• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.112-112
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• 2017

Rice blast caused by Magnaporthe oryzae is one of the most serious diseases that affect the quantity and quality of rice production. The use of resistant rice varieties would be the most effective way to control the rice blast. However R gene incorporation into the rice variety takes time and pathogen could overcome the R gene effects after for a while. For monitoring the rice blast resistance gene distribution in Korean varieties, the four major blast resistance genes against M. oryzae were screened in a number of Korean rice varieties using molecular markers. Of the 120 rice varieties tested, 40 were found to contain the Pi-5 gene, 25 for the Pi-9 gene, 79 for Pi-b and 40 for the Pi-ta gene. None of these rice varieties includes tested 4 R genes. 3 R genes combination, Pi-5/Pi-9/Pi-b, Pi-5, Pi-9.Pi-ta, or Pi-9/Pi-b/Pi-ta were found in 12 varieties, the rice blast disease severity were showed as resistant in the rice verities containing Pi-9/Pi-b/Pi-ta R genes combination, respectively. Also pathogenic diversity of M. oryzae isolates collected in the rice field from 2004 to 2015 in rice field in Korea were analyzed using rice blast monogenic lines, each harboring a single blast resistance gene. Compatibility of blast isolates against rice blast monogenic lines carrying the resistance genes Pi5, Pi9, Pib, and Piz showed dynamic changes by year. It indicates that pathogen has high evolutionary potential adapted host resistances to increase fitness and would lead to rice blast resistance bred into the cultivar becoming ineffective eventually.

• 대한수의학회지
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• 제48권2호
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• pp.131-137
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• 2008

Pesticides are extensively used for the control of crop pests in agriculture and forestry. Organophosphate (OP) and carbamate pesticides are especially effective for the control of a variety of harmful insects. However, these cholinesterase inhibitors are also dangerous to non-target organisms (wildlife and other animals) because of their high acute toxicity. Most poisonings by pesticides occur as a result of misuse or accidental exposure, but intentional killing of unwanted animals also occurs. At the request of a local autonomous entity, we investigated wild bird poisonings by pesticides from 2003 to 2007. The 207 suspicious samples of pesticide poisoning based on the necropsy were analyzed by GC/NPD, GC/FPD, or GC/MSD. We looked for trends in the identification of pesticides in wild birds thought to have died from poisoning. Pesticides were determined in 59% of the total samples analyzed. Phosphamidon and monochrotophos were the most common pesticides identified, which amounted to 77% of the subtotal. Other OP and carbamate pesticides were also found in various concentrations from dead wild birds. The determined rates of pesticides were as high as 86% and 76% in 2003 and 2006, respectively, during an outbreak of avian influenza in Korea.

• 한국인터넷방송통신학회논문지
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• 제17권5호
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• pp.125-130
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• 2017

PLC 등의 현장제어기기는 주요 이벤트 정보를 로깅하는 기능이 없기 때문에 사고분석이 힘들다. 따라서, PLC, IED와 같은 현장제어기기의 주요 이벤트 정보를 로깅하여, 사이버 사고 발생 시 분석이 가능한 정보 확보가 필요하다. 이벤트 로깅을 위한 현장제어기기(임베디드기기) 통신 프로토콜을 분석하기 위해서는 프로토콜 애널라이저(분석기)가 필요하다. 그러나 Wireshark와 같은 기존의 분석기는 페이로드 데이터 기반의 다양한 프로토콜 분석 및 분류가 어렵고 이벤트 로깅을 위한 대용량의 데이터 식별 및 추출을 처리하기에는 어려움이 있다. 따라서, 본 논문에서는 대용량의 이벤트 로깅을 위한 빅데이터 기반 현장제어기기 통신프로토콜 페이로드 데이터 추출을 위한 시스템을 연구개발하였다.

• 한국환경보건학회지
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• 제37권4호
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• pp.289-297
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• 2011

Objectives: To estimate the microbial contaminant load discharged from livestock farms, we randomly selected livestock farmers of cattle, swine, and fowl and collected bacterial strains from domestic animals' feces and compost samples. Recently, as multi-antibiotic-resistant bacteria and super bacteria showing resistance to a variety of antibiotics have been reported one after another, the ecological and health hazard of antibiotic-resistant bacteria is emerging as an important issue. Methods: Monitored indicator microorganism constituents were totak coliform (TC), fecal coliform (FC), and aerobic bacteria. The multi-antibiotic-resistant bacteria were identified from investigated indicator microorganisms by 16S rRNA sequencing. Results: By microbiological analysis, the largest population of aerobic bacteria ($1.5{\times}10^5$ CFU/g) was found in cattle fecal compost, and total coliforms ($1.1{\times}10^7$ CFU/g) and fecal coliforms ($1.0{\times}10^5$ CFU/g) were found primarily in swine fecal compost, while the lowest population was found in fowl fecal compost. Among the 67 strains separated from aerobic bacteria, five strains expressing high antibiotic resistance were selected in each sample. We found the multi-antibiotic resistant strains to be Shigella boydii, Staphylococcus lentus, Acinetobacter sp. and Brevibacterium luteolum. Conclusions: These results suggest that increasing numbers of multi-antibiotic-resistant bacteria in the environment have a close relation to the reckless use of antibiotics with livestock.

• BMB Reports
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• 제37권5호
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• pp.522-526
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• 2004

Prader-Willi (PWS) and Angelman (AS) are syndromes of developmental impairment that result from the loss of expression of imprinted genes in the paternal (PWS) or maternal (AS) 15q11-q13 chromosome. Diagnosis on a clinical basis is difficult in newborns and young infants; thus, a suitable molecular test capable of revealing chromosomal abnormalities is required. We used a variety of cytogenetic and molecular approaches, such as, chromosome G banding, fluorescent in situ hybridization, a DNA methylation test, and a set of chromosome 15 DNA polymorphisms to characterize a cohort of 27 PWS patients and 24 suspected AS patients. Molecular analysis enabled the reliable diagnosis of 14 PWS and 7 AS patients, and their classification into four groups: (A) 6 of these 14 PWS subjects (44%) had deletions of paternal 15q11-q13; (B) 4 of the 7 AS patients had deletions of maternal 15q11-q13; (C) one PWS patient (8%) had a maternal uniparental disomy (UPD) of chromosome 15; (D) the remaining reliably diagnoses of 7 PWS and 3 AS cases showed abnormal methylation patterns of 15q11-q13 chromosome, but none of the alterations shown by the above groups, although they may have harbored deletions undetected by the markers used. This study highlights the importance of using a combination of cytogenetic and molecular tests for a reliable diagnosis of PWS or AS, and for the identification of genetic alterations.

• Journal of Yeungnam Medical Science
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• 제21권1호
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• pp.101-107
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• 2004

Diffuse alveolar hemorrhage is a rare but serious and frequently life-threatening complication of a variety of conditions. The first goal in the management of patients with diffuse alveolar hemorrhage is to achieve or preserve stability of the respiratory status. Subsequently, the differential diagnosis is aimed at the identification of a remediable cause of the alveolar hemorrhage. The most common causes of diffuse alveolar hemorrhage with glomerulonephritis are microscopic polyangiitis and Wegener's granulomatosis, followed by Goodpasture syndrome and systemic lupus erythematosus. Microscopic polyangiitis (MPA) is a distinct systemic small vessle vasculitis affecting small sized vessels with few or no immune deposits and with no granulomatosus inflammation. The disease may involve multiple organs such as kidney, lung, skin, joint, muscle, gastrointestinal tract, eye, and nervous system. MPA is strongly associated with antineutrophil cytoplasmic autoantibody (ANCA) that is a useful serological diagnostic marker for the most common form of necrotizing vasculitis. Our report concerns a case of microscopic polyangiitis with diffuse alveolar hemorrhage in a 54-year-old man. He was admitted to our hospital due to dyspnea upon exertion and recurrent hemoptysis. Laboratory findings showed hematuria, proteinuria and deterioration of renal function. In the chest CT scan, diffuse ground glass appearance was seen in both lower lungs. A lung biopsy revealed small vessel vasculitis with intraalveolar hemorrhage and showed a positive reaction to against perinuclear ANCA. The patient was treated with prednisolone and cyclophosphamide. Chest infiltration decreased and hemoptysis and hypoxia improved. He is still being followed up in our hospital with a low dose of prednisolone.

• Asian-Australasian Journal of Animal Sciences
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• 제27권12호
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• pp.1684-1690
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• 2014

Estrogen and its receptors are essential hormones for normal reproductive function in males and females during developmental stage. To better understand the effect of estrogen receptor (ER) gene in yak (Bos grunniens), reverse transcription-polymerase chain reaction (PCR) was carried out to clone $ER{\alpha}$ and $ER{\beta}$ genes. Bioinformatics methods were used to analyze the evolutionary relationship between yaks and other species, and real-time PCR was performed to identify the mRNA expression of $ER{\alpha}$ and $ER{\beta}$. Sequence analysis showed that the ER open reading frames (ORFs) encoded 596 and 527 amino acid proteins. The yak $ER{\alpha}$ and $ER{\beta}$ shared 45.3% to 99.5% and 53.9% to 99.1% protein sequence identities with other species homologs, respectively. Real-time PCR analysis revealed that $ER{\alpha}$ and $ER{\beta}$ were expressed in a variety of tissues, but the expression level of $ER{\alpha}$ was higher than that of $ER{\beta}$ in all tissues, except testis. The mRNA expression of $ER{\alpha}$ was highest in the mammary gland, followed by uterus, oviduct, and ovary, and lowest in the liver, kidney, lung, testis, spleen, and heart. The $ER{\beta}$ mRNA level was highest in the ovary; intermediary in the uterus and oviduct; and lowest in the heart, liver, spleen, lung, kidney, mammary gland, and testis. The identification and tissue distribution of ER genes in yaks provides a foundation for the further study on their biological functions.

• 대한간호학회지
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• 제41권1호
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• pp.101-109
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• 2011

Purpose: This study was done to comparatively analyze the general education curriculum of 4-yr and 3-yr nursing schools in Korea. Methods: Ten university 4-yr nursing schools were selected based on universities in Korean Accreditation Board of Nursing 2010 or "2009 Korea's Best Universities-Top 10" published by Joong-Ang Daily. Ten college 3-yr nursing schools were selected based on colleges in Korean Accreditation Board of Nursing 2010. Results: 1) Generally 4-yr nursing schools maintained the relationships between organizational philosophy/purposes and subjects in the general education curriculum. But 3-yr nursing schools did not. 2) In 4-yr nursing schools there was a relatively higher credits ratio of general education curriculum and selective courses than in 3-yr nursing schools. 3) In 4-yr nursing schools variety of courses was relatively higher than 3-yr nursing schools. 4) In 4-yr nursing schools, operating conditions were relatively better (number of tenure professors, ratio of professors to students, Identification of exclusive organization in charge of the general education curriculum) for the general education curriculum than 3-yr nursing schools. Conclusion: The results identify significant differences in the general education curriculum of 4-yr and 3-yr nursing schools in Korea, indicating that 3-yr nursing schools should make efforts to improve the good quality of general education curriculum.

• 한국산학기술학회논문지
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• 제21권4호
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• pp.525-537
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• 2020

본 연구 목적은 향후 시뮬레이션 기반 운영 과정에 대한 과학적 근거를 제공하기 위해 연구 결과를 종합적으로 검토함으로써 Jeffries 이론적 프레임 워크를 기반으로 한국의 간호 교육에서 시뮬레이션 기반 운영 프로세스의 현재 상태와 특성을 확인하는 것이다. 본 연구는 2017년 11월까지 "시뮬레이션"과"간호"라는 용어를 사용하여 한국 교육연구 정보원, 국립 도서관, 한국학 정보원 서비스 시스템, 국립 디지털 과학 도서관, 한국 과학 기술 정보원, KOREAMED, 한국 의료 데이터베이스 등 8개의 데이터베이스에서 한국어로 출간된 연구들을 검색했다 연구결과 16개의 연구가 확인, 검토 및 평가되었다. 문헌은 연구의 일반적인 특성, 운영 방법, 교수학습방법, 참여자 특성, 결과변수 및 이론적 틀이라는 주제로 분류되었습니다. 본 연구에서 분석한 한국의 간호 교육 시뮬레이션 과정은 NLN Jeffries 시뮬레이션 이론적기틀에서 제안된 주요 개념을 완전히 반영하지는 않았다. 추후 시뮬레이션 프로그램 개발자는 시뮬레이션 효율성을 향상시키기 위해 필수 구성 요소를 확인하고 다양한 전략을 고려하여 통합해야 할 것이다.

• Journal of Plant Biotechnology
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• 제43권3호
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• pp.367-375
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• 2016

국내에서 육성된 옥수수 90 품종 및 자식 계통에 대하여 microsatellite 마커를 활용하여 DNA 프로파일 데이터베이스를 구축한 다음 공시품종에 따른 유전적 유사도 분석 및 품종식별력 검정에 대한 연구를 수행하였다. 옥수수 90품종을 100개의 microsatellite 마커로 검정하고 대립유전자의 패턴이 우수하고 다형성 정도가 높은 13개를 선정하여 분석하였을 때 대립유전자의 수는 5 ~ 24개까지 다양하게 분포하였고 평균 대립유전자의 수는 13.69개로 높았다. PIC 값의 경우도 0.716 ~ 0.942 범위에 속하였고 평균값은 0.865로 아주 높았다. 옥수수 90품종 및 계통에 대하여 UPGMA 분석에 의한 계통도를 작성하였을 때, 옥수수의 품종 유형 및 품종 육성 계보에 따라 5개의 대그룹으로 나누어졌다. 본 연구에서 구축됨 옥수수 자식계통 및 품종별 microsatellite DNA 프로파일 데이터베이스는 신품종과 기 육성된 품종과 유전적 유사도 분석이 가능하기 때문에 품종보호출원시 대조품종 선정 및 품종진위성과 관련된 종자분쟁에 매우 유용하게 활용될 수 있을 것이다.