• Journal of Ginseng Research
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• 제41권4호
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• pp.477-486
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• 2017

Background: Our previous studies have demonstrated that ginsenoside-Rg1 can promote angiogenesis in vitro and in vivo through activation of the glucocorticoid receptor (GR). Furthermore, microRNA (miRNA) expression profiling has shown that Rg1 can modulate the expression of a subset of miRNAs to induce angiogenesis. Moreover, Rb1 was shown to be antiangiogenic through activation of a different pathway. These studies highlight the important functions of miRNAs on ginseng-regulated physiological processes. The aim of this study was to determine the angiogenic properties of Korean Red Ginseng extract (KGE). Methods and Results: Combining in vitro and in vivo data, KGE at $500{\mu}g/mL$ was found to induce angiogenesis. According to the miRNA sequencing, 484 differentially expressed miRNAs were found to be affected by KGE. Among them, angiogenic-related miRNAs; miR-15b, -23a, -214, and -377 were suppressed by KGE. Meanwhile, their corresponding angiogenic proteins were stimulated, including vascular endothelial growth factor, vascular endothelial growth factor receptor-2, endothelial nitric oxide synthase, and MET transmembrane tyrosine kinase. The miRNAs-regulated signaling pathways of KGE were then found by Cignal 45-Pathway Reporter Array, proving that KGE could activate GR. Conclusion: KGE was found capable of inducing angiogenesis both in vivo and in vitro models through activating GR. This study provides a valuable insight into the angiogenic mechanisms depicted by KGE in relation to specific miRNAs.

• 한국작물학회지
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• 제47권2호
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• pp.85-94
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• 2002

Antibodies raised against the purified p-subunit of $\beta$-conglycinin were used in immunohistochemical studies to monitor the pattern of $\beta$-conglycinin mobilization in the cotyledons during soybean [Glycine max (L.) Merr.] seed germination. Western blot analysis revealed that the break down of the $\beta$-subunit of $\beta$-conglycinin commenced as early as 2 days after seed imbibition (DAI). Concurrent with the degradation of the $\beta$-subunit of $\beta$-conglycinin, accumulation of 48, 28, and 26 kD proteolytic intermediates was observed from 2 to 6 DAI. Western blot analysis also revealed that the acidic subunit of glycinin was mobilized earlier than the basic subunit. The basic glycinin subunit was subjected to proteolysis within 2 DAI resulting in the appearance of an intermediate product approximately 2 kD smaller than the native basic glycinin subunit. In contrast to the major seed storage proteins, lipoxygenase was subjected to limited proteolysis and was detected even after 8 DAI. The first sign of $\beta$-conglycinin breakdown was observed near the vascular strands and proceeded from the vascular strands towards the epidermis. Protein A-gold localization studies using thin sections of soybean cotyledons and antibodies raised against the $\beta$-subunit of $\beta$-conglycinin revealed intense labeling over protein bodies. A pronounced decrease in the protein A-gold labeling intensity over protein bodies was observed at later stages of seed germination. The protein bodies, which were converted into a large central vacuole by 8 DAI, contained very little 7S protein as evidenced by sparse protein A-gold labeling in the vacuoles.

• Journal of Magnetics
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• 제20권1호
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• pp.40-46
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• 2015

This study investigated the accuracy of magnetic resonance angiography (MRA) and computed tomography angiography (CTA) in terms of reflecting the actual vascular length. Three-dimensional time of flight (3D TOF) MRA, 3D contrast-enhanced (CE) MRA, volume-rendering after CTA and maximum intensity projection were investigated using a flow model phantom with a diameter of 2.11 mm and area of $0.26cm^2$. 1.5 and 3.0 Tesla devices were used for 3D TOF MRA and 3D CE MRA. CTA was investigated using 16 and 64 channel CT scanners, and the images were transmitted and reconstructed by volume-rendering and maximum intensity projection, followed by conduit length measurement as described above. The smallest 3D TOF MRA measure was $2.51{\pm}0.12mm$ with a flow velocity of 40 cm/s using the 3.0 Tesla apparatus, and $2.57{\pm}0.07mm$ with a velocity of 71.5 cm/s using the 1.5 Tesla apparatus; both images were magnified from the actual measurement of 2.11 mm. The measurement with the 16 channel CT scanner was smaller ($3.83{\pm}0.37mm$) than the reconstructed image on maximum intensity projection. The images from CTA from examination apparatus and reconstruction technique were all larger than the actual measurement.

• Journal of Digestive Cancer Research
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• 제1권1호
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• pp.24-28
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• 2013

위암은 최근 50년간 5년 생존율에 있어서 미약하게만 상승되었다. 이에 따라 위암에 대한 분자생물학적 치료에 대한 여러 연구가 진행되고 있다. 이에 진행성, 전이성 위암에서의 분자생물학적 치료에 대해 살펴보고자 한다. 위암의 분자생물학적 치료의 타켓으로는 EGFR (Epidermal growth factor receptor), HER(Human epidermal growth factor receptor), VEGF (Vascular endothelial growth factor receptor)를 들 수 있다. 대표적으로 분자생물학적 치료 중 trastuzumab은 HER2과발현 환자에게 유용하게 사용될 수 있어, 기존 항암화학요법에 복합투여해 볼 수 있다. Trastuzumab와 그 밖의 여러 분자생물학적 제재에 대해 본 연구에서 살펴보고자 한다.

• Journal of Ecology and Environment
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• 제48권1호
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• pp.1-7
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• 2024

Background: Available publications (e.g., theses, scientific reports, books and papers) about the elements of the Egyptian biodiversity during 2000-2018 were collected in a progress scientific report. The publications reported in this bibliography were collected from various sources including: site of the Egyptian Universities Libraries Consortium Portal, accounts of the biodiversity specialists on Research gate, direct contact with the national experts of the Egyptian biodiversity, libraries of some universities and research centers and others. The elements of the Egyptian biodiversity are classified into different categories. Results: Up till now, a total of 20,521 species were recorded in Egypt, of which insects have the highest contribution (48.7%), followed by fungi (12.1%) and vascular plants (11.5%). In a descending order, each of amphibians, viruses, reptiles, mammals, cyanobacteria, bryophytes, and bacteria have a minor contribution (< 1%). Based on the available data, Egyptian biodiversity contributes 1.3% of the world biota, although its area contributes only 0.7% of the world area. At a global scale, the most represented groups are algae (12.22% of the world figure), followed by cyanobacteria (6.08%) and birds (4.70%). On the other hand, the less represented are amphibians (0.14% of the world figure), flora (0.84%) and insects (1.00%). Conclusions: Some suggestions are recommended for preparing a phytoplankton checklist based on the rich available publications; further studies should be carried out on the lichen biodiversity in order to prepare acceptable verified checklist for this important group. In addition, paleo-biologists should work together to publish a book dealing with the Egyptian paleo-biology, such studies will lead to high ranking the Egyptian biodiversity.

• 대한한방부인과학회지
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• 제33권3호
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• pp.60-79
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• 2020

Objectives: This review plans to assess the efficacy and effectiveness of oriental medicine for the treatment of Ovarian Hyperstimulation Syndrome (OHSS) through literature research and overview. Methods: Database searching was conducted to identify relevant randomized controlled trials (RCTs) on oriental medicine for the treatment of Ovarian Hyperstimulation Syndrome. Studies were searched from Journal of Korean Obstetrics and Gynecology, Korean Medical Database, Korean studies Information Service System, China National Knowledge Infrastructure, Cochrane library, PubMed and EmBase up to 21^st May, 2020. Results: Seventeen studies were finally selected. Fifteen studies intervened with oral Chinese herb medicine, two studies intervened with acupuncture and moxibustion. Nine studies concluded that intervention with oriental medicine significantly relieved OHSS symptoms. Three studies reporting ovary diameter, four studies reporting abdominal circumference and other four studies reporting pelvic effusion showed significant reduction compared to control groups. Six studies showed significantly shorter duration for hospitalization in intervention groups. Only one study showed significantly higher pregnancy rate. Factors related with vascular permeability and blood cell coagulation were significantly lowered in intervention groups in general. Conclusions: From seventeen studies, oriental medicine relieved OHSS symptoms and showed treatment effectiveness. Further strictly designed studies and long-term observed studies are needed to establish evidences.

• 대한한방부인과학회지
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• 제33권1호
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• pp.1-18
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• 2020

Objectives: This review is aimed at assessing the efficacy and effectiveness of oriental medicine for the prevention of Ovarian Hyperstimulation Syndrome (OHSS) through literature research and overview. Methods: Database searching was conducted to identify relevant randomized controlled trials (RCTs) on oriental medicine for the prevention of Ovarian Hyperstimulation Syndrome. Studies were searched from Journal of Korean Obstetrics and Gynecology, Korean studies Information Service System, Korean Medical Database, China National Knowledge Infrastructure, Cochrane library, PubMed and EmBase up to 7^th November, 2019. Results: Ten RCTs were finally selected. Eight studies intervened with oral Chinese herb medicine, one is intervened with Chinese medicine enema and the other with acupuncture. Eight studies concluded that intervention with oriental medicine significantly decreased OHSS incidence. Five studies showed significantly higher pregnancy rate in the intervention groups. Two studies reported higher ovulation rate and other two studies showed more maturated eggs than the control groups. Four studies showed opposite results in serum Estradiol level. Vascular Endothelial Growth Factor level was significantly lower in the intervention groups in two studies. Conclusions: From ten studies, oriental medicine reduced OHSS incidence rate and showed preventable effectiveness. Further strictly designed studies and acupuncture intervened studies are needed to establish evidences.

• 생약학회지
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• 제14권2호
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• pp.51-59
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• 1983

Experimental studies were made with Melilotus officinalis extract which was extracted from flowers and leaves of Melilotus officinalis Dsr. (Leguminosae). In this paper, acute toxicity, analgesic action, prolongation of hypnosis time by induced pentobarbital-Nain mice, antiinflammatory effect in rats and effects on isolated intestines of mice and rats were studied, The result was as follows; 1. Very low toxicity in mice. 2. Analgesic action was recognized markedly in mice. 3. Prolongation of hypnosis time induced by pentobarbital-Na in mice was shown. 4. Relaxing action was shown on the isolated ileum in mice and antagonistic action was seen on $BaCl_2-induced$ contraction of the ileum that the relaxing effect of the intestinal smooth muscle was recognized. 5.Antiinflammatory effect was shown markedly in mice. 6.Hypotensive and vaso-dilating actions due to the vascular smooth muscle relaxation were noted in rabbits.

• The Korean Journal of Ecology
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• 제24권6호
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• pp.381-384
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• 2001

A preliminary comparative histodimensional studies, such as wood to bark ratio, total area of parenchyma cells and bast fibers, height and diameter of vascular rays, pith diameter and phenological parameters, height of the plant, perimeter of the stem, dry weight of the stem and leaves have been investigated for the low and high rubber bearing Guayule plants growing in same and different climatic regions of India. A plausible correlation is found between the percentage of rubber content and anatomical characters in the investigated varieties.

• Annals of Clinical Neurophysiology
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• 제1권1호
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• pp.70-75
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• 1999

Trancranial Doppler(TCD) monitoring is a new application of ultrasonography which allows the nonivasive detection of blood flow velocity in the horizontal (M1) segment of the middle cerebral artery (MCA) and detects microembolic phenomena in the cerebral circulation. Recent studies emphasized the potential of using this technique in vascular surgery (carotid endarterectomy, cardiopulmonary bypass), interventional and intensive care setting. Although the disparity between CBF and blood flow velocity and number of microemboli could be used to prevent cerebral ischemic and embolism based on clinical studies. A reduction of more than 60% of MCA can reflex hemodynamic ischemic state and acoustic feedback of high intensity transient signals(HITS) from the TCD monitoring unit has a direct influence on surgical technique. TCD monitoring can immediately provide information about thromboembolism and hemodynamic changes, which may be a useful tool in the study and prevention of stroke.