engineering
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• v.16 no.12
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• pp.87-98
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• 1999

This study is concentrated on the development of real-time estimation model and vision control method as well as the experimental test. The proposed method permits a kind of adaptability not otherwise available in that the relationship between the camera-space location of manipulable visual cues and the vector of manipulator joint coordinates is estimate in real time. This is done based on a estimation model ta\hat generalizes known manipulator kinematics to accommodate unknown relative camera position and orientation as well as uncertainty of manipulator. This vision control method is roboust and reliable, which overcomes the difficulties of the conventional research such as precise calibration of the vision sensor, exact kinematic modeling of the manipulator, and correct knowledge of position and orientation of CCD camera with respect to the manipulator base. Finally, evidence of the ability of real-time vision control method for manipulator's position control is provided by performing the thin-rod placement in space with 2 cues test model which is completed without a prior knowledge of camera or manipulator positions. This feature opens the door to a range of applications of manipulation, including a mobile manipulator with stationary cameras tracking and providing information for control of the manipulator event.

• Microbiology and Biotechnology Letters
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• v.23 no.2
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• pp.164-169
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• 1995

An endogenous cryptic plasmid, pBL1, which has been used to construct plasmid vectors for coryneform bacteria producing amino acids, was eliminated from Brevibacterium lactofermentum. The pBL1 was partially digested with Sau3AI and the resulting DNA fragments were subcloned into a suicide vector pEM1 which contains a kanamycin-resistant (km$^{r}$) gene. KM$^{r}$ B. lactofermentum transconjugants were obtained by conjugal transfer of the pEM1 derivatives containing pBL1 DNA fragments from Escherichia coli into B. lactofermentum. A km$^{r}$ transconjugant was analyzed to contain a plasmid pEB14, which occurred in vivo by homologous recombination between pBL1 and the conjugal-transferred plasmid. The pEB14 including the pEM1-derived km$^{r}$ gene was found to be lost concomitantly with km$^{r}$ phenotype, resulting in the construction of a pBL1-free strain of B lactofermentum. Based on transformation efficiencies and plasmid stability, the resultant pBL1- free strain is more useful than wild strain as a host cell for genetic manipulation. It could be concluded that foreign plasmid DNAs are efficiently isolated and analyzed from the pBL1-free strain because of the absence of endogenous pBL1 plasmid.

• Journal of Korean Society of Transportation
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• v.20 no.4
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• pp.109-122
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• 2002

본 연구에서는 교차로의 비용 및 특성을 고려한 도로선형최적화 모형을 유전자 알고리즘(Genetic Algorithms)을 이용하여 개발하였다. 기존의 도로선형최적화 모형은 교차로 특성을 고려하지 못해서 실제 적용에 심대한 문제점을 내재하고 있다. 본 논문에서는 특정 도로선형에 교차로 건설의 필요가 있을 경우, 민감(Sensitive)하고 지배적인(Dominating) 교차로 비용 항목들 즉, 토공비용, 보상비, 포장비, 사고비용, 지체 및 연료소모비용 등의 산정이 시도되었다. 또한 비교적 우수한 도로선형 대안을 유전자 알고리즘을 이용한 탐색과정 중에서 비효율적으로 강제 퇴화시키는 단점 보완을 위한 교차로 국소 최적화 방법(Local Optimization of Intersections)이 개발되어 기존 모형을 보완하였다. 공간상의 도로선형은 매개변수적 묘사(Parametric Representation)를 통하여 구현하였으며 벡터운영(Vector Manipulation)을 통해 교차로비용 산정의 근간인 교차점과 다른 중요점들의 좌표를 찾을 수 있었다. 개발된 교차로 비용산정 모형이 보다 정밀하게 교차로 비용을 산정함이 증명되었으며 궁극적으로는 기존의 최적화 모형의 단점을 보완할 수 있음이 제시되었다. 또한, 새로이 제시된 교차로 국소 최적화 방법이 최적대안 탐색과정의 유연성을 증대하였으며, 결과적으로 효율적인 교차로의 유지에 기여함을 알 수 있었다. 제시된 교차로 국소 최적화 방법은 추후 단일노선이 아닌 도로망 최적화시의 기초를 제시함은 주목할 만 하다. 두개의 예제에서 도출된 최적노선 및 교차로 비용 등의 검토 결과, 도로상의 교차로 건설비용은 도로선형 최적화에 큰 영향을 미치는 실질적이며 민감한 비용 항목임이 검증되었으며 이는 도로선형최적화 모형이 교차로 비용을 반드시 검토 및 평가할 수 있어야 함을 반증한다.

• 전기의세계
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• v.29 no.4
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• pp.247-255
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• 1980

In this study, an application of Finite Element Method which, in principle, based on variational calculus has been presented for the two-dimensional analysis of magnetic flux distribution in the shell type core of single phase transformer. The necessary stationarity condition of energy functional and boundary conditions were determined under the assumptions that the electromagnetic field considered is stationary and that the effect of eddy current is negligible. In the process of application the domain of magnetic field was divided into triangle subsectional elements and then the matrix equations were constructed for the respective triangular element and for those of all after the manipulation of minimization process to the vector potential of magnetic field at the each vertex of the element. Furthermore the numerical computation for the equations was guided by the Gaussian Elimination Methods. As the results obtained, it is found that the aspect of magnetic flux distribution inside the core as well as the leakage flux profile at the vicinity of the inner leg of the core is not much different from the well-known distribution profile of magnetic flux, however, the procedure shows to possess the merit of the uniquely deterministic nature for the flux distribution at the desired points.

• Proceedings of the Microbiological Society of Korea Conference
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• 2000.05a
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• pp.58-65
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• 2000

Diarrheal diseases are a major cause of both illness and death in developing countries and are caused by rotavirus, Shigella spp., Salmonella spp., enterotoxigenic Escherichia coli (ETEC), and Vibrio spp. In this study, for the development of vaccine against diarrheal diseases caused by Shigella sonei, Salmonella typhimurium, E. coli O157, and Vibrio cholerae, cloning and nucleotide sequence analysis of genes and characteristics of their gene products in E. coli were performed. For construction of attenuated strain of S. sonnei KNIH104 and Salmonella typhimurium KNIH100, the aroA genes were cloned, respectively. The recombinant plasmid $_pJP{\Delta}A45$ containing aroA deleted region and suicide vector $(_pJP5603)$ was constructed. The aroA gene deleted mutants were constructed using this recombinant plasmid. For cloning gene encoding antigenic region of E. coli O157 KNIH317, the O-antigen synthesis gene cluster and sit gene was cloned. The E. coli XL1-Blue cells harboring this recombinant plasmid showed cytotoxicity in Vero cells. The ctx gene was cloned for tile purpose of antigenic region against V. cholerae KNIH002. Sequence analysis confirmed that the virulence gene cassette was consisted of ace, zot, ctxA and ctxB genes.

• Structural Engineering and Mechanics
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• v.40 no.2
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• pp.257-277
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• 2011

In this paper the geometrically nonlinear continuum plate finite element model, hitherto not reported in the literature, is developed using the total Lagrange formulation. With the layerwise displacement field of Reddy, nonlinear Green-Lagrange small strain large displacements relations (in the von Karman sense) and linear elastic orthotropic material properties for each lamina, the 3D elasticity equations are reduced to 2D problem and the nonlinear equilibrium integral form is obtained. By performing the linearization on nonlinear integral form and then the discretization on linearized integral form, tangent stiffness matrix is obtained with less manipulation and in more consistent form, compared to the one obtained using laminated element approach. Symmetric tangent stiffness matrixes, together with internal force vector are then utilized in Newton Raphson's method for the numerical solution of nonlinear incremental finite element equilibrium equations. Despite of its complex layer dependent numerical nature, the present model has no shear locking problems, compared to ESL (Equivalent Single Layer) models, or aspect ratio problems, as the 3D finite element may have when analyzing thin plate behavior. The originally coded MATLAB computer program for the finite element solution is used to verify the accuracy of the numerical model, by calculating nonlinear response of plates with different mechanical properties, which are isotropic, orthotropic and anisotropic (cross ply and angle ply), different plate thickness, different boundary conditions and different load direction (unloading/loading). The obtained results are compared with available results from the literature and the linear solutions from the author's previous papers.

• Proceedings of the KSRS Conference
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• 1999.11a
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• pp.75-79
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• 1999

Geo-features play a key role in object-oriented or feature-based geo-processing system. So the strategy for how-to-model and how-to-manage the geo-features builds the main architecture of the entire system and also supports the efficiency and functionality of the system. Unlike the conventional 2D geo-processing system, geo-features in 3B GIS have lots to be considered to model regarding the efficient manipulation and analysis and visualization. When the system is running on the Web, it should also be considered that how to leverage the level of detail and the level of automation of modeling in addition to the support for client side data interoperability. We built a set of 3D geo-features, and each geo-feature contains a set of aspatial data and 3D geo-primitives. The 3D geo-primitives contain the fundamental modeling data such as the height of building and the burial depth of gas pipeline. We separated the additional modeling data on the geometry and appearance of the model from the fundamental modeling data to make the table in database more concise and to allow the users more freedom to represent the geo-object. To get the users to build and exchange their own data, we devised a file format called VGFF 2.0 which stands for Virtual GIS File Format. It is to describe the three dimensional geo-information in XML(eXtensible Markup Language). The DTD(Document Type Definition) of VGFF 2.0 is parsed using the DOM(Document Object Model). We also developed the authoring tools for. users can make their own 3D geo-features and model and save the data to VGFF 2.0 format. We are now expecting the VGFF 2.0 evolve to the 3D version of SVG(Scalable Vector Graphics) especially for 3D GIS on the Web.